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ARS Home » Northeast Area » Washington, D.C. » National Arboretum » Floral and Nursery Plants Research » People & Locations » Qi Huang
Qi Huang (PhD)
Floral and Nursery Plants Research
Research Plant Pathologist

Phone: (301) 504-9159
Fax: (301) 504-5096
Room 116

10300 BALTIMORE AVENUE
BLDG 004 BARC-WEST
BELTSVILLE, MD 207052350
Projects
Detection, Identification, and Characterization of New and Emerging Viral and Bacterial Diseases of Ornamental Plants
In-House Appropriated (D)
  Accession Number: 432744
Host Pathogen Interaction of Cool Tolerant Bacterial Wilt Strains
Non-Assistance Cooperative Agreement (S)
  Accession Number: 430955
Biology and Pathogenesis of Ralstonia solanacearum Race 3 on Geraniums and Other Hosts
Non-Assistance Cooperative Agreement (S)
  Accession Number: 431988

Publications (Clicking on the reprint icon Reprint Icon will take you to the publication reprint.)
Exploiting the real-time PCR and LAMP for development of a conjunctive new assay (FTP-LAMP) for the specific detection and differentiation of Xylella fastidiosa De Donno and mulberry strains from other subspecies/strains..... Reprint Icon - (Peer Reviewed Journal)
Elbeaino, T., Incerti, O., Dakroub, H., Valentini, F., Huang, Q. 2020. Exploiting the real-time PCR and LAMP for development of a conjunctive new assay (FTP-LAMP) for the specific detection and differentiation of Xylella fastidiosa De Donno and mulberry strains from other subspecies/strains...... Journal of Microbiological Methods. https://doi.org/10.1016/j.mimet.2020.105992.
A LuxR-type regulator, AcrR, regulates flagellar assembly and contributes to virulence, motility, biofilm formation and growth ability of Acidovorax citrulli Reprint Icon - (Peer Reviewed Journal)
Guan, W., Wang, T., Huang, Q., Tian, E., Liu, B., Yang, Y., Zhao, T. 2020. A LuxR-type regulator, AcrR, regulates flagellar assembly and contributes to virulence, motility, biofilm formation and growth ability of acidovorax citrulli. Molecular Plant Pathology. 00:1-13. https://doi.org/10.1111/mpp.12910.
Molecular and biological characterization of Ralstonia phage RsoM1USA, a new species of P2Virus, isolated in the USA Reprint Icon - (Peer Reviewed Journal)
Addy, H.S., Ebrahim, A., Huang, Q. 2019. Molecular and biological characterization of Ralstonia phage RsoM1USA, a new species of P2Virus, isolated in the USA. Frontiers in Microbiology. 10:267. https://doi.org/10.3389/fmicb.2019.00267.
Identification of a DNA region associated with the cool virulence of Ralstonia solancearum strain UW551 and its utilization for specific detection of the bacterium’s race 3 biovar 2 strains Reprint Icon - (Peer Reviewed Journal)
Stulberg, M.J., Cai, X., Ebrahim, A., Huang, Q. 2018. Identification of a DNA region associated with the cool virulence of Ralstonia solancearum strain UW551 and its utilization for specific detection of the bacterium’s race 3 biovar 2 strains. PLoS One. 13(11). https://doi.org/10.1371/journal.pone.0207280.
Host range and molecular characterization of a lytic Pradovirus-like Ralstonia phage RsPod1IDN isolated from Indonesia Reprint Icon - (Peer Reviewed Journal)
Addy, H.S., Farid, M.M., Ebrahim, A., Huang, Q. 2018. Host range and molecular characterization of a lytic Pradovirus-like Ralstonia phage RsPod1IDN isolated from Indonesia. Archives of Virology. 163:3409-3414. https://doi.org/10.1007/s00705-018-4033-1.
Sequencing, genome analysis and host range of a novel Ralstonia phage, RsoP1EGY, isolated in Egypt Reprint Icon - (Peer Reviewed Journal)
Ebrahim, A., Elhalag, K.M., Addy, H., Nasr-Eldin, M.A., Hussien, A.S., Huang, Q. 2018. Sequencing, genome analysis and host range of a novel Ralstonia phage, RsoP1EGY, isolated in Egypt. Archives of Virology. https://doi.org/10.1007/s00705-018-3844-4.
Prophage Rs551 and its repressor gene orf14 reduce virulence and increase competitive fitness of its Ralstonia solanacearum carrier strain UW551 Reprint Icon - (Peer Reviewed Journal)
Ebrahim, A., Stulberg, M.J., Huang, Q. 2017. Prophage Rs551 and its repressor gene orf14 reduce virulence and increase competitive fitness of its Ralstonia solanacearum carrier strain UW551. Frontiers in Microbiology. 8:2480. https://doi.org/10.3389/fmicb.2017.02480.
Molecular and biological characterization of 'Rs551, a filamentous bacteriophage isolated from a race 3 biovar 2 strain of Ralstonia solanacearum - (Peer Reviewed Journal)
Ebrahim, A., Stulberg, M.J., Mershon, J.P., Mollov, D.S., Huang, Q. 2017. Molecular and biological characterization of 'Rs551, a filamentous bacteriophage isolated from a race 3 biovar 2 strain of Ralstonia solanacearum. PLoS One. https://doi.org/10.1371/journal.pone.0815034.
Pectobacterium carotovorum. subsp. brasiliense is a causal agent of bacterial leaf rot of tobacco in China - (Peer Reviewed Journal)
Wang, J., Wang, Y., Dai, P., Chen, D., Zhao, T., Li, X., Huang, Q. 2017. Pectobacterium carotovorum. subsp. brasiliense is a causal agent of bacterial leaf rot of tobacco in China. Plant Disease. 101(5):830.
Detection of plant quarantine pathogen Ralstonia solanacearum r3b2 with portable POCKIT™ and BLItz® systems - (Peer Reviewed Journal)
Di, R., Huang, Q., Stulberg, M.J., Zhao, L., Levy, L. 2016. Detection of plant quarantine pathogen Ralstonia solanacearum r3b2 with portable POCKIT™ and BLItz® systems. Journal of Plant Health. 1(1):103.
Quorum-sensing contributes to virulence, twitching motility, seed attachment and biofilm formation in the wild type strain Aac-5 of Acidovorax citrulli - (Peer Reviewed Journal)
Wang, T., Guan, W., Huang, Q., Yang, Y., Yan, W., Sun, B., Zhao, T. 2016. Quorum-sensing contributes to virulence, twitching motility, seed attachment and biofilm formation in the wild type strain Aac-5 of Acidovorax citrulli. Microbial Pathogenesis. 100:133-140.
Development and comparison of TaqMan-based real-time PCR assays for detection and differentiation of Ralstonia solanacearum strains - (Peer Reviewed Journal)
Stulberg, M.J., Rascoe, J., Li, W.N., Yan, Z., Nakhla, M.K., Huang, Q. 2016. Development and comparison of TaqMan-based real-time PCR assays for detection and differentiation of Ralstonia solanacearum strains. Current Microbiology. doi:10.1007/s00284-016109-z.
A computer program for fast and easy typing of partial endoglucanase gene sequence into phylotypes and sequevars 1&2 (select agents) of Ralstonia solanacearum - (Peer Reviewed Journal)
Stalberg, M., Huang, Q. 2016. A computer program for fast and easy typing of partial endoglucanase gene sequence into phylotypes and sequevars 1&2 (select agents) of Ralstonia solanacearum. Journal of Microbiological Methods. 123:101-107.
Functional analysis of the ferric uptake requlator gene, fur, in Xanthomonas vesicatoria - (Peer Reviewed Journal)
Liu, H., Dong, C., Zhao, T., Han, J., Wang, T., Wen, X., Yang, G., Huang, Q. 2016. Functional analysis of the ferric uptake requlator gene, fur, in Xanthomonas vesicatoria. PLoS One. 11(2):e0149280. doi: 10.1371/journal.pone.0149280.
A TaqMan-based multiplex qPCR assay and DNA extraction method for phylotype IIB sequevars 1&2 (select agent) strains of Ralstonia solanacearum - (Peer Reviewed Journal)
Stulberg, M.J., Huang, Q. 2015. A TaqMan-based multiplex qPCR assay and DNA extraction method for phylotype IIB sequevars 1&2 (select agent) strains of Ralstonia solanacearum. PLoS One. doi: 10.1371/journal.pone.0139637.
Specific detection and identification of mulberry-infecting strains of Xylella fastidiosa by polymerase chain reaction - (Peer Reviewed Journal)
Guan, W., Shao, J.Y., Davis, R.E., Zhao, T., Huang, Q. 2015. Specific detection and identification of mulberry-infecting strains of Xylella fastidiosa by polymerase chain reaction. PLoS One. DOI: 10.1371/journal.pone.0129330.
A multiplex PCR assay to detect and differentiate select agent strains of Ralstonia solanacearum - (Peer Reviewed Journal)
Stulberg, M.J., Shao, J.Y., Huang, Q. 2014. A multiplex PCR assay to detect and differentiate select agent strains of Ralstonia solanacearum. Applied and Environmental Microbiology. 99:333-341.
