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United States Department of Agriculture

Agricultural Research Service

Related Topics

Ann Blechl
Crop Improvement and Genetics Research
Genetics

Phone: (510) 559-5716
Fax: (510) 559-5818
Room 2104

USDA, ARS, PWA, WRRC-CIU
800 BUCHANAN STREET
ALBANY , CA 94710

Projects
Improvement of Wheat Quality through Molecular Genetics
Appropriated (D)
  Accession Number: 424870
Molecular Tools for Improved Crop Biotechnology
Appropriated (D)
  Accession Number: 425198
Novel Promoters to Control Gene Expression in Crop Plants
Specific Cooperative Agreement (S)
  Accession Number: 429296

Publications (Clicking on the reprint icon Reprint Icon will take you to the publication reprint.)
PvPGIP2 accumulation in specific floral tissues, but not in the endosperm, limits Fusarium graminearum infection in wheat -
Tundo, S., Janni, M., Moscetti, I., Mandala, G., Savatin, D., Blechl, A.E., Favaron, F., D'Ovidio, R. 2016. PvPGIP2 accumulation in specific floral tissues, but not in the endosperm, limits Fusarium graminearum infection in wheat. Molecular Plant-Microbe Interactions. 29:815-821.
Advancing Crop Transformation in the Era of Genome Editing -
Alpeter, F., Springer, N., Bartley, L., Blechl, A.E., Brutnell, T., Citovsky, V., Conrad, L., Gelvin, S., Jackson, D., Kausch, A., Lemaux, P., Medford, J., Orozco-Cardenas, M., Tricoli, D., Van Eck, J., Voytas, D., Walbot, V., Wang, K., Zhang, Z., Stewart, C. 2016. Advancing Crop Transformation in the Era of Genome Editing. The Plant Cell. doi: http://dx.doi.org/10.1105/tpc.16.00196.
RNA interference targeting rye secalins alters flour protein composition in a wheat variety carrying a 1Bl.1RS translocation -
Blechl, A.E., Beecher, B.S., Vensel, W.H., Tanaka, C.K., Altenbach, S.B. 2016. RNA interference targeting rye secalins alters flour protein composition in a wheat variety carrying a 1Bl.1RS translocation. Journal of Cereal Science. 68:172-180.
The distribution of cotransformed transgenes in particle bombardment-mediated transformed wheat -
Han, Y., Blechl, A.E., Wang, D. 2015. The distribution of cotransformed transgenes in particle bombardment-mediated transformed wheat. Transgenic Research. doi: 10.1007/s11248-015-9906-4.
A bacterial gene codA encoding cytosine deaminase is an effective conditional negative selectable marker in Glycine max -
Shao, M., Michno, J., Blechl, A.E., Thomson, J.G. 2015. A bacterial gene codA encoding cytosine deaminase is an effective conditional negative selectable marker in Glycine max. BioMed Central (BMC)Biotechnology. doi: 10.1007/s00299-015-1818-5.
Wheat stripe rust resistance protein WKS1 reduces the ability of the thylakoid-associated ascorbate peroxidase to detoxify reactive oxygen species -
Gou, J., Li, K., Wu, K., Wang, X., Lin, H., Cantu, D., Uauy, C., Dobom-Alonso, A., Midorikawa, T., Inoue, K., Sanchez, J., Fu, D., Blechl, A.E., Wallington, E., Fahima, T., Meeta, M., Epstein, L., Dubcovsky, J. 2015. Wheat stripe rust resistance protein WKS1 reduces the ability of the thylakoid-associated ascorbate peroxidase to detoxify reactive oxygen species. The Plant Cell. 27:1755-1770.
Recombinase technology for precise genome engineering -
Thomson, J.G., Blechl, A.E. 2015. Recombinase technology for precise genome engineering. In: Zhang, F., Puchta, H., Thomson, J.G., editors. Advances in new technology for targeted modification of plant genomes. New York, NY: Springer. p. 113-144.
Molecular and genetic aspects of controlling the soilborne necrotrophic pathogens Rhizoctonia and Pythium -
Okubara, P.A., Dickman, M.B., Blechl, A.E. 2014. Molecular and genetic aspects of controlling the soilborne necrotrophic pathogens Rhizoctonia and Pythium. Plant Science. 128:61-70.
The wheat HMW-glutenin 1Dy10 gene promoter controls endosperm expression in Brachypodium distachyon Reprint Icon -
Thilmony, R.L., Guttman, M.E., Lin, J.W., Blechl, A.E. 2014. The wheat HMW-glutenin 1Dy10 gene promoter controls endosperm expression in Brachypodium distachyon. GM Crops. 5:36-43.
Host factors governing resistance to Rhizoctonia solani -
Okubara, P.A., Kogel, K., Blechl, A.E., Hulbert, S.H. 2013. Host factors governing resistance to Rhizoctonia solani. International Rhizoctonia Symposium. Abstract #10, p.31.
Variant high-molecular-weight glutenin subunits arising from biolistic transformation of wheat -
Blechl, A.E., Vensel, W.H. 2013. Variant high-molecular-weight glutenin subunits arising from biolistic transformation of wheat. Journal of Cereal Science. 57:496-503.
Effects of transgene-encoded high-molecular weight glutenin proteins in wheat flour blends and sponge and dough baking -
Graybosch, R.A., Seabourn, B.W., Chen, Y.R., Blechl, A.E. 2013. Effects of transgene-encoded high-molecular weight glutenin proteins in wheat flour blends and sponge and dough baking. Cereal Chemistry. 90(02): 164-168.
Physical characteristics of genetically-altered wheat related to technological protein separation -
Robertson, G.H., Blechl, A.E., Hurkman II, W.J., Anderson, O.D., Cao, T., Tanaka, C.K., Gregorski, K.S., Orts, W.J. 2013. Physical characteristics of genetically-altered wheat related to technological protein separation. Cereal Chemistry. 90(1):1-12.
Bxb1 recombinase mediates site-specific deletions in transgenic wheat -
Blechl, A.E., Lin, J.W., Shao, M., Thilmony, R.L., Thomson, J.G. 2012. Bxb1 recombinase mediates site-specific deletions in transgenic wheat. Plant Molecular Biology Reporter. doi:10.1007/s11105-012-0454-2.
Quality and agronomic effects of three high-molecular-weight glutenin subunit transgenic events in winter wheat -
Graybosch, R.A., Seabourn, B.W., Chen, Y.R., Blechl, A.E. 2010. Quality and agronomic effects of three high-molecular-weight glutenin subunit transgenic events in winter wheat. Cereal Chemistry. 88(1):95-102.
Expression of a truncated form of yeast ribosomal protein L3 in transgenic wheat improves resistance to Fusarium head blight -
Di, R., Blechl, A.E., Dill-Macky, R., Tortora, A., Tumer, N.E. 2010. Expression of a truncated form of yeast ribosomal protein L3 in transgenic wheat improves resistance to Fusarium head blight. Plant Science. 178: 374-380.
Applying Genetic Engineering to Understand the Contributions of Various Seed Proteins to Wheat Flour Properties -
Blechl, A.E., Lin, J.W., Altenbach, S.B., Hurkman Ii, W.J., Sloane, S.M., Tanaka, C.K., Vensel, W.H. 2010. Applying Genetic Engineering to Understand the Contributions of Various Seed Proteins to Wheat Flour Properties. Xth International Gluten Workshop, September 7-9, 2009, Clermont-Ferrand, France.
EFFECTS OF OVER-EXPRESSION OF HIGH MOLECULAR WEIGHT GLUTENIN SUBUNIT 1Dy10 ON WHEAT TORTILLA PROPERTIES -
Pierucci, V.R., Tilley, M., Graybosch, R.A., Blechl, A.E., Bean, S., Tilley, K.A. 2009. Effects of Overexpression of High Molecular Weight Glutenen Subunit 1Dy10 on Wheat Tortilla Properties. Journal of Agricultural and Food Chemistry. 57:6318-6326.
Wheat: Science and Trade -
Blechl, A.E., Jones, H.D. 2009. "Transgenic Applications in Wheat Improvement" In: Carver, Brett F, editor. Wheat: Science and Trade. Wiley-blackwell, Edison, NJ. p. 397-435.
Intragenic Applications for Wheat Genetic Engineering -
Blechl, A.E. 2009. Intragenic Applications for Wheat Genetic Engineering (abstract). Crop Improvement and Utilization Intragenics Workshop. p. 6.
Positional Cloning of a QTL for slow rusting in wheat -
Dubcovsky, J., Fu, D., Uauy, C., Blechl, A.E., Epstein, L., Chen, X., Distelfeld, A., Sela, H., Fahima, T. 2009. Positional Cloning of a QTL for slow rusting in wheat. [online only]. Available: http://www.intl-pag.org/17/abstracts/W61-PAGXVII-426.html.
A kinase-START gene confers temperature-dependent resistance to wheat stripe rust -
Fu, D., Uauy, C., Distelfeld, A., Blechl, A.E., Epstein, L., Chen, X., Sela, H., Fahima, T., Dubcovsky, J. 2009. A kinase-START gene confers temperature-dependent resistance to wheat stripe rust. Science. 323:1357-1360.
Stably Expressed D Genome-derived HMW Glutenin Subunit Genes Transformed Into Different Durum Wheat Genotypes Change Dough Mixing Properties -
Gadaleta, A., Blechl, A.E., Nguyen, S.B., Cardone, M.F., Ventura, M., Quick, J.S., Blanco, A. 2008. Stably Expressed D Genome-derived HMW Glutenin Subunit Genes Transformed Into Different Durum Wheat Genotypes Change Dough Mixing Properties. Molecular Breeding. 22:267-279.
A Transgenic Durum Wheat Line that is Free of Marker Genes and Expresses 1dy10 -
Gadaleta, A., Giancaspro, A., Blechl, A.E., Blanco, A. 2008. A Transgenic Durum Wheat Line that is Free of Marker Genes and Expresses 1dy10. Journal of Cereal Science. 48:439-445.
THE EXPRESSION OF A BEAN PGIP IN TRANSGENIC WHEAT CONFERS INCREASED RESISTANCE TO THE FUNGAL PATHOGEN BIPOLARIS SOROKINIANA -
Janni, M., Sella, L., Favaron, F., Blechl, A.E., De Lorenzo, G., D'Ovidio, R. 2008. The expression of a bean pgip in transgenic wheat confers increased resistance to the fungal pathogen bipolaris sorokiniana. Molecular Plant-Microbe Interactions.21:171-177.
RNA Interference for Wheat Functional Gene Analysis -
Fu, D., Uauy, C., Blechl, A.E., Dubcovsky, J. 2007. RNA interference for wheat functional gene analysis. Transgenic Research. 16:689-701.
Expression Patterns in Transgenic Wheats with Elevated Levels of Dx5 and/or Dy10 Glutenin Subunits -
Blechl, A.E., Lin, J.W., Nguyen, S., Dupont, F.M., Vensel, W.H., Chan, R., Anderson, O.D., Bregitzer, P.P., Fiedler, D.J. 2007. Expression Patterns in Transgenic Wheats with Elevated Levels of Dx5 and/or Dy10 Glutenin Subunits. In: George L. Lookhart, Perry K.W. Ng, editors. Gluten Proteins 2006. Minneapolis, MN: AACCI. p. 107-111.
Expression of the N-terminal 99 Amino Acids of Yeast Ribosomal Protein L3 in Transgenic Wheat Confers Resistance to Fusarium Head Blight -
Di, R., Blechl, A.E., Dill-Macky, R., Tortora, A., Tumer, N.E. 2007. Expression of the N-terminal 99 Amino Acids of Yeast Ribosomal Protein L3 in Transgenic Wheat Confers Resistance to Fusarium Head Blight. The 2nd International Conference on Plant Molecular Biology, March 23-27, 2007, Sanya, China. Molecular Plant Breeding. 5:283.
The TRI101 Story: Engineering Wheat and Barley to Resist Fusarium Head Blight -
Alexander, N.J., Blechl, A.E., Okubara, P.A., Mc Cormick, S.P., Manoharan, M., Dahleen, L.S., Kendra, D.F. 2006. The TRI101 Story: Engineering Wheat and Barley to Resist Fusarium Head Blight [abstract]. World Mycotoxin Forum, the Fourth Conference. p. 123.
The wheat and barley vernalization gene VRN3 is an orthologue of FT -
Yan, L., Fu, D., Li, C., Blechl, A.E., Tranquilli, G., Bonafede, M., Sanchez, A.N., Valarik, M., Yasuda, S., Dubcovsky, J. 2006. The wheat and barley vernalization gene VRN3 is an orthologue of FT. Proceedings of the National Academy of Sciences. 103: 19581-19586
A NAC Gene Regulating Senescence Improves Grain Protein, Zinc, and Iron Content in Wheat -
Uauy, C., Distelfeld, A., Fahima, T., Blechl, A.E., Dubcovsky, J. 2006. A NAC gene regulating senescence improves grain protein, zinc, and iron content in wheat. Science. 314(5803):1298-1301.
Expression patterns in transgenic wheats with elevated levels of Dx5 and/or Dy10 glutenin subunits -
Blechl, A.E., Lin, J.W. 2007. Expression patterns in transgenic wheats with elevated levels of Dx5 and/or Dy10 glutenin subunits. International Gluten Workshop. Gluten Proteins 2006, pg. 107-111. tb
COMPLEMENTATION OF THE PINA (NULL) ALLELE WITH THE WILD TYPE PINA SEQUENCE RESTORES A SOFT PHENOTYPE IN TRANSGENIC WHEAT -
Martin, J.M., Meyer, F.D., Smidansky, E.D., Wanjugi, H., Blechl, A.E., Giroux, M.J. 2006. Complementation of the pina (null) allele with the wild type pina sequence restores a soft phenotype in transgenic wheat. Theoretical and Applied Genetics. 113(8):1563-1570.
TRANSGENIC WHEATS WITH ELEVATED LEVELS OF DX5 AND/OR DY10 HIGH-MOLECULAR-WEIGHT GLUTENIN SUBUNITS YIELD DOUGHS WITH INCREASED MIXING STRENGTH AND TOLERANCE -
Blechl, A.E., Lin, J.W., Nguyen, S., Chan, R., Anderson, O.D., Dupont, F.M. 2007. Transgenic wheats with elevated levels of dx5 and/or dy10 high-molecular-weight glutenin subunits yield doughs with increased mixing strength and tolerance. Journal of Cereal Science. 45:172-183
PGPRO 1, A NOVEL BINARY VECTOR FOR MONOCOT PROMOTER CHARACTERIZATION -
Thilmony, R.L., Guttman, M.E., Chiniquy, D., Blechl, A.E. 2006. Pgpro 1, a novel binary vector for monocot promoter characterization. Plant Molecular Biology Reporter. 24:1-13, March 2006
CHANGES IN HIGH MOLECULAR WEIGHT GLUTENIN SUBUNIT COMPOSITION CAN BE GENETICALLY ENGINEERED WITHOUT IMPACTING WHEAT AGRONOMIC PERFORMANCE -
Bregitzer, P.P., Fiedler, D.J., Blechl, A.E., Lin, J.W., Sebesta, P.G., Fernandez De Soto, J., Chicaiza, O., Dubcovsky, J. 2006. Changes in high molecular weight glutenin subunit composition can be genetically engineered without impacting wheat agronomic performance. Crop Science. 46:1553-1563 (2006)
ACCUMULATION OF TRANSGENE-ENCODED DEFENSE-ASSOCIATED ENZYMES IN TISSUES VULNERABLE TO INITIAL FUSARIUM INFECTION -
Blechl, A.E., Somleva, M.N. Accumulation of transgene-encoded defense-associated enzymes in tissues vulnerable to initial fusarium infection. National Fusarium Head Blight Forum Proceedings. pp. 102
PHOSPHOMANNOSE ISOMERASE, PMI, AS A SELECTABLE MARKER GENE FOR DURUM WHEAT TRANSFORMATION -
Gadaleta, A., Giancaspro, A., Blechl, A.E., Blanco, A. 2006. Phosphomannose isomerase, pmi, as a selectable marker gene for durum wheat transformation. Journal of Cereal Science. 43: 31-37
REGULATION OF VRN-1 VERNALIZATION GENES IN NORMAL AND TRANSGENIC POLYPLOID WHEAT -
Loukoinov, A., Yan, L., Blechl, A.E., Sanchez, A., Dubcovsky, J. 2005. Regulation of vrn-1 vernalization genes in normal and transgenic polyploid wheat. Journal of Plant Physiology. Vol. 138, P. 1-10.
NEW APPROACHES TO OVERCOME FUSARIUM HEAD BLIGHT -
Blechl, A.E., Somleva, M.N., Okubara, P.A., Dill-Mackey, R. 2005. New approaches to overcome fusarium head blight. International Wheat Quality Conference. p. 85-95.
ATOMIC FORCE MICROSCOPY OF A HYBRID HIGH-MOLECULAR-WEIGHT GLUTENIN SUBUNIT FROM A TRANSGENIC HEXAPLOID WHEAT -
McIntire, T.M., Lew, E.J.L., Adalsteins, A.E., Blechl, A., Anderson, O.D., Brant, D.A., Kasarda, D.D. 2005. Atomic force microscopy of a hybrid high-molecular-weight glutenin subunit from a transgenic hexaploid wheat. Biopolymers. 78(2):53-61.
CHARACTERIZATION OF ORGAN SPECIFIC PROMOTERS IN TRANSGENIC WHEAT -
Somleva, M.N., Blechl, A.E. 2004. Characterization of organ specific promoters in transgenic wheat. National Fusarium Head Blight Forum Proceedings. P. 263-267
AGRONOMIC, BIOCHEMICAL AND QUALITY CHARACTERISTICS OF WHEATS CONTAINING HMW-GLUTENIN TRANSGENES -
Blechl, A.E., Bregitzer, P.P., O'Brien, K., Lin, J.W., Nguyen, S.B., Anderson, O.D. 2004. Agronomic, biochemical and quality characteristics of wheats containing hmw-glutenin transgenes. Proceedings - 8th Gluten Workshop. p. 6-9.
CHARACTERIZATION OF GLUTENIN POLYMERS IN A TRANSGENIC BREAD WHEAT LINE OVER-EXPRESSING A LMW-GS. -
Masci, S., D'Ovidio, R., Scossa, F., Patacchini, C., Lafiandra, D., Anderson, O.D., Blechl, A.E. 2004. Characterization of glutenin polymers in a transgