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United States Department of Agriculture

Agricultural Research Service

Related Topics

Gary P Richards
Food Safety and Intervention Technologies Research
Microbiology

Phone: (302) 857-6419
Fax: (302) 857-6451
Room 29

JAMES W.W. BAKER CENTER
DSU, 1200 N DUPONT HIGHWAY
DOVER , DE 199012277

Projects
Development of Detection and Intervention Technologies for Bacterial and Viral Pathogens Affecting Shellfish
Appropriated (D)
  Accession Number: 430252

Publications (Clicking on the reprint icon Reprint Icon will take you to the publication reprint.)
Persistence and elimination of human norovirus in food and on food contact surfaces: a critical review -
Cook, N., Knight, A., Richards, G.P. 2016. Persistence and elimination of human norovirus in food and on food contact surfaces: a critical review. Journal of Food Protection. Volume 79; Issue 7; Pgs. 1273-1294.
Hatchery mortalities of larval oysters caused by Vibrio tubiashii and Vibrio coralliilyticus -
Richards, G.P. 2016. Hatchery mortalities of larval oysters caused by Vibrio tubiashii and Vibrio coralliilyticus. Meeting Abstract. Aquaculture 2016, Las Vegas, NV, February 22-26, 2016, Page 666.
Purification and host specificity of predatory halobacteriovorax isolated from seawater -
Richards, G.P., Fay, J.P., Uknalis, J., Olanya, O.M., Watson, M.A. 2016. Purification and host specificity of predatory halobacteriovorax isolated from seawater. Applied and Environmental Microbiology. Volume 82: Number 3: Pages 922-927.
A critical review on the survival and elimination of norovirus in food and on food contact surfaces -
Cook, N., Knight, A., Richards, G.P., Stein, J. 2015. A critical review on the survival and elimination of norovirus in food and on food contact surfaces. Review Article. https://www.food.gov.uk/science/research/foodborneillness/b14programme/fs101120.
Complete genome sequence of the larval shellfish pathogen Vibrio Tubiashii type strain ATCC 19109 -
Richards, G.P., Needleman, D.S., Watson, M.A., Bono, J.L. 2014. Complete genome sequence of the larval shellfish pathogen Vibrio Tubiashii type strain ATCC 19109. Genome Announcements. 2(6); e01252-14. DOI: 10.1128/genomeA01252-14.
Mortalities of eastern and pacific oyster larvae caused by the pathogens Vibrio coralliilyticus and Vibrio tubiashii -
Richards, G.P., Watson, M.A., Needleman, D.S., Church, K.M., Hase, C.C. 2014. Mortalities of eastern and pacific oyster larvae caused by the pathogens Vibrio coralliilyticus and Vibrio tubiashii. Applied and Environmental Microbiology. 81:292-297.
Bacteriophage remediation of bacterial pathogens in aquaculture: a review of the technology -
Richards, G.P. 2014. Bacteriophage remediation of bacterial pathogens in aquaculture: a review of the technology. Bacteriophage. doi:10.4161/21597081.2014.975540.
Complete genome sequence for the shellfish pathogen Vibrio coralliilyticus RE98 isolated from a shellfish hatchery -
Richards, G.P., Bono, J.L., Watson, M.A., Needleman, D.S. 2014. Complete genome sequence for the shellfish pathogen Vibrio coralliilyticus RE98 isolated from a shellfish hatchery. Genome Announcements. DOI:10.1128/genomeA.01253-14.
Development of a two-step, non-probed multiplex real-time PCR for surveilling Vibrio anguillarum in seawater -
Hickey, M.E., Richards, G.P., Lee, J. 2014. Development of a two-step, non-probed multiplex real-time PCR for surveilling Vibrio anguillarum in seawater. Journal of Fish Diseases. 38:551-559.
Potential for bio-control of food-borne pathogens with Bacteriovorax spp. and implications for food safety -
Olanya, O.M., Richards, G.P. 2014. Potential for bio-control of food-borne pathogens with Bacteriovorax spp. and implications for food safety. Meeting Abstract. Proceedings of the 3rd International Conference and Exhibition on Food Processing and Technology. Las Vegas, Nevada. 2014. Volume 5: Page 47.
