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ARS Home » Midwest Area » Ames, Iowa » National Animal Disease Center » Ruminant Diseases and Immunology Research » People & Locations » Shollie Falkenberg
Shollie M Falkenberg
Ruminant Diseases and Immunology Research
Research Microbiologist

Phone: (515) 337-7338
Fax: (515) 337-7402

P.O. BOX 70
1920 DAYTON AVE
AMES, IA 50010
Projects
Identification of Disease Mechanisms and Control Strategies for Viral Respiratory Pathogens of Ruminants
In-House Appropriated (D)
  Accession Number: 432021
Intracellular Killing And Biofilm Destruction Of MDR Salmonella By NK-Lysin Encapsulated Nanoparticles
Non-Assistance Cooperative Agreement (S)
  Accession Number: 438266

Publications (Clicking on the reprint icon Reprint Icon will take you to the publication reprint.)
Experimental innoculation of young calves with SARS-CoV-2 - (Peer Reviewed Journal)
Bovine NK-lysin-derived peptides are bactericidal against Mycobacterium avium subspecies paratuberculosis Reprint Icon - (Peer Reviewed Journal)
Dassanayake, R.P., Wherry, T.L., Falkenberg, S.M., Reinhardt, T.A., Casas, E., Stabel, J.R. 2021. Bovine NK-lysin-derived peptides are bactericidal against Mycobacterium avium subspecies paratuberculosis. Veterinary Research. 52. https://doi.org/10.1186/s13567-021-00893-2.
Virus strain influenced the interspecies transmission of influenza D virus between calves and pigs - (Peer Reviewed Journal)
Experimental intravenous, intratracheal, and intranasal inoculation of swine with SARS-CoV-2 - (Abstract Only)
Buckley, A.C., Falkenberg, S.M., Laverack, M., Martins, M., Palmer, M.V., Diel, D., Lager, K.M. 2020. Experimental intravenous, intratracheal, and intranasal inoculation of swine with SARS-CoV-2 [abstract]. American Association of Swine Veterinarians Annual Meeting. p. 35-37.
Changes in circulating lymphocytes and lymphoid tissue associated with vaccination of colostrum deprived calves - (Peer Reviewed Journal)
Multivariate analysis as a method to evaluate antigenic relationships between BVDV vaccine and field strains Reprint Icon - (Peer Reviewed Journal)
Mosena, A.C., Falkenberg, S.M., Ma, H., Casas, E., Dassanayake, R.P., Watz, P., Canal, C., Neill, J.D. 2020. Multivariate analysis as a method to evaluate antigenic relationships between BVDV vaccine and field strains. Vaccine. 38(36):5764-5772. https://doi.org/10.1016/j.vaccine.2020.07.010.
Identification of a reliable fixative solution to preserve complex architecture of bacterial biofilms for scanning electron microscopy evaluation Reprint Icon - (Peer Reviewed Journal)
Dassanayake, R.P., Falkenberg, S.M., Stasko, J.A., Shircliff, A.L., Lippolis, J.D., Briggs, R.E. 2020. Identification of a reliable fixative solution to preserve complex architecture of bacterial biofilms for scanning electron microscopy evaluation. PLoS One. 15(5):e0233973. https://doi.org/10.1371/journal.pone.0233973.
Bovine viral diarrhea virus: an updated ACVIM consensus statement with focus on virus biology, hosts, immunosuppression, and vaccination Reprint Icon ()
Walz, P.H., Chamorro, M.F., Falkenberg, S.M., Passler, T., van der Meer, F., Woolums, A. 2020. Bovine viral diarrhea virus: an updated American College of Veterinary Internal Medicine consensus statement with focus on virus biology, hosts, immunosuppression, and vaccination. Journal of Veterinary Internal Medicine. 2020;1-17. https://doi.org/10.1111/jvim.15816.
