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ARS Home » Midwest Area » Ames, Iowa » National Animal Disease Center » Ruminant Diseases and Immunology Research » People & Locations » John Lippolis

John D Lippolis
Ruminant Diseases and Immunology Research
Research Molecular Biologist

Phone: (515) 337-7446
Fax: (515) 337-7149
Room 1133

1920 DAYTON AVE
AMES, IA 50010
Projects
Non-antibiotic Approaches to Control Mastitis
In-House Appropriated (D)
  Accession Number: 432018
Cell Based Therapeutic Approach for Immunomodulation of Inflammatory Response for a Better Outcome in Dairy Animals with Mastitis
Reimbursable Cooperative Agreement (R)
  Accession Number: 435672

Publications (Clicking on the reprint icon Reprint Icon will take you to the publication reprint.)
Expression of viral microRNAs in serum and white blood cells of cows exposed to bovine leukemia virus Reprint Icon - (Peer Reviewed Journal)
Casas, E., Ma, H., Lippolis, J.D. 2020. Expression of viral microRNAs in serum and white blood cells of cows exposed to bovine leukemia virus. Frontiers in Veterinary Science. 7. https://doi.org/10.3389/fvets.2020.536390.
Characterization of a persistent, treatment-resistant, Staphylococcus aureus infection causing chronic mastitis in a Holstein dairy cow Reprint Icon - (Peer Reviewed Journal)
Putz, E.J., Palmer, M.V., Ma, H., Casas, E., Reinhardt, T.A., Lippolis, J.D. 2020. Characterization of a persistent, treatment-resistant, Staphylococcus aureus infection causing chronic mastitis in a Holstein dairy cow. BioMed Central (BMC) Veterinary Research. 16:336. https://doi.org/10.1186/s12917-020-02528-8.
Dataset of bovine mammary gland dry secretion proteome from the end of lactation through day 21 of the dry period Reprint Icon - (Peer Reviewed Journal)
Reinhardt, T.A., Lippolis, J.D. 2020. Dataset of bovine mammary gland dry secretion proteome from the end of lactation through day 21 of the dry period. Data in Brief. https://doi.org/10.1016/j.dib.2020.105954.
Identification of a reliable fixative solution to preserve complex architecture of bacterial biofilms for scanning electron microscopy evaluation Reprint Icon - (Peer Reviewed Journal)
Dassanayake, R.P., Falkenberg, S.M., Stasko, J.A., Shircliff, A.L., Lippolis, J.D., Briggs, R.E. 2020. Identification of a reliable fixative solution to preserve complex architecture of bacterial biofilms for scanning electron microscopy evaluation. PLoS One. 15(5):e0233973. https://doi.org/10.1371/journal.pone.0233973.
Characterization of bovine mammary gland dry secretions and their proteome from the end of lactation through day 21 of the dry period Reprint Icon - (Peer Reviewed Journal)
Reinhardt, T.A., Lippolis, J.D. 2020. Characterization of bovine mammary gland dry secretions and their proteome from the end of lactation through day 21 of the dry period. Journal of Proteomics. https://doi.org/10.1016/j.jprot.2020.103831.
Genome sequence of a chronic Staphylococcus aureus isolated from a dairy cow that was non-responsive to antibiotic treatment Reprint Icon - (Peer Reviewed Journal)
Lippolis, J.D., Putz, E.J., Ma, H., Alt, D.P., Casas, E., Reinhardt, T.A. 2020. Genome sequence of a chronic Staphylococcus aureus strain isolated from a dairy cow that was non-responsive to antibiotic treatment. Microbiology Resource Announcements. 9(20):e00206-20. https://doi.org/10.1128/MRA.00206-20.
Lactation stage impacts the glycolytic function of bovine CD4+ T cells during ex vivo activation Reprint Icon - (Peer Reviewed Journal)
Eder, J.M., Gorden, P.J., Lippolis, J.D., Reinhardt, T.A., Sacco, R.E. 2020. Lactation stage impacts the glycolytic function of bovine CD4+ T cells during ex vivo activation. Nature Scientific Reports. 10(4045). https://doi.org/10.1038/s41598-020-60691-2.
MicroRNA profiles of dry secretions through the first three weeks of the dry period from Holstein cows Reprint Icon - (Peer Reviewed Journal)
Putz, E.J., Putz, A.M., Jeon, H., Lippolis, J.D., Ma, H., Reinhardt, T.A., Casas, E. 2019. MicroRNA profiles of dry secretions through the first three weeks of the dry period from Holstein cows. Scientific Reports. 9(19658). https://doi.org/10.1038/s41598-019-56193-5.
The Queen Conch (Lobatus gigas) proteome: a valuable tool for biological studies in marine gastropods Reprint Icon - (Peer Reviewed Journal)
Dominguez-Perez, D., Lippolis, J.D., Dennis, M., Miller, B., Tiley, K., Vasconcelos, V., De Almeida, A.M., Campos, A. 2019. The Queen Conch (Lobatus gigas) proteome: a valuable tool for biological studies in marine gastropods. The Protein Journal. 38:628-639. https://doi.org/10.1007/s10930-019-09857-0.
