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United States Department of Agriculture

Agricultural Research Service

Related Topics

Ann Blechl
Crop Improvement and Genetics Research
Genetics

Phone: (510) 559-5716
Fax: (510) 559-5818
Room 2104

USDA, ARS, PWA, WRRC-CIU
800 BUCHANAN STREET
ALBANY , CA 94710

Projects
Improvement of Wheat Quality through Molecular Genetics
Appropriated (D)
  Accession Number: 424870
Molecular Tools for Improved Crop Biotechnology
Appropriated (D)
  Accession Number: 425198
Novel Promoters to Control Gene Expression in Crop Plants
Specific Cooperative Agreement (S)
  Accession Number: 429296
Mature Budwood: a New Explant for Agrobacterium-mediated Citrus Transformation
Specific Cooperative Agreement (S)
  Accession Number: 430575

Publications (Clicking on the reprint icon Reprint Icon will take you to the publication reprint.)
Advancing Crop Transformation in the Era of Genome Editing -
Alpeter, F., Springer, N., Bartley, L., Blechl, A.E., Brutnell, T., Citovsky, V., Conrad, L., Gelvin, S., Jackson, D., Kausch, A., Lemaux, P., Medford, J., Orozco-Cardenas, M., Tricoli, D., Van Eck, J., Voytas, D., Walbot, V., Wang, K., Zhang, Z., Stewart, C. 2016. Advancing Crop Transformation in the Era of Genome Editing. The Plant Cell. doi: http://dx.doi.org/10.1105/tpc.16.00196.
RNA interference targeting rye secalins alters flour protein composition in a wheat variety carrying a 1Bl.1RS translocation -
Blechl, A.E., Beecher, B.S., Vensel, W.H., Tanaka, C.K., Altenbach, S.B. 2016. RNA interference targeting rye secalins alters flour protein composition in a wheat variety carrying a 1Bl.1RS translocation. Journal of Cereal Science. 68:172-180.
The distribution of cotransformed transgenes in particle bombardment-mediated transformed wheat -
Han, Y., Blechl, A.E., Wang, D. 2015. The distribution of cotransformed transgenes in particle bombardment-mediated transformed wheat. Transgenic Research. doi: 10.1007/s11248-015-9906-4.
A bacterial gene codA encoding cytosine deaminase is an effective conditional negative selectable marker in Glycine max -
Shao, M., Michno, J., Blechl, A.E., Thomson, J.G. 2015. A bacterial gene codA encoding cytosine deaminase is an effective conditional negative selectable marker in Glycine max. BioMed Central (BMC)Biotechnology. doi: 10.1007/s00299-015-1818-5.
Wheat stripe rust resistance protein WKS1 reduces the ability of the thylakoid-associated ascorbate peroxidase to detoxify reactive oxygen species -
Gou, J., Li, K., Wu, K., Wang, X., Lin, H., Cantu, D., Uauy, C., Dobom-Alonso, A., Midorikawa, T., Inoue, K., Sanchez, J., Fu, D., Blechl, A.E., Wallington, E., Fahima, T., Meeta, M., Epstein, L., Dubcovsky, J. 2015. Wheat stripe rust resistance protein WKS1 reduces the ability of the thylakoid-associated ascorbate peroxidase to detoxify reactive oxygen species. The Plant Cell. 27:1755-1770.
Recombinase technology for precise genome engineering -
Thomson, J.G., Blechl, A.E. 2015. Recombinase technology for precise genome engineering. In: Zhang, F., Puchta, H., Thomson, J.G., editors. Advances in new technology for targeted modification of plant genomes. New York, NY: Springer. p. 113-144.
Molecular and genetic aspects of controlling the soilborne necrotrophic pathogens Rhizoctonia and Pythium -
Okubara, P.A., Dickman, M.B., Blechl, A.E. 2014. Molecular and genetic aspects of controlling the soilborne necrotrophic pathogens Rhizoctonia and Pythium. Plant Science. 128:61-70.
The wheat HMW-glutenin 1Dy10 gene promoter controls endosperm expression in Brachypodium distachyon Reprint Icon -
Thilmony, R.L., Guttman, M.E., Lin, J.W., Blechl, A.E. 2014. The wheat HMW-glutenin 1Dy10 gene promoter controls endosperm expression in Brachypodium distachyon. GM Crops. 5:36-43.
Host factors governing resistance to Rhizoctonia solani -
Okubara, P.A., Kogel, K., Blechl, A.E., Hulbert, S.H. 2013. Host factors governing resistance to Rhizoctonia solani. International Rhizoctonia Symposium. Abstract #10, p.31.
Variant high-molecular-weight glutenin subunits arising from biolistic transformation of wheat -
Blechl, A.E., Vensel, W.H. 2013. Variant high-molecular-weight glutenin subunits arising from biolistic transformation of wheat. Journal of Cereal Science. 57:496-503.
Effects of transgene-encoded high-molecular weight glutenin proteins in wheat flour blends and sponge and dough baking -
Graybosch, R.A., Seabourn, B.W., Chen, Y.R., Blechl, A.E. 2013. Effects of transgene-encoded high-molecular weight glutenin proteins in wheat flour blends and sponge and dough baking. Cereal Chemistry. 90(02): 164-168.
Physical characteristics of genetically-altered wheat related to technological protein separation -
Robertson, G.H., Blechl, A.E., Hurkman II, W.J., Anderson, O.D., Cao, T., Tanaka, C.K., Gregorski, K.S., Orts, W.J. 2013. Physical characteristics of genetically-altered wheat related to technological protein separation. Cereal Chemistry. 90(1):1-12.
Bxb1 recombinase mediates site-specific deletions in transgenic wheat -
Blechl, A.E., Lin, J.W., Shao, M., Thilmony, R.L., Thomson, J.G. 2012. Bxb1 recombinase mediates site-specific deletions in transgenic wheat. Plant Molecular Biology Reporter. doi:10.1007/s11105-012-0454-2.
Quality and agronomic effects of three high-molecular-weight glutenin subunit transgenic events in winter wheat -
Graybosch, R.A., Seabourn, B.W., Chen, Y.R., Blechl, A.E. 2010. Quality and agronomic effects of three high-molecular-weight glutenin subunit transgenic events in winter wheat. Cereal Chemistry. 88(1):95-102.
Expression of a truncated form of yeast ribosomal protein L3 in transgenic wheat improves resistance to Fusarium head blight -
Di, R., Blechl, A.E., Dill-Macky, R., Tortora, A., Tumer, N.E. 2010. Expression of a truncated form of yeast ribosomal protein L3 in transgenic wheat improves resistance to Fusarium head blight. Plant Science. 178: 374-380.
Applying Genetic Engineering to Understand the Contributions of Various Seed Proteins to Wheat Flour Properties -
Blechl, A.E., Lin, J.W., Altenbach, S.B., Hurkman Ii, W.J., Sloane, S.M., Tanaka, C.K., Vensel, W.H. 2010. Applying Genetic Engineering to Understand the Contributions of Various Seed Proteins to Wheat Flour Properties. Xth International Gluten Workshop, September 7-9, 2009, Clermont-Ferrand, France.
EFFECTS OF OVER-EXPRESSION OF HIGH MOLECULAR WEIGHT GLUTENIN SUBUNIT 1Dy10 ON WHEAT TORTILLA PROPERTIES -
Pierucci, V.R., Tilley, M., Graybosch, R.A., Blechl, A.E., Bean, S., Tilley, K.A. 2009. Effects of Overexpression of High Molecular Weight Glutenen Subunit 1Dy10 on Wheat Tortilla Properties. Journal of Agricultural and Food Chemistry. 57:6318-6326.
Wheat: Science and Trade -
Blechl, A.E., Jones, H.D. 2009. "Transgenic Applications in Wheat Improvement" In: Carver, Brett F, editor. Wheat: Science and Trade. Wiley-blackwell, Edison, NJ. p. 397-435.
Intragenic Applications for Wheat Genetic Engineering -
Blechl, A.E. 2009. Intragenic Applications for Wheat Genetic Engineering (abstract). Crop Improvement and Utilization Intragenics Workshop. p. 6.
Positional Cloning of a QTL for slow rusting in wheat -
Dubcovsky, J., Fu, D., Uauy, C., Blechl, A.E., Epstein, L., Chen, X., Distelfeld, A., Sela, H., Fahima, T. 2009. Positional Cloning of a QTL for slow rusting in wheat. [online only]. Available: http://www.intl-pag.org/17/abstracts/W61-PAGXVII-426.html.
A kinase-START gene confers temperature-dependent resistance to wheat stripe rust -
Fu, D., Uauy, C., Distelfeld, A., Blechl, A.E., Epstein, L., Chen, X., Sela, H., Fahima, T., Dubcovsky, J. 2009. A kinase-START gene confers temperature-dependent resistance to wheat stripe rust. Science. 323:1357-1360.
Stably Expressed D Genome-derived HMW Glutenin Subunit Genes Transformed Into Different Durum Wheat Genotypes Change Dough Mixing Properties -
Gadaleta, A., Blechl, A.E., Nguyen, S.B., Cardone, M.F., Ventura, M., Quick, J.S., Blanco, A. 2008. Stably Expressed D Genome-derived HMW Glutenin Subunit Genes Transformed Into Different Durum Wheat Genotypes Change Dough Mixing Properties. Molecular Breeding. 22:267-279.
A Transgenic Durum Wheat Line that is Free of Marker Genes and Expresses 1dy10 -
Gadaleta, A., Giancaspro, A., Blechl, A.E., Blanco, A. 2008. A Transgenic Durum Wheat Line that is Free of Marker Genes and Expresses 1dy10. Journal of Cereal Science. 48:439-445.
THE EXPRESSION OF A BEAN PGIP IN TRANSGENIC WHEAT CONFERS INCREASED RESISTANCE TO THE FUNGAL PATHOGEN BIPOLARIS SOROKINIANA -
Janni, M., Sella, L., Favaron, F., Blechl, A.E., De Lorenzo, G., D'Ovidio, R. 2008. The expression of a bean pgip in transgenic wheat confers increased resistance to the fungal pathogen bipolaris sorokiniana. Molecular Plant-Microbe Interactions.21:171-177.
RNA Interference for Wheat Functional Gene Analysis -
Fu, D., Uauy, C., Blechl, A.E., Dubcovsky, J. 2007. RNA interference for wheat functional gene analysis. Transgenic Research. 16:689-701.
Expression Patterns in Transgenic Wheats with Elevated Levels of Dx5 and/or Dy10 Glutenin Subunits -
Blechl, A.E., Lin, J.W., Nguyen, S., Dupont, F.M., Vensel, W.H., Chan, R., Anderson, O.D., Bregitzer, P.P., Fiedler, D.J. 2007. Expression Patterns in Transgenic Wheats with Elevated Levels of Dx5 and/or Dy10 Glutenin Subunits. In: George L. Lookhart, Perry K.W. Ng, editors. Gluten Proteins 2006. Minneapolis, MN: AACCI. p. 107-111.
Expression of the N-terminal 99 Amino Acids of Yeast Ribosomal Protein L3 in Transgenic Wheat Confers Resistance to Fusarium Head Blight -
Di, R., Blechl, A.E., Dill-Macky, R., Tortora, A., Tumer, N.E. 2007. Expression of the N-terminal 99 Amino Acids of Yeast Ribosomal Protein L3 in Transgenic Wheat Confers Resistance to Fusarium Head Blight. The 2nd International Conference on Plant Molecular Biology, March 23-27, 2007, Sanya, China. Molecular Plant Breeding. 5:283.
The TRI101 Story: Engineering Wheat and Barley to Resist Fusarium Head Blight -
Alexander, N.J., Blechl, A.E., Okubara, P.A., Mc Cormick, S.P., Manoharan, M., Dahleen, L.S., Kendra, D.F. 2006. The TRI101 Story: Engineering Wheat and Barley to Resist Fusarium Head Blight [abstract]. World Mycotoxin Forum, the Fourth Conference. p. 123.
The wheat and barley vernalization gene VRN3 is an orthologue of FT -
Yan, L., Fu, D., Li, C., Blechl, A.E., Tranquilli, G., Bonafede, M., Sanchez, A.N., Valarik, M., Yasuda, S., Dubcovsky, J. 2006. The wheat and barley vernalization gene VRN3 is an orthologue of FT. Proceedings of the National Academy of Sciences. 103: 19581-19586
A NAC Gene Regulating Senescence Improves Grain Protein, Zinc, and Iron Content in Wheat -
Uauy, C., Distelfeld, A., Fahima, T., Blechl, A.E., Dubcovsky, J. 2006. A NAC gene regulating senescence improves grain protein, zinc, and iron content in wheat. Science. 314(5803):1298-1301.
Expression patterns in transgenic wheats with elevated levels of Dx5 and/or Dy10 glutenin subunits -
Blechl, A.E., Lin, J.W. 2007. Expression patterns in transgenic wheats with elevated levels of Dx5 and/or Dy10 glutenin subunits. International Gluten Workshop. Gluten Proteins 2006, pg. 107-111. tb
COMPLEMENTATION OF THE PINA (NULL) ALLELE WITH THE WILD TYPE PINA SEQUENCE RESTORES A SOFT PHENOTYPE IN TRANSGENIC WHEAT -
Martin, J.M., Meyer, F.D., Smidansky, E.D., Wanjugi, H., Blechl, A.E., Giroux, M.J. 2006. Complementation of the pina (null) allele with the wild type pina sequence restores a soft phenotype in transgenic wheat. Theoretical and Applied Genetics. 113(8):1563-1570.
TRANSGENIC WHEATS WITH ELEVATED LEVELS OF DX5 AND/OR DY10 HIGH-MOLECULAR-WEIGHT GLUTENIN SUBUNITS YIELD DOUGHS WITH INCREASED MIXING STRENGTH AND TOLERANCE -
Blechl, A.E., Lin, J.W., Nguyen, S., Chan, R., Anderson, O.D., Dupont, F.M. 2007. Transgenic wheats with elevated levels of dx5 and/or dy10 high-molecular-weight glutenin subunits yield doughs with increased mixing strength and tolerance. Journal of Cereal Science. 45:172-183
PGPRO 1, A NOVEL BINARY VECTOR FOR MONOCOT PROMOTER CHARACTERIZATION -
Thilmony, R.L., Guttman, M.E., Chiniquy, D., Blechl, A.E. 2006. Pgpro 1, a novel binary vector for monocot promoter characterization. Plant Molecular Biology Reporter. 24:1-13, March 2006
CHANGES IN HIGH MOLECULAR WEIGHT GLUTENIN SUBUNIT COMPOSITION CAN BE GENETICALLY ENGINEERED WITHOUT IMPACTING WHEAT AGRONOMIC PERFORMANCE -
Bregitzer, P.P., Fiedler, D.J., Blechl, A.E., Lin, J.W., Sebesta, P.G., Fernandez De Soto, J., Chicaiza, O., Dubcovsky, J. 2006. Changes in high molecular weight glutenin subunit composition can be genetically engineered without impacting wheat agronomic performance. Crop Science. 46:1553-1563 (2006)
ACCUMULATION OF TRANSGENE-ENCODED DEFENSE-ASSOCIATED ENZYMES IN TISSUES VULNERABLE TO INITIAL FUSARIUM INFECTION -
Blechl, A.E., Somleva, M.N. Accumulation of transgene-encoded defense-associated enzymes in tissues vulnerable to initial fusarium infection. National Fusarium Head Blight Forum Proceedings. pp. 102
PHOSPHOMANNOSE ISOMERASE, PMI, AS A SELECTABLE MARKER GENE FOR DURUM WHEAT TRANSFORMATION -
Gadaleta, A., Giancaspro, A., Blechl, A.E., Blanco, A. 2006. Phosphomannose isomerase, pmi, as a selectable marker gene for durum wheat transformation. Journal of Cereal Science. 43: 31-37
REGULATION OF VRN-1 VERNALIZATION GENES IN NORMAL AND TRANSGENIC POLYPLOID WHEAT -
Loukoinov, A., Yan, L., Blechl, A.E., Sanchez, A., Dubcovsky, J. 2005. Regulation of vrn-1 vernalization genes in normal and transgenic polyploid wheat. Journal of Plant Physiology. Vol. 138, P. 1-10.
NEW APPROACHES TO OVERCOME FUSARIUM HEAD BLIGHT -
Blechl, A.E., Somleva, M.N., Okubara, P.A., Dill-Mackey, R. 2005. New approaches to overcome fusarium head blight. International Wheat Quality Conference. p. 85-95.
