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United States Department of Agriculture

Agricultural Research Service

Related Topics

Kevin B Temeyer
Tick and Biting Fly Research
General Biological Science

Phone: (830) 792-0332
Fax: (830) 792-0302

2700 FREDERCKSBURG ROAD
KERRVILLE , TX 78028

Projects
Genomics of Livestock Pests
Appropriated (D)
  Accession Number: 427176
Flies Associated with Livestock Production Systems
Appropriated (D)
  Accession Number: 427991
Deployed Warfighter Protection Research Program (FY2016)
Interagency Reimbursable Agreement (I)
  Accession Number: 430339
Deployed Warfighter Protection Research Program (FY2015)
Interagency Reimbursable Agreement (I)
  Accession Number: 427381
Deployed Warfighter Protection Research Program (FY2014)
Interagency Reimbursable Agreement (I)
  Accession Number: 424295
Towards Novel Acaricide Development Against Cattle Fever Tick: GPCR Target Validation and Identification of Chemical Leads
Reimbursable (R)
  Accession Number: 430846

Publications (Clicking on the reprint icon Reprint Icon will take you to the publication reprint.)
Persistence of two Salmonella enterica ser. Montevideo strains throughout horn fly (Diptera: Muscidae) larval and pupal development -
Tick salivary acetylcholinesterase: A probable immunomodulator of host-parasite interactions -
Temeyer, K.B., Tuckow, A.P. 2016. Tick salivary acetylcholinesterase: A probable immunomodulator of host-parasite interactions. Journal of Medical Entomology. 53:500-504.
Acetylcholinesterase mutations and organophosphate resistance in sand flies and mosquitoes -
Pharmacological characterization of a tyramine receptor from the southern cattle tick, Rhipicephalus (Boophilus) microplus Reprint Icon -
Gross, A.D., Temeyer, K.B., Day, T.A., Perez De Leon, A.A., Kimber, M.J., Coats, J.R. 2015. Pharmacological characterization of a tyramine receptor from the southern cattle tick, Rhipicephalus (Boophilus) microplus. Insect Biochemistry and Molecular Biology. 63:47-53.
Acetylcholinesterase mutations and organophosphate resistance in sand flies and mosquitoes -
Discovery, adaptation and transcriptional activity of two tick promoters: Construction of a dual luciferase reporter system for optimization of RNA interference in Rhipicephalus (Boophilus) microplus cell lines Reprint Icon -
Tuckow, A.P., Temeyer, K.B. 2015. Discovery, adaptation and transcriptional activity of two tick promoters: Construction of a dual luciferase reporter system for optimization of RNA interference in Rhipicephalus (Boophilus) microplus cell lines. Insect Molecular Biology. 24(4):454-456.
Identification, characterization and use of two tick promoters for construction of a dual luciferase reporter vector -
Acetylcholinesterase of the sand fly, Phlebotomus papatasi (Scopoli): construction, expression and biochemical properties of the G119S orthologous mutant Reprint Icon -
Temeyer, K.B., Tong, F., Totrov, M.M., Tuckow, A.P., Chen, Q., Carlier, P.R., Perez De Leon, A.A., Bloomquist, J.R. 2014. Acetylcholinesterase of the sand fly, Phlebotomus papatasi (Scopoli): construction, expression and biochemical properties of the G119S orthologous mutant. Parasites & Vectors. 7:577.
Genome-based approach to discover new livestock pest control technologies: The horn fly (Haematobia irritans) genome sequencing project -
Recombinant acetylcholinesterase 1 of the sand fly Phlebotomus papatasi (Scopoli): expression, biochemical properties, and insensitivity to organophosphate inhibition -
Toxicity and repellency of plant essential oils against the arthropod disease vectors Phlebotomus papatasi and Ixodes scapularis -
Li, A.Y., Costa-Junior, L.M., Temeyer, K.B., Chauhan, K.R., Rosario-Cruz, R., Perez De Leon, A.A. 2015. Toxicity and repellency of plant essential oils against the arthropod disease vectors Phlebotomus papatasi and Ixodes scapularis. Meeting Abstract Book of the 7th International Conference of Biopesticide. P.44..