Genome sequence of a Xylella fastidiosa strain causing sycamore leaf scorch disease in Virginia - (Peer Reviewed Journal)
Guan, W., Shao, J.Y., Davis, R.E., Zhao, T., Huang, Q. 2014. Genome sequence of a Xylella fastidiosa strain causing sycamore leaf scorch disease in Virginia. Genome Announcements. 2(4):e00773-14.
Development and systematic validation of qPCR assays for rapid and reliable differentiation of Xylella fastidiosa strains causing citrus variegated chlorosis Reprint Icon - (Peer Reviewed Journal)
Li, W., Teixeira, D.C., Hartung, J.S., Huang, Q., Chen, J., Lin, H. 2012. Development and systematic validation of qPCR assays for rapid and reliable differentiation of Xylella fastidiosa strains causing citrus variegated chlorosis. Journal of Microbiological Methods. 92:79-89.
A TaqMan-based real time PCR assay for specific detection and quantification of Xylella fastidiosa strains causing bacterial leaf scorch in oleander - (Peer Reviewed Journal)
Guan, W., Shao, J.Y., Singh, R., Davis, R.E., Huang, Q. 2012. A TaqMan-based real time PCR assay for specific detection and quantification of Xylella fastidiosa strains causing bacterial leaf scorch in oleander. Current Microbiology. 92:108-112.
Improved biovar test for Ralstonia solanacearum - (Peer Reviewed Journal)
Huang, Q., Yan, X., Wang, J. 2011. Improved biovar test for Ralstonia solanacearum. Journal of Microbiological Methods. 88:271-274.
Effect of clove oil on plant pathogenic bacteria and bacterial wilt of tomato and geranium - (Peer Reviewed Journal)
Huang, Q., Lakshman, D.K. 2011. Effect of clove oil on plant pathogenic bacteria and bacterial wilt of tomato and geranium. Journal of Plant Pathology. 92(3):701-707.
Susceptibility of Geranium Cultivars (Pelargonium spp.) to Ralstonia solanacearum - (Peer Reviewed Journal)
Norman, D.J., Huang, Q., Yuen, J.M.F., Mangravita-Novo, A., Byrne, D. 2009. Susceptibility of Geranium cultivars to Ralstonia solanacearum. HortScience. 44(5):1-5.
Specific detection and identification of Xylella fastidiosa strains causing oleander leaf scorch by polymerase chain reaction - (Abstract Only)
Huang, Qi. 2009. Specific detection and identification of Xylella fastidosa strains causing oleander leaf scorch by polymerase chain reaction. Current Microbiology. 58:393-398.
A Reliable and Inexpensive Method of Nucleic Acid Extraction for the PCR-Based Detection of Diverse Plant Pathogens - (Peer Reviewed Journal)
Li, R., Mock, R.G., Huang, Q., Abad, J., Hartung, J.S., Kinard, G.R. 2008. A Reliable and Inexpensive Method of Nucleic Acid Extraction for the PCR-Based Detection of Diverse Plant Pathogens. Journal of Virological Methods. 154:48-55.
CONSTRUCTION OF INFECTIOUS CLONES FOR DOUBLE STRANDED DNA VIRUSES OF PLANTS USING CITRUS YELLOW MOSAIC CIRUS AS AN EXAMPLE ()
Huang, Q., Hartung, J.S. 2007. Construction of infectious clones for double stranded dna viruses of plants using citrus yellow mosaic cirus as an example. Methods in Molecular Biology. 451:525-534.
NATURAL OCCURANCE OF XYLELLA FASTIDIOSA IN A COMMERCIAL NURSERY IN MARYLAND - (Peer Reviewed Journal)
Huang, Q. 2007. Natural occurances of Xyella fastidiosa in a commercial nursery in Maryland. Canadian Journal of Plant Pathology. 29:299-303.
EFFECT OF THE GROWTH REGULATOR PACLOBUTRAZOL ON GROWTH OF THE BACTERIAL PATHOGEN XYLELLA FASTIDIOSA - (Peer Reviewed Journal)
DeStefano, D.A., Grybauskas, A.P., Sherald, J.L., Momen, B., Huang, Q. and Sullivan, J.H. 2007. Effect of the growth regulator Paclobutrazol on growth of the bacterial pathogen Xylella fastidiosa. Arboriculture to Arboriculture and Urban Forestry. 33:246-252.
FAST, EASY AND EFFICIENT DNA EXTRACTION AND ONE-STEP POLYMERASE CHAIN REACTION FOR THE DETECTION OF XYLELLA FASTIDIOSA IN POTENTIAL INSECT VECTORS - (Peer Reviewed Journal)
Huang, Q., Bentz, J. and Sherald, J.L. 2006. Fast, easy and effecient DNA extraction and one-step PCR for the detection of Xyllela fastidiosa in potential insect vectors. 88:77-81.