Differential phenotype of immune cells in blood and milk following pegylated granulocyte colony stimulating factor (PEG-gCSF) therapy during a chronic Staphylococcus aureus infection in lactating Holsteins Reprint Icon - (Peer Reviewed Journal)
Powell, E.J., Eder, J.M., Reinhardt, T.A., Sacco, R.E., Casas, E., Lippolis, J.D. 2019. Differential phenotype of immune cells in blood and milk following pegylated granulocyte colony stimulating factor (PEG-gCSF) therapy during a chronic Staphylococcus aureus infection in lactating Holsteins. Journal of Dairy Science. 102(10):9268-9284. https://doi.org/10.3168/jds.2019-16448.
Bovine NK-lysin-derived peptide NK2A demonstrates immunomodulatory activity on cattle leukocytes - (Abstract Only)
Avian intestinal mucus modulates Campylobacter jejuni gene expression in a host-specific manner Reprint Icon - (Peer Reviewed Journal)
Looft, T.P., Cai, G., Choudhury, B., Lai, L.X., Lippolis, J.D., Reinhardt, T.A., Sylte, M.J., Casey, T.A. 2019. Avian intestinal mucus modulates Campylobacter jejuni gene expression in a host-specific manner. Frontiers in Microbiology. 9:3215. https://doi.org/10.3389/fmicb.2018.03215.
Membrane and cytoplasmic proteins of Mycobacterium avium subspecies paratuberculosis that bind to novel monoclonal antibodies Reprint Icon - (Peer Reviewed Journal)
Bannantine, J.P., Stabel, J.R., Lippolis, J.D., Reinhardt, T.A. 2018. Membrane and cytoplasmic proteins of Mycobacterium avium subspecies paratuberculosis that bind to novel monoclonal antibodies. Microorganisms. 6(4):127. https://doi.org/10.3390/microorganisms6040127.
Immunoproteomic detection and in vivo screening of protein targets for control of Glasser's disease in pigs - (Abstract Only)
Hau, S.J., Eberle, K.C., Nally, J.E., Lippolis, J.D., Mou, K.T., Brockmeier, S. 2018. Immunoproteomic detection and in vivo screening of protein targets for control of Glasser's disease in pigs. MedVet Pathogens 2018 5th Prato Conference on Animnal Bacterial Pathogens, October 8-11, 2018, Prato, Italy. Abstract No. 48.
Symposium review: Omics in dairy and animal science--Promise, potential, and pitfalls Reprint Icon - (Peer Reviewed Journal)
Lippolis, J.D., Powell, E.J., Reinhardt, T.A., Thacker, T.C., Casas, E. 2019. Symposium review: omics in dairy and animal science-promise, potential, and pitfalls. Journal of Dairy Science. 102(5):4741-4754. https://doi.org/10.3168/jds.2018-15267.
The effect of pegylated granulocyte colony stimulating factor therapy against experimental mastitis in lactating Holsteins Reprint Icon - (Peer Reviewed Journal)
Powell, E.J., Reinhardt, T.A., Casas, E., Lippolis, J.D. 2018. The effect of pegylated granulocyte colony-stimulating factor treatment prior to experimental mastitis in lactating Holsteins. Journal of Dairy Science. 101(9):8182-8193. https://doi.org/10.3168/jds.2018-14550.
Escherichia coli O157:H7 and rectoanal junction cell interactome - (Abstract Only)
Preliminary analysis of the proteome of exhaled breath condensate in bottlenose dolphins (Tursiops truncatus) Reprint Icon - (Peer Reviewed Journal)
Bergfelt, D.R., Lippolis, J.D., Vandenplas, M., Davis, S., Miller, B.A., Madan, R., Kline, M., Martinez, M., Sanchez-Okrucky, R., De Almeida, A.M. 2018. Preliminary analysis of the proteome of exhaled breath condensate in bottlenose dolphins (Tursiops truncatus) . Aquatic Mammals. 44/256-266. https://doi.org/10.1578/am.44.3.2018.256.
Considerations for farm animal proteomics experiments:an introductory view gel based versus non-gel based approaches. Reprint Icon ()
Lippolis, J.D., Nally, J.E. 2018. Considerations for farm animal proteomics experiments: An introductory view gel based versus non-gel based approaches. In: de Almeida A., Eckersall D., Miller I., editors. Proteomics in Domestic Animals: from Farm to System Biology. Basel, Switzerland: Springer International Publishing AG. p. 7-16. https://doi.org/10.1007/978-3-319-69682-9_2.
Genomic and transcriptomic analysis of Escherichia coli strains associated with persistent and transient bovine mastitis and the role of colanic acid Reprint Icon - (Peer Reviewed Journal)
Lippolis, J.D., Holman, D.B., Brunelle, B.W., Thacker, T.C., Bearson, B.L., Reinhardt, T.A., Sacco, R.E., Casey, T. 2017. Genomic and transcriptomic analysis of Escherichia coli strains associated with persistent and transient bovine mastitis and the role of colanic acid. Infection and Immunity. 86(1):e00566-17. https://doi.org/10.1128/IAI.00566-17.
Genome sequences of Escherichia coli strains that cause persistent and transient mastitis Reprint Icon - (Peer Reviewed Journal)
Thacker, T.C., Lippolis, J.D., Brunelle, B.W., Casey, T., Reinhardt, T.A., Sacco, R.E., Holman, D.B. 2017. Genome sequences of Escherichia coli strains that cause persistent and transient mastitis. Genome Announcements. 5(34):e00775-17. https://doi.org/10.1128/genomeA.00775-17.