Development and validation of a dual luciferase reporter system for in vitro evaluation of gene silencing efficacy in the Southern cattle tick: comparison to in vivo gene silencing by microinjection -
Acaricide resistance and strategies to mitigate economic impact of the southern cattle fever tick (Rhipicephalus microplus) on livestock production systems in the Americas -
Acaricide Resistance in Rhipicephalus (Boophilus) microplus: Impact on Agro-Biosecurity and Cattle Trade between Mexico and the United States of America -
Expression and Biochemical Properties of a Recombinant Acetylcholinesterase 1 of the Sand Fly, Phlebotomus papatasi (Scopoli) Insensitive to Organophosphate Inhibition -
Functional and Pharmacological Characterization of a Tyramine Receptor from the Southern Cattle Tick, Rhipicephalus microplus -
Evaluation of insecticides and repellents for the control of the sand fly Phlebotomus papatasi to protect deployed U.S. Military personnel -
Discovery of microRNAs of the stable fly (Diptera: Muscidae) by high-throughput sequencing -
Tuckow, A.P., Temeyer, K.B., Olafson, P.U., Perez De Leon, A.A. 2013. Discovery of microRNAs of the stable fly (Diptera: Muscidae) by high-throughput sequencing. Journal of Medical Entomology. 50(4):925-930.
Inhibitor Profile of bis(n)-tacrines and N-methylcarbamates on Acetylcholinesterase from Rhipicephalus (Boophilus) microplus and Phlebotomus papatasi -
Swale, D.R., Tong, F., Temeyer, K.B., Li, A.Y., Lam, P., Totrov, M.M., Carlier, P.R., Perez De Leon, A.A., Bloomquist, J.R. 2013. Inhibitor profile of bis(n)-tacrines and N-methylcarbamates on acetylcholinesterase from Rhipicephalus (Boophilus) microplus and Phlebotomus papatasi. Journal of Pesticide Biochemistry and Physiology. 106:85-92.
Acetylcholinesterase of Rhipicephalus (Boophilus) microplus and Phlebotomus papatasi: Gene identification, expression, and biochemical properties of recombinant proteins -
Temeyer, K.B., Olafson, P.U., Brake, D.K., Tuckow, A.P., Li, A.Y., Perez De Leon, A.A. 2013. Acetylcholinesterase of Rhipicephalus (Boophilus) microplus and Phlebotomus papatasi: Gene identification, expression, and biochemical properties of recombinant proteins. Pesticide Biochemistry and Physiology. 106:118-123.
Acetylcholinesterase of the Sand Fly Phlebotomus papatasi (Scopoli): cDNA Sequence, Baculovirus Expression and Biochemical Properties -
Temeyer, K.B., Brake, D.K., Tuckow, A.P., Li, A.Y., Perez De Leon, A.A. 2013. Acetylcholinesterase of the sand fly Phlebotomus papatasi (Scopoli): cDNA sequence, baculovirus expression and biochemical properties. Parasites & Vectors. 6(1):article 31.
Acetylcholinesterases of Blood-feeding Flies and Ticks -
Temeyer, K.B., Tuckow, A.P., Brake, D.K., Li, A.Y., Perez De Leon, A.A. 2013. Acetylcholinesterases of blood-feeding flies and ticks. Chemico Biological Interactions. 203:319-322.
USDA-ARS Knipling-Bushland U.S. Livestock Insects Research Laboratory Tick & Biting Fly Research Unit scientific update -
Discovery and functional analysis of small RNAs (miRNA/siRNA) of livestock ectoparasites. -
The US experience of eradicating and preventing re-infestation with cattle fever ticks -
Li, A.Y., Miller, R., Pound, J.M., Guerrero, F., Temeyer, K.B., Perez De Leon, A.A. 2012. The US experience of eradicating and preventing re-infestation with cattle fever ticks. Workshop Proceedings. 4th International Bayer Cattle Symposium, Lisbon, Portugal, June 3, 2012 (4-19).