Measuring CMI responses using the PrimeFlow RNA assay; a new method of evaluating BVDV vaccination response in cattle Reprint Icon - (Peer Reviewed Journal)
Falkenberg, S.M., Dassanayake, R.P., Neill, J.D., Walz, P., Casas, E., Ridpath, J.F., Roth, J. 2020. Measuring CMI responses using the PrimeFlow RNA assay; a new method of evaluating BVDV vaccination response in cattle. Veterinary Immunology and Immunopathology. 221:110024. https://doi.org/10.1016/j.vetimm.2020.110024.
Persistent atypical porcine pestivirus (APPV) infection in gilts - (Abstract Only)
Buckley, A.C., Arruda, B., Falkenberg, S.M., Miller, L.C., Lager, K.M. 2019. Persistent atypical porcine pestivirus (APPV) infection in gilts. Meeting Abstract. p. 35.
In vitro method to evaluate virus competition between BVDV-1 and BVDV-2 strains using the PrimeFlow RNA assay Reprint Icon - (Peer Reviewed Journal)
Silveria, S., Falkenberg, S.M., Dassanayake, R.P., Walz, P.H., Ridpath, J.F., Canal, C.W., Neill, J.D. 2019. In vitro method to evaluate virus competition between BVDV-1 and BVDV-2 strains using the PrimeFlow RNA assay. Virology. 536:101-109. https://doi.org/10.1016/j.virol.2019.07.029.
Genomic and antigenic characterization of a cytopathic bovine viral diarrhea virus 1i isolated in the United States Reprint Icon - (Peer Reviewed Journal)
Neill, J.D., Crossley, B.M., Mosena, A.C., Ridpath, J.F., Bayles, D.O., Killian, M.L., Falkenberg, S.M. 2019. Genomic and antigenic characterization of a cytopathic bovine viral diarrhea virus 1i isolated in the United States. Virology. 535:279-282. https://doi.org/10.1016/j.virol.2019.07.020.
Synthetic bovine NK-lysin-derived peptide (bNK2A) does not require intra-chain disulfide bonds for bactericidal activity Reprint Icon - (Peer Reviewed Journal)
Dassanayake, R.P., Falkenberg, S.M., Nicholson, E.M., Briggs, R.E., Tatum, F.M., Sharma, V.K., Reinhardt, T.A. 2019. Synthetic bovine NK-lysin-derived peptide (bNK2A) does not require intra-chain disulfide bonds for bactericidal activity. PLoS One. 14(6). https://doi.org/10.1371/journal.pone.0218507.
A serosurvey for bovine Influenza D virus exposure from cattle in the United States, 2014-2015 Reprint Icon - (Peer Reviewed Journal)
Silveira, S., Falkenberg, S.M., Kaplan, B.S., Crossley, B., Ridpath, J.F., Bauermann, F.B., Fossler, C.P., Dargatz, D.A., Dassanayake, R.P., Vincent, A.L., Canal, C.W., Neill, J.D. 2019. Serosurvey for influenza D virus exposure in cattle, United States, 2014-2015. Emerging Infectious Diseases. 25(11). https://doi.org/10.3201/eid2511.1902532.
Bovine NK-lysin-derived peptide NK2A demonstrates immunomodulatory activity on cattle leukocytes - (Abstract Only)
Impact of CD46 knockout on bovine viral diarrhea virus (BVDV) replication in Madin-Darby bovine kidney cells - (Abstract Only)
MicroRNA expression in thymus from calves in a coinfection study challenged with bovine viral diarrhea virus and Mycoplasma bovis - (Abstract Only)
Identification of BVDV2b and 2c subgenotypes in the United States: genetic and antigenic characterization Reprint Icon - (Peer Reviewed Journal)
Neill, J.D., Workman, A.M., Hesse, R., Bai, J., Poulsen-Porter, E., Meadors, B., Anderson, J., Bayles, D.O., Falkenberg, S.M. 2019. Identification of BVDV2b and 2c subgenotypes in the United States: genetic and antigenic characterization. Virology. 528:19-29. https://doi.org/10.1016/j.virol.2018.12.002.