IDENTIFICATION OF OPEN READNG FRAMES UNIQUE TO A SELECT AGENT: RALSTONIA SOLANACEARUM RACE 3 BIOVAR 2 - (Peer Reviewed Journal)
Gabriel, D.W., Allen, C.A., Schell, M., Denny, T., Greenberg, J., Duan, Y.P., Flores-Cruz, Z., Huang, Q., Clifford, J.M., Presting, G., Gonzelez, E.T., Reedy, J., Elphinstone, J., Swanson, J., Yao, J., Mulholand, V., Liu, L., Farmerie, W., Patnaikuni, M., Galogh, B., Norman, D., Alverez, A., Castillo, J., Saddler, G., Walunas, T., Zhukov, A. and Mikhailova, N. 2006. Molecular Plant-Microbe Interaction. Identification of open reading frames unique to a select agent: Ralstonia solanacearum race 3, biovar 2. 19:69-79.
TRANSGENIC APPROACHES TO DISEASE RESISTANCE IN ORNAMENTAL CROPS - (Peer Reviewed Journal)
Hammond, J., Hsu, H.T., Huang, Q., Jordan, R.L., Kamo, K.K., Pooler, M.R. 2006. Transgenic approaches to disease resistance in ornamental crops. Journal of Crop Improvement. 17:155-210.
FIRST REPORT OF OLEANDER LEAF SCORCH CAUSED BY XYLELLA FASTIDIOSA IN TEXAS - (Peer Reviewed Journal)
Huang, Q., Brlansky, R.H., Barnes, L., Li, W.N., Hartung, J.S. 2004. First report of Oleander leaf scorch caused by Xylella fastidiosa in Texas. Plant Disease. 88:1049.
FIRST REPORT OF XYLELLA FASTIDIOSA IN BLACK OAK IN WASHINGTON, DC - (Peer Reviewed Journal)
Huang, Q. First Report of Xylella Fastidiosa Associated with Leaf Scorch in Black Oak in Washington, DC. Journal of Plant Disease. v:88, pp:224.
FIRST REPORT OF XYLELLA FERTIDIOSA IN JAPANESE BEECH BONSAI - (Peer Reviewed Journal)
Huang, Q., Li, W.N., Hartung, J.S. 2003. First report of xylella fertidiosa in japanese beech bonsai. Canadian Journal of Plant Pathology. 25:401-405.
ISOLATION AND PHYLOGENETIC ANALYSIS OF XYLELLA FASTIDIOSA FROM ITS INVASIVE ALTERNATIVE HOST, PORCELAIN BERRY - (Peer Reviewed Journal)
Huang, Q., Sherald, J. 2004. Isolation and phylogenetic analysis of xylella fastidiosa from its invasive alternative host, porcelain berry. Current Microbiology. 48:73-76.
IDENTIFICATION OF A PROMOTER REGION FROM CITRUS YELLOW MOSAIC VIRUS ()
ISOLATION OF AN EFFECTIVE PROMOTER FROM CITRUS YELLOW MOSAIC VIRUS FOR EXPRESSION OF DISEASE RESISTANT GENES IN ORNAMENTAL CROPS ()
ULTRASTRUCTURE OF CITRUS INFECTED WITH CITRUS YELLOW MOSAIC - (Abstract Only)
Brlansky, R.H., Howd, D.S., Huang, Q., Hartung, J.S. 2002. Ultrastructure of citrus infected with citrus yellow mosaic. Conference of International Organization of Citrus Virologists.
CLONING AND SEQUENCE ANALYSIS OF A CITRUS YELLOW MOSAIC VIRUS GENOME INFECTIOUS TO SWEET ORANGE VIA AGROBACTERIUM-MEDIATED INOCULATION - (Peer Reviewed Journal)
HOST RANGE AND PCR DETECTION OF CITRUS YELLOW MOSAIC VIRUS - (Abstract Only)
Huang, Q., Hartung, J.S. 2001. Host range and PCR detection of citrus yellow mosaic virus. Phytopathology.
A CLONED CITRUS YELLOW MOSAIC VIRUS GENOME IS INFECTIOUS IN SWEET ORANGE - (Abstract Only)
Huang, Q., Hartung, J.S. 2001. A cloned citrus yellow mosaic virus genome is infectious in sweet orange. BARC Poster Day.
MOLECULAR CHARACTERIZATION OF CITRUS YELLOW MOSAIC VIRUS - (Abstract Only)
MOLECULAR CHARACTERIZATION OF THE CITRUS YELLOW MOSAIC VIRUS - (Abstract Only)
Huang, Q., Hartung, J.S. 2000. Molecular characterization of the citrus yellow mosaic virus. BARC Poster Day.
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