Acetylcholinesterases of Rhipicephalus (Boophilus) microplus and Phlebotomus papatasi: Gene identification, expression and biochemical properties of recombinant proteins -
A Novel Carbamate Insecticide with Superior Selectivity for Cattle Tick (Boophilus microplus) and Sand Fly (Phlebotomus papatasi) Control -
Acetylcholinesterase of Haematobia irritans (Diptera: Muscidae): Baculovirus expression, biochemical properties and organophosphate insensitivity -
Temeyer, K.B., Brake, D.K., Schlechte, K.G. 2012. Acetylcholinesterase of Haematobia irritans (Diptera: Muscidae): Baculovirus expression, biochemical properties and organophosphate insensitivity. Journal of Medical Entomology. 49(3):589-594.
Sequence polymorphism in acetylcholinesterase transcripts and genotyping survey of BmAChE1 in laboratory and Mexican strains of Rhipicephalus (Boophilus) microplus -
Temeyer, K.B., Olafson, P.U., Pruett, J.H. 2012. Sequence polymorphism in acetylcholinesterase transcripts and genotyping survey of BmAChE1 in laboratory and Mexican strains of Rhipicephalus (Boophilus) microplus. Journal of Medical Entomology. 49(3):555-562.
Complexity of acetylcholinesterases in biting flies and ticks -
Optimization of RNA interference (RNAi) targeting acetylcholinesterase in the Southern cattle tick (Rhipicephalus microplus) -
Multiple acetylcholinesterase genes expressed in the southern cattle tick, Rhipicephalus (Boophilus) microplus, a vector of bovine babesiosis and anaplasmosis -
Innovative technologies targeting vector populations to mitigate the risk of exposure to leishmaniasis and protect deployed U.S. Military personnel in the Middle East -
Multiple acetylcholinesterase genes expressed in the southern cattle tick, Rhipicephalus (Boophilus) microplus, a vector of bovine anaplasmosis and babesiosis -
Innovative technologies targeting vector populations to mitigate the risk of exposure to leishmaniasis and protect deployed U.S. Military personnel in the Middle East -
Genetic factors potentially reducing fitness cost of organophosphate-insensitive acetylcholinesterase(s) in Rhipicephalus (Boophilus) microplus (Acari: Ixodidae) -
Temeyer, K.B., Tijerina, M.A., Davey, R.B., Olafson, P.U. 2011. Genetic factors potentially reducing fitness cost of organophosphate-insensitive acetylcholinesterase(s) in Rhipicephalus (Boophilus) microplus (Acari: Ixodidae). Acarology International Congress Proceedings. Zoosymposia 6:260–266.
Acetylcholinesterase of Stomoxys calcitrans (L.) (Diptera: Muscidae): cDNA sequence, baculovirus expression, and biochemical properties -
Temeyer, K.B., Chen, A.C. 2012. Acetylcholinesterase of Stomoxys calcitrans (L.) (Diptera: Muscidae): cDNA sequence, baculovirus expression, and biochemical properties. Veterinary Parasitology. 184(1):92-95.
Novel approaches for control of Rhipicephalus (Boophilus) microplus -
Temeyer, K.B., Chen, A.C., Davey, R.B., Guerrero, F., Freeman, J.M., Kammlah, D.M., Li, A.Y., Lohmeyer, K.H., Olafson, P.U., Perez De Leon, A.A., Phillips, P.L., Pound, J.M., Welch, J.B. 2012. Novel approaches for control of Rhipicephalus (Boophilus) microplus. J Tecnica Pecuaria En Mexico. 3(Suppl. 1):25-40.
Four transcripts encode glucose 6-phosphate dehydrogenase (G6PDH) in the Southern cattle tick, Rhipicephalus (Boophilus) microplus -
Multiple transcripts encode glucose 6-phosphate dehydrogenase in the southern cattle tick, Rhipicephalus (Boophilus) microplus Reprint Icon -
Olafson, P.U., Pruett Jr, J.H., Temeyer, K.B. 2011. Multiple transcripts encode glucose 6-phosphate dehydrogenase in the southern cattle tick, Rhipicephalus (Boophilus) microplus. Experimental and Applied Acarology. 53(2):147-165.
