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United States Department of Agriculture

Agricultural Research Service

Related Topics

Claudio L Afonso
Exotic & Emerging Avian Viral Diseases Research
Microbiology

Phone: (706) 546-3642
Fax: (706) 546-3161

USDA, ARS, SEPRL
934 COLLEGE STATION ROAD
SPRL
ATHENS , GA 30605

Projects
Intervention Strategies to Predict, Prevent and Control Disease Outbreaks Caused by Emerging Strains of Virulent Newcastle Disease Viruses
Appropriated (D)
  Accession Number: 431889
Surveillance and Molecular Epidemiology of NDV in Kenya
Interagency Reimbursable Agreement (I)
  Accession Number: 429925
Genomic, Epidemiological and Biological Characterization of Newcastle Disease Virus Isolates from Ukraine
Interagency Reimbursable Agreement (I)
  Accession Number: 425414
Development of Vaccines Against Newcastle Disease Using Recombinant DNA Technology and Study of Their Proective Effects in Chickens
Reimbursable (R)
  Accession Number: 426325
Surveillance and Molecular Epidemiology of Newcastle Disease in Kenya
Specific Cooperative Agreement (S)
  Accession Number: 430638
Surveillance and Molecular Epidemiology of NDV in Kenya
Specific Cooperative Agreement (S)
  Accession Number: 431417
Evaluation of Recombinant Newcastle Disease Vaccines (NDV)
Trust (T)
  Accession Number: 426432
Generation of the Bivalent Vaccine Against Newcastle Disease (ND) and Infectious Laryngotracheitis (ILT)
Trust (T)
  Accession Number: 428130

Publications (Clicking on the reprint icon Reprint Icon will take you to the publication reprint.)
Repeated isolation of virulent Newcastle disease viruses of sub-genotype VIId from backyard chickens in Bulgaria and Ukraine between 2002 and 2013 -
Dimitrov, K.M., Bolotin, V., Muzyka, D., Goraichuk, I., Solodiankin, O., Gerilovych, A., Stegniy, B., Goujgoulova, G., Silko, N., Pantin Jackwood, M.J., Miller, P.J., Afonso, C.L. 2016. Repeated isolation of virulent Newcastle disease virus of genotype VII d from backyard chickens in Bulgaria and Ukraine between 2002 and 2013. Archives of Virology. doi: 10.1007/s00705-016-3033-2.
Presence of vaccine-derived newcastle disease viruses in wild birds Reprint Icon -
Ayala, A.J., Dimitrov, K.M., Becker, C.R., Goraichuk, I.V., Arns, C.W., Bolotin, V.I., Ferreira, H.L., Gerilovych, A.P., Goujgoulova, G.V., Miller, P.J., Afonso, C.L. 2016. Presence of vaccine-derived newcastle disease viruses in wild birds. PLoS One. 11(9):1-19 doi:10.1371/journal.pone.0162484.
Effects of chicken interferon Gamma on Newcastle disease virus vaccine immunogenicity Reprint Icon -
Cardenas-Garcia, S., Dunwoody, R.P., Marcano, V., Diel, D.G., Williams, R.J., Gogal Jr., R.M., Brown, C.C., Miller, P.J., Afonso, C.L. 2016. Effects of chicken interferon Gamma on Newcastle disease virus vaccine immunogenicity. PLoS One. 11(7):e0159153. doi: 10.1371/journal.pone.0159153.
Complete Genome Sequence of an Avian Paramyxovirus Representative of Putative New Serotype 13 -
Goraichuk, I.V., Sharma, P., Stegniy, B., Muzyka, D., Pantin Jackwood, M.J., Gerilovych, A., Solodiankin, O., Vitaliy, B., Miller, P.J., Dimitrov, K.M., Afonso, C.L. 2016. Complete Genome Sequence of an Avian Paramyxovirus Representative of Putative New Serotype 13. Genome Announcements. 4(4):e00729-16. Available: http://genomea.asm.org/content/4/4/e00729-16.full.
H9N2 low pathogenic avian influenza in Pakistan (2012-2015) Reprint Icon -
Lee, D., Swayne, D.E., Sharma, P., Rehmani, S.F., Wajid, A., Suarez, D.L., Afonso, C.L. 2016. H9N2 low pathogenic avian influenza in Pakistan (2012-2015). Veterinary Record. 3:e000171. doi: 10.1136/vetreco-2016-000171.
Taxonomy of the order Mononegavirales: update 2016 Reprint Icon -
Afonso, C.L., Amarasinghe, G.K., Banyai, K., Bao, Y., Basler, C.F., Bavari, S., Bejerman, N., Blasdell, K.R., Briand, F., Kuhn, J.H., Domier, L.L. 2016. Taxonomy of the order Mononegavirales: update 2016. Archives of Virology. 161:2351-2360. doi: 10.1007/s00705-016-2880-1.
Pathogenesis of new sub-genotypes of Newcastle disease virus strains from Israel and Pakistan Reprint Icon -
Pandarrangga, P., Brown, C.C., Miller, P.J., Haddas, R., Rehmani, S.F., Afonso, C.L., Susta, L. 2016. Pathogenesis of new sub-genotypes of Newcastle disease virus strains from Israel and Pakistan. Veterinary Pathology. 53(4)792-796 doi: 10.1177/0300985815622972.
Identification of Avian Paramyxovirus Serotype-1 in Wild Birds in the USA Reprint Icon -
Pedersen, K., Marks, D.R., Afonso, C.L., Stopak, S.R., Williams Coplin, T.D., Dimitrov, K.M., Miller, P.J., Deliberto, T.J. 2016. Identification of Avian Paramyxovirus Serotype-1 in Wild Birds in the USA. Journal of Wildlife Diseases. 52(3). doi: http://dx.doi.org/10.7589/2015-10-278.
Temporal, geographic, and host distribution of avian paramyxovirus 1 (Newcastle disease virus) Reprint Icon -
Dimitrov, K.M., Ramey, A.M., Qiu, X., Bahl, J., Afonso, C.L. 2016. Temporal, geographic, and host distribution of avian paramyxovirus 1 (Newcastle disease virus). Infection, Genetics and Evolution. 39(1)22-34 doi: 10.1016/j.meegid.2016.01.008.
Complete genome sequence of a genotype XVII Newcastle disease virus, isolated from an apparently healthy domestic duck in Nigeria Reprint Icon -
Shittu, I., Sharma, P., Joannis, T.M., Volkening, J.D., Odaibo, G.N., Olaleye, D.O., Williams Coplin, T.D., Solomon, P., Abolnik, C., Miller, P.J., Dimitrov, K.M., Afonso, C.L. 2016. Complete genome sequence of a genotype XVII Newcastle disease virus, isolated from an apparently healthy domestic duck in Nigeria. Genome Announcements. 4(1). Available: http://genomea.asm.rg/content/4/1/e01716-15.full.
Identification and complete genome sequence analysis of a genotype XIV Newcastle disease virus from Nigeria Reprint Icon -
Shittu, I., Sharma, P., Volkening, J.D., Solomon, P., Sulaiman, L.K., Joannis, T.M., Williams Coplin, T.D., Dimitrov, K.M., Miller, P.J., Afonso, C.L. 2016. Identification and complete genome sequence analysis of a genotype XIV Newcastle disease virus from Nigeria. Genome Announcements. 4(1). Available: http://genomea.asm.org/content/4/1/e01581-15.
Evaluation of the transcriptional status of host cytokines and viral genes in the trachea of vaccinated and nonvaccinated chickens after challenge with infectious laryngotracheitis virus (ILTV) Reprint Icon -
Vagnozzi, A., Riblet, S., Zavala, G., Ecco, R., Afonso, C.L., Garcia, M. 2016. Evaluation of the transcriptional status of host cytokines and viral genes in the trachea of vaccinated and nonvaccinated chickens after challenge with infectious laryngotracheitis virus (ILTV). Avian Pathology. 45(1)106:113 doi:10.1080/03079457.2015.1126804.
Effect of Infection with a Mesogenic Strain of Newcastle Disease Virus on Infection with Highly Pathogenic Avian Influenza Virus in Chickens -
Costa Hurtado, M., Afonso, C.L., Miller, P.J., Shepherd, E.M., Dejesus, E., Smith, D.M., Pantin Jackwood, M.J. 2016. Effect of Infection with a Mesogenic Strain of Newcastle Disease Virus on Infection with Highly Pathogenic Avian Influenza Virus in Chickens. Avian Diseases. 60(1):269-78. doi: 10.1637/11171-051915-Reg.
International collaborations to facilitate a better understanding of Newcastle disease epidemiology Reprint Icon -
Miller, P.J., Dimitrov, K.M., Williams Coplin, T.D., Peterson, M.P., Pantin Jackwood, M.J., Swayne, D.E., Suarez, D.L., Afonso, C.L. 2015. International collaborations to facilitate a better understanding of Newcastle disease epidemiology. FRONTIERS IN PUBLIC HEALTH. 3(235)1:10.
Development, characterization, and optimization of a new suspension chicken-induced pluripotent stem cell line for the production of Newcastle disease vaccine -
Shittu, I., Zhu, Z., Stice, S.L., West, F.D., Donadeu, M., Dungu, B., Fadly, A.M., Afonso, C.L. 2015. Development, characterization, and optimization of a new suspension chicken-induced pluripotent stem cell line for the production of Newcastle disease vaccine. Biologicals. 44(1)24-32 doi: 10.1016/j.biologicals.2015.09.002.