Frequency of bovine viral diarrhea virus detected in subpopulations of peripheral blood mononuclear cells in persistently infected animals and health outcome Reprint Icon - (Peer Reviewed Journal)
Falkenberg, S.M., Dassanayake, R.P., Walz, P., Casas, E., Neill, J.D., Ridpath, J.F. 2019. Frequency of bovine viral diarrhea virus detected in subpopulations of peripheral blood mononuclear cells in persistently infected animals and health outcome. Veterinary Immunology and Immunopathology. 207:46-52. https://doi.org/10.1016/j.vetimm.2018.11.015.
Distribution of atypical porcine pestivirus in the cerebellum of newborn piglets following in utero inoculation - (Abstract Only)
Experimental infection of calves with bovine gammaherpesvirus 4 - (Abstract Only)
Relative virulence in bison and cattle of bison-associated genotypes of mycoplasma bovis Reprint Icon - (Peer Reviewed Journal)
Register, K.B., Olsen, S.C., Sacco, R.E., Ridpath, J., Falkenberg, S., Briggs, R., Kanipe, C., Madison, R. 2018. Relative virulence in bison and cattle of bison-associated genotypes of Mycoplasma bovis. Veterinary Microbiology. 222:55-63. https://doi.org/10.1016/j.vetmic.2018.06.020.
Antimicrobial activity of bovine NK-lysin-derived peptides on Mycoplasma bovis Reprint Icon - (Peer Reviewed Journal)
Dassanayake, R.P., Falkenberg, S.M., Register, K.B., Samorodnitsky, D., Nicholson, E.M., Reinhardt, T.A. 2018. Antimicrobial activity of bovine NK-lysin-derived peptides on Mycoplasma bovis. PLoS One. 13(5):e0197677. https://doi.org/10.1371/journal.pone.0197677.
Serological survey for antibodies against pestiviruses in Wyoming domestic sheep Reprint Icon - (Peer Reviewed Journal)
Silveira, S., Falkenberg, S.M., Elderbrook, M.J., Sondgeroth, K.S., Dassanayake, R.P., Neill, J.D., Ridpath, J.F., Canal, C.W. 2018. Serological survey for antibodies against pestiviruses in Wyoming domestic sheep. Veterinary Microbiology. 219:96-99. https://doi.org/10.1016/j.vetmic.2018.04.019.
Comparison of reproductive protection against bovine viral diarrhea virus provided by multivalent viral vaccines containing inactivated fractions of bovine viral diarrhea virus 1 and 2 Reprint Icon - (Peer Reviewed Journal)
Walz, P.H., Riddell, K.P., Newcomer, B.W., Neill, J.D., Falkenberg, S.M., Cortese, V.S., Scruggs, D.W., Short, T.H. 2018. Comparison of reproductive protection against bovine viral diarrhea virus provided by multivalent viral vaccines containing inactivated fractions of bovine viral diarrhea virus 1 and 2. Vaccine. 36(26):3853-3860. https://doi.org/10.1016/j.vaccine.2018.04.005.
Relationship between viral shedding in the transmission of bovine viral diarrhea virus to naïve calves by direct and indirect exposure routes Reprint Icon - (Peer Reviewed Journal)
Falkenberg, S.M., Dassanayake, R.P., Neill, J.D., Ridpath, J.F. 2018. Evaluation of bovine viral diarrhea virus transmission potential to naïve calves by direct and indirect exposure routes. Veterinary Microbiology. 217:144-148. https://doi.org/10.1016/j.vetmic.2018.03.012.
Sequential exposure to bovine viral diarrhea virus and bovine coronavirus results in increased respiratory disease lesions: clinical, immunologic, pathologic, and immunohistochemical findings Reprint Icon - (Peer Reviewed Journal)
Ridpath, J., Fulton, R.W., Bauermann, F.V., Falkenberg, S.M., Welch, J., Confer, A.W. 2020. Sequential exposure to bovine viral diarrhea virus and bovine coronavirus results in increased respiratory disease lesions: clinical, immunologic, pathologic, and immunohistochemical findings. Journal of Veterinary Diagnostic Investigation. https://doi.org/10.1177/1040638720918561.
