Richard L Hornsby
Infectious Bacterial Diseases Research
Biological Science Lab Technician (Microbiology)
Phone: (515) 337-7327
Fax: (515) 337-7149
Room 1515
1920 DAYTON AVE
AMES,
IA
50010
(Employee information on this page comes from the REE Directory. Please contact your front office staff to update the REE Directory.)
Publications
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Assessing rodents as carriers of pathogenic leptospires in the U.S. Virgin Islands: the risk to animal and public health
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Hamond, C., Browne, A.S., Hornsby, R.L., Lecount, K., Anderson, T., Stuber, T., De Wilde, L.H., Cranford, H.M., Browne, S.K., Blanchard, G., Horner, D., Taylor, M.L., Evans, M., Roth, J., Bisgard, K., Salzer, J., Schafer, I., Ellis, B., Alt, D.P., Schlater, L., Nally, J.E., Ellis, E.M. 2022. Assessing rodents as carriers of pathogenic leptospires in the U.S. Virgin Islands: the risk to animal and public health. Scientific Reports. https://doi.org/10.1038/s41598-022-04846-3.
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Antigen specific urinary immunoglobulin in reservoir hosts of leptospirosis
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Nally, J.E., Hornsby, R.L., Alt, D.P. 2021. Antigen specific urinary immunoglobulin in reservoir hosts of leptospirosis. Veterinary Immunology and Immunopathology. Vol.8 Issue 9 pg178. https://doi.org/10.3390/vetsci8090178.
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Application of CRISPR interference (CRISPRi) for gene silencing in pathogenic species of Leptospira
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Fernandes, L.G., Hornsby, R.L., Nascimento, A., Nally, J.E. 2021. Application of CRISPR interference (CRISPRi) for gene silencing in pathogenic species of Leptospira. The Journal of Visualized Experiments (JoVE). https://doi.org/10.3791/62631.
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Prevalence of Pathogenic Leptospira in Livestock in
St. Croix, U.S. Virgin Islands
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Cranford, H.M., Taylor, M., Browne, A., Alt, D.P., Anderson, T., Hammond, C., Hornsby, R.L., Lecount, K., Schlater, L., Stuber, T., DeWilde, L., Burke-France, V.J., Ellis, E.M., Nally, J.E. 2021. Exposure and carriage of pathogenic Leptospira in livestock in St. Croix, U.S. Virgin Islands. Tropical Medicine and Infectious Disease. 6(2). https://doi.org/10.3390/tropicalmed6020085.
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Distinct transcriptional profiles of Leptospira borgpetersenii serovar Hardjo strains JB197 and HB203 cultured at different temperatures
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Putz, E.J., Sivasankaran, S.K., Fernandes, L.G., Brunelle, B.W., Lippolis, J.D., Alt, D.P., Bayles, D.O., Hornsby, R.L., Nally, J.E. 2021. Distinct transcriptional profiles of Leptospira borgpetersenii serovar Hardjo strains JB197 and HB203 cultured at different temperatures. PLOS Neglected Tropical Diseases. 15(4). Article e0009320. https://doi.org/10.1371/journal.pntd.0009320.
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Genetic manipulation of pathogenic Leptospira: CRISPR interference (CRISPRi)-mediated gene silencing and rapid mutant recovery at 37C
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Fernandes, L.G., Hornsby, R.L., Nascimento, A.L., Nally, J.E. 2021. Genetic manipulation of pathogenic Leptospira: CRISPR interference (CRISPRi)-mediated gene silencing and rapid mutant recovery at 37C. Scientific Reports. 11. https://doi.org/10.1038/s41598-021-81400-7.
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Isolation and propagation of leptospires at 37C directly from the mammalian host
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Hornsby, R.L., Alt, D.P., Nally, J.E. 2020. Isolation and propagation of leptospires at 37 degree C directly from the mammalian host. Scientific Reports. 10(9620). https://doi.org/10.1038/s41598-020-66526-4.