Acetylcholinesterases of Rhipicephalus (Boophilus) microplus – Multiple gene expression presents an opportune model system for elucidation of multiple functions of AChEs. -
Shift in rules of engagement for eradication of cattle fever ticks in the United States -
Baculovirus expression, biochemical characterization and organophosphate sensitivity of rBmAChE1, rBmAChE2, and rBmAChE3 of Rhipicephalus (Boophilus) microplus -
Temeyer, K.B., Pruett Jr, J.H., Olafson, P.U. 2010. Baculovirus expression, biochemical characterization and organophosphate sensitivity of rBmAChE1, rBmAChE2, and rBmAChE3 of Rhipicephalus (Boophilus) microplus. Veterinary Parasitology. 172:114-121.
Genotyping mutation in BmAChE3: A survey of laboratory and Mexican strains of Rhipicephalus (Boophilus) microplus that are resistant or susceptible to coumaphos -
Temeyer, K.B., Olafson, P.U., Miller, R. 2009. Genotyping mutation in BmAChE3: A survey of laboratory and Mexican strains of Rhipicephalus (Boophilus) microplus that are resistant or susceptible to coumaphos. Journal of Medical Entomology. 46(6):1355-1360.
Nutritional Limitation on Growth and Development of Horn Fly (Diptera:Muscidae) Larvae -
Temeyer, K.B. 2009. Nutritional limitation on growth and development of horn fly (Diptera:Muscidae) larvae. Southwestern Entomologist. 34(3):263-272.
A survey of Rhipicephalus microplus populations for mutations associated by pyrethroid resistance -
Chen, A.C., He, H., Temeyer, K.B., Jones, S., Green, P., Barker, S.C. 2009. A survey of Rhipicephalus microplus populations for mutations associated by pyrethroid resistance. Journal of Economic Entomology. 120(1):373-380.
ACETYLCHOLINESTERASE MUTATION IN DIAZINON-RESISTANT HAEMATOBIA IRRITANS (L.) (DIPTERA: MUSCIDAE) -
Temeyer, K.B., Li, A.Y., Lohmeyer, K.H., Chen, A.C., Olafson, P.U., Sanson, D.W., Foil, L.D. 2008. Acetylcholinesterase mutation in diazinon-resistant Haematobia irritans (L.) (Diptera: Muscidae). Veterinary Parasitology. 154(3-4):300-310.
R86Q, a mutation in BmAChE3 yielding a Rhipicephalus microplus organophosphate-insensitive acetylcholinesterase -
Temeyer, K.B., Pruett Jr, J.H., Olafson, P.U., Chen, A.C. 2007. R86Q, a mutation in BmAChE3 yielding a Rhipicephalus microplus organophosphate-insensitive acetylcholinesterase. Journal of Medical Entomology. 44(6):1013-1018.
Identification and characterization of a cDNA encoding the acetylcholinesterase of Haematobia irritans (L.) (Diptera: Muscidae) -
Temeyer, K.B., Chen, A.C. 2007. Identification and characterization of a cDNA encoding the acetylcholinesterase of Haematobia irritans (L.) (Diptera: Muscidae). DNA Sequence. 18(2):85-91.
BACULOVIRUS EXPRESSION OF BMACHE3, A CDNA ENCODING AN ACETYLCHOLINESTERASE OF BOOPHILUS MICROPLUS (ACARI:IXODIDAE) -
Temeyer, K.B., Pruett Jr., J.H., Untalan, P.M., Chen, A.C. 2006. Baculovirus expression of BmAChE3, a cDNA encoding an acetylcholinesterase of Boophilus microplus (Acari: Ixodidae). Journal of Medical Entomology. 43(4):707-712.
ORGANOPHOSPHATE RESISTANCE IN MEXICAN STRAINS OF BOOPHILUS MICROPLUS: A MAJOR THREAT TO THE U.S. CATTLE INDUSTRY -
Temeyer, K.B., Pound, J.M., Miller, J.A., Chen, A.C., Pruett Jr, J.H., Guerrero, F., Davey, R.B., Untalan, P.M., Lohmeyer, K.H., Li, A.Y., Miller, R., George, J.E. 2004. Organophosphate resistance in Mexican strains of Boophilus microplus: A major threat to the U.S. cattle industry. Southern Association of Agricultural Scientists Bulletin of Biochemistry and Biotechnology. 17:43-51.