Expression of chicken interleukin-2 by a highly virulent strain of Newcastle disease virus leads to decreased systemic viral load but does not significantly affect mortality in chickens -
Susta, L., Diel, D., Courtney, S., Cardenas-Garcia, S., Sundick, R., Miller, P.J., Brown, C., Afonso, C.L. 2015. Expression of chicken interleukin-2 by a highly virulent strain of Newcastle disease virus leads to decreased systemic viral load but does not significantly affect mortality in chickens. Virology Journal. 12:122. DOI: 10.1186/s12985-015-0353-x.
Previous infection with virulent strains of Newcastle disease virus reduces highly pathogenic avian influenza virus replication, disease, and mortality in chickens Reprint Icon -
Costa-Hurtado, M., Afonso, C.L., Miller, P.J., Shepherd, E.M., Cha, R., Smith, D.M., Spackman, E., Kapczynski, D.R., Suarez, D.L., Swayne, D.E., Pantin Jackwood, M.J. 2015. Previous infection with virulent strains of Newcastle disease virus reduces highly pathogenic avian influenza virus replication, disease, and mortality in chickens. Veterinary Research. 46:97. doi: 10.1186/s13567-015-0237-5.
Neuropathogenic capacity of lentogenic, mesogenic, and velogenic Newcastle disease virus strains in day-old chickens -
Moura, V., Susta, L., Cardenas-Garcia, S., Stanton, J., Miller, P.J., Afonso, C.L., Brown, C. 2015. Neuropathogenic capacity of lentogenic, mesogenic, and velogenic Newcastle disease virus strains in day-old chickens. Veterinary Pathology. doi: 10.1177/0300985815600504.
Recombinant newcastle disease vaccines: risk for recombination, reversion to virulence, and spread in non-target species -
Complete genome sequence of a recent panzootic virulent Newcastle disease virus from Pakistan Reprint Icon -
Wajid, A., Wasim, M., Rehmani, S., Bibi, T., Ahmed, N., Afonso, C.L. 2015. Complete genome sequence of a recent panzootic virulent Newcastle disease virus from Pakistan. Genome Announcements. 3(3):e00658-15. doi: 10.1128/genomeA.00658-15.
Delayed Newcastle disease virus replication using RNA interference to target the nucleoprotein Reprint Icon -
Hutcheson, J.M., Susta, L., Stice, S.L., Afonso, C.L., West, F.D. 2015. Delayed Newcastle disease virus replication using RNA interference to target the nucleoprotein. Biologicals. 43(4):274-280. doi: 10.1016/j.biologicals.2015.03.004.
Experimental co-infections of domestic ducks with a virulent Newcastle disease virus and low or highly pathogenic avian influenza viruses Reprint Icon -
Pantin Jackwood, M.J., Costa-Hurtado, M., Miller, P.J., Afonso, C.L., Spackman, E., Kapczynski, D.R., Shepherd, E.M., Smith, D.M., Swayne, D.E. 2015. Experimental co-infections of domestic ducks with a virulent Newcastle disease virus and low or highly pathogenic avian influenza viruses. Veterinary Microbiology. 177:7-17.
Infection and transmission of live recombinant Newcastle disease virus vaccines in Rock Pigeons, European House Sparrows, and Japanese Quail -
Miller, P.J., Diel, D.G., Hamal, K.R., Olivier, T.L., Cardenas-Garcia, S., Marcano, V.C., Afonso, C.L., Suarez, D.L. 2015. Infection and transmission of live recombinant Newcastle disease virus vaccines in Rock Pigeons, European House Sparrows, and Japanese Quail [abstract]. American Veterinary Medical Association. p. 122.
Presence of virulent Newcastle disease virus in vaccinated chickens in farms in Pakistan Reprint Icon -
Rehmani, S.F., Wajid, A., Bibi, T., Nazir, B., Mukhtar, N., Hussain, A., Lone, N., Yaqub, T., Afonso, C.L. 2015. Presence of virulent Newcastle disease virus in vaccinated chickens in farms in Pakistan. Journal of Clinical Microbiology. 53(5):1715-1718. doi: 10.1128/JCM.02818-14.
Newcastle disease viruses causing recent outbreaks worldwide show unexpectedly high genetic similarity with historical virulent isolates from the 1940s Reprint Icon -
Dimitrov, K.M., Lee, D., Williams Coplin, T.D., Olivier, T.L., Miller, P.J., Afonso, C.L. 2016. Newcastle disease viruses causing recent outbreaks worldwide show unexpectedly high genetic similarity with historical virulent isolates from the 1940s. Journal of Clinical Microbiology. 54(5)1228-1235 doi: 10.1128/JCM.03044-15.
Dynamics of House Sparrows (Passer domesticus) in Newcastle disease virus transmission within the avifaunal-poultry interface: an epidemiological modeling approach -
Evaluation of the infection and transmission of wild type and recombinant strains of Newcastle disease virus in Japanese Quail -
Previous infection with a mesogenic strain of Newcastle disease virus affects infection with highly pathogenic avian influenza viruses in chickens -
Costa Hurtado, M., Afonso, C.L., Miller, P.J., Shepherd, E.M., Smith, D.M., Pantin Jackwood, M.J. 2015. Previous infection with a mesogenic strain of Newcastle disease virus affects infection with highly pathogenic avian influenza viruses in chickens [abstract]. 9th International Symposium on Avian Influenza, Athens, Georgia. p.87.
Development of an improved vaccine evaluation protocol to compare the efficacy of Newcastle disease vaccines Reprint Icon -
Cardenas-Garcia, S., Diel, D., Susta, L., Lucio-Decanini, E., Yu, Q., Brown, C.C., Miller, P.J., Afonso, C.L. 2014. Development of an improved vaccine evaluation protocol to compare the efficacy of Newcastle disease vaccines. Biologicals. 43:136-145. doi: 10.1016/j.biologicals.2014.11.003.
Identification of new sub-genotypes of virulent Newcastle disease virus with potential panzootic features Reprint Icon -
Miller, P.J., Haddas, R., Simanov, L., Lublin, A., Rehmani, S.F., Wajid, A., Bibi, T., Khan, T.A., Yaqub, T., Setiyaningsih, S., Afonso, C.L. 2015. Identification of new sub-genotypes of virulent Newcastle disease virus with potential panzootic features. Infection, Genetics and Evolution. 29:216-229.
Assessment of NDV neuropathogenesis through the ICPI model -
Pathological and phylogenetic characterization of Newcastle disease viruses from Israel and Pakistan -
Wild bird surveillance for avian paramyxoviruses in the Azov-Black Sea Region of Ukraine (2006 to 2011) reveals epidemiological connections with Europe and Africa Reprint Icon -
Muzyka, D., Pantin Jackwood, M.J., Stegniy, B., Rula, O., Bolotin, V., Stegniy, A., Gerilovych, A., Shutchenko, P., Stegniy, M., Koshelev, V., Maiorova, K., Tkachenko, S., Muzyka, N., Usova, L., Afonso, C.L. 2014. Wild bird surveillance for avian paramyxoviruses in the Azov-Black Sea Region of Ukraine (2006 to 2011) reveals epidemiological connections with Europe and Africa. Applied and Environmental Microbiology. 80(17):5427-5438. DOI: 10.1128/AEM.00733-14.
Experimental co-infection of chickens with lentogenic, mesogenic and velogenic strains of Newcastle disease viruses and highly pathogenic avian influenza viruses -
Pantin Jackwood, M.J., Costa-Hurtado, M., Afonso, C.L., Miller, P.J., Shepherd, E.M., Smith, D.M. 2014. Experimental co-infection of chickens with lentogenic, mesogenic and velogenic strains of Newcastle disease viruses and highly pathogenic avian influenza viruses [abstract}. The Fifth ESWI Influenza Conference, September 14-17, 2014, Riga, Latvia. Paper No: SPB2P12. P50.
Improving newcastle disease vaccination with homologous vaccines -
Cardenas Garcia, S., Miller, P.J., Brown, C.C., Afonso, C.L. 2014. Improving newcastle disease vaccination with homologous vaccines [abstract]. American Veterinary Medical Association. Abstract/Paper ID No. 16714. p. 59.
Newcastle disease: current vaccine research -
Cardenas Garcia, S., Miller, P.J., Afonso, C.L. 2014. Newcastle disease: current vaccine research [abstract]. Abstracts for the Pre-Congress, for ANECA p. 28.
Separate evolution of virulent newcastle disease virus from Mexico and Central America Reprint Icon -
Susta, L., Hamal, K.R., Miller, P.J., Garcia, S.C., Brown, C.C., Pedersen, J.C., Gongora, V., Afonso, C.L. 2014. Separate evolution of virulent newcastle disease virus from Mexico and Central America. Journal of Clinical Microbiology. 52(5):1382-1390. DOI: 10.1128/JCM.00066-14.