Shellfish-associated enteric virus illness: virus localization, disease outbreaks and prevention -
Richards, G.P. 2016. Shellfish-associated enteric virus illness: virus localization, disease outbreaks and prevention. Book Chapter.In: Goyal SM and Cannon JL (editors). Viruses in Foods. 2nd Edition Springer Nature, New York, NY. Pages 185-207.
Enteric virus and vibrio contamination of shellfish: intervention strategies -
Richards, G.P. 2013. Enteric virus and vibrio contamination of shellfish: intervention strategies. Symposium Proceedings. In: Proceedings of the Korean Society of Food Science and Nutrition.Food Safety for the Food Industry, Gwangju, South Kore, November 13-15,2013.pp.57-58
Loss of sigma factor RpoN increases intestinal colonization of vibrio parahaemolyticus in an adult mouse model" -
Whitaker, W., Richards, G.P., Boyd, E. 2014. Loss of sigma factor RpoN increases intestinal colonization of vibrio parahaemolyticus in an adult mouse model". Infection and Immunity. 82:544-556.
Seasonal levels of the Vibrio predator Bacteriovorax in Atlantic, Pacific and Gulf Coast Seawater -
Richards, G.P., Watson, M.A., Boyd, F., Burkhardt Iii, W., Lau, R., Uknalis, J., Fay, J.P. 2013. Seasonal levels of the Vibrio predator Bacteriovorax in Atlantic, Pacific and Gulf Coast Seawater. International Journal of Microbiology. doi:10.1155/2013/375371.
Detection of enteric viruses in shellfish -
Richards, G.P., Cliver, D.O., Greening, G.E. 2013. Detection of enteric viruses in shellfish. Meeting Proceedings. G. Sauve, editor In Molluscan Shellfish Safety, Proceedings of the 8th International Conference on Molluscan Shellfish Safety, Charlottetown, Prince Edward Island, Canada, June 12-17, 2011.Springer, New York, pp. 177-183.
An introduction to food and waterborne viruses: diseases, transmission, outbreaks, detection and control -
Cook, N., Richards, G.P. 2013. An introduction to food and waterborne viruses: diseases, transmission, outbreaks, detection and control. Book Chapter. Food and Waterborne Viruses, Woodhead Publishing Co, Cambridge, United Kingdom, pp.3-18..
Natural modulators of Vibrios in seawater and shellfish -
Murine macrophage inflammatory cytokine production and immune activation in response to Vibrio parahaemolyticus infection -
Waters, S., Luther, S., Joerger, T., Richards, G.P., Boyd, E., Parent, M.A. 2013. Murine macrophage inflammatory cytokine production and immune activation in response to Vibrio parahaemolyticus infection. Microbiology and Immunology. 57(4):323-328.
Resilience of norovirus GII.4 to freezing and thawing:implications for virus infectivity -
Richards, G.P., Watson, M.A., Meade, G.K., Hovan, G.L., Kingsley, D.H. 2012. Resilience of norovirus GII.4 to freezing and thawing:implications for virus infectivity. Food and Environmental Virology. 4:192-197.
Predatory bacteria as natural modulators of Vibrio parahaemolyticus and Vibrio vulnificus in seawater and oysters -
Richards, G.P., Fay, J.P., Dickens, K.A., Parent, M.A., Soroka, D.S., Boyd, E. 2012. Predatory bacteria as natural modulators of Vibrio parahaemolyticus and Vibrio vulnificus in seawater and oysters. Applied and Environmental Microbiology. 78:7455-7466.
Vibrio parahaemolyticus ToxRS regulator is required for stress tolerance and colonization in a novel orogastric streptomycin-induced adult murine model -
Whitaker, B.W., Parent, M.A., Aoife, B., Richards, G.P., Boyd, F.E. 2012. Vibrio parahaemolyticus ToxRS regulator is required for stress tolerance and colonization in a novel orogastric streptomycin-induced adult murine model. Infection and Immunity. 80:1834-1845.
