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ARS Home » Northeast Area » Washington, D.C. » National Arboretum » Floral and Nursery Plants Research » People & Locations » Qi Huang
Qi Huang
Floral and Nursery Plants Research
Research Plant Pathologist

Phone: (301) 504-9159
Fax: (301) 504-5096
Room 116

10300 BALTIMORE AVENUE
BLDG 004 BARC-WEST
BELTSVILLE , MD 207052350
Projects
Detection, Identification, and Characterization of New and Emerging Viral and Bacterial Diseases of Ornamental Plants
In-House Appropriated (D)
  Accession Number: 432744
Specific Detection of Xylella Fastidiosa Subspecies Pauca-CoDiRO Strain Using Molecular Approaches
Interagency Reimbursable Agreement (I)
  Accession Number: 432862
Host Pathogen Interaction of Cool Tolerant Bacterial Wilt Strains
Non-Assistance Cooperative Agreement (S)
  Accession Number: 430955
Biology and Pathogenesis of Ralstonia solanacearum Race 3 on Geraniums and Other Hosts
Non-Assistance Cooperative Agreement (S)
  Accession Number: 431988

Publications (Clicking on the reprint icon Reprint Icon will take you to the publication reprint.)
Sequencing, genome analysis and host range of a novel Ralstonia phage RsoP1EGY isolated from Egypt -
Ebrahim, A., Elhalag, K.M., Addy, H., Hussien, A.S., Nasr-Eldin, M.A., Huang, Q. 2018. Sequencing, genome analysis and host range of a novel Ralstonia phage RsoP1EGY isolated from Egypt. Archives of Virology. https://doi.org/10.1007/s00705-018-3844-4.
Prophage Rs551 and its repressor gene orf14 reduce virulence and increase competitive fitness of its Ralstonia solanacearum carrier strain UW551 -
Ebrahim, A., Stulberg, M.J., Huang, Q. 2017. Prophage Rs551 and its repressor gene orf14 reduce virulence and increase competitive fitness of its Ralstonia solanacearum carrier strain UW551. Frontiers in Microbiology. 8:2480. https://doi.org/10.3389/fmicb.2017.02480.
Molecular and biological characterization of 'Rs551, a filamentous bacteriophage isolated from a race 3 biovar 2 strain of Ralstonia solanacearum -
Ebrahim, A., Stulberg, M.J., Mershon, J.P., Mollov, D.S., Huang, Q. 2017. Molecular and biological characterization of 'Rs551, a filamentous bacteriophage isolated from a race 3 biovar 2 strain of Ralstonia solanacearum. PLoS One. https://doi.org/10.1371/journal.pone.0815034.
Pectobacterium carotovorum. subsp. brasiliense is a causal agent of bacterial leaf rot of tobacco in China -
Wang, J., Wang, Y., Dai, P., Chen, D., Zhao, T., Li, X., Huang, Q. 2017. Pectobacterium carotovorum. subsp. brasiliense is a causal agent of bacterial leaf rot of tobacco in China. Plant Disease. 101(5):830.
Detection of plant quarantine pathogen Ralstonia solanacearum r3b2 with portable POCKIT™ and BLItz® systems -
Di, R., Huang, Q., Stulberg, M.J., Zhao, L., Levy, L. 2016. Detection of plant quarantine pathogen Ralstonia solanacearum r3b2 with portable POCKIT™ and BLItz® systems. Journal of Plant Health. 1(1):103.
Quorum-sensing contributes to virulence, twitching motility, seed attachment and biofilm formation in the wild type strain Aac-5 of Acidovorax citrulli -
Wang, T., Guan, W., Huang, Q., Yang, Y., Yan, W., Sun, B., Zhao, T. 2016. Quorum-sensing contributes to virulence, twitching motility, seed attachment and biofilm formation in the wild type strain Aac-5 of Acidovorax citrulli. Microbial Pathogenesis. 100:133-140.
Development and comparison of TaqMan-based real-time PCR assays for detection and differentiation of Ralstonia solanacearum strains -
Stulberg, M.J., Rascoe, J., Li, W.N., Yan, Z., Nakhla, M.K., Huang, Q. 2016. Development and comparison of TaqMan-based real-time PCR assays for detection and differentiation of Ralstonia solanacearum strains. Current Microbiology. doi:10.1007/s00284-016109-z.
A computer program for fast and easy typing of partial endoglucanase gene sequence into phylotypes and sequevars 1&2 (select agents) of Ralstonia solanacearum -
Stalberg, M., Huang, Q. 2016. A computer program for fast and easy typing of partial endoglucanase gene sequence into phylotypes and sequevars 1&2 (select agents) of Ralstonia solanacearum. Journal of Microbiological Methods. 123:101-107.
Functional analysis of the ferric uptake requlator gene, fur, in Xanthomonas vesicatoria -
Liu, H., Dong, C., Zhao, T., Han, J., Wang, T., Wen, X., Yang, G., Huang, Q. 2016. Functional analysis of the ferric uptake requlator gene, fur, in Xanthomonas vesicatoria. PLoS One. 11(2):e0149280. doi: 10.1371/journal.pone.0149280.