The quest for cross protective factors of Haemophilus parasuis using 2-D gel electrophoresis - (Abstract Only)
Vitamin D status of dairy cattle: Outcomes of current practices in the dairy industry Reprint Icon - (Peer Reviewed Journal)
Nelson, C.D., Lippolis, J.D., Reinhardt, T.A., Sacco, R.E., Powell, T.L., Drewnoski, M.E., O'Neill, M., Beitz, D.C., Weiss, W.P. 2016. Vitamin D status of dairy cattle: Outcomes of current practices in the dairy industry. Journal of Dairy Science. 99:10150-10160.
Differential gene expression of three mastitis-causing Escherichia coli strains grown under planktonic, swimming, and swarming culture conditions Reprint Icon - (Peer Reviewed Journal)
Lippolis, J.D., Brunelle, B.W., Reinhardt, T.A., Sacco, R.E., Thacker, T.C., Looft, T.P., Casey, T. 2016. Differential gene expression of three mastitis-causing Escherichia coli strains grown under planktonic, swimming, and swarming culture conditions. mSystems. 1(4):e00064-16.
The goat (Capra hircus) mammary gland secretory tissue proteome as influenced by weight loss: A study using label free proteomics Reprint Icon - (Peer Reviewed Journal)
Hernandez-Castellano, L.E., Ferreira, A.M., Nanni, P., Grossmann, J., Arguello, A., Capote, J., Cai, G., Lippolis, J.D., Castro, N., Almeida, A.M. 2016. The goat (Capra hircus) mammary gland secretory tissue proteome as influenced by weight loss: A study using label free proteomics. Journal of Proteomics. 145:60-69. doi: 10.1016/j.jprot.2016.03.030.
Comparative analysis of Escherichia coli O157 growth and protein-expression, in vitro and in vivo, in rumen fluid of cattle - (Abstract Only)
Kudva, I.T., Casey, T., Lippolis, J.D., Trachsel, J., Allen, H.K. 2016. Comparative analysis of Escherichia coli O157 growth and protein-expression, in vitro and in vivo, in rumen fluid of cattle [abstract]. American Society for Microbiology Microbe, June 16 - 20, 2016, Boston, Massachusetts. p. 567.
Quantification of bovine oxylipids during intramammary Streptococcus uberis infection - (Peer Reviewed Journal)
Ryman, V.E., Pighetti, G.M., Lippolis, J.D., Gandy, J.C., Applegate, C.M., Sordillo, L.M. 2015. Quantification of bovine oxylipids during intramammary Streptococcus uberis infection. Prostaglandins & Other Lipid Mediators. doi: 10.1016/j.prostaglandins.2015.09.006.
Composition and potency characterization of Mycobacterium avium subsp. paratuberculosis purified protein derivatives Reprint Icon - (Peer Reviewed Journal)
Capsel, R.T., Thoen, C.O., Reinhardt, T.A., Lippolis, J.D., Olsen, R., Stabel, J.R., Bannantine, J.P. 2016. Composition and potency characterization of Mycobacterium avium subsp. paratuberculosis purified protein derivatives. Veterinary Microbiology. 11(5):e0154685. doi: 10.1371/journal.pone.0154685.
Multiple ß-defensin genes are upregulated by the vitamin D pathway in cattle - (Peer Reviewed Journal)
Merriman, K.E., Kweh, M.F., Powell, J.L., Lippolis, J.D., Nelson, C.D. 2015. Multiple ß-defensin genes are upregulated by the vitamin D pathway in cattle. The Journal of Steroid Biochemistry and Molecular Biology. doi: 10.1016/j.jsbmb.2015.08.002.
Seasonal variation in vitamin D status of beef cattle reared in the central United States Reprint Icon - (Peer Reviewed Journal)
Casas, E., Lippolis, J.D., Kuehn, L.A., Reinhardt, T.A. 2015. Seasonal variation in vitamin D status of beef cattle reared in the central United States. Domestic Animal Endocrinology. 52:71-74. DOI: 10.1016/j.domaniend.2015.03.003.
Sequence analysis of a bitter taste receptor gene repertoires in different ruminant species Reprint Icon - (Peer Reviewed Journal)
Ferreira, A.M., Marques, A.T., Bhide, M., Cubric-Curik, V., Hollung, K., Knight, C.H., Raundrup, K., Lippolis, J.D., Palmer, M.V., Sales-Baptista, E., Araujo, S.S., De Almeida, A.M. 2015. Sequence analysis of a bitter taste receptor gene repertoires in different ruminant species. PLoS One. 10(6):e0129740. doi: 10.1371/journal.pone.0129740.
Changes in the proteome of Mastitis-causing escherichia coli strains that affect pathogenesis - (Abstract Only)
Proteomic analysis reveals protein expression differences in Escherichia coli strains associated with persistent versus transient mastitis Reprint Icon - (Peer Reviewed Journal)
Lippolis, J.D., Brunelle, B.W., Reinhardt, T.A., Sacco, R.E., Nonnecke, B.J., Dogan, B., Simpson, K., Schukken, Y. 2014. Proteomic analysis reveals protein expression differences in Escherichia coli strains associated with persistent versus transient mastitis. Journal of Proteomics. 108:373-381. DOI: 10.1016/j.jprpt.2014.06.008.
