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United States Department of Agriculture

Agricultural Research Service

Related Topics

Dominic Wong
Bioproducts Research
Chemistry

Phone: (510) 559-5860
Fax: (510) 559-5818
Room 3117

USDA, ARS, PWA, WRRC-BCE
800 BUCHANAN STREET
ALBANY , CA 94710

Projects
Technologies for Improving Industrial Biorefineries that Produce Marketable Biobased Products
Appropriated (D)
  Accession Number: 427427

Publications (Clicking on the reprint icon Reprint Icon will take you to the publication reprint.)
Cloning and expression of Pectobacterium carotovorum endo-polygalacturonase gene in Pichia pastoris for production of oligogalacturonates -
Wong, D., Rafique, N., Tabassum, R., Awan, S., Orts, W.J. 2016. Cloning and expression of Pectobacterium carotovorum endo-polygalacturonase gene in Pichia pastoris for production of oligogalacturonates. BioResources. 11(2):5204-5214.
Glucuronoyl esterases are active on polymeric substrate, methyl esterified glucuronoxylan -
Biely, P., Malovikova, A., Uhliarikova, I., Li, X., Wong, D. 2015. Glucuronoyl esterases are active on polymeric substrate, methyl esterified glucuronoxylan. FEBS Letters. 589:2334-2339.
From metagenomic gene discovery to enzymatic breakdown of crosslinks in agricultural fibers for functional products -
Wong, D., Chan, V.J., Marakami, M. 2015. From metagenomic gene discovery to enzymatic breakdown of crosslinks in agricultural fibers for functional products. Pacifichem Symposium. Meeting Abstract, BIOL 343.
A novel feruloyl esterase from rumen microbial metagenome: Gene cloning and enzyme characterization in the release of mono- and diferulic acids -
Wong, D., Takeoka, G.R., Chan, V.J., Liao, H., Marakami, M. 2015. A novel feruloyl esterase from rumen microbial metagenome: Gene cloning and enzyme characterization in the release of mono- and diferulic acids. Protein and Peptide Letters. 22(2):681-688.
Structural basis for xyloglucan specificity within GH5 family and the molecular determinants for a-D-Xylp(1¿6)-D-Glcp recognition at the -1 subsite -
Santos, C.R., Cordeiro, R.L., Wong, D., Murakami, M.T. 2015. Structural basis for xyloglucan specificity within GH5 family and the molecular determinants for a-D-Xylp(1¿6)-D-Glcp recognition at the -1 subsite. Biochemistry. 54:1930-1942.
Engineering yeast for the expression and secretion of cellulase cocktails -
Cloning of a novel feruloyl esterase gene from rumen microbial metagenome and enzyme characterization in synergism with endoxylanases -
Wong, D., Chan, V.J., Liao, H., Zidwick, M. 2013. Cloning of a novel feruloyl esterase gene from rumen microbial metagenome and enzyme characterization in synergism with endoxylanases. Journal of Industrial Microbiology. 40:287-295.
Xylanase XYN IV from Trichoderma reesei showing exo- and endo-xylanase activity -
Wong, D., Tenkanen, M., Vrsanaka, M., Sika-Aho, M., Puchart, V., Penttila, M., Salohelmo, M., Biely, P. 2012. Xylanase XYN IV from Trichoderma reesei showing exo- and endo-xylanase activity. FEBS Journal. 280: 295-301.
Engineering Saccharomyces cerevisiae for consolidated bioprocessing in starch and biomass conversion -
Wong, D., Batt Throne, S.B., Chan, V.J. 2011. Engineering Saccharomyces cerevisiae for consolidated bioprocessing in starch and biomass conversion. [Abstract]. Society of Microbiology and Biotechnology 2011 Annual Meeting, July 24-28,2011 New Orleans, Louisana.
Engineering Saccharomyces cerevisiae to produce feruloyl esterase for the release of ferulic acid from switchgrass -
Wong, D., Chan, V.J., Batt Throne, S.B., Sarath, G., Liao, H. 2011. Engineering Saccharomyces cerevisiae to produce feruloyl esterase for the release of ferulic acid from switchgrass. Journal of Industrial Microbiology and Biotechnology. Epub ahead of print. DOI: 10.1007/s10295-011-0985-9.
Functional characterization of two distinct xyoglucanases from rumenal microbes -
Wong, D., Chan, V.J., Mccormack, A.A., Batt Throne, S.B. Functional characterization of two distinct xyoglucanases from rumenal microbes. Meeting Abstract, AGFD 125.