ATOMIC FORCE MICROSCOPY OF A HYBRID HIGH-MOLECULAR-WEIGHT GLUTENIN SUBUNIT FROM A TRANSGENIC HEXAPLOID WHEAT -
McIntire, T.M., Lew, E.J.L., Adalsteins, A.E., Blechl, A., Anderson, O.D., Brant, D.A., Kasarda, D.D. 2005. Atomic force microscopy of a hybrid high-molecular-weight glutenin subunit from a transgenic hexaploid wheat. Biopolymers. 78(2):53-61.
CHARACTERIZATION OF ORGAN SPECIFIC PROMOTERS IN TRANSGENIC WHEAT -
Somleva, M.N., Blechl, A.E. 2004. Characterization of organ specific promoters in transgenic wheat. National Fusarium Head Blight Forum Proceedings. P. 263-267
CHARACTERIZATION OF GLUTENIN POLYMERS IN A TRANSGENIC BREAD WHEAT LINE OVER-EXPRESSING A LMW-GS. -
Masci, S., D'Ovidio, R., Scossa, F., Patacchini, C., Lafiandra, D., Anderson, O.D., Blechl, A.E. 2004. Characterization of glutenin polymers in a transgenic bread wheat line over-expressing a LNW-GS. Proceedings - 8th Gluten Workshop. 1:10-13
AGRONOMIC, BIOCHEMICAL AND QUALITY CHARACTERISTICS OF WHEATS CONTAINING HMW-GLUTENIN TRANSGENES -
Blechl, A.E., Bregitzer, P.P., O'Brien, K., Lin, J.W., Nguyen, S.B., Anderson, O.D. 2004. Agronomic, biochemical and quality characteristics of wheats containing hmw-glutenin transgenes. Proceedings - 8th Gluten Workshop. p. 6-9.
THE WHEAT VRN2 GENE IS A FLOWERING REPRESSOR DOWNREGULATED BY VERNALIZATION -
Yan, L., Loukoinov, A., Blechl, A.E., Tranquilli, G., Ramakrishna, W., Sanmiguel, P., Bennetzen, J.L., Echenique, V., Dubcovsky, J. 2004. The wheat vrn2 gene is a flowering repressor downregulated by vernalization. Science. 303: 1640-1644
PRODUCTION AND CHARACTERIZATION OF A TRANSGENIC BREAD WHEAT LINE OVER-EXPRESSING A LOW-MOLECULAR WEIGHT GLUTENIN SUBUNIT GENE. -
MASCI, S., D'OVIDIO, R., SCOSSA, F., PATACCHINI, C., LAFIANDRA, D., ANDERSON, O.D., BLECHL, A.E. PRODUCTION AND CHARACTERIZATION OF A TRANSGENIC BREAD WHEAT LINE OVER-EXPRESSING A LOW-MOLECULAR WEIGHT GLUTENIN SUBUNIT GENE. MOLECULAR BREEDING. 2003. v. 12. p. 209-222.
TRANSGENE EXPRESSION IN SPRING WHEAT (TRITICUM AESTIVUM L.) TRANSFORMED WITH CANDIDATE ANTI-FUSARIUM GENES -
Somleva, M., Okubara, P.A., Blechl, A.E. 2002. Transgene expression in spring wheat (triticum aestivum l.) transformed with candidate anti-fusarium genes. National Fusarium Head Blight Forum Proceedings. 42-45
ENGINEERING DEOXYNIVALENOL METABOLISM IN WHEAT THROUGH THE EXPRESSION OF A FUGAL TRICHOTHECENE ACETYLTRANSFERASE GENE -
EXPRESSION OF TWO DIFFERENT CANDIDATE ANTI-FUSARIUM PROTEIN GENES AFFORDS PARTIAL PROTECTION AGAINST THE SPREAD OF FUSARIUM GRAMINEARUM -
TRANSGENIC APPROACHES TO COMBAT FUSARIUM HEAD BLIGHT IN WHEAT AND BARLEY. -
Dahleen, L.S., Okubara, P.A., Blechl, A.E. 2001. Transgenic approaches to combat fusarium head blight in wheat and barley. Crop Science 40:628-637.
TRANSGENIC APPROACHES TO COMBAT FUSARIUM HEAD BLIGHT IN WHEAT AND BARLEY. -
Dahleen, L.S., Okubara, P.A., Blechl, A.E. 1999. Transgenic approaches to combat fusarium head blight in wheat and barley. Agronomy Abstracts, p. 83.
ARS News Articles
Last Modified: 5/2/2016
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