Acute phase response elicited by experimental bovine diarrhea virus (BVDV) infection is associated with decreased vitamin D and E status of vitamin-replete preruminant calves Reprint Icon - (Peer Reviewed Journal)
Nonnecke, B.J., McGill, J.L., Ridpath, J.F., Sacco, R.E., Lippolis, J.D., Reinhardt, T.A. 2014. Acute phase response elicited by experimental bovine diarrhea virus (BVDV) infection is associated with decreased vitamin D and E status of vitamin-replete preruminant calves. Journal of Dairy Science. 97(9):5566-5579. DOI: 10.3168/jds.2014-8293.
MicroRNA content in milk exosomes as a phenotypic indicator of Staphylococcus aureus infection in the bovine mammary gland - (Abstract Only)
Jiajie, S., Kshama, A., Schroeder, S.G., Reinhardt, T.A., Lippolis, J.D., Sonstegard, T.S. 2014. MicroRNA content in milk exosomes as a phenotypic indicator of Staphylococcus aureus infection in the bovine mammary gland. BARC Poster Day, Poster 61, pp. 73.
Computational analysis of bovine milk exosomal miRNAs profiles derived from uninfected and Streptococcus uberis infected mammary gland - (Abstract Only)
Aswath, K., Jiajie, S., Schroeder, S.G., Reinhardt, T.A., Lippolis, J.D., Sonstegard, T.S. 2014. Computational analysis of bovine milk exosomal miRNAs profiles derived from uninfected and Streptococcus uberis infected mammary gland. BARC Poster Day, Poster 62, pp. 74.
Proteins facilitating Escherichia coli O157 persistence at the bovine recto-anal junction (RAJ) squamous epithelial cells - (Abstract Only)
Kudva, I.T., Lippolis, J.D., John, M. 2014. Proteins facilitating Escherichia coli O157 persistence at the bovine recto-anal junction (RAJ) squamous epithelial cells [abstract]. American Society for Microbiology. Abstract No. 2576.
The Escherichia coli O157:H7 bovine rumen fluid proteome reflects adaptive bacterial responses Reprint Icon - (Peer Reviewed Journal)
Kudva, I.T., Stanton, T.B., Lippolis, J.D. 2014. The Escherichia coli O157:H7 bovine rumen fluid proteome reflects adaptive bacterial responses. BMC Microbiology. 14:48. DOI: 10.1186/1471-2180-14-48.
The Ca2+/H+ antiporter TMEM165 expression, localization in the developing, lactating and involuting mammary gland parallels the secretory pathway Ca2+ATPase (SPCA1) Reprint Icon - (Peer Reviewed Journal)
Reinhardt, T.A., Lippolis, J.D., Sacco, R.E. 2014. The Ca2+/H+ antiporter TMEM165 expression, localization in the developing, lactating and involuting mammary gland parallels the secretory pathway Ca2+ATPase (SPCA1). Biochemical and Biophysical Research Communications. 445(2):417-421.
MicroRNA regulation of bovine monocyte inflammatory and metabolic networks in an in vivo infection model Reprint Icon - (Peer Reviewed Journal)
Lawless, N., Reinhardt, T.A., Bryan, K., Baker, M., Pesch, B.A., Zimmerman, D.R., Zuelke, K.A., Sonstegard, T.S., O'Farrelly, C., Lippolis, J.D., Lynn, D.J. 2014. MicroRNA regulation of bovine monocyte inflammatory and metabolic networks in an in vivo infection model. Genes, Genomes, Genetics. 4(6):957-971. DOI: 10.1534/g3.113.009936.
Seasonal variation in vitamin D status of beef cattle reared in the midwest and Fed to NRC recommendations. - (Abstract Only)
Neonatal calf infection with respiratory syncytial virus: drawing parallels to the disease in human infants ()
Sacco, R.E., McGill, J.L., Palmer, M.V., Lippolis, J.D., Reinhardt, T.A., Nonnecke, B.J. 2014. Neonatal calf infection with respiratory syncytial virus: drawing parallels to the disease in human infants. In: Rosenberg, H.F., Domachowske, J.B., editors. Pneumoviruses and Metapneumoviruses. Basel, Switzerland: MDPI AG. p. 46-69.
Proteomic differences between Escherichia coli strains that cause transient versus persistent intramammary infections [abstract] - (Abstract Only)
A genome-wide association study identified CYP2J2 as a major gene controlling serum vitamin D in beef cattle Reprint Icon - (Peer Reviewed Journal)
Casas, E., Leach, R.J., Reinhardt, T.A., Thallman, R.M., Lippolis, J.D., Bennett, G.L., Kuehn, L.A. 2013. A genome-wide association study identified CYP2J2 as a major gene controlling serum vitamin D in beef cattle. Journal of Animal Science. 91:3549-3556.
Proteomic analysis of Escherichia coli O157 cultured in bovine rumen fluid - (Abstract Only)
Kudva, I.T., Stanton, T.B., Lippolis, J.D. 2013. Proteomic analysis of Escherichia coli O157 cultured in bovine rumen fluid [abstract]. 113th General Meeting, American Society for Microbiology. p. 190.
The need for agriculture phenotyping: “Moving from genotype to phenotype” Reprint Icon - (Peer Reviewed Journal)
Boggess, M.V., Lippolis, J.D., Hurkman II, W.J., Fagerquist, C.K., Briggs, S.P., Gomes, A.V., Righetti, P., Bala, K. 2013. The need for agriculture phenotyping: “Moving from genotype to phenotype”. Journal of Proteomics. doi: http://dx.doi.org/10.1016/j.jptot.2013.3.021.
A genome-wide association study identified CYP2J2 as a major gene controlling serum Vitamin D status in beef cattle - (Abstract Only)
Casas, E., Leach, R.J., Reinhardt, T.A., Thallman, R.M., Lippolis, J.D., Bennett, G.L., Kuehn, L.A. 2013. A genome-wide association study identified CYP2J2 as a major gene controlling serum Vitamin D status in beef cattle. Journal of Animal Science Supplement. Vol.91(Supplement 2):62.Abstract No. 0185.
Bovine milk proteome: Quantitative changes in normal milk exosomes, milk fat globule membranes and whey proteomes resulting from Staphylococcus aureus mastitis Reprint Icon - (Peer Reviewed Journal)
Reinhardt, T.A., Sacco, R.E., Nonnecke, B.J., Lippolis, J.D. 2013. Bovine milk proteome: Quantitative changes in normal milk exosomes, milk fat globule membranes and whey proteomes resulting from Staphylococcus aureus mastitis. Journal of Proteomics. 82:141-154.
Differential chemokine and cytokine production by neonatal bovine gamma delta T-cell subsets in response to viral toll-like receptor agonists and in vivo respiratory syncytial virus infection Reprint Icon - (Peer Reviewed Journal)
McGill, J.L., Nonnecke, B.J., Lippolis, J.D., Reinhardt, T.A., Sacco, R.E. 2013. Differential chemokine and cytokine production by neonatal bovine gamma delta T-cell subsets in response to viral toll-like receptor agonists and in vivo respiratory syncytial virus infection. Immunology. 139(2):227-244.
Neonatal calf infection with respiratory syncytial virus: drawing parallels to the disease in human infants ()
Sacco, R.E., McGill, J.L., Palmer, M.V., Lippolis, J.D., Reinhardt, T.A., Nonnecke, B.J. 2012. Neonatal calf infection with respiratory syncytial virus: drawing parallels to the disease in human infants. Viruses. 4(12):3731-3753.
Characterization of carotenoid-protein complexes and gene expression analysis associated with carotenoid sequestration in pigmented cassava (Manihot esculenta Crantz) storage root - (Peer Reviewed Journal)
Carvalho, L.J., Lippolis, J.D., Chen, S., De Souza, C.R., Vieria, E.A., Anderson, J.V. 2012. Characterization of carotenoid-protein complexes and gene expression analysis associated with carotenoid sequestration in pigmented cassava (Manihot esculenta Crantz) storage root. The Open Biochemistry Journal. 6:116-130.
Vitamin D signaling in the bovine immune system: A model for understanding human vitamin D requirements - (Peer Reviewed Journal)
Nelson, C.D., Reinhardt, T.A., Lippolis, J.D., Sacco, R.E., Nonnecke, B.J. 2012. Vitamin D signaling in the bovine immune system: A model for understanding human vitamin D requirements. Nutrients. 4(3):181-196.
Differential expression of cytokines in response to respiratory syncytial virus infection of calves with high or low circulating 25-hydroxyvitamin D3 - (Peer Reviewed Journal)
Sacco, R.E., Nonnecke, B.J., Palmer, M.V., Waters, W.R., Lippolis, J.D., Reinhardt, T.A. 2012. Differential expression of cytokines in response to respiratory syncytial virus infection of calves with high or low circulating 25-hydroxyvitamin D3. PLoS One. 7(3):e33074. Available: http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0033074.
Bovine milk exosome proteome - (Peer Reviewed Journal)
Reinhardt, T.A., Lippolis, J.D., Nonnecke, B.J., Sacco, R.E. 2012. Bovine milk exosome proteome. Journal of Proteomics. 75(5):1486-1492.
Treatment of an intramammary bacterial infection with 25-hydroxyvitamin D3 - (Peer Reviewed Journal)
Lippolis, J.D., Reinhardt, T.A., Sacco, R.E., Nonnecke, B.J., Nelson, C.D. 2011. Treatment of an intramammary bacterial infection with 25-hydroxyvitamin D3. PLoS One. 6(10):e25479. Available: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3184989/pdf/pone.0025479.pdf.
Innate immune response to a bovine mastitis pathogen profiled in milk and blood monocytes using a systems biology approach - (Abstract Only)
Regulation of Mycobacterium-specific mononuclear cell responses by 25-hydroxyvitamin D3 - (Peer Reviewed Journal)
Nelson, C.D., Nonnecke, B.J., Reinhardt, T.A., Waters, W.R., Beitz, D.C., Lippolis, J.D. 2011. Regulation of Mycobacterium-specific mononuclear cell responses by 25-hydroxyvitamin D3. PLoS One. 6(6):e21674. Available: http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0021674.
Utility, Limitations, and Promise of Proteomics in Animal Science - (Peer Reviewed Journal)
Lippolis, J.D., Reinhardt, T.A. 2010. Utility, limitations, and promise of proteomics in animal science. Veterinary Immunology and Immunopathology. 138(4):241-251.
In vivo activation of the intracrine vitamin D pathway in innate immune cells and mammary tissue during a bacterial infection - (Peer Reviewed Journal)
Nelson, C.D., Reinhardt, T.A., Beitz, D.C., Lippolis, J.D. 2010. In vivo activation of the intracrine vitamin D pathway in innate immune cells and mammary tissue during a bacterial infection. PLoS One. 5(11):e15469. Available: http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0015469.
Methods and Approaches to Mass Spectroscopy Based Protein Identification ()
Lippolis, J.D., Reinhardt, T.A. 2011. Methods and approaches to mass spectroscopy based protein identification. In: Whitfield, P.D. Eckersall, D., editors. Methods in animal proteomics. West Sussex, UK: Wiley-Blackwell. p. 77-102.
Prevalence of Subclinical Hypocalcemia in Dairy Herds - (Peer Reviewed Journal)
Reinhardt, T.A., Lippolis, J.D., Mccluskey, B.J., Goff, J.P., Horst, R.L. 2011. Prevalence of Subclinical Hypocalcemia in Dairy Herds. The Veterinary Journal. 188:122-124.
1,25-Dihydroxyvitamin D3 Enhances Innate Immune Responses of Bovine Mammary Epithelial Cells that are Triggered by Toll-like Receptor Signaling - (Abstract Only)
Nelson, C.D., Reinhardt, T.A., Beitz, D.C., Lippolis, J.D. 2010. 1,25-Dihydroxyvitamin D3 Enhances Innate Immune Responses of Bovine Mammary Epithelial Cells that are Triggered by Toll-like Receptor Signaling [abstract]. Midwest Branch of the American Dairy Science Association. p. 30.
Vitamin D Signaling in the Bovine Mammary Gland is Part of the Innate Immune Response to Bacterial Pathogens - (Abstract Only)
Nelson, C.D., Reinhardt, T.A., Lippolis, J.D. 2010. Vitamin D Signaling in the Bovine Mammary Gland is Part of the Innate Immune Response to Bacterial Pathogens [abstract]. National Mastitis Council. p. 196.
Modulation of the Bovine Innate Immune Response by Production of 1alpha,25-Dihydroxyvitamin D3 in Bovine Monocytes - (Peer Reviewed Journal)
Nelson, C.D., Reinhardt, T.A., Thacker, T.C., Beitz, D.C., Lippolis, J.D. 2010. Modulation of the Bovine Innate Immune Response by Production of 1alpha,25-Dihydroxyvitamin D3 in Bovine Monocytes. Journal of Dairy Science. 93(3):1041-1049.
Improvement of the Innate Immune Response of Bovine Monocytes Via an Intracrine Vitamin D Signaling Pathway - (Abstract Only)
Nelson, C.D., Reinhardt, T.A., Beitz, D.C., Lippolis, J.D. 2009. Improvement of the Innate Immune Response of Bovine Monocytes Via an Intracrine Vitamin D Signaling Pathway [abstract]. Conference of Research Workers in Animal Diseases. Paper No. 94.
The Utility, Limitations, and Promise of Proteomics in Animal Science - (Abstract Only)
Expression of Inducible Nitric Oxide Synthase is Up-Regulated by Production of 1,25-Dihydroxyvitamin D3 in Bovine Monocytes in Response to Toll-Like Receptor Signaling - (Abstract Only)
Nelson, C.D., Beitz, D.C., Reinhardt, T.A., Lippolis, J.D. 2009. Expression of Inducible Nitric Oxide Synthase is Up-Regulated by Production of 1,25-Dihydroxyvitamin D3 in Bovine Monocytes in Response to Toll-Like Receptor Signaling [abstract]. American Dairy Science Association. Paper No. 84.
Understanding the Relationship Between Immunity and Dairy Cow Production ()
Lippolis, J.D. 2009. Understanding the Relationship Between Immunity and Dairy Cow Production. Hoard's Dairyman. 154 (13):481.
Proteomics of mastitis causing Escherichia coli - (Proceedings)
Lippolis, J.D., Reinhardt, T.A., Kehrli Jr, M.E. 2009. Proteomics of mastitis causing Escherichia coli. Proceedings of National Mastitis Council. 48:236-237.
Mammary gland involution is associated with rapid down regulation of major mammary Ca**2+-ATPases - (Peer Reviewed Journal)
Reinhardt, T.A., Lippolis, J.D. 2009. Mammary gland involution is associated with rapid down regulation of major mammary Ca**2+-ATPases. Biochemical and Biophysical Research Communications. 378(1):99-102.
Proteomic changes in Escherichia coli when grown in fresh milk versus laboratory media - (Peer Reviewed Journal)
Lippolis, J.D., Bayles, D.O., Reinhardt, T.A. 2009. Proteomic changes in Escherichia coli when grown in fresh milk versus laboratory media. Journal of Proteome Research. 8(1):149-158.
The promise of proteomics in animal science - (Abstract Only)
Lippolis, J.D., Reinhardt, T.A. 2008. The promise of proteomics in animal science [abstract]. 2008 Joint Annual Meeting of the American Dairy Science Association/American Society of Animal Science, July 7-11, 2008, Indianapolis, Indiana. 2008 CD-ROM.
Centennial Paper: Proteomics in animal science - (Peer Reviewed Journal)
Lippolis, J.D., Reinhardt, T.A. 2008. Centennial Paper: Proteomics in animal science. Journal of Animal Science. 86(9):2430-2441.
Developmental changes in the milk fat globule membrane proteome during the transition from colostrum to milk - (Peer Reviewed Journal)
Reinhardt, T.A., Lippolis, J.D. 2008. Developmental changes in the milk fat globule membrane proteome during the transition from colostrum to milk. Journal of Dairy Science. 91(6):2307-2318.
Toll-like receptor signaling increases production of 1,25-dihydroxyvitamin D3 in bovine macrophages - (Abstract Only)
Nelson, C.D., Beitz, D.C., Reinhardt, T.A., Lippolis, J.D. 2008. Toll-like receptor signaling increases production of 1,25-dihydroxyvitamin D3 in bovine macrophages [abstract). Experimental Biology 2008. p. 188.
Immunological signaling networks: Integrating the body's immune response - (Peer Reviewed Journal)
Lippolis, J.D. 2008. Immunological signaling networks: integrating the body's immune response. Journal of Animal Science. 86(14 Suppl):E53-E63.
Autocrine signaling mechanism of vitamin D in the bovine innate immune response - (Abstract Only)
Nelson, C.D., Beitz, D.C., Reinhardt, T.A., Lippolis, J.D. 2008. Autocrine signaling mechanism of vitamin D in the bovine innate immune response [abstract]. 2008 American Dairy Science Association, American Society of Animal Science, Midwestern Section, Annual Meeting. p. 37.
Adaptive changes in protein expression in Escherichia coli as a consequence of growth in milk - (Abstract Only)
Lippolis, J.D., Reinhardt, T.A. 2007. Adaptive changes in protein expression in Escherichia coli as a consequence of growth in milk [abstract]. Conference of Research Workers in Animal Diseases. p. 123.
Cumulative physiological events modulate the inflammatory response of the bovine udder to Escherichia coli infections around parturition - (Proceedings)
Burvenich, C., Bannerman, D.D., Lippolis, J.D., Peelman, L., Nonnecke, B.J., Kehrli Jr, M.E., Paape, M.J. 2007. Cumulative physiological events modulate the inflammatory response of the bovine udder to Escherichia coli infections around parturition. Proceedings of the Heifer Mastitis Conference. pp. 75-76. June 24-26, 2007. Ghent, Belgium. . Meeting Abstract.
Developmental changes in milk fat globule membrane proteome expression during the transition from colostrum to milk - (Abstract Only)
Reinhardt, T.A., Lippolis, J.D. 2007. Developmental changes in milk fat globule membrane proteome expression during the transition from colostrum to milk [abstract]. 2007 Joint Meeting-American Dairy Science Association, Poultry Science Association, Asociacion Mexicana de Produccion Animal, American Society of Animal Sciences. p. 58.
Changes in protein expression in Escherichia coli as a consequence of growth in milk whey - (Abstract Only)
Lippolis, J.D., Reinhardt, T.A. 2007. Changes in protein expression in Escherichia coli as a consequence of growth in milk whey [abstract]. 2007 Joint Meeting-American Dairy Science Association, Poultry Science Association, Asociacion Mexicana de Produccion Animal, American Society of Animal Sciences. p. 8.
Cumulative physiological events influence the inflammatory response of the bovine udder to Escherichia coli infections during the transition period ()
Burvenich, C., Bannerman, D.D., Lippolis, J.D., Peelman, L., Nonnecke, B.J., Kehrli, Jr., M.E., Paape, M.J. 2007. Cumulative physiological events influence the inflammatory response of the bovine udder to Escherichia coli infections during the transition period. Journal of Dairy Science. 90(E.Suppl.):E39-E54.
Proteome and Differential Expression Analysis of Membrane and Cytosolic Proteins from Mycobacterium avium subsp. paratuberculosis Strains K-10 and 187. - (Peer Reviewed Journal)
Radosevich, T.J., Reinhardt, T.A., Lippolis, J.D., Bannantine, J.P., Stabel, J.R. 2007. Proteome and Differential Expression Analysis of Membrane and Cytosolic Proteins from Mycobacterium avium subsp. paratuberculosis Strains K-10 and 187. Journal of Bacteriology. 189(3):1109-1117.
IDENTIFICATION OF DIFFERENCES IN THE PROTEIN CONTENT OF MYCOBACTERIUM AVIUM SUBSPECIES PARATUBERCULOSIS PURIFIED PROTEIN DERIVATIVE PREPARATIONS WITH DIFFERENT SKIN TEST SPECIFICITIES - (Abstract Only)
Paustian, M., Robbe Austerman, S., Lippolis, J.D., Stabel, J.R., Bannantine, J.P. 2006. Identification of Differences in the Protein Content of Mycobacterium avium subspecies paratuberculosis Purified Protein Derivative Preparations with Different Skin Test Specificities [abstract]. American Society for Microbiology. Paper No. U-016.
PROTEOME AND DIFFERENTIAL EXPRESSION ANALYSIS OF MEMBRANE AND CYTOSOLIC PROTEIN FROM MYCOBACTERIUM AVIUM SUBS PARATUBERCULOSIS STRAINS K10 AND 187 - (Abstract Only)
Radosevich, T.J., Stabel, J.R., Bannantine, J.P., Lippolis, J.D., Reinhardt, T.A. 2006. Proteome and Differential Expression Analysis of Membrane and Cytosolic Protein from Mycobacterium avium subs paratuberculosis Strains k10 and 187 [abstract]. American Society for Microbiology. p. 112.
NEUTROPHIL EXTRACELLULAR TRAP FORMATION BY BOVINE NEUTROPHILS IS NOT INHIBITED BY MILK - (Peer Reviewed Journal)
Lippolis, J.D., Reinhardt, T.A., Goff, J.P., Horst, R.L. 2006. Neutrophil extracellular trap formation by bovine neutrophils is not inhibited by milk. Veterinary Immunology and Immunopathology. 113(1-2):248-255.