Cloning and characterization of an exo-xylglucanase from rumenal microbial metagenome -
Wong, D., Chan, V.J., Mccormack, A.A., Batt Throne, S.B. 2010. Cloning and characterization of an exo-xylglucanase from rumenal microbial metagenome. Protein and Peptide Letters. 17:803-808.
Expression and secretion of cellulosmal enzymes by engineered Saccharomyces cerevisiae -
Batt Throne, S.B., Wan, D., Doi, R.H., Wong, D. 2010. Expression and secretion of cellulosmal enzymes by engineered Saccharomyces cerevisiae. Meeting Abstract, 2010; CELL Poster Session; Poster #57 (Paper ID 18330).
Cloning and characterization of alpha-glucuronidase enzyme -
Lee, C.C., Kibblewhite, R.E., Wagschal, K.C., Wong, D., Orts, W.J. 2010. Cloning and characterization of alpha-glucuronidase enzyme. Meeting Abstract.
Cloning and characterization of alpha-glucuronidase enzyme -
Lee, C.C., Kibblewhite, R.E., Wagschal, K.C., Wong, D., Orts, W.J. 2010. Cloning and characterization of alpha-glucuronidase enzyme. Meeting Abstract.
Chromosomal integration of recombinant alpha-amylase and glucoamylase genes in saccharomyces cerevisiae for starch conversion -
Wong, D., Batt Throne, S.B., Robertson, G.H., Lee, C.C., Wagschal, K.C. 2010. Chromosomal integration of recombinant alpha-amylase and glucoamylase genes in saccharomyces cerevisiae for starch conversion. Industrial Biotechnology. 6:112-118.
A novel xyloglucan-specific endo-beta-1,4-glucanase: biochemical properties and inhibition studies -
Wong, D., Chan, V.J., Mccormack, A.A., Batt Throne, S.B. 2010. A novel xyloglucan-specific endo-beta-1,4-glucanase: biochemical properties and inhibition studies. Applied Microbiology and Biotechnology. 86:1463-1471.
Engineer Sccharomyces cerevisiae for consolidated bioprocessing -
Wong, D., Batt Throne, S.B., Wan, D., Lee, C.C., Wagschal, K.C. 2009. Engineer Sccharomyces cerevisiae for consolidated bioprocessing. Meeting Abstract.
Applications of metagenomics for industrial bioproducts -
Wong, D. 2010. Applications of metagenomics for industrial bioproducts. Wong, D. Applications of metagenomics for industrial bioproducts. In: Marco, D., editor. Metagenomics: Theory, methods and applications. Norfolk, UK. Caister Academic Press.p. 141-158.
Cloning biomass-degrading enzymes from environmental sources -
Lee, C.C., Wong, D., Wagschal, K.C., Orts, W.J. 2009. Cloning biomass-degrading enzymes from environmental sources. Meeting Abstract.
Functional cloning of an endo-alpha-1,5-L-arabinanase gene from a metagenomic library -
Wong, D., Chan, V.J., Mccormack, A.A. 2009. Functional cloning of an endo-alpha-1,5-L-arabinanase gene from a metagenomic library. Protein and Peptide Letters. 16:1411-1435.
Purification and characterization of a glycoside hydrolase family 43 Beta-xylosidase from Geobacillus thermoleovorans IT-08 -
Wagschal, K.C., Heng, C., Lee, C.C., Robertson, G.H., Orts, W.J., Wong, D. 2008. Purification and characterization of a glycoside hydrolase family 43 Beta-xylosidase from Geobacillus thermoleovorans IT-08. Applied Biochemistry and Biotechnology. 155(1-3):1-10.
Biochemical characterization of a novel dual-function a-L-arabinofuranosidase/b-xylosidase isolated from a compost starter mixture -
Wagschal, K.C., Heng, C., Lee, C.C., Wong, D. 2009. Biochemical characterization of a novel dual-function a-L-arabinofuranosidase/b-xylosidase isolated from a compost starter mixture. Applied Microbiology and Biotechnology. 81:855-863.
Metagenomic gene discovery and enzyme engineering for biomass conversion -
Wong, D., Lee, C.C., Wagschal, K.C., Smith, M.R., Robertson, G.H., Orts, W.J. Metagenomic gene discovery and enzyme engineering for biomass conversion. American Chemical Society Western Regional Meeting. American Chemical Society Symposium: New Developments in Biofuels Research. Abstract 204.
Gene discovery and engineering for biomass degradation -
Lee, C.C., Wagschal, K.C., Wong, D. 2008. Gene discovery and engineering for biomass degradation. United States-Japan Cooperative Program in Natural Resources. 2008. 50-51.