Light scattering sensor for real-time identification of Vibrio parahaemolyticus, V. vulnificus and V. cholera colonies on solid agar plates -
Huff, K., Aroonnual, A., Bae, E., Banada, P., Rajwa, B., Rajwa, B., Hirleman, E.D., Robinson, J.P., Richards, G.P., Bhunia, A. 2012. Light scattering sensor for real-time identification of Vibrio parahaemolyticus, V. vulnificus and V. cholera colonies on solid agar plates. Microbial Biotechnology. DOI: 10.1111/j.1751-7915.2012.00349.x.
Water quality parameters and total aerobic bacterial and vibrionaceae loads in eastern oysters (Crassostrea virginica) from oyster gardening sites -
Fay, J., Richards, G.P., Ozbay, G. 2012. Water quality parameters and total aerobic bacterial and vibirionaceae loads in eastern oysters (crassostrea virginica) from oyster gardening sites. Archives of Environmental Contamination and Toxicology. 64:628-637.
Critical review of norovirus surrogates in food safety research: rationale for considering volunteer studies -
Richards, G.P. 2012. Critical review of norovirus surrogates in food safety research: rationale for considering volunteer studies. Food and Environmental Virology. 4:6-13.
Hemocytes are sites of persistence for virus-contaminated oysters -
Provost, K., Ozbay, G., Anderson, R., Richards, G.P., Kingsley, D.H. 2011. Hemocytes are sites of persistence for virus-contaminated oysters. Applied and Environmental Microbiology. 77:8360-8369.
Foodborne viruses -
Richards, G.P., Cliver, D.O., Greening, G. 2013. Foodborne viruses.In: S. Doores, Y. Salfinger and M.L. Tortorello. Compendium of Methods for the Microbiological Examination of Foods, 5th Edition, American Public Health Association, Washington, DC. Book Chapter. Doi:10.2105/MBEF.0222.049.
In memoriam Dean Otis Cliver 1935-2011 -
Richards, G.P. 2011. In memoriam Dean Otis Cliver 1935-2011. Food and Environmental Virology. 3(3):99-108. DOI: 10.1007/s12560-011-9064-7.
Human norovirus inactivation in oysters by high hydrostatic pressure processing: A randomized double-blinded study -
Leon, J., Kingsley, D.H., Montes, J., Richards, G.P., Lyon, G., Abdulhafid, G., Seitz, S., Fernandez, M., Teunis, P.F., Flick, G.J., Moe, C.L. 2011. Human norovirus inactivation in oysters by high hydrostatic pressure processing: A randomized double-blinded study. Journal of Infectious Diseases. 77(15):5476-82.
Sensitive detection of multiple hepatitis A virus genotypes with a single polony-based assay -
Inaccuracies in predicting human norovirus inactivation using surrogate viruses -
Richards, G.P. 2011. Inaccuracies in predicting human norovirus inactivation using surrogate viruses. Journal of Shellfish Research, Volume 30, Issue 2, P. 548.
Pursuit of methods to propagate norovirus: decades of research -
Richards, G.P. 2010. Pursuit of methods to propagate norovirus: decades of research. Pursuit of human norovirus propagation methods: decades of research. Edited by P.Lassus. Proceedings of the 7th International Conf. on Molluscan Shellfish Safety, Nantes, France, 14-19, June, 2009, pages 100-107
High pressure processing inactivates human norovirus within oysters -
Kingsley, D.H., Leon, J.S., Richards, G.P., Flick, G., Lyon, G.M., Abdulhafid, G., Sobolik, J., Creadore, A., Seitz, S., Smith, E., Fernandez, M., Moe, C. 2010. High pressure processing inactivates human norovirus within oysters [abstract]. Annual Meeting at American Society for Virology. p. 1.
Growth of Vibrio parahaemolyticus O3:K6 at Different Salt Concentrations Modulates Responses to pH and Temperature Stresses -
Whitaker, B.W., Michelle, P.A., Naughton, L.M., Richards, G.P., Blumerman, S.L., Boyd, F.E. 2010. Growth of Vibrio parahaemolyticus O3:K6 at Different Salt Concentrations Modulates Responses to pH and Temperature Stresses. Applied and Environmental Microbiology. 76:720-729.