A TaqMan-based multiplex qPCR assay and DNA extraction method for phylotype IIB sequevars 1&2 (select agent) strains of Ralstonia solanacearum -
Stulberg, M.J., Huang, Q. 2015. A TaqMan-based multiplex qPCR assay and DNA extraction method for phylotype IIB sequevars 1&2 (select agent) strains of Ralstonia solanacearum. PLoS One. doi: 10.1371/journal.pone.0139637.
Specific detection and identification of mulberry-infecting strains of Xylella fastidiosa by polymerase chain reaction -
Guan, W., Shao, J.Y., Davis, R.E., Zhao, T., Huang, Q. 2015. Specific detection and identification of mulberry-infecting strains of Xylella fastidiosa by polymerase chain reaction. PLoS One. DOI: 10.1371/journal.pone.0129330.
A multiplex PCR assay to detect and differentiate select agent strains of Ralstonia solanacearum -
Stulberg, M.J., Shao, J.Y., Huang, Q. 2014. A multiplex PCR assay to detect and differentiate select agent strains of Ralstonia solanacearum. Applied and Environmental Microbiology. 99:333-341.
Genome sequence of a Xylella fastidiosa strain causing sycamore leaf scorch disease in Virginia -
Guan, W., Shao, J.Y., Davis, R.E., Zhao, T., Huang, Q. 2014. Genome sequence of a Xylella fastidiosa strain causing sycamore leaf scorch disease in Virginia. Genome Announcements. 2(4):e00773-14.
Development and systematic validation of qPCR assays for rapid and reliable differentiation of Xylella fastidiosa strains causing citrus variegated chlorosis Reprint Icon -
Li, W., Teixeira, D.C., Hartung, J.S., Huang, Q., Chen, J., Lin, H. 2012. Development and systematic validation of qPCR assays for rapid and reliable differentiation of Xylella fastidiosa strains causing citrus variegated chlorosis. Journal of Microbiological Methods. 92:79-89.
A TaqMan-based real time PCR assay for specific detection and quantification of Xylella fastidiosa strains causing bacterial leaf scorch in oleander -
Guan, W., Shao, J.Y., Singh, R., Davis, R.E., Huang, Q. 2012. A TaqMan-based real time PCR assay for specific detection and quantification of Xylella fastidiosa strains causing bacterial leaf scorch in oleander. Current Microbiology. 92:108-112.
Improved biovar test for Ralstonia solanacearum -
Huang, Q., Yan, X., Wang, J. 2011. Improved biovar test for Ralstonia solanacearum. Journal of Microbiological Methods. 88:271-274.
Effect of clove oil on plant pathogenic bacteria and bacterial wilt of tomato and geranium -
Huang, Q., Lakshman, D.K. 2011. Effect of clove oil on plant pathogenic bacteria and bacterial wilt of tomato and geranium. Journal of Plant Pathology. 92(3):701-707.
Susceptibility of Geranium Cultivars (Pelargonium spp.) to Ralstonia solanacearum -
Norman, D.J., Huang, Q., Yuen, J.M.F., Mangravita-Novo, A., Byrne, D. 2009. Susceptibility of Geranium cultivars to Ralstonia solanacearum. HortScience. 44(5):1-5.
Specific detection and identification of Xylella fastidiosa strains causing oleander leaf scorch by polymerase chain reaction -
Huang, Qi. 2009. Specific detection and identification of Xylella fastidosa strains causing oleander leaf scorch by polymerase chain reaction. Current Microbiology. 58:393-398.
A Reliable and Inexpensive Method of Nucleic Acid Extraction for the PCR-Based Detection of Diverse Plant Pathogens -
Li, R., Mock, R.G., Huang, Q., Abad, J., Hartung, J.S., Kinard, G.R. 2008. A Reliable and Inexpensive Method of Nucleic Acid Extraction for the PCR-Based Detection of Diverse Plant Pathogens. Journal of Virological Methods. 154:48-55.
CONSTRUCTION OF INFECTIOUS CLONES FOR DOUBLE STRANDED DNA VIRUSES OF PLANTS USING CITRUS YELLOW MOSAIC CIRUS AS AN EXAMPLE -
Huang, Q., Hartung, J.S. 2007. Construction of infectious clones for double stranded dna viruses of plants using citrus yellow mosaic cirus as an example. Methods in Molecular Biology. 451:525-534.
NATURAL OCCURANCE OF XYLELLA FASTIDIOSA IN A COMMERCIAL NURSERY IN MARYLAND -
Huang, Q. 2007. Natural occurances of Xyella fastidiosa in a commercial nursery in Maryland. Canadian Journal of Plant Pathology. 29:299-303.
EFFECT OF THE GROWTH REGULATOR PACLOBUTRAZOL ON GROWTH OF THE BACTERIAL PATHOGEN XYLELLA FASTIDIOSA -
DeStefano, D.A., Grybauskas, A.P., Sherald, J.L., Momen, B., Huang, Q. and Sullivan, J.H. 2007. Effect of the growth regulator Paclobutrazol on growth of the bacterial pathogen Xylella fastidiosa. Arboriculture to Arboriculture and Urban Forestry. 33:246-252.