Structure and Action Mechanism of Ligninolytic Enzymes -
Wong, D. 2009. Structure and Action Mechanism of Ligninolytic Enzymes. Applied Biochemistry and Biotechnology. 157(2):174-209.
Cloning of Bacillus licheniformis xylanase gene and characterization of recombinant enzyme -
Lee, C.C., Accinelli, R., Smith, M.R., Wagschal, K.C., Orts, W.J., Wong, D. 2008. Cloning of Bacillus licheniformis xylanase gene and characterization of recombinant enzyme. Current Microbiology. 57:301-305.
Biofuels Group NSF DUSEL Project -
Bang, S., Sani, R., Bleakly, B., Bischoff, K.M., Hughes, S.R., Wagschal, K.C., Wong, D., Lee, C.C., Jordan, D.B., Pokkuluri, R., Schiffer, M. 2008. Biofuels Group NSF DUSEL Project [abstract]. Homestake DUSEL Spring Workshop. Talk 10. p. 2.
Cloning, Expression and Characterization of a Glycoside Hydrolase Family 39 Xylosidase from Bacillus Halodurans C-125 -
Wagschal, K.C., Franquivillanueva, D.M., Lee, C.C., Robertson, G.H., Wong, D. Cloning, Expression and Characterization of a Glycoside Hydrolase Family 39 Xylosidase from Bacillus Halodurans C-125. Applied Biochemistry and Biotechnology.
Cloning and characterization of xylanase gene from hot spring microorganism -
Lee, C.C., Accinelli, R., Smith, M.R., Wagschal, K.C., Orts, W.J., Wong, D. Cloning and characterization of xylanase gene from hot spring microorganism. Meeting Abstract #307.
Enzymatic Deconstruction of Backbone Structures of the Ramified Regions in Pectins -
Wong, D. 2007. Enzymatic Deconstruction of Backbone Structures of the Ramified Regions in Pectins. Protein Journal. 27(1):30-42.
Developing enzyme systems for biomass deconstruction -
Wong, D., Lee, C.C., Wagschal, K.C., Smith, M.R., Robertson, G.H., Orts, W.J. 2007. Developing enzyme systems for biomass deconstruction. Meeting Abstract. Paper No. 217
SYNERGISTIC ACTION OF ALPHA-AMYLASE AND GLUCOAMYLASE ON THE HYDROLYSIS OF NATIVE STARCH GRANULES -
Wong, D., Robertson, G.H., Batt, S.B., Lee, C.C., Wagschal, K.C. 2007. Synergistic action of alpha-amylase and glucoamylase on the hydrolysis of native starch granules. Protein and Peptide Letters. 26:159-164.
EXPRESSION AND SECRETION OF CLOSTRIDIUM CELLULOVORANS XYNA IN SACCHAROMYCES CEREVISIAE -
Batt, S.B., Wong, D., Wagschal, K.C., Lee, C.C. 2006. Expression and secretion of clostridium cellulovorans xyna in saccharomyces cerevisiae. Division of Biological Chemistry, Enzymes Program. Abstract 220.
GENETIC AND BIOCHEMICAL CHARACTERIZATION OF A NOVEL BACTERIAL A-L-ARABINOFURANOSIDASE -
Wagschal, K.C., Franqui Espiet, D., Lee, C.C., Accinelli, R., Robertson, G.H., Wong, D. 2007. Genetic and biochemical characterization of a novel bacterial a-l-arabinofuranosidase. Enzyme and Microbial Technology. (40):747-753.
EVOLUTIONARY ENZYME DESIGN FOR IMPROVED BIOREFINING OF CROPS AND RESIDUES -
Wong, D., Lee, C.C., Wagschal, K.C., Smith, M.R., Robertson, G.H., Orts, W.J. 2006. Evolutionary enzyme design for improved biorefining of crops and residues. Technical Program (abstract). P8.
SYNERGISTIC ACTION OF BARLEY ALPHA-AMYLASE AND LENTINULA EDODES GLUCOAMYLASE ON RAW STARCH HYDROLYSIS -
Wong, D., Robertson, G.H., Lee, C.C., Wagschal, K.C. 2006. Synergistic action of barley alpha-amylase and lentinula edodes glucoamylase on raw starch hydrolysis. Approved 10/27/2005. American Society of Biochemistry & Molecular Biology, San Francisco, CA. April 1-4, 2006, Program 73.16, vol A27.
FERULOYL ESTERASE - A KEY ENZYME IN BIOMASS DEGRADATION -
Wong, D. Feruloyl esterase - a key enzyme in biomass degradation. 2005. Applied Biochemistry and Biotechnology, 133:87-112.
CLONING AND CHARACTERIZATION OF COLD-ACTIVE XYLANASE ENZYMES -
Lee, C.C., Accinelli, R., Wagschal, K.C., Robertson, G.H., Wong, D. 2006. Cloning and characterization of cold-active xylanase enzymes. 28th Symposium on Biotechnology for Fuels and Chemicals. Paper No. 1A-41.
CLONING AND CHARACTERIZATION OF A COLD ACTIVE XYLANASE ENZYME -
Lee, C.C., Accinelli, R., Wagschal, K.C., Robertson, G.H., Wong, D. 2006. Cloning and characterization of a cold active xylanase enzyme. Extremophiles. 10:295-300.
NATIVE OR RAW STARCH DIGESTION -
Robertson, G.H., Wong, D., Lee, C.C., Wagschal, K.C., Smith, M.R., Orts, W.J. 2006. Native or raw starch digestion: A Key step in energy efficient biorefining of grain. Journal of Agricultural and Food Chemistry, 54:353-65.
FERULOYL ESTERASE - A KEY ENZYME IN BIOMASS DEGRADATION -
Wong, D. 2005. Feruloyl esterase - a key enzyme in biomass degradation. Applied Biochemistry and Biotechnology. 133:87-112.
ISOLATION AND CHARACTERIZATION OF A PSYCHROPHILIC XYLANASE ENZYME FROM FLAVOBACTERIUM SP. -
Lee, C.C., Smith, M.R., Accinelli, R., Williams, T.G., Wagschal, K.C., Wong, D., Robertson, G.H. 2006. Isolation and characterization of a psychrophilic xylanase enzyme from flavobacterium sp. Current Microbiology, 52(2):112-116.
CHARACTERIZATION OF ACTIVE LENTINULA EDODES GLUCOAMYLASE EXPRESSED AND SECRETED BY SACCHAROMYCES CEREVISIAE -
Wong, D., Batt, S.B., Lee, C.C., Wagschal, K.C., Robertson, G.H. 2005. Characterization of active lentinula edodes glucoamylase expressed and secreted by saccharomyces cerevisiae. Protein Journal. vol 24, pp 455-463.
CHARACTERIZATION AND DIRECTED EVOLUTION OF HEMICELLULOTIC ENZYMES USING NATURAL XYLOOLIGOACCHARIDE SUBSTRATES -
Wagschal, K.C., Franqui Espiet, D., Lee, C.C., Robertson, G.H., Wong, D.W.S. 2005. Characterization of hemicellulotic degrading enzymes using natural substrates. Symposium on Biotechnology for Fuels and Chemicals, Denver, CO. Paper No. 1B-65. Abstract A10, Enzyme Engineering XVIII.
ENZYME-COUPLED ASSAY FOR BETA-XYLOSIDASE HYDROLYSIS OF NATURAL SUBSTRATES -
Wagschal, K.C., Franqui Espiet, D., Lee, C.C., Robertson, G.H., Wong, D. 2005. Enzyme-coupled assay for beta-xylosidase hydrolysis of natural substrates. Applied and Environmental Microbiology. 71:5318-5323.
EVOLVED ENZYMES FOR COLD OR NON-COOKING HYDROLYSIS OF STARCHES TO FERMENTATION FEEDSTOCK -
Robertson, G.H., Wong, D., Lee, C.C., Wagschal, K.C., Smith, M.R. 2005. Evolved enzymes for cold or non-cooking hydrolysis of starches to fermentation feedstock. In Proceedings of the North American Grain Congress, February 20-22, 2005, Reno, NV. 2005 CDROM.
EXPRESSION AND CHARACTERIZATION OF ACTIVE LENTINUS EDODES GLUCOAMYLASE IN SACCHAROMYCES CEREVISIAE -
Wong, D., Batt, S.B., Lee, C.C., Wagschal, K.C., Robertson, G.H. 2005. Expression and characterization of active. lentinus edodes glucoamylase in saccharomyces cerevisiae. Presentation 04:05. Paper No 11277.
CLONING AND CHARACTERIZATION OF BIOMASS DEGRADING ENZYMES -
Lee, C.C., Accinelli, R., Batt, S.B., Williams, T.G., Wong, D., Wagschal, K.C., Robertson, G.H. 2005. Cloning and characterization of biomass degrading enzymes. Symposium on Biotechnology for Fuels and Chemicals, Denver, CO. Paper No. IB-41 (Abstract).