Processing Strategies to Inactivate Enteric Viruses in Shellfish: Limitations of Surrogate Viruses and Molecular Methods -
Richards, G.P., Macleod, C., Le Guyader, F.S. 2010. Processing Strategies to Inactivate Enteric Viruses in Shellfish: Limitations of Surrogate Viruses and Molecular Methods. Food and Environmental Virology. Available: doi:10.107/s12560-010-9045-2.
Fluorogenic membrane overlays to enumerate total coliforms, Escherichia coli, and total Vibrionaceae in shellfish and seawater -
Richards, G.P., Watson, M.A. 2010. Fluorogenic membrane overlays to enumerate total coliforms, Escherichia coli, and total Vibrionaceae in shellfish and seawater. Internationl Journal of Microbiology. Vol 2010, Article ID 910486, http://www.hindawi.com/journals/ijmb/2010/910486.html.
The Influence of Feral Horse Activity on Water and Shellfish (Gukensia demissa) Quality Along the Western Coast of Assateague Island National Seashore, Maryland -
Lambert, M.S., Ozbay, G., Richards, G.P. 2009. The Influence of Feral Horse Activity on Water and Shellfish (Gukensia demissa) Quality Along the Western Coast of Assateague Island National Seashore, Maryland. Estuaries and Coasts - Journal of the Estuarine Research Federation. 57:405-415.
AN OVERVIEW OF ENTERIC VIRUS EXTRACTION AND ASSAY METHODS -
Richards, G.P. 2009. AN OVERVIEW OF ENTERIC VIRUS EXTRACTION AND ASSAY METHODS. In:P.Busby (ed)., Proceedings of the 6th Int'l Conference on Molluscan Shellfish Safety, Blenheim, New Zealand. pp.111-118.
SHEWANELLA AND PHOTOBACTERIUM IN OYSTERS AND SEAWATER FROM THE DELAWARE BAY -
Richards, G.P., Watson, M.A., Crane, E.J., Bushek, D., Burt, I. 2008. Shewanella and photobacterium in oysters and seawater from the delaware bay. Applied and Environmental Microbiology. 74(11):3323-3327.
AN OVERVIEW OF VIRUS EXTRACTION AND ASSAY METHODS -
Richards, G.P. 2007. An overview of virus extraction and assay methods. Abstract. 6th International Conference on Molluscan Shellfish Safety, Blenheim, New Zealand. p.78.
A SIMPLE FLUOROGENIC METHOD TO ASSESS VIBRIO CHOLERAE AND AEROMONAS HYDROPHILA IN WELL WATER -
Richards, G.P., Watson, M.A. 2006. A simple fluorogenic method to assess vibrio cholerae and aeromonas hydrophila in well water. American Journal of Tropical Medicine and Hygiene. 65(3):516-521
DETECTION AND IDENTIFICATION OF FOODBORNE PATHOGENS TO GENUS AND SPECIES LEVELS USING A NONINVASIVE MODIFIED LIGHT SCATTEROMETER - BARDOT -
Huff, K.R., Banada, P.P., Bae, E., Bayraktar, B., Rajwa, B., Robinson, J.P., Hirleman, E.D., Richards, G.P., Bhunia, A.K. 2006. Detection and identification of foodborne pathogens to genus and species levels using a noninvasive modified light scatterometer - bardot. American Society for Microbiology. P-075.
SPECIFICITY OF A VIBRIO VULNIFICUS AMINOPEPTIDASE TOWARD KININS AND OTHER PEPTIDYL SUBSTRATES -
Richards, G.P., Nunez, A. 2006. Specificity of a vibrio vulnificus aminopeptidase toward kinins and other peptidyl substrates. Journal of Bacteriology. Mar. 2006. p. 2056-2062.
SHELLFISH-ASSOCIATED VIRAL DISEASE OUTBREAKS -
Richards, G.P. 2006. Shellfish-associated viral disease outbreaks. Book Chapter. In Viruses in Foods, (S.M. Goyal, Editor), Springer, New York. pp. 223-238.