Avian paramyxovirus serotype 1 (Newcastle disease virus), avian influenza virus and salmonella spp. in mute swans (Cygnus olor) in the great lakes region and atlantic coast of the United States Reprint Icon -
Pedersen, K., Marks, D.R., Arsnoe, D.M., Afonso, C.L., Bevins, S.N., Miller, P.J., Randall, A.R., Deliberto, T.L. 2014. Avian paramyxovirus serotype 1 (Newcastle disease virus), avian influenza virus and salmonella spp. in mute swans (Cygnus olor) in the great lakes region and atlantic coast of the United States. Avian Diseases. 58(1):129-136. DOI: 10.1637/10638-081413-Reg.1
Pathologic characterization of genotypes XIV and XVII Newcastle disease viruses and efficacy of classical vaccination on specific pathogen-free birds Reprint Icon -
Susta, L., Jones, M.E., Cattoli, G., Cardenas-Garcia, S., Miller, P.J., Brown, C.C., Afonso, C.L. 2014. Pathologic characterization of genotypes XIV and XVII Newcastle disease viruses and efficacy of classical vaccination on specific pathogen-free birds. Veterinary Pathology. DOI: 10.1177/0300985814521247.
Emergence of new sub-genotypes of virulent Newcastle disease virus with panzootic features -
Miller, P.J., Bubu, T., Haddas, R., Khan, T.A., Mukhar, N., Rehmani, S.F., Setiyaningsih, S., Wajid, A., Yaqub, T., Zahid, M.N., Afonso, C.L. 2014. Emergence of new sub-genotypes of virulent Newcastle disease virus with panzootic features [abstract]. American Veterinary Medical Association. Abstract/Paper ID No. 16731. p. 59.
Recombinant Newcastle disease vaccines: risk for recombination, reversion to virulence, and spread in non-target species -
Newcastle disease B1 vaccine strain in wild rock pigeons in Atlanta, Georgia -
Host antiviral defenses induced by a mesogenic strain of Newcastle disease virus prevents infection with a highly pathogenic avian influenza virus in chickens -
Costa-Hurtado, M., Afonso, C.L., Miller, P.J., Shepherd, E.M., Smith, D.M., Pantin Jackwood, M.J. 2014. Host antiviral defenses induced by a mesogenic strain of Newcastle disease virus prevents infection with a highly pathogenic avian influenza virus in chickens [abstract]. American Association of Avian Pahologists Annual Meeting, July 26-29, 2014, Denver, Colorado. CD-ROM.
Capturing enveloped viruses on affinity grids for downstream cryo-electron microscopy applications Reprint Icon -
Kiss, G., Chen, X., Brindley, M., Campbell, P., Afonso, C.L., Ke, Z., Holl, J., Guerrero-Ferreira, R., Byrd-Leotis, L., Steel, J., Steinhauer, D., Plemper, R., Kelly, D., Spearman, P., Wright, E. 2014. Capturing enveloped viruses on affinity grids for downstream cryo-electron microscopy applications. Microscopy and Microanalysis. 20:164-174.
Co-infection of mallards with low virulence Newcastle disease virus and low pathogenic avian influenza virus Reprint Icon -
Franca, M., Howerth, E.W., Carter, D., Byas, A., Poulson, R., Afonso, C.L., Stallknecht, E. 2014. Co-infection of mallards with low virulence Newcastle disease virus and low pathogenic avian influenza virus. Avian Pathology. 43(1):96-104.
Virus interference between H7N2 low pathogenic avian influenza virus and lentogenic Newcastle disease virus in experimental co-infections in chickens and turkeys Reprint Icon -
Costa-Hurtado, M., Afonso, C.L., Miller, P.J., Spackman, E., Kapczynski, D.R., Swayne, D.E., Shepherd, E.M., Smith, D.M., Zsak, A., Pantin Jackwood, M.J. 2014. Virus interference between H7N2 low pathogenic avian influenza virus and lentogenic Newcastle disease virus in experimental co-infections in chickens and turkeys. Veterinary Research. 45:1.
Pathological aspects of NDV infection in the ICPI model -
Previous infection with a mesogenic strain of newcastle disease virus prevents infection with a highly pathogenic avian influenza virus in chickens -
Costa-Hurtado, M., Afonso, C.L., Miller, P.J., Shepherd, E.M., Smith, D.M., Pantin Jackwood, M.J. 2014. Previous infection with a mesogenic strain of newcastle disease virus prevents infection with a highly pathogenic avian influenza virus in chickens [abstract]. International Poultry Scientific Forum, January 27028, 2014, Atlanta, GA. CDROM.
Effects of Newcastle disease virus vaccine antibodies on the shedding and transmission of challenge viruses -
Effect of genotype specific live recombinant Newcastle disease vaccines on virus shedding after challenge -
Experimental co-infection of poultry with avian influenza and newcastle disease virus -
Pantin Jackwood, M.J., Afonso, C.L., Miller, P.J., Spackman, E., Kapczynski, D.R., Shepherd, E.M., Smith, D.M., Cha, R., Costa-Hurtado, M., Suarez, D.L., Swayne, D.E. 2013. Experimental co-infection of poultry with avian influenza and Newcastle disease virus [abstract]. Centers for Excellence in Influenza Research and Surveillance Annual Meeting. p. 64.
Effects of Newcastle disease virus vaccine antibodies on the shedding and transmission of challenge viruses Reprint Icon -
Miller, P.J., Afonso, C.L., El Attrache, J., Dorsey, K.M., Courtney, S.C., Guo, Z., Kapczynski, D.R. 2013. Effects of Newcastle disease virus vaccine antibodies on the shedding and transmission of challenge viruses. Developmental and Comparative Immunology. 41(4):505-513. DOI:S0145-305X(13)00170-5. 10.1016/j.dci.2013.06.007.
Molecular epidemiology of Newcastle disease in Mexico and the potential spillover of viruses from poultry into wild bird species Reprint Icon -
Cardenas-Garcia, S., Navarro Lopez, R., Morales, R., Olvera, M., Marquez, M., Merino, R., Miller, P.J., Afonso, C.L. 2013. Molecular epidemiology of Newcastle disease in Mexico and the potential spillover of viruses from poultry into Wild Bird Species. Applied and Environmental Microbiology. 79(16):4985-4992.
Expression of interferon gamma by a highly virulent Newcastle disease virus decreases its pathogenicity in chickens Reprint Icon -
Susta, L., Cornax, I., Diel, D.G., Cardenas, S., Miller, P.J., Brown, C.C., Afonso, C.L. 2013. Expression of interferon gamma by a highly virulent Newcastle disease virus decreases its pathogenicity in chickens. Microbial Pathogenesis. 62:73-83.
Genetic diversity and mutation of avian paramyxovirus serotype 1 (Newcastle disease virus) in wild birds and evidence for intercontinental spread Reprint Icon -
Ramey, A.M., Reeves, A.B., Ogawa, H., Ip, H.S., Imai, K., Bui, V., Yamaguchi, E., Silko, N.Y., Afonso, C.L. 2013. Genetic diversity and mutation of avian paramyxovirus serotype 1 (Newcastle disease virus) in wild birds and evidence for intercontinental spread. Archives of Virology. 158:2495-2503, 2013 CDROM.
Development of a low-dose fast-dissolving tablet formulation of Newcastle disease vaccine for low-cost backyard poultry immunisation Reprint Icon -
Lal, M., Zhu, C., McClurkan, C., Koelle, D., Miller, P., Afonso, C., Donadeu, M., Dungu, B., Chen D. 2014. Development of a low-dose fast-dissolving tablet formulation of Newcastle disease vaccine for low-cost backyard poultry immunisation. Veterinary Record. DOI: 10.1136/vr.101926.
Immune responses of poultry to newcastle disease virus Reprint Icon -
Kapczynski, D.R., Afonso, C.L., Miller, P.J. 2013. Immune responses of poultry to newcastle disease virus. Developmental and Comparative Immunology. 41(3):447-453. Available: http://dx.doi.org/10.1016/j.dci.2013.04.012
Newcastle disease virus (velogens) -
Afonso, C.L., Miller, P.J. 2014. Newcastle disease virus (velogens). In: Liu, D, editor. Manual of Security Sensitive Microbes and Toxins. New York, NY: CRC Press. p. 689-702.
Experimental co-infection of chickens and turkeys with avian influenza and newcastle disease viruses -
Pantin Jackwood, M.J., Miller, P.J., Afonso, C.L., Spackman, E., Kapczynski, D.R., Shepherd, E.M., Smith, D.M., Cha, R., Swayne, D.E. 2013. Experimental co-infection of chickens and turkeys with avian influenza and newcastle disease viruses [abstract]. American Association of Avian Pathologists Meeting, July 20-23,2013, Chicago, IL. CDROM.
Newcastle disease virus fusion and haemagglutinin-neuraminidase proteins contribute to its macrophage host range Reprint Icon -
Cornax, I., Diel, D.G., Rue, C.A., Estevez, C., Yu, Q., Miller, P.J., Afonso, C.L. 2013. Newcastle disease virus fusion and haemagglutinin-neuraminidase proteins contribute to its macrophage host range. Journal of General Virology. 94:1189-1194.
Avian Paramyxovirus serotypes circulating in wild bird populations of the Azov-Black Sea region of Ukraine in 2006-2011 -
Muzyka, D., Pantin Jackwood, M.J., Stegniy, B., Afonso, C.L. 2013. Avian Paramyxovirus serotypes circulating in wild bird populations of the Azov-Black Sea region of Ukraine in 2006-2011 [abstract]. XVIII Congress of the World Veterinary Poultry Association, August 19-23, 2013, Nantes, France. p. 482.
Development of a genotype specific live Newcastle disease vaccine by replacing the fusion (F) and hemagglutinin-neuramindase (HN) genes into a LaSota vaccine backbone -
Cardenas-Garcia, S., Diel, D.G., Susta, L., Lucio-Decanini, E., Brown, C., Yu, Q., Miller, P.J., Afonso, C.L. 2013. Development of a genotype specific live Newcastle disease vaccine by replacing the fusion (F) and hemagglutinin-neuramindase (HN) genes into a LaSota vaccine backbone. International Poultry Forum Proceedings. p. 28.
Complete genome sequences of new emerging Newcastle disease virus strains isolated from China Reprint Icon -
Hualei, L., Lv, Y., Afonso, C.L., Ge, S., Zheng, D., Zhao, Y., Wang, Z. 2013. Complete genome sequences of new emerging Newcastle disease virus strains isolated from China. Genome Announcements. 1(1):1. CDROM.
Experimental risk assessment of recombinant Newcastle disease virus vaccines -
Miller, P.J., Diel, D.G., Hamal, K., Afonso, C.L., Suarez, D.L. 2013. Experimental risk assessment of recombinant Newcastle disease virus vaccines [abstract]. American Association of Avian Pathologists. CDROM.
Newcastle Disease: Progress and gaps in the development of vaccines and diagnostic tools Reprint Icon -
Afonso, C.L., Miller, P.J. 2013. Newcastle Disease: Progress and gaps in the development of vaccines and diagnostic tools. Developments in Biologicals. 135:95-106.
Highly divergent virulent isolates of newcastle disease virus from the Dominican Republic are members of a new genotype that may have evolved unnoticed for over two decades Reprint Icon -
Courtney, S.C., Gomez, D., Hines, N.L., Pedersen, J.C., Miller, P.J., Afonso, C.L., Susta, L., Brown, C. 2013. Highly divergent virulent isolates of newcastle disease virus from the Dominican Republic are members of a new genotype that may have evolved unnoticed for over two decades. Journal of Clinical Microbiology. 51(2):508-517.
Recombinant Newcastle disease vaccines: risk for reversion to virulence and spread in non-target species -
Krishna, H., Diel, D.G., Afonso, C.L., Suarez, D.L., Miller, P.J. 2013. Recombinant Newcastle disease vaccines: risk for reversion to virulence and spread in non-target species. International Poultry Forum Proceedings. CDROM.
Genetic diversity of avian paramyxovirus type 1: Proposal for a unified nomenclature and classification system of Newcastle disease virus genotypes Reprint Icon -
Diel, D.G., Da Silva, L.A., Liu, H., Wang, Z., Miller, P.J., Afonso, C.L. 2012. Genetic diversity of avian paramyxovirus type 1: Proposal for a unified nomenclature and classification system of Newcastle disease virus genotypes. Infection, Genetics and Evolution. 12(8):1770-1779.
Experimental co-infection studies with avian influenza viruses and Newcastle Disease viruses in chickens, turkeys and domestic ducks -
Pantin Jackwood, M.J., Miller, P.J., Afonso, C.L., Spackman, E., Kapczynski, D.R., Shepherd, E.M., Smith, D.M., Cha, R., Sa E Silva, M., Swayne, D.E. 2012. Experimental co-infection studies with avian influenza viruses and Newcastle Disease viruses in chickens, turkeys and domestic ducks [abstract]. American Association of Veterinary Laboratory Diagnosticians. p.139.
Complete genome sequencing of a novel Newcastle disease virus isolate circulating in layer chickens in the Dominican Republic Reprint Icon -
Courtney, S.C., Gomez, D., Susta, L., Hines, N., Pedersen, J.C., Miller, P.J., Afonso, C.L. 2012. Complete genome sequencing of a novel Newcastle disease virus isolate circulating in layer chickens in the Dominican Republic. Journal of Virology. 86(17):9550.
Effect of age on pathogenesis and innate immune responses in Pekin ducks infected with different H5N1 highly pathogenic avian influenza viruses -
Pantin Jackwood, M.J., Smith, D.M., Wasilenko, J.L., Cagle, C.A., Shepherd, E.M., Sarmento, L., Kapczynski, D.R., Afonso, C.L. 2012. Effect of age on pathogenesis and innate immune responses in Pekin ducks infected with different H5N1 highly pathogenic avian influenza viruses. Virus Research. 167:196-206.
Experimental clinical and pathologic characterization of West African Newcastle disease viruses -
Jones, M.E., Cattoli, G., Cardenas-Garcia, S., Miller, P.J., Afonso, C.L., Brown, C.C. 2012. Experimental clinical and pathologic characterization of West African Newcastle disease viruses [abstract]. American Association of Avian Pathologists. p.22.
Characterization of the live LaSota-vaccine strain-induced protection in chickens upon early challenge with a virulent Newcastle disease virus of heterologous genotype -
Edwards, I.C., Miller, P.J., Afonso, C.L. 2012. Characterization of the live LaSota-vaccine strain-induced protection in chickens upon early challenge with a virulent Newcastle disease virus of heterologous genotype. Avian Diseases. 56:464-470.
Genetic diversity of avian paramyxovirus type 1: defining Newcastle disease virus genotypes -
Diel, D.G., Da Silva, L.H., Miller, P.J., Afonso, C.L. 2012. Genetic diversity of avian paramyxovirus type 1: defining Newcastle disease virus genotypes [abstract]. American Association of Avian Pathologists. p.22.
Biological and phylogenetic characterization of a genotype VII Newcastle disease virus from Venezuela: Efficacy of vaccination -
Perozo, F., Marcano, R., Afonso, C.L. 2012. Biological and phylogenetic characterization of a genotype VII Newcastle disease virus from Venezuela: Efficacy of vaccination. Journal of Clinical Microbiology. 50:1204-1208.
Analysis of the genome of the sexually transmitted insect virus Hz-2V -
Burand, J.P., Kim, W., Afonso, C.L., Tulman, E., Kutish, G.F., Rock, D.L. 2012. Analysis of the genome of the sexually transmitted insect virus Hz-2V. Viruses. 4(1):28-61.
Experimental characterization of West African Newcastle disease virus -
Brown, C.C., Jones, M.E., Cattoli, G., Cardenas-Garcia, S., Miller, P.J., Afonso, C.L. 2011. Experimental characterization of West African Newcastle disease virus [abstract]. RESOLAB Annual Directors Meeting. p. 1.
Characterization of Newcastle disease viruses isolated from cormorant and gull species in the United States in 2010 Reprint Icon -
Diel, D.G., Miller, P.J., Wolf, P.C., Mickley, R.M., Musante, A.R., Emanueli, D.C., Shively, K.J., Pedersen, K., Afonso, C.L. 2012. Characterization of Newcastle disease viruses isolated from cormorant and gull species in the United States in 2010. Avian Diseases. 56:128-133.
Complete genome and clinicopathological characterization of a virulent Newcastle disease virus isolate from South America Reprint Icon -
Diel, D.G., Susta, L., Cardenas Garcia, S., Killian, M.L., Brown, C.C., Miller, P.J., Afonso, C.L. 2012. Complete genome and clinicopathological characterization of a virulent Newcastle disease virus isolate from South America. Journal of Clinical Microbiology. 50(2):378-387. DOI: 10.1128/JCM.06018-11.
Comparative full genome analysis of four infectious laryngotracheitis virus (gallid herpesvirus-1) virulent isolates from the United States -
Spatz, S.J., Keeler, C.L., Kutish, G.F., Riblet, S.M., Volkening, J.D., Zsak, L., Afonso, C.L., Mundt, E.S., Rock, D.L., Garcia, M. 2012. Comparative full genome analysis of four infectious laryngotracheitis virus (gallid herpesvirus-1) virulent isolates from the United States. Virus Genes. 44:273-85.
Exchange of Newcastle disease virus fusion and hemagglutinin-neuraminidase genes into a vaccine backbone: effects on virulence -
Cardenas Garcia, S., Susta, L., Diel, D.G., Decanini, E.L., Absalon, A., Brown, C., Yu, Q., Miller, P.J., Afonso, C.L. 2012. Exchange of Newcastle disease virus fusion and hemagglutinin-neuraminidase genes into a vaccine backbone: effects on virulence . Meeting Abstract. 50:378-387.
Complete genome sequence and clinicopathological characterization of a virulent Newcastle disease virus isolated from poultry in South America -
Diel, D.G., Susta, L., Cardenas Garcia, S., Killian, M., Brown, C., Miller, P.J., Afonso, C.L. 2012. Complete genome sequence and clinicopathological characterization of a virulent Newcastle disease virus isolated from poultry in South America. Meeting Abstract. Vol.50, pages 378-387.
Bioinformatic analysis of variability of Newcastle disease virus diagnostic primers and probes and the potential for false negative detection -
Suarez, D.L., Afonso, C.L. 2011. Bioinformatic analysis of variability of Newcastle disease virus diagnostic primers and probes and the potential for false negative detection [abstract]. 1st International Respiratory Disease Converence, May 15-18, 2011, Athens, Georgia. p. 13.