Antimicrobial activity of bovine NK-lysin-derived peptides on Mycoplasma bovis - (Abstract Only)
Clinical report: Detection and management of bovine viral diarrhea virus Type 1b in a large dairy herd - (Peer Reviewed Journal)
Cox, E.A., Ridpath, J.F., Falkenberg, S.M. 2017. Clinical report: Detection and management of bovine viral diarrhea virus Type 1b in a large dairy herd. Bovine Practitioner Journal. 51(2):153-156.
Antimicrobial activity of bovine NK-lysin-derived peptides on bovine respiratory pathogen Histophilus somni Reprint Icon - (Peer Reviewed Journal)
Dassanayake, R.P., Falkenberg, S.M., Briggs, R.E., Tatum, F.M., Sacco, R.E. 2017. Antimicrobial activity of bovine NK-lysin-derived peptides on bovine respiratory pathogen Histophilus somni. PLoS One. 12(8):1-14. doi. 10.1371/journal.pone.0183610.
Characterization of thymus-associated lymphoid depletion in bovine calves acutely or persistently infected with bovine viral diarrhea virus 1, bovine viral diarrhea 2 or HoBi-like pestivirus Reprint Icon - (Peer Reviewed Journal)
Falkenberg, S.M., Bauermann, F.V., Ridpath, J.F. 2017. Characterization of thymus-associated lymphoid depletion in bovine calves acutely or persistently infected with bovine viral diarrhea virus 1, bovine viral diarrhea 2 or HoBi-like pestivirus. Archives of Virology. 1-8. doi. 10.1007/s00705-017-3523-x.
Improved detection of Bovine Viral Diarrhea Virus in Bovine lymphoid cell lines using PrimeFlow RNA assay Reprint Icon - (Peer Reviewed Journal)
Falkenberg, S.M., Dassanayake, R.P., Silveira, S., Neill, J.D., Ridpath, J.F. 2017. Improved detection of Bovine Viral Diarrhea Virus in Bovine lymphoid cell lines using PrimeFlow RNA assay. American Association of Veterinary Laboratory Diagnosticians. 235:77-81. 10.1016/j.virol.2017.06.032.
Serological survey for antibodies against pestiviruses in sheep in Wyoming - (Abstract Only)
Demonstration of systemic infection of BVDV Vaccine virus after vaccination in presence of PI calves - (Abstract Only)
Antimicrobial activity of bovine NK-lysin-derived peptides on bovine respiratory pathogen Histophilus somni - (Abstract Only)
Improved detection of bovine viral diarrhea virus in bovine lymphoid cell lines using PrimeFlow RNA assay Reprint Icon - (Peer Reviewed Journal)
Falkenberg, S.M., Dassanayake, R.P., Neill, J.D., Ridpath, J.F. 2017. Improved detection of bovine viral diarrhea virus in bovine lymphoid cell lines using PrimeFlow RNA assay. Virology. 509(2017):260-265. doi. 10.1016/j.virol.2017.06.032.
Isolation and characterization of a novel cervid adenovirus from white-tailed deer (Odocoileus virginianus) fawns in a captive herd Reprint Icon - (Peer Reviewed Journal)
Ridpath, J.F., Neill, J.D., Palmer, M.V., Bauermann, F.V., Falkenberg, S.M., Wolff, P.L. 2017. Isolation and characterization of a novel cervid adenovirus from white-tailed deer (Odocoileus virginianus) fawns in a captive herd. Virus Research. 238:198-203. doi: 10.1016/j.virusres.2017.06.020.