THE COLONY OVERLAY PROCEDURE FOR PEPTIDASES TO DETECT AND ENUMERATE TOTAL VIBRIONACEAE IN MOLLUSCAN SHELLFISH AND THEIR GROWING WATERS -
Richards, G.P. 2005. The colony overlay procedure for peptidases to detect and enumerate total Vibrionaceae in molluscan shellfish and their growing waters. First Interstate Shellfish Safety Conference/International Conference on Molluscan Shellfish Safety Joint Workshop and Integration of Science into Policy: Special Emphasis on Vibrios and Viruses Abstract p.33.
A NOVEL, ENZYME-BASED ASSAY FOR THE RAPID AND SIMPLE DETECTION OF VIBRIOS IN SHELLFISH AND SEAWATER -
Richards, G.P., Bushek, D. 2005. A novel, enzyme-based assay for the rapid and simple detection of vibrios in shellfish and seawater. International Association for Food Protection. Abstract P4-27. p. 130.
DIVERSITY AND SEASONALITY OF THE VIBRIONACEAE IN OYSTERS (CRASSOSTREA VIRGINICA)AND SEAWATER IN DELAWARE BAY -
Richards, G.P., Burt, I.G., David, B. 2005. Diversity and seasonality of the vibrionaceae in oysters (crassostrea virginica)and seawater in delaware bay. (Abstract). National Shellfisheries Association. Pg. 46.
DEVELOPMENT OF A SIMPLE AND RAPID FLUOROGENIC PROCEDURE FOR THE IDENTIFICATION OF VIBRIONACEAE FAMILY MEMBERS -
Richards, G.P., Watson, M.A., Parveen, S.A. 2005. Development of a simple and rapid fluorogenic procedure for the identification of vibrionaceae family members. Applied and Environmental Microbiology. 71:3524-3527.
A SURVEY FOR PHOSPHOGLUCOSE ISOMERASE WITH LYSYL AMINOPEPTIDASE ACTIVITY IN VIBRIONACEAE AND NON-VIBRIO PATHOGENS -
Richards, G.P., Parveen, S.A. 2005. A survey for phosphoglucose isomerase with lysyl aminopeptidase activity in vibrionaceae and non-vibrio pathogens. Biochimica et Biophysica Acta. 1748:128-133.
MOLECULAR METHODS TO DETECT ENTERIC VIRUSES IN MOLLUSCAN SHELLFISH -
Richards, G.P. 2004. Molecular methods to detect enteric viruses in molluscan shellfish. Proceedings of Molecular Methods to detect enteric viruses in molluscan shellfish. p.43-49.
DETECTION OF VIBRIOS IN OYSTERS CRASSOSTRTEA VIRGINICA AND SEAWATER USING A NEW, RAPID, SIMPLE, AND QUANTITATIVE ENZYME-BASED ASSAY -
Richards, G.P., Watson, M.A., Burt, I.G., Bushek, D. 2005. Detection of vibrios in oysters crassostrtea virginica and seawater using a new, rapid, simple, and quantitative enzyme-based assay. (Abstract). Aquaculture America. Paper # 8128.
STRUCTURAL AND FUNCTIONAL ANALYSES OF PHOSPHOGLUCOSE ISOMERASE FROM VIBRIO VULNIFICUS AND ITS LYSYL AMINOPEPTIDASE ACTIVITY -
Richards, G.P. 2004. Structural and functional analyses of phosphoglucose isomerase from Vibrio vulnificus and its lysyl aminopeptidase activity. Biochimica et Biophysica Acta. 1702:89-102.
GENOGROUP I AND II NOROVIRUSES DETECTED IN STOOL BY REAL-TIME REVERSE TRANSCRIPTION-PCR USING HIGHLY DEGENERATE, "UNIVERSAL" PRIMERS -
Richards, G.P., Watson, M.A., Frankhauser, R.L., Monroe, S.S. 2004. Genogroup I and II noroviruses detected in stool by real-time reverse transcription-PCR using highly degenerate, "universal" primers. Applied and Environmental Microbiology. Vol.70. No.12.
CHARACTERIZATION OF A LYSYL AMINOPEPTIDASE ACTIVITY ASSOCIATED WITH PHOSPHOGLUCOSE ISOMERASE OF VIBRIO VULNIFICUS -
Richards, G.P. 2004. Characterization of a lysyl aminopeptidase activity associated with phosphoglucose isomerase of vibrio vulnificus. Biochimica et Biophysica Acta. V.1700, 2. 219-229.