IDENTIFICATION OF A THIRD BOOPHILUS MICORPLUS (ACARI: IXODIDAE) CDNA PRESUMPTIVELY ENCODING AN ACETYLCHOLINESTERASE -
Temeyer, K.B., Davey, R.B., Chen, A.C. 2004. Identification of a third Boophilus microplus (Acari: Ixodidae) cDNA presumptively encoding an acetylcholinesterase. Journal of Medical Entomology. 41(3):259-268.
CLONING AND SEQUENCE ANALYSIS OF A CDNA ENCODING PSO O II, A MITE GROUP 2 ALLERGEN OF THE SHEEP SCAB MITE, PSOROPTES OVIS (ACARI:PSORPTIDAE) -
TEMEYER, K.B., SOILEAU, L.C., PRUETT JR, J.H. CLONING AND SEQUENCE ANALYSIS OF A CDNA ENCODING PSO O II, A MITE GROUP 2 ALLERGEN OF THE SHEEP SCAB MITE, PSOROPTES OVIS (ACARI:PSORPTIDAE). JOURNAL OF MEDICAL ENTOMOLOGY. v. 39. p. 384-391.
NUTRIENT DEPLETION OF BOVINE DUNG AND DENSITY-DEPENDENT STUNTING PRODUCE EQUIVALENT NUTRITIONAL LIMITATION OF HORNFLY LARVAL GROWTH -
PRODUCTION OF MONOCLONAL ANTIBODIES SPECIFIC FOR HYPODERMIN C, A COLLAGENASE OF THE COMMON CATTLE GRUB, HYPODERMA LINEATUM -
CONSTRUCTION OF HYBRIDOMAS WHICH SECRETE MONOCLONAL ANTIBODIES SPECIFIC FORANTIGENS OF THE SHEEP SCAB MITE, PSOROPTES OVIS -
EVALUATION OF NATURAL PSOROPTES OVIS (HERING) (ACARINA: PSOROPTIDAE) SOLUBLE PROTEINS AS CANDIDATE VACCINE IMMUNOGENS -
ANTIFUNGAL AGENTS AND SOLVENTS FOR USE IN HORN FLY LARVAL REARING OR BIOASSAYS -
CONSTRUCTION OF MURINE HYBRIDOMAS WHICH SECRETE MONOCLONAL ANTIBODIES SPECIFIC FOR HYPODERMIN A, A SERINE PROTEASE OF THE COMMON CATTLE GRUB, HYPODERMA LINEATUM -
CONSTRUCTION OF MURINE HYBRIDOMAS WHICH SECRETE MONOCLONAL ANTIBODIES SPECIFIC FOR HYPODERMIN C, A COLLAGENASE OF THE COMMON CATTLE GRUB, HYPODERMA LINEATUM -
Sequence 2 from patent EP 0326419 -
Pruett Jr, J.H., Files, J.G., Kuhn, I., Temeyer, K.B. 1994. Sequence 2 from patent EP 0326419. National Center for Biotechnology Information (NCBI). GenBank Sequence Submission Accession #AAA55755. Available: www.ncbi.nlm.nih.gov/gquery/?term=AAA55755
Sequence 8 from patent EP 0326419 -
Pruett Jr, J.H., Files, J.G., Kuhn, I., Temeyer, K.B. 1994. Sequence 8 from patent EP 0326419. National Center for Biotechnology Information (NCBI). GenBank sequence submission Accession # AAA55758. Available: http://www.ncbi.nlm.nih.gov/gquery/?term=AAA55758.
hypodermin A, partial [Hypoderma lineatum] -
Kuhn, I., Files, J.G., Pruett Jr, J.H., Temeyer, K.B. 1993. hypodermin A, partial [Hypoderma lineatum]. National Center for Biotechnology Information (NCBI). GenBank Accession Sequence Submission #AAA29224. Available: www.ncbi.nlm.nih.gov/gquery/?term=AAA29224
Last Modified: 5/2/2016
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