BOVINE MILK FAT GLOBULE MEMBRANE PROTEOME - (Peer Reviewed Journal)
Reinhardt, T.A., Lippolis, J.D. 2006. Bovine milk fat globule membrane proteome. Journal of Dairy Research. 73(4):406-416.
BOVINE MILK FAT GLOBULE MEMBRANE PROTEOME - (Peer Reviewed Journal)
Reinhardt, T.A., Lippolis, J.D. 2006. Bovine milk fat globule membrane proteome. Journal of Dairy Research. 73(4):406-416.
CUMULATIVE PHYSIOLOGICAL EVENTS INFLUENCE THE INFLAMMATORY RESPONSE OF THE BOVINE UDDER TO E-COLI INFECTIONS DURING THE TRANSITION PERIOD - (Abstract Only)
Burvenich, C., Kehrli Jr, M.E., Paape, M.J., Bannerman, D.D., Lippolis, J.D. 2006. Cumulative physiological events influence the inflammatory response of the bovine udder to e-coli infections during the transition period. Journal of Dairy Science. 84(Suppl. 1):273.
NEUTROPHIL EXTRACELLULAR TRAP FORMATION: AN IMPORTANT NEUTROPHIL KILLING MECHANISM THAT IS NOT INHIBITED IN MILK - (Abstract Only)
Lippolis, J.D., Reinhardt, T.A., Goff, J.P., Horst, R.L. 2006. Neutrophil extracellular trap formation: an important neutrophil killing mechanism that is not inhibited in milk [abstract]. American Dairy Science Association-American Society of Animal Science 2006 Joint Annual Meeting. p. 94.
DIFFERENTIAL EXPRESSION ANALYSIS OF PROTEINS FROM NEUTROPHILS IN THE PERIPARTURIENT PERIOD AND NEUTROPHILS FROM DEXAMETHASONE-TREATED DAIRY COWS - (Peer Reviewed Journal)
Lippolis, J.D., Peterson-Burch, B.D., Reinhardt, T.A. 2006. Differential expression analysis of proteins from neutrophils in the periparturient period and neutrophils from dexamethasone-treated dairy cows. Veterinary Immunology and Immunopathology. 111(3-4):149-164.
DIFFERENTIAL EXPRESSION ANALYSIS OF IMMUNOSUPPRESSED VERSUS NORMAL NEUTROPHIL PROTEOME - (Abstract Only)
Lippolis, J.D., Reinhardt, T.A. 2005. Differential expression analysis of immunosuppressed versus normal neutrophil proteome [abstract]. American Society for Mass Spectrometry. p. 73.
THE SYSTEMIC AND LOCALIZED INNATE IMMUNE RESPONSE TO INTRAMAMMARY INFECTION WITH STREPTOCOCCUS UBERIS - (Proceedings)
Bannerman, D.D., Paape, M.J., Goff, J.P., Kimura, K., Lippolis, J.D., Hope, J.C. 2005. The systemic and localized innate immune response to intramammary infection with streptococcus uberis [abstract]. National Mastitis Council (NMC) 44th Annual Meeting Proceedings. pp. 211-212.
ENERGY AND PROTEIN EFFECTS ON THE IMMUNE SYSTEM ()
Kehrli, Jr., M.E., Neill, J.D., Burvenich, C., Goff, J.P., Lippolis, J.D., Reinhardt, T.A., Nonnecke, B.J. 2006. Energy and protein effects on the immune system. In: Sejrsen, K., Hvelplund, T., Nielsen, M.O., editors. Ruminant Physiology. Wageningen, The Netherlands: Wageningen Academic Publishers. p. 455-471.
ENERGY AND PROTEIN EFFECTS ON THE IMMUNE SYSTEM - (Proceedings)
PROTEOMIC SURVEY OF BOVINE NEUTROPHILS - (Peer Reviewed Journal)
Lippolis, J.D., Reinhardt, T.A. 2005. Proteomic survey of bovine neutrophils. Veterinary Immunology and Immunopathology. 103(1-2):53-65.
NULL MUTATION IN THE GENE ENCODING PLASMA MEMBRANE CA2+-ATPASE ISOFORM 2 IMPAIRS CALCIUM TRANSPORT INTO MILK - (Peer Reviewed Journal)
Reinhardt, T.A., Lippolis, J.D., Shull, G.E., Horst, R.L. 2004. Null mutation in the gene encoding plasma membrane CA2+-ATPase isoform 2 impairs calcium transport into milk. Journal of Biological Chemistry. 279(41):42369-42373.
INNATE IMMUNE RESPONSE TO INTRAMAMMARY INFECTION WITH SERRATIA MARCESSENS AND STREPTOCOCCUS UBERIS - (Peer Reviewed Journal)
Bannerman, D.D., Paape, M.J., Goff, J.P., Kimura, K., Lippolis, J.D., Hope, J.C. 2004. Innate immune response to intramammary infection with serratia marcessens and streptococcus uberis. Veterinary Research. 35(6):681-700.
COMPARATIVE PROTEOMIC ANALYSIS OF NORMAL AND PERIPARTURIENT BOVINE NEUTROPHILS DEMONSTRATING A REDUCTION OF MYELOPEROXIDASE DURING TRANSITION - (Abstract Only)
Lippolis, J.D., Goff, J.P., Reinhardt, T.A. 2004. Comparative proteomic analysis of normal and periparturient bovine neutrophils demonstrating a reduction of myeloperoxidase during transition [abstract]. Experimental Biology Meeting. Paper No. 776.3.
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