FAST, EASY AND EFFICIENT DNA EXTRACTION AND ONE-STEP POLYMERASE CHAIN REACTION FOR THE DETECTION OF XYLELLA FASTIDIOSA IN POTENTIAL INSECT VECTORS -
Huang, Q., Bentz, J. and Sherald, J.L. 2006. Fast, easy and effecient DNA extraction and one-step PCR for the detection of Xyllela fastidiosa in potential insect vectors. 88:77-81.
IDENTIFICATION OF OPEN READNG FRAMES UNIQUE TO A SELECT AGENT: RALSTONIA SOLANACEARUM RACE 3 BIOVAR 2 -
Gabriel, D.W., Allen, C.A., Schell, M., Denny, T., Greenberg, J., Duan, Y.P., Flores-Cruz, Z., Huang, Q., Clifford, J.M., Presting, G., Gonzelez, E.T., Reedy, J., Elphinstone, J., Swanson, J., Yao, J., Mulholand, V., Liu, L., Farmerie, W., Patnaikuni, M., Galogh, B., Norman, D., Alverez, A., Castillo, J., Saddler, G., Walunas, T., Zhukov, A. and Mikhailova, N. 2006. Molecular Plant-Microbe Interaction. Identification of open reading frames unique to a select agent: Ralstonia solanacearum race 3, biovar 2. 19:69-79.
TRANSGENIC APPROACHES TO DISEASE RESISTANCE IN ORNAMENTAL CROPS -
Hammond, J., Hsu, H.T., Huang, Q., Jordan, R.L., Kamo, K.K., Pooler, M.R. 2006. Transgenic approaches to disease resistance in ornamental crops. Journal of Crop Improvement. 17:155-210.
FIRST REPORT OF OLEANDER LEAF SCORCH CAUSED BY XYLELLA FASTIDIOSA IN TEXAS -
Huang, Q., Brlansky, R.H., Barnes, L., Li, W.N., Hartung, J.S. 2004. First report of Oleander leaf scorch caused by Xylella fastidiosa in Texas. Plant Disease. 88:1049.
FIRST REPORT OF XYLELLA FASTIDIOSA IN BLACK OAK IN WASHINGTON, DC -
Huang, Q. First Report of Xylella Fastidiosa Associated with Leaf Scorch in Black Oak in Washington, DC. Journal of Plant Disease. v:88, pp:224.
FIRST REPORT OF XYLELLA FERTIDIOSA IN JAPANESE BEECH BONSAI -
Huang, Q., Li, W.N., Hartung, J.S. 2003. First report of xylella fertidiosa in japanese beech bonsai. Canadian Journal of Plant Pathology. 25:401-405.
ISOLATION AND PHYLOGENETIC ANALYSIS OF XYLELLA FASTIDIOSA FROM ITS INVASIVE ALTERNATIVE HOST, PORCELAIN BERRY -
Huang, Q., Sherald, J. 2004. Isolation and phylogenetic analysis of xylella fastidiosa from its invasive alternative host, porcelain berry. Current Microbiology. 48:73-76.
IDENTIFICATION OF A PROMOTER REGION FROM CITRUS YELLOW MOSAIC VIRUS -
ISOLATION OF AN EFFECTIVE PROMOTER FROM CITRUS YELLOW MOSAIC VIRUS FOR EXPRESSION OF DISEASE RESISTANT GENES IN ORNAMENTAL CROPS -
ULTRASTRUCTURE OF CITRUS INFECTED WITH CITRUS YELLOW MOSAIC -
Brlansky, R.H., Howd, D.S., Huang, Q., Hartung, J.S. 2002. Ultrastructure of citrus infected with citrus yellow mosaic. Conference of International Organization of Citrus Virologists.
CLONING AND SEQUENCE ANALYSIS OF A CITRUS YELLOW MOSAIC VIRUS GENOME INFECTIOUS TO SWEET ORANGE VIA AGROBACTERIUM-MEDIATED INOCULATION -
HOST RANGE AND PCR DETECTION OF CITRUS YELLOW MOSAIC VIRUS -
Huang, Q., Hartung, J.S. 2001. Host range and PCR detection of citrus yellow mosaic virus. Phytopathology.
A CLONED CITRUS YELLOW MOSAIC VIRUS GENOME IS INFECTIOUS IN SWEET ORANGE -
Huang, Q., Hartung, J.S. 2001. A cloned citrus yellow mosaic virus genome is infectious in sweet orange. BARC Poster Day.
MOLECULAR CHARACTERIZATION OF CITRUS YELLOW MOSAIC VIRUS -
MOLECULAR CHARACTERIZATION OF THE CITRUS YELLOW MOSAIC VIRUS -
Huang, Q., Hartung, J.S. 2000. Molecular characterization of the citrus yellow mosaic virus. BARC Poster Day.
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