Newcastle disease virus -
Miller, P.J., Afonso, C.L. 2011. Newcastle disease virus. Encyclopedia of Life Sciences. In: eLS, 2010, John Wiley & Sons, Ltd: Chichester. Available: http://www.els.net. DOI: 10.1002/9780470015902.a0001077.pub3.
Neurovirulent pathotype of Newcastle disease virus associated with the reduced capacity of NDV to replicate in vivo and in vitro -
Susta, L., Miller, P.J., Hu, S., Liu, X., Rue, C.A., Afonso, C.L., Brown, C.C. 2011. Neurovirulent pathotype of Newcastle disease virus associated with the reduced capacity of NDV to replicate in vivo and in vitro [abstract]. 1st International Avian Respiratory Disease Converence, May 15-18, 2011, Athens, Georgia. p. 58.
The effect of IL-2 expression by recombinant Newcastle disease virus on host immune response, viral replication and pathogenesis -
Edwards, I.C., Susta, L., Cardenas, S., Miller, P.J., Brown, C.C., Afonso, C.L. 2011. The effect of IL-2 expression by recombinant Newcastle disease virus on host immune response, viral replication and pathogenesis [abstract]. 1st International Avian Respiratory Disease Conference, May 15-18, 2011, Athens, Georgia. p. 34.
Construction of recombinant Newcastle disease viruses expessing chicken IFN-gamma: effect of elevated levels of expression on the transcriptional host response, viral replication and pathogenesis -
Susta, L., Edwards, I.C., Cardenas, S., Miller, P.J., Brown, C.C., Afonso, C.L. 2011. Construction of recombinant Newcastle disease viruses expessing chicken IFN-gamma: effect of elevated levels of expression on the transcriptional host response, viral replication and pathogenesis [abstract]. 1st International Avian Respiratory Disease Conference, May 15-18, 2011, Athens, Georgia. p. 15.
Virulent Newcastle disease viruses isolated from cormorant and gull species in the states of the Atlantic Flyway in 2010 -
Miller, P.J., Wolf, P.C., Mickley, R.M., Mustante, A.R., Emanueli, D.C., Shively, K.J., Afonso, C.L. 2011. Virulent Newcastle disease viruses isolated from cormorant and gull species in the states of the Atlantic Flyway in 2010 [abstract]. 1st International Avian Respiratory Disease Conference, May 15-18, 2011, Athens, Georgia. p. 54.
In vivo transcriptional cytokine responses and association with clinical and pathological outcomes in chickens infected with different Newcastle disease virus isolates using formalin-fixed paraffin-embedded samples Reprint Icon -
Ecco, R., Brown, C.C., Susta, L., Cagle, C.A., Edwards, I.C., Pantin Jackwood, M.J., Miller, P.J., Afonso, C.L. 2011. In vivo transcriptional cytokine responses and association with clinical and pathological outcomes in chickens infected with different Newcastle disease virus isolates using formalin-fixed paraffin-embedded samples. Veterinary Immunology and Immunopathology. 141:221-229.
Early occurrence of apoptosis in lymphoid tissues from chickens infected with strains of Newcastle disease virus of varying virulence Reprint Icon -
Harrison, L., Brown, C.C., Afonso, C.L., Zhang, J., Susta, L. 2011. Early occurrence of apoptosis in lymphoid tissues from chickens infected with strains of Newcastle disease virus of varying virulence. Journal of Comparative Pathology. 145:327-335.
Avian paramyxovirus serotype 1 strains of low virulence with unusual fusion protein cleavage sites isolated from poultry species -
Miller, P.J., Killian, M.L., Pederson, J.C., Afonso, C.L. 2011. Avian paramyxovirus serotype 1 strains of low virulence with unusual fusion protein cleavage sites isolated from poultry species [abstract]. American Association of Avian Pathologists Annual Meeting, July 16-19, 2011, St. Louis, Missouri. p. 114.
Analysis of transcriptional responses of chickens infected with different Newcastle disease virus isolates using paraffin embedded samples -
Ecco, R., Brown, C.C., Edwards, I.C., Cagle, C.A., Miller, P.J., Susta, L., Pantin Jackwood, M.J., Afonso, C.L. 2011. Analysis of transcriptional responses of chickens infected with different Newcastle disease virus isolates using paraffin embedded samples [abstract]. American Association of Avian Pathologists Annual Meeting, July 16-19, 2011, St. Louis, Missouri. p. 14.
Virulent Newcastle disease virus elicits a strong innate immune response in chickens -
Rue, C.A., Susta, L., Edwards, I.C., Brown, C.C., Kapczynski, D.R., Suarez, D.L., King, D.J., Miller, P.J., Afonso, C.L. 2011. Virulent Newcastle disease virus elicits a strong innate immune response in chickens. Journal of General Virology. 92:931-939.
Update on poultry viral diseases research conducted at Southeast Poultry Research Laboratory -
Pantin Jackwood, M.J., Swayne, D.E., Spackman, E., Suarez, D.L., Afonso, C.L., Miller, P.J., Zsak, L., Day, J.M., Yu, Q. 2011. Update on poultry viral diseases research conducted at Southeast Poultry Research Laboratory [abstract]. Proceedings of the United States Animal Health Association 114th Annual Meeting, November 17, 2010, Minneapolis, MN. p. 526-527.
Neurological lesions in chickens experimentally infected with virulent Newcastle disease virus isolates Reprint Icon -
Ecco, R., Susta, L., Afonso, C.L., Miller, P.J., Brown, C.C. 2011. Neurological lesions in chickens experimentally infected with virulent Newcastle disease virus isolates. Avian Pathology. 40(2):145-152.
Comparative nucleotide sequence analysis of three virulent strains of infectious laryngotracheitis virus -
Spatz, S.J., Afonso, C.L., Zsak, L., Garcia, M., Mundt, E., Riblet, S., Keeler, C., Rock, D. 2010. Comparative nucleotide sequence analysis of three virulent strains of infectious laryngotracheitis virus. Workshop on Molecular Pathogenesis of Marek's Disease and Avian Immunology. 1(1): 59.
Evidence for a new avian paramyxovirus serotype-10 detected in Rockhopper penguins from the Falkland Islands Reprint Icon -
Miller, P.J., Afonso, C.L., Spackman, E., Scott, M.A., Pedersen, J.C., Senne, D.A., Brown, J.D., Fuller, C.M., Uhart, M.M., Karesh, W.B., Brown, I.H., Alexander, D.J., Swayne, D.E. 2010. Evidence for a new avian paramyxovirus serotype-10 detected in Rockhopper penguins from the Falkland Islands. Journal of Virology. 84(21):11496-11504.
Comparative nucleotide sequence analysis of three virulent strains of infectious laryngotracheitis virus (ILTV) -
Pathogenicity evaluation of different Newcastle disease virus chimeras in 4-week-old chickens Reprint Icon -
Susta, L., Miller, P.J., Afonso, C.L., Estevez, C., Yu, Q., Zhang, J., Brown, C.C. 2010. Pathogenicity evaluation of different Newcastle disease virus chimeras in 4-week-old chickens. Tropical Animal Health and Production. 42(8):1785-1795.
Clinicopathological characterization in poultry of three strains of Newcastle disease viruses isolated from recent outbreaks Reprint Icon -
Susta, L., Miller, P.J., Afonso, C.L., Brown, C.C. 2011. Clinicopathological characterization in poultry of three strains of Newcastle disease viruses isolated from recent outbreaks. Veterinary Pathology. 48(2):349-360.
Phylogenetic and biological characterization of Newcastle disease virus isolates from Pakistan Reprint Icon -
Khan, T.A., Rue, C.A., Rehmani, S.F., Ahmed, A., Wasilenko, J.L., Miller, P.J., Afonso, C.L. 2010. Phylogenetic and biological characterization of Newcastle disease virus isolates from Pakistan. Journal of Clinical Microbiology. 48(5):1892-1894. DOI: 10.1128/JCM.00148-10.
Neurological lesions in chickens experimentally infected with virulent Newcastle disease virus isolates -
Ecco, R., Susta, L., Afonso, C.L., Miller, P.J., Brown, C.C. 2010. Neurological lesions in chickens experimentally infected with virulent Newcastle disease virus isolates [abstract]. In: Proceedings of the American Association of Avian Pathologists annual meeting, July 31-August 04, 2010, Atlanta, Georgia. Paper #9393.
Evolutionary changes affecting rapid identification of 2008 Newcastle disease viruses isolated from double-crested cormorants Reprint Icon -
Rue, C.A., Susta, L., Brown, C.C., Pasick, J.M., Swafford, S.R., Wolf, P.C., Killian, M.L., Pedersen, J.C., Miller, P.J., Afonso, C.L. 2010. Evolutionary changes affecting rapid identification of 2008 Newcastle disease viruses isolated from double-crested cormorants. Journal of Clinical Microbiology. 48(7):2440-2448. DOI: 10.1128/JCM.02213-09.
Presence of apoptosis, as determined by immunohistochemistry, in lymphoid tissues of chickens infected with strains of Newcastle disease virus of varying virulence -
Susta, L., Harrison, L., Zhang, J., Afonso, C.L., Miller, P.J., Brown, C.C. 2009. Presence of apoptosis, as determined by immunohistochemistry, in lymphoid tissues of chickens infected with strains of Newcastle disease virus of varying virulence [abstract]. American Association of Avian Pathologists/American Veterinary Medical Association Annual Meeting, July 11-15, 2009, Seattle, Washington. p. 16.
Pathological and immunohistochemical study of the reproductive tracts of vaccinated layers infected with the virulent CA02 strain of Newcastle disease virus -
Susta, L., Miller, P.J., Decanini, E.L., Absalon, A.E., Afonso, C.L., Brown, C.C. 2010. Pathological and immunohistochemical study of the reproductive tracts of vaccinated layers infected with the virulent CA02 strain of Newcastle disease virus [abstract]. In: Proceedings of the American Association of Avian Pathologists annual meeting, July 31-August 04, 2010, Atlanta, Georgia. Paper #9394.
Clinicopathological characterization of two recombinant Newcastle disease viruses derived from a virulent Chinese strain -
Susta, L., Miller, P.J., Hu, S., Liu, X., Rue, C.A., Afonso, C.L., Brown, C.C. 2010. Clinicopathological characterization of two recombinant Newcastle disease viruses derived from a virulent Chinese strain [abstract}. In: Proceedings of the American Association of Avian Pathologists annual meeting, July 31-August 04, 2010, Atlanta, Georgia. Paper #9392.
Levels of humoral antibodies induced by different inactivated vaccines correlate with egg production in commercial layers challenged with virulent Newcastle disease virus -
Miller, P.J., Susta, L., Brown, C.C., Decanini, E.L., Absalon, A.E., Afonso, C.L. 2010. Levels of humoral antibodies induced by different inactivated vaccines correlate with egg production in commercial layers challenged with virulent Newcastle disease virus [abstract]. In: Proceedings of the American Association of Avian Pathologists Annual Meeting, July 31-August 04, 2010, Atlanta, Georgia. paper #9300.
Newcastle disease: New challenges in diagnostic and vaccination -
Pantin Jackwood, M.J., Afonso, C.L., Miller, P.J. 2009. Newcastle disease: New challenges in diagnostic and vaccination [abstract]. 44th United States-Japan Cooperative Program in Natural Resources, November 30-December 5, 2009, Tsukuba, Japan. p. 33.
Research update from Southeast Poultry Research Laboratory -
Suarez, D.L., Swayne, D.E., Pantin Jackwood, M.J., Afonso, C.L., Miller, P.J., Kapczynski, D.R. 2010. Research update from Southeast Poultry Research Laboratory [abstract]. Report of the 113th United States Animal Health Association meeting, October 13, 2009, San Diego, California. p. 26-27.
Newcastle disease: Evolution of genotypes and the related diagnostic challenges Reprint Icon -
Miller, P.J., Decanini, E.L., Afonso, C.L. 2010. Newcastle disease: Evolution of genotypes and the related diagnostic challenges. Infection, Genetics and Evolution. 10(1):26-35.
Evolutionary dynamics of Newcastle disease virus Reprint Icon -
Miller, P.J., Kim, L.M., Afonso, C.L., Ip, H.S. 2009. Evolutionary dynamics of Newcastle disease virus. Virology. 391:64-72.
Pathogenesis study of selected velogenic strains of Newcastle disease virus in White Leghorn chickens -
Susta, L., Miller, P.J., Hu, S., Liu, Z., Rue, C.A., Afonso, C.L., Brown, C.C. 2009. Pathogenesis study of selected velogenic strains of Newcastle disease virus in White Leghorn chickens [abstract]. In: Proceedings of the 14th International Symposium for the World Association of Veterinary Laboratory Diagnosticians, WAVLD 2009, June 17-20, 2009, Madrid, Spain. p. 222.
Role of immune-related host gene responses in the pathobiology of H5N1 highly pathogenic avian influenza in ducks -
Pantin Jackwood, M.J., Kapczynski, D.R., Sarmento, L., Wasilenko, J.L., Afonso, C.L. 2009. Role of immune-related host gene responses in the pathobiology of H5N1 highly pathogenic avian influenza in ducks [abstract]. 7th International Symposium on Avian Influenza, April 5-9, 2009, Athens, Georgia. p. 43.
A highly virulent strain of Newcastle disease virus elicits a strong innate response and nitric oxide production in chickens -
Rue, C.A., Susta, L., Brown, C.C., Kapczynski, D.R., Miller, P.J., King, D.J., Suarez, D.L., Afonso, C.L. 2009. A highly virulent strain of Newcastle disease virus elicits a strong innate response and nitric oxide production in chickens [abstract]. American Association of Avian Pathologists Annual Meeting, July 11-14, 2009, Seattle, Washington. p. 16.
Transmission of virulent Newcastle disease virus (NDV) between unvaccinated, sub-optimally vaccinated, and well-vaccinated SPF chickens -
Miller, P.J., Afonso, C.L. 2009. Transmission of virulent Newcastle disease virus (NDV) between unvaccinated, sub-optimally vaccinated, and well-vaccinated SPF chickens [abstract]. American Association of Avian Pathologists/American Veterinary Medical Association annual meeting, July 11-15, 2009, Seattle, Washington. Paper #7606.
Use of genomic interspecies microarray hybridization to detect differentially expressed genes associated with H5N1 avian influenza virus infections in ducks -
Pantin Jackwood, M.J., Sarmento, L., Afonso, C.L. 2008. Use of genomic interspecies microarray hybridization to detect differentially expressed genes associated with H5N1 avian influenza virus infections in ducks [abstract]. Proceedings of the American Association of Veterinary Laboratory Diagnosticians Annual Meeting, October 23-29, 2008, Greensboro, NC. 2008 CDROM.
Biological and phylogenetic characterization of pigeon paramyxovirus serotype-1 circulating in North American pigeons Reprint Icon -
Kim, L.M., King, D.J., Guzman, H., Tesh, R.B., Bueno, R., Dennett, J.A., Afonso, C.L. 2008. Biological and phylogenetic characterization of pigeon paramyxovirus serotype-1 circulating in North American pigeons and doves. Journal of Clinical Microbiology. 46(10):3303-3310.
Not so fast on recombination analysis of Newcastle disease virus -
Afonso, C.L. 2008. Not so fast on recombination analysis of Newcastle disease virus. Journal of Virology. 82(18):9303.
Full genome sequencing of the Newcastle disease viruses VS/GA and clone 5 Reprint Icon -
Perozo, F., Villegas, P., Afonso, C.L. 2008. Genomic comparison of the complete coding and intergenic regions of the VG/GA Newcastle disease virus and its respirotropic clone 5. Virus Genes. 37(2):161-167.
Highly pathogenic H5N1 avian influenza viruses differentially affect gene expression in primary chicken embryo fibroblasts -
Sarmento, L., Afonso, C.L., Estevez, C., Wasilenko, J.L., Pantin Jackwood, M.J. 2008. Differential host gene expression in cells infected with highly pathogenic H5N1 avian influenza viruses. Veterinary Immunology and Immunopathology. 125:291-302.
Biological and phylogenic characterization of virulent Newcastle disease virus circulating in Mexico -
Perozo, F., Merino, R., Afonso, C.L., Villegas, P., Calderon, N. 2008. Biological and phylogenic characterization of virulent Newcastle disease virus circulating in Mexico. Avian Diseases. 52(3):472-479.
Microarray analysis following infection with highly pathogenic avian influenza H5N1 virus in naive and vaccinated SPF chickens -
Kapczynski, D.R., Afonso, C.L., Pantin Jackwood, M.J., Liljebjelke, K.A., Sarmento, L., Suarez, D.L., Swayne, D.E. 2008. Microarray analysis following infection with highly pathogenic avian influenza H5N1 virus in naive and vaccinated SPF chickens [abstract]. In: Proceedings of Avian Immunology Research Group Conference, June 24-27, 2008, Queensland, Australia. p. 22.
Detection of a broad range of class I and II Newcastle disease viruses using a multiplex real time RT-PCR assay -
Kim, L.M., Suarez, D.L., Afonso, C.L. 2008. Detection of a broad range of class I and II Newcastle disease viruses using a multiplex real-time reverse transcription polymerase chain reaction assay. Journal of Veterinary Diagnostic Investigation. 20:414-425.
The VG/GA strain of Newcastle disease virus: Mucosal immunity, protection against lethal challenge and molecular analysis -
Perozo, F., Villegas, P., Dolz, R., Afonso, C.L., Purvis, L.B. 2008. The VG/GA strain of Newcastle disease virus: Mucosal immunity, protection against lethal challenge and molecular analysis. Avian Pathology. 37(3):237-245.
Phylogenetic and biological characterization of virulent Newcastle disease viruses isolated in wild birds during 2002-2007 -
Afonso, C.L., King, D.J., Kim, L.M., Guzman, H., Tesh, R.B., Dennett, J.A. 2000. Phylogenetic and biological characterization of virulent Newcastle disease viruses isolated in wild birds during 2002-2007 [abstract]. In: Proceedings of the American Association of Avian Pathologists meeting, July 19-23, 2008, New Orleans, Louisiana. p. 118.
Gene expression responses to highly pathogenic avian influenza H5N1 virus infections in ducks -
Pantin Jackwood, M.J., Kapczynski, D.R., Wasilenko, J.L., Sarmento, L., Afonso, C.L. 2008. Gene expression responses to highly pathogenic avian influenza H5N1 virus infections in ducks [abstract]. 145th American Medical Association Annual Meeting, July 20-23, 2008, New Orleans, Louisiana. 2008 CDROM.