MOLECULAR METHODS TO DETECT ENTERIC VIRUSES IN MOLLUSCAN SHELLFISH -
Richards, G.P. 2004. Molecular methods to detect enteric viruses in molluscan shellfish. Meeting Abstract.
FOOD- AND WATER-BORNE ENTERIC VIRUSES -
Richards, G.P. 2005. Food- and water-borne enteric viruses. Foodborne Pathogens Microbial and Mol. Biology. (P.M. Frtamico, A.K. Bhumia, J.L. Smith) Caister Academic Press. Book Chapter. pg. 121-143
INACTIVATION OF ENTERIC VIRUSES WITH INTERVENTION TECHNOLOGIES -
Richards, G.P. 2004. Inactivation of enteric viruses with intervention technologies. (Abstract). World Aquaculture Society. p. 499.
CHARACTERIZATION OF LYSYL AMINOPEPTIDASE ACTIVITY FROM PHOSPHOGLUCOSE ISOMERASE OF VIBRIO VULNIFICUS -
Richards, G.P., Parveen, S., Hammer, C., Garfield, M. 2004. Characterization of lysyl aminopeptidase activity from phosphoglucose isomerase of vibrio vulnificus. Journal of Bacteriology. 1700(2):219-229.
A SYBR GREEN REAL-TIME RT-PCR METHOD TO DETECT AND QUANTITATE NORWALK VIRUS IN STOOLS -
RICHARDS, G.P., WATSON, M.A., KINGSLEY, D.H. A SYBR GREEN REAL-TIME RT-PCR METHOD TO DETECT AND QUANTITATE NORWALK VIRUS IN STOOLS. JOURNAL OF VIROLOGICAL METHODS. 2004. vol. 116. pg. 67-70.
INACTIVATION OF ENTERIC VIRUSES WITH INTERVENTION TECHNOLOGIES -
Richards, G.P. 2003. Inactivation of enteric viruses with intervention technologies. (Abstract). International Association of Food Protection. p. 188.
UPTAKE, PERSISTENCE, AND LOCALIZATION OF VIRULENT AND AVIRULENT VIBRIO VULNIFICUS IN THE EASTERN OYSTER, CRASSOSTREA VIRGINICA -
PARVEEN, S., WOLFE, W.C., RICHARDS, G.P. UPTAKE, PERSISTENCE, AND LOCALIZATION OF VIRULENT AND AVIRULENT VIBRIO VULNIFICUS IN THE EASTERN OYSTER, CRASSOSTREA VIRGINICA. MEETING ABSTRACT. 2003.
THE EVOLUTION OF MOLLUSCAN SHELLFISH SAFETY -
RICHARDS, G.P. THE EVOLUTION OF MOLLUSCAN SHELLFISH SAFETY. MEETING ABSTRACT. 2003. p. 221-245
PERSISTENCE OF HEPATITIS A VIRUS WITHIN OYSTERS -
KINGSLEY, D.H., RICHARDS, G.P. PERSISTENCE OF HEPATITIS A VIRUS WITHIN OYSTERS. JOURNAL OF FOOD PROTECTION. 2003. Vol. 66 (2) p. 331-334
GLANDULAR KALLIKREINS IN THE TELEOST CYPRINUS CARPIO: TISSUE DISTRIBUTION,POSSIBLE INVOLVEMENT IN PROLACTIN PROCESSING AND EFFECT OF 17 B-ESTRADIOL IN VIVO -
DETECTION OF BOTH HEPATITIS A VIRUS AND NORWALK-LIKE VIRUS IN IMPORTED CLAMS ASSOCIATED WITH FOOD-BORNE ILLNESS -
INACTIVATION OF HEPATITIS A AND A CALICIVIRUS BY HIGH HYDROSTATIC PRESSURE -
THE EVOLUTION OF MOLLUSCAN SHELLFISH SAFETY -
RICHARDS, G.P. THE EVOLUTION OF MOLLUSCAN SHELLFISH SAFETY. MEETING ABSTRACT. 2002. p.78
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