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Bovine immune response to leptospiral antigen in different novel adjuvants and vaccine delivery platforms
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Wilson-Welder, J.H., Boggiatto, P.M., Nally, J.E., Wafa, E.I., Alt, D.P., Hornsby, R.L., Frank, A.T., Jones, D.E., Olsen, S.C., Bowden, N.B., Salem, A.K. 2020. Bovine immune response to leptospiral antigen in different novel adjuvants and vaccine delivery platforms. Vaccine. 38(18):3464-3473. https://doi.org/10.1016/j.vaccine.2020.02.086.
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Phenotypic and proteomic characterization of treponemes associated with bovine digital dermatitis
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Nally, J.E., Hornsby, R.L., Alt, D.P., Whitelegge, J.P. 2019. Phenotypic and proteomic characterization of treponemes associated with bovine digital dermatitis. Veterinary Microbiology. 235:35-42. https://doi.org/10.1016/j.vetmic.2019.05.023.
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Isolation and characterization of pathogenic leptospires associated with cattle
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Nally, J.E., Hornsby, R.L., Alt, D.P., Bayles, D.O., Wilson-Welder, J.H., Bauer, N.E. 2018. Isolation and characterization of pathogenic leptospires associated with cattle. Veterinary Microbiology. 218:25-30. https://doi.org/10.1016/j.vetmic.2018.03.023.
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Inbred rats as a model to study persistent renal colonization and associated cellular immune responsiveness
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Nally, J.E., Wilson-Welder, J.H., Hornsby, R.L., Palmer, M.V., Alt, D.P. 2018. Inbred rats as a model to study persistent renal colonization and associated cellular immune responsiveness. Frontiers in Cellular and Infection Microbiology. https://doi.org/10.3389/fcimb.2018.00066.
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Emerging infectious disease implications of invasive mammalian species: the greater white-toothed shrew (Crocidura russula) is associated with a novel serovar of pathogenic Leptospira in Ireland
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Nally, J.E., Bayles, D.O., Hornsby, R.L., Arent, Z., Gilmore, C., Regan, S., McDevitt, A.D., Yearsley, J., Fanning, S., McMahon, B.J. 2016. Emerging infectious disease implications of invasive mammalian species: the greater white-toothed shrew (Crocidura russula) is associated with a novel serovar of pathogenic Leptospira in Ireland. PLOS Neglected Tropical Diseases. 10(12):e0005174.
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Interaction of bovine peripheral blood polymorphonuclear cells and Leptospira species; innate responses in the natural bovine reservoir host.
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Wilson-Welder, J.H., Frank, A.T., Hornsby, R.L., Olsen, S.C., Alt, D.P. 2016. Interaction of bovine peripheral blood polymorphonuclear cells and Leptospira species; innate responses in the natural bovine reservoir host. Frontiers in Microbiology. 7:1110.
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Role of intraocular Leptospira infections in the pathogenesis of Equine Recurrent Uveitis in the Southern United States
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Polle, F., Storey, E., Eades, S., Alt, D.P., Hornsby, R.L., Zuerner, R., Carter, R. 2014. Role of intraocular Leptospira infections in the pathogenesis of equine recurrent uveitis in the Southern United States. Journal of Equine Veterinary Science. 34(11-12):1300-1306.
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Asymptomatic and chronic carriage of Leptospira interrogans serovar Pomona in California sea lions (Zalophus californianus)
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Prager, K.C., Greig, D.J., Alt, D.P., Galloway, R.L., Hornsby, R.L., Palmer, L.J., Soper, J., Wu, Q., Zuerner, R.L., Gulland, F.M., Lloyd-Smith, J.O. 2013. Asymptomatic and chronic carriage of Leptospira interrogans serovar Pomona in California sea lions (Zalophus californianus). Veterinary Microbiology. 164(1-2):177-183.
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ISOLATION OF BRUCELLA ABORTUS STRAIN RB51 FROM VACCINATED BISON (BISON BISON)
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