Viral genome sequencing bt random priming methods -
Djikeng, A., Halpin, R., Kuzmickas, R., Sengamalay, N., Afonso, C.L., Xang, X., Anderson, N.G., Ghedin, E., Spiro, D.J. 2008. Viral genome sequencing by random priming methods. Biomed Central (BMC) Genomics. 9:5.
Immediate early responses of avian tracheal epithelial cells to infection with highly pathogenic avian invluenza virus -
Sarmento, L., Pantin Jackwood, M.J., Kapczynski, D.R., Swayne, D.E., Afonso, C.L. 2008. Immediate early responses of avian tracheal epithelial cells to infection with highly pathogenic avian invluenza virus. Biologicals. 132:175-183.
Baculoviruses: Molecular Biology of Mosquito Baculoviruses -
Becnel, J.J., Afonso, C.L. 2008. Baculoviruses: Molecular Biology of Mosquito Baculoviruses. Encyclopedia of virology, 5 vols. (B.W.J. Mahy and M.H.V. Van Regenmortel, Editors), pp. 219-225 Oxford:Elsevier.
Characterization and phylogenic analysis of Mexican Newcastle disease virus isolates -
Merino, R., Calderon, N., Perozo, F., Villegas, P., Afonso, C.L. 2008. Characterization and phylogenic analysis of Mexican Newcastle disease virus isolates [abstract]. International Poultry Scientific Forum, January 21-22, 2008, Atlanta, Georgia. p. 33.
Variable regulation of chicken embryo fibroblasts gene expression by H5N1 avian influenza viruses -
Sarmento, L., Pantin Jackwood, M.J., Afonso, C.L., Wasilenko, J.L., Estevez, C. 2008. Variable regulation of chicken embryo fibroblasts gene expression by H5N1 avian influenza viruses [abstract]. International Poultry Scientific Forum, January 21-22, 2008, Atlanta, Georgia. p. 33.
Primer sequence-independent full genome amplification and sequencing of the VG/GA strain of Newcastle disease virus -
Perozo, F., Villegas, P., Afonso, C.L. 2008. Primer sequence-independent full genome amplification and sequencing of the VG/GA strain of Newcastle disease virus [abstract]. International Poultry Scientific Forum, January 21-22, 2008, Atlanta, Georgia. p. 32.
Research update on exotic and emerging poultry diseases -
Pantin Jackwood, M.J., Suarez, D.L., Zsak, L., Spackman, E., Kapczynski, D.R., King, D.J., Afonso, C.L., Day, J.M., Spatz, S.J., Yu, Q., Swayne, D.E. 2007. Research update on exotic and emerging poultry diseases. In: Proceedings of the 111th Annual Meeting of the U. S. Animal Health Association, Reno, Nevada, October 22-23, 2007. p. 702-707. Available: http://www.usaha.org/meetings/2007/2007_USAHA_Proceedings.pdf.
A primary chicken tracheal cell culture system for the study of infection with avian respiratory viruses -
Zaffuto, K.M., Estevez, C., Afonso, C.L. 2008. Primary chicken tracheal cell culture system for the study of infection with avian respiratory viruses. Avian Pathology. 37(1):25-31.
Phylogenetic diversity among low virulence Newcastle disease viruses from waterfowl and shorebirds and comparison of genotype distributions to poultry-origin isolates -
Kim, L.M., King, D.J., Curry, P., Suarez, D.L., Swayne, D.E., Stallknecht, D., Slemons, R.D., Pedersen, J.C., Senne, D.S., Winker, K., Afonso, C.L. 2007. Phylogenetic diversity among low-virulence Newcastle disease viruses from waterfowl and shorebirds and comparison of genotype distributions to those of poultry-origin isolates. Journal of Virology. 81(22):12641-12653.
Sheeppox virus kelch-like gene SPPV-019 affects virus virulence -
Balinsky, C.A., Delhon, G., Afonso, C.L., Risatti, G.R., Borca, M.V., French, R.A., Tulman, E.R., Geary, S.J., Rock, D.L. 2007. Sheeppox virus kelch-like gene SPPV-019 affects virus virulence. Journal of Virology. 81(20):11392-11401.
Antigenic differences among NDV strains of different genotypes used in vaccine formulation affects viral shedding after a virulent challenge -
Miller, P.J., King, D.J., Afonso, C.L., Suarez, D.L. 2007. Antigenic differences among NDV strains of different genotypes used in vaccine formulation affects viral shedding after a virulent challenge. Vaccine. 25:7238-7246.
Classical Swine Fever Virus Inhibits Nitric Oxide Production in Infected Macrophages -
Zaffuto, K.M., Piccone, M.E., Burrage, T.G., Risatti, G.R., Borca, M.V., Holinka, L.G., Rock, D.L., Afonso, C.L. 2007. Classical Swine Fever Virus Inhibits Nitric Oxide Production in Infected Macrophages. Journal of General Virology. 88:3007-3012.
Early responses of chicken lungs and spleens to infection with highly pathogenic avian influenza virus using microarray analysis -
Afonso, C.L., Kapczynski, D.R., Pantin Jackwood, M.J., Sarmento, L., Swayne, D.E. 2007. Early responses of chicken lungs and spleens to infection with highly pathogenic avian influenza virus using microarray analysis [abstract]. In: Proceedings of the International Symposium on Animal Genomics for Animal Health, October 23-25, 2007, Paris France. p. 59.
Immediate early responses of avian tracheal epithelial cells to infection with highly pathogenic avian influenza -
Afonso, C.L., Sarmento, L., Pantin Jackwood, M.J., Kapczynski, D.R., Swayne, D.E. 2007. Immediate early responses of avian tracheal epithelial cells to infection with highly pathogenic avian influenza [abstract]. In: Proceedings of the International Symposium on Animal Genomics for Animal Health, October 23-25, 2007, Paris, France. p. 16.
Detection and isolation of exotic Newcastle disease (ENDV) from field collected flies -
Chakrabarti, S., King, D.J., Afonso, C.L., Swayne, D.E., Cardona, C.J., Kuney, D.R., Gerry, A.C. 2007. Detection and isolation of exotic Newcastle disease virus from field-collected flies. Journal of Medical Entomology. 44(5):840-844.
Design of a multiplex real time RT-PCR assay to detect Newcastle disease viruses from classes I and II -
Kim, L.M., Suarez, D.L., Afonso, C.L. 2007. Design of a multiplex real time RT-PCR assay to detect Newcastle disease viruses from classes I and II [abstract]. 10th International Conference for Biodetection Technologies 2007, June 14-15, 2007, Atlanta, Georgia. Poster #8.
Sequencing of avian influenza virus genomes following random amplification -
Afonso, C.L. 2007. Sequencing of avian influenza virus genomes following random amplification. Biotechniques. 43(2):188-192.
Isolation and genetic characterization of influenza A viruses and a paramyxovirus from wild birds in Barbados: 2003-2004 -
Douglas, K.O., Lavoie, M.L., Suarez, D.L., Afonso, C.L., Kim, L.M. 2007. Isolation and genetic characterization of avian influenza viruses and a Newcastle disease virus from wild birds in Barbados: 2003-2004. Avian Diseases. 51(3):781-787.
Isolation of exotic Newcastle disease virus (ENDV) from field collected flies and experimental ENDV infections of three arthropod species -
King, D.J., Chakrabarti, S., Cardona, C., Afonso, C.L., Swayne, D.E., Gerry, A.C. 2007. Isolation of exotic Newcastle disease virus (ENDV) from field collected flies and experimental ENDV infections of three arthropod species [abstract]. American Association of Avian Pathologists/American Veterinary Medical Association Meeting Program, July 14-18, 2007, Washington, DC. p. 44.
Characterization of Class I Newcastle disease virus isolates from Hong Kong bird markets and detection using real-time reverse transcription PCR -
Kim, L.M., King, D.J., Suarez, D.L., Wong, C.W., Afonso, C.L. 2007. Characterization of Class I Newcastle disease virus isolates from Hong Kong bird markets and detection using real-time reverse transcription PCR. Journal of Clinical Microbiology. 45:1310-1314.
Newcastle disease virus isolates from US waterfowl reveal novel genomic subgroups with diverse and evolving genetics and the potential for spillover into US live bird markets -
Kim, L.M., King, D.J., Swayne, D.E., Stallknecht, D., Slemons, R.D., Pedersen, J.C., Senne, D.A., Winker, K., Afonso, C.L. 2007. Newcastle disease virus isolates from US waterfowl reveal novel genomic subgroups with diverse and evolving genetics and the potential for spillover into US live bird markets [abstract]. In: Abstracts of the American Association of Avian Pathologists meeting, July 14-19, 2007, Washington, DC. p. 43. CD.
Development and characterization of a primary culture of chicken embryonic tracheal epithelial cells and their use in avian studies -
Zaffuto, K.M., Suarez, D.L., Estevez, C., Afonso, C.L. 2007. Development and characterization of a primary culture of chicken embryonic tracheal epithelial cells and their use in avian studies [abstract]. American Association of Avian Pathologists Annual Meeting, July 14-19, 2007, Washington, DC. p. 17.
Differential growth of avian influenza virus in chicken and duck cells -
Sarmento, L., Zaffuto, K.M., Pantin Jackwood, M.J., Afonso, C.L. 2007. Differential growth of avian influenza virus in chicken and duck cells [abstract]. 144th American Veterinary Medical Association Annual Meeting, July 14-19, 2007, Washington, DC. CD-ROM.
Why are avian influenza viruses emerging and what tools are needed to prevent and control the infection and disease? -
Swayne, D.E., Suarez, D.L., Pantin Jackwood, M.J., Spackman, E., Kapczynski, D.R., Afonso, C.L. 2007. Why are avian influenza viruses emerging and what tools are needed to prevent and control the infection and disease?. In: Proceedings of the 110th Annual Meeting of the U.S. Animal Health Association, October 12-18, 2006, Minneapolis, Minnesota. p. 728-729.
Use of bioinformatics in improving detection of Newcastle disease virus -
Kim, L.M., Suarez, D.L., Afonso, C.L. 2007. Use of bioinformatics in improving detection of Newcastle disease virus. In: Rogeria de Almeida, M., Pires Moraes, M., Patarroyo, J.H., Vidigal, P.M.P., Borem, A., editors. Biotecnologia e Saude Animal. Viscoza, Brazil: Federal University of Viscosa. Chapter 9. p. 227-249.
Genus Suipoxvirus -
Gustavo, D.A., Tulman, E.R., Afonso, C.L., Rock, D.L. 2007. Genus Suipoxvirus. In: Mercer, A., Schmidt, A., Weber, O. (editors). Poxviruses. Birkhauser Basel. p. 203-215.
RECOVERY OF EXOTIC NEWCASTLE DISEASE VIRUS (ENDV) FROM FIELD COLLECTED FLIES AND EXPERIMENTAL ENDV INFECTIONS OF THREE ARTHROPOD SPECIES -
Chakrabarti, S., King, D.J., Cardona, C., Afonso, C.L., Swayne, D.E., Gerry, A.C. 2006. Recovery of exotic Newcastle disease virus (ENDV) from field collected flies and experimental ENDV infections of three arthropod species [abstract]. The 54th Annual Meeting of the Entomological Society of America, December 10-13, 2006, Indianapolis, Indiana. p. 93.
GENOME OF INVERTEBRATE IRIDESCENT VIRUS 3 (MOSQUITO IRIDESCENT VIRUS) -
Delhon, G., Tulman, E., Afonso, C.L., Lu, Z., Becnel, J.J., Moser, B.A., Kutish, G.F., Rock, D.L. 2006. Genome of invertebrate iridescent virus 3 (mosquito iridescent virus). Journal of Virology. 80(17):8439-8449.
GENOME OF HORSEPOX VIRUS -
Tulman, E., Delhon, G., Afonso, C.L., Lu, Z., Zsak, L., Sandybaev, N.T., Kerembekova, U.Z., Zaitsev, V.L., Kutish, G.F., Rock, D.L. 2006. Genome of horsepox virus. Journal of Virology. 80(18):9244-9258.
POTENTIAL ROLE OF FLIES IN THE PERSISTENCE AND DISPERSAL OF EXOTIC NEWCASTLE DISEASE VIRUS IN SOUTHERN CALIFORNIA -
Chakrabarti, S., King, D.J., Cardona, C., Afonso, C.L., Swayne, D.E., Gerry, A.C. 2006. Potential role of flies in the persistence and dispersal of exotic Newcastle disease virus in Southern California [abstract]. Livestock Insect Worker's Conference Annual Meeting. p. 34.
PHYLOGENETIC CHARACTERIZATION OF ENDEMIC NEWCASTLE DISEASE VIRUSES ISOLATED FROM WILD BIRDS DURING 2000 TO 2004 IN DELAWARE, MARYLAND AND NEW JERSEY -
Afonso, C.L. 2006. Phylogenetic characterization of endemic Newcastle disease viruses isolated from wild birds during 2000 to 2004 in Delaware, Maryland and New Jersey [abstract]. American Association of Avian Pathologists 2006 Meeting, Hawaii. p. 154.
EFFECT OF PROBE-SITE MISMATCHES ON DETECTION OF VIRULENT NEWCASTLE DISEASE VIRUSES USING A FUSION-GENE REAL-TIME REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION TEST -
Kim, L.M., Afonso, C.L., Suarez, D.L. 2006. Effect of probe-site mismatches on detection of virulent Newcastle disease viruses using a fusion-gene real-time reverse transcription polymerase chain reaction test. Journal of Veterinary Diagnostic Investigation. 18:519-528.
REAL TIME RT-PCR DEVELOPED FOR A PHYLOGENETICALLY DIVERGENT GROUP OF NEWCASTLE VIRUSES NOT DETECTED BY CURRENT TESTS -
Kim, L.M., Afonso, C.L., Suarez, D.L., King, D.J. 2006. Real time RT-PCR developed for a phylogenetically divergent group of Newcastle viruses not detected by current tests [abstract]. American Association of Avian Pathologists. CD p. 156.
GENOME OF CROCODILEPOX VIRUS -
Afonso, C.L., Tulman, E.R., Delhon, G., Lu, Z., Viljoen, G.J., Wallace, D.B., Kutish, G.F., Rock, D.L. 2006. Genome of crocodilepox virus. Journal of Virology. 80(10)4978-4991.
CONTRASTING RESULTS FROM MOLECULAR AND BIOLOGICAL PATHOGENICITY ASSAYS OF NEWCASTLE DISEASE VIRUS ISOLATE IOWA 1519 -
King, D.J., Kapczynski, D.R., Afonso, C.L., Piacenti, A. 2005. Contrasting results from molecular and biological pathogenicity assays of newcastle disease virus isolate Iowa 1519 [abstract]. American Association of Avian Pathologists, p.33.
GENOME SEQUENCING AND ANALYSIS -
Afonso, C.L., Kutish, G.F. 2004. Genome sequencing and analysis [abstract]. Society of Invertebrate Pathology.
AFRICAN SWINE FEVER VIRUS MULTIGENE FAMILY 360 AND 530 GENES AFFECT HOST INTERFERON RESPONSE -
Afonso, C.L., Piccone, M.E., Zaffuto, K., Neilan, J.G., Kutish, G.F., Lu, Z., Balinsky, C.A., Gibb, T.R., Zsak, L., Rock, D.L. 2004. African swine fever virus multigene family 360 and 530 genes affect host interferon response. Journal of Virology. 78(4):1858-1864.
AFRICAN SWINE FEVER VIRUS MULTIGENE FAMILY 360 AND 530 GENES AFFECT HOST INTERFERON RESPONSE -
Afonso, C., Piccone, M.E., Zaffuto, K.M., Neilan, J.G., Kutish, G., Lu, Z., Balinsky, C.A., Gibb, T., Bean, T.R., Zsak, L., Rock, D.L. 2004. African swine fever virus multigene family 360 and 530 genes affect host interferon response.. Journal of Virology. (2004) 78 (4):1858-1864.
GENOMES OF PARAPOXVIRUSES ORF VIRUS AND BOVINE PAPULAR STOMATITIS VIRUS -
Delhon, G., Tulman, E., Afonso, C.L., Lu, Z., Concha-Bermejillo, A., Lehmkuhl, H., Piccone, M.E., Kutish, G.F., Rock, D.L. 2004. Genomes of parapoxviruses orf virus and bovine papular stomatitis virus. Journal of Virology. 78(1):168-177.
GENOMES OF THE PARAPOXVIRUSES ORF VIRUS AND BOVINE PAPULAR STOMATITIS VIRUS -
Delhon, G., Tulman, E., Afonso, C., Lu, Z., De La Concha-Bermeji, A., Lehmkuhl, H., Piccone, M.E., Kutish, G., Rock, D.L. 2004. Genomes of the parapoxviruses orf virus and bovine papular stomatitis virus.. Journal of Virology. 78(1):168-177.
THE GENOME OF CANARYPOX VIRUS -
Tulman, E., Afonso, C.L., Lu, Z., Zsak, L., Kutish, G.F., Rock, D.L. 2004. The genome of canarypox virus. Journal of Virology. 78(1):353-366.
HZ-2V GENOME ANALYSIS -
Kim, W., Burand, J.P., Afonso, C.L., Kutish, G.F., Lu, Z., Rock, D.L. 2003.Hz-2v genome analysis [abstract]. Society for Invertebrate Pathology Annual Meeting. P. 78.
GENOME OF BOVINE HERPESVIRUS 5 -
Delhon, G., Moraes, M.P., Lu, Z., Afonso, C.L., Flores, E.F., Weiblen, R., Kutish, G.F., Rock, D.L. 2003. Genome of bovine herpesvirus 5. Journal of Virology. 77(19):10339-10347.
LIGATION PROPERTIES OF A NEW CLASS OF NAD-DEPENDENT DNA LIGASE FROM ENTOMOPOXVIRUS MELANOPLUS SANGUINIPES -
A NOVEL SWINE VIRULENCE DETERMINANT IN THE LEFT VARIABLE REGION OF THE AFRICAN SWINE FEVER VIRUS GENOME -
THE GENOME OF CULEX NIGRIPALPUS BACULOVIRUS -
THE GENOME OF CULEX NIGRIPALPUS BACULOVIRUS -
ARS News Articles
Last Modified: 5/2/2016
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