An inactivated influenza D virus vaccine partially protects cattle from respiratory disease caused by homologous challenge Reprint Icon - (Peer Reviewed Journal)
Hause, B.M., Huntimer, L., Falkenberg, S.M., Henningson, J., Lechtenberg, K., Halbur, T. 2016. An inactivated influenza D virus vaccine partially protects cattle from respiratory disease caused by homologous challenge. Veterinary Microbiology. 199(2017):47-53.
Morphological changes in cultured bovine lymphoid cell lines associated with bovine viral diarrhea virus (BVDV) single and dual infections with bovine leukemia virus (BLV) - (Abstract Only)
HoBi-like virus challenge of pregnant cows that had previously given birth to calves persistently infected with bovine viral diarrhea virus - (Peer Reviewed Journal)
Evaluation of the innate immune response of Angus heifers with genetic marker variation for intramuscular fat deposition following a lipopolysaccharide challenge - (Peer Reviewed Journal)
Buntyn, J.O., Carroll, J.A., Smith, T., Rivera, J.D., Sanchez, N.C., Broadway, P.R., Falkenberg, S.M., Schmidt, T.B. 2016. Evaluation of the innate immune response of Angus heifers with genetic marker variation for intramuscular fat deposition following a lipopolysaccharide challenge. Livestock Science. 188:103-110.
Comparison of the breadth and complexity of bovine viral diarrhea (BVDV) populations circulating in 34 persistently infected cattle generated in one outbreak Reprint Icon - (Peer Reviewed Journal)
Ridpath, J.F., Bayles, D.O., Neill, J.D., Falkenberg, S.M., Bauermann, F.F., Holler, L., Braun, L.J., Young, D.B., Kane, S.E., Chase, C.C. 2015. Comparison of the breadth and complexity of bovine viral diarrhea (BVDV) populations circulating in 34 persistently infected cattle generated in one outbreak. Virology. 485:297-304. doi. 10.1016/j.virol.2015.07.022.
Relative virulence in bison and cattle of bison-associated genotypes of Mycoplasma bovis - (Abstract Only)
Association of serum antibody levels following vaccination with a modified live BVDV vaccine and protection from clinical disease upon challenge Reprint Icon - (Peer Reviewed Journal)
Falkenberg, S.M., Ridpath, J.F., Tait Jr, R.G., Vander Ley, B., Bauermann, F.V., Reecy, J.M. 2015. Association of serum antibody levels following vaccination with a modified live BVDV vaccine and protection from clinical disease upon challenge. Jacobs Journal of Vaccines and Vaccination. 1(1):002.
Changes observed in the thymus and lymph nodes 14 days after exposure to BVDV field strains of enhanced or typical virulence in neonatal calves Reprint Icon - (Peer Reviewed Journal)
Falkenberg, S.M., Johnson, C., Bauermann, F.V., McGill, J.L., Palmer, M.V., Sacco, R.E., Ridpath, J.F. 2014. Changes observed in the thymus and lymph nodes 14 days after exposure to BVDV field strains of enhanced or typical virulence in neonatal calves. Veterinary Immunology and Immunopathology. 160(1-2):70-80. DOI: 10.1016/j.vetimm.2014.03.018.
Comparison of temperature fluctuations at multiple anatomical locations in cattle during exposure to bovine viral diarrhea virus Reprint Icon - (Peer Reviewed Journal)
Falkenberg, S.M., Ridpath, J.F., Vander Ley, B., Bauermann, F.V., Carroll, J.A. 2014. Comparison of temperature fluctuations at multiple anatomical locations in cattle during exposure to bovine viral diarrhea virus. Livestock Science. 164(2014):159-167. DOI:10.1016/j.livsci.2014.03.018.
Lack of evidence for the presence of emerging HoBi-like viruses in North American fetal bovine serum lots. Reprint Icon - (Peer Reviewed Journal)
Bauermann, F.V., Flores, E.F., Falkenberg, S.M., Ridpath, J.F. 2014. Lack of evidence for the presence of emerging HoBi-like viruses in North American fetal bovine serum lots.. Journal of Veterinary Diagnostic Investigation. 26(1):10-17. DOI: 10.1177/1040638713518208.