RAW STARCH DIGESTION OR NON-COOKING BREAKDOWN OF STARCH PRIOR TO FERMENTATION -
Robertson, G.H., Wong, D. 2005. Raw starch digestion or non-cooking breakdown of starch prior to fermentation. Fuel Ethanol Workshop and Trade Show, June 28, 2005, Kansas City, MO. Paper No.8:1.
DIRECTED EVOLUTION OF BARLEY ALPHA-AMYLASE FOR INCREASED ACTIVITY -
Batt, S.B., Wong, D., Lee, C.C., Wagschal, K.C., Robertson, G.H. 2004. Directed evolution of barley alpha-amylase for increased activity. American Chemical Society Western Regional Meeting, Sacramento, CA. Paper No. 144 (Abstracts).
ENVISIONING BIOREFINERIES BASED ON UTILIZATION OF LIGNOCELLULOSIC STRAWS AND BAGASSESS -
Orts, W.J., Imam, S.H., Glenn, G.M., Wong, D., Inglesby, M.K., Guttman, M.E., Samac, D.A. 2004. Envisioning biorefineries based on utilization of lignocellulosic straws and bagassess. 227th National Meeting of the American Chemical Society, Anaheim, CA.
HETEROGENEOUS BIOCATALYIC LIQUEFACTION AND SACCHARIFICAATION OF STARCH FOR ETHANOL -
Robertson, G.H., Wong, D., Lee, C.C., Wagschal, K.C. 2004. Heterogeneous biocatalyic liquefaction and saccharificaation of starch for ethanol. Corn Utilization Conference Proceedings, June 6-9, 2004, Indianapolis, Indiana, Abstract.
IN VITRO EVOLUTION OF HIGH-ACTIVITY BARLEY ALPHA-AMYLASE FOR RAW STARCH DIGESTION -
Wong, D., Robertson, G.H., Batt, S.B., Lee, C.C. 2004. In vitro evolution of high-activity barley alpha-amylase for raw starch digestion. American Association of Cereal Chemists Meetings, September 19-22, 2004, San Diego, California. Abstract.
HIGH-ACTIVITY BARLEY ALPHA-AMYLASE BY DIRECTED EVOLUTION -
Wong, D., Batt, S.B., Lee, C.C., Robertson, G.H. 2004. High-activity barley alpha-amylase by directed evolution. Journal of Protein Chemistry, 23:53-460.
APPLYING COMBINATORIAL CHEMISTRY AND BIOLOGY TO FOOD RESEARCH -
Wong, D., Robertson, G.H. 2004. Applying combinatorial chemistry and biology to food research. Journal of Agricultural and Food Chemistry, 52:7187-7198.
RAPID SELECTION OF ALPHA-AMYLASE MUTANTS IN YEAST LIBRARIES FOR RAW STARCH ACTIVITY -
Wong, D., Batt, S.B., Lee, C.C., Robertson, G.H. Rapid selection of alpha-amylase mutants in yeast libraries for raw starch activity. 2003. Society of Biomolecular Screening Annual Conference, Portland, Oregon.
ENGINEERING APHA-AMYLASE FOR ENHANCED ACTIVITY BY MOLECULAR EVOLUTION -
Wong, D., Batt, S.B., Lee, C.C., Robertson, G.H. Engineering apha-amylase for enhanced activity by molecular evolution. American Society of Biochemistry and Molecular Biology Annual Meeting, June 12-16, 2004, Boston, Massachusetts. Abstract No. B38.
AN EPISOMAL EXPRESSION VECTOR FOR SCREENING MUTANT GENE LIBRARIES IN PICHIA PASTORIS -
Lee, C.C., Williams, T.G., Wong, D., Robertson, G.H. 2004. An episomal expression vector for screening mutant gene libraries in pichia pastoris. Plasmid Journal. 54(1):80-85.
DIRECT SCREENING OF LIBRARIES OF YEAST CLONES FOR ALPHA-AMYLASE ACTIVITY ON RAW STARCH -
Wong, D., Batt, S.B., Lee, C.C., Robertson, G.H. 2004. Direct screening of libraries of yeast clones for alpha-amylase activity on raw starch. Protein and Peptide Letters. 10:459-468.
CLONING AND CHARACTERIZATION OF THE XYN11A GENE FROM LENTINULA EDODES -
Lee, C.C., Wong, D., Robertson, G.H. 2005. Cloning and characterization of the xyn11a gene from lentinula edodes. Protein Journal. 24(1):21-26.
PHAGE DISPLAYED PEPTIDE LIGANDS FOR PANCREATIC A-AMYLASE CROSS-REACT WITH BARLEY A-AMYLASE -
PEROXIDASE-CATALYZED CROSSLINKED CASEINATE FILMS -
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Last Modified: 5/2/2016
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