Evaluation of endocrine and immune disruption of infectious bovine rhinotracheitis virus vaccinated steers challenged with infectious bovine rhinotracheitis virus Reprint Icon - (Peer Reviewed Journal)
Falkenberg, S.M., Carroll, J.A., Elsasser, T.H., Best, T., Sartin, J.L., Buntyn, J.O., Schmidt, T.B. 2013. Evaluation of endocrine and immune disruption of infectious bovine rhinotracheitis virus vaccinated steers challenged with infectious bovine rhinotracheitis virus. American Journal of Veterinary Research. 74(12):1552-1529.
In vitro neutralization against HoBi-like viruses by antiobodies in serum of cattle immunized with inactivated or modified live vaccines of bovine viral diarrhea virus 1 and 2 Reprint Icon - (Peer Reviewed Journal)
Bauermann, F.V., Harmon, A., Flores, E.F., Falkenberg, S.M., Reecy, J.M., Ridpath, J.F. 2013. In vitro neutralization against HoBi-like viruses by antiobodies in serum of cattle immunized with inactivated or modified live vaccines of bovine viral diarrhea virus 1 and 2. Veterinary Microbiology. 166(1-2):242-245 DOI: 10.1016/j.vetmic.2013.04.032.
Circulating immune cell subpopulations in pestivirus persistently infected calves and non-infected calves varying in immune status. - (Abstract Only)
Circulating immune cell subpopulations in pestivirus persistently infected calves and non-infected calves varying in immune status [Abstract] - (Proceedings)
Falkenberg, S.M., Ridpath, J.F., Bauermann, F.V. 2013. Circulating immune cell subpopulations in pestivirus persistently infected calves and non-infected calves varying in immune status [Abstract]. Journal of Animal Science Supplement. American Society of Animal Science Meeting, July 8-12, 2013,Indianapolis, Indiana. 91:649.
Association of hematological variables and castration status at the time of arrival at a research facility with the risk of bovine respiratory disease in beef calves Reprint Icon - (Peer Reviewed Journal)
Richeson, J.T., Pinedo, P.J., Kegley, E.B., Powell, J.G., Gadberry, M.S., Beck, P.A., Falkenberg, S.M. 2013. Association of hematological variables and castration status at the time of arrival at a research facility with the risk of bovine respiratory disease in beef calves. Journal of the American Veterinary Medical Association. 243(7):1035-1041. DOI: 10.2460/javma.243.7.1035.
Use of bioluminescent Escherichia coli to determine retention during the life cycle of the housefly, Musca domestica (Diptera: Muscidae, L) Reprint Icon - (Peer Reviewed Journal)
Schuster, G.L., Donaldson, J.R., Buntyn, J.O., Duoss, H.A., Callaway, T.R., Carroll, J.A., Falkenberg, S.M., Schmidt, T.B. 2013. Use of bioluminescent Escherichia coli to determine retention during the life cycle of the housefly, Musca domestica (Diptera: Muscidae, L). Foodborne Pathogens and Disease. 10:442-447.
Comparison of acute infection of calves exposed to a high-virulence or low-virulence bovine viral diarrhea virus or a HoBi-like virus Reprint Icon - (Peer Reviewed Journal)
Ridpath, J.F., Falkenberg, S.M., Bauermann, F.V., VanderLey, B.L., Do, Y., Flores, E.F., Rodman, D.M., Neill, J.D. 2013. Comparison of acute infection of calves exposed to a high-virulence or low-virulence bovine viral diarrhea virus or a HoBi-like virus. American Journal of Veterinary Research. 74(3):438-442. DOI: 10.2460/ajvr.74.3.438.
Correlation between circulating white blood cell counts and level of protective immune response against bovine viral diarrhea virus elicited by a modified live vaccine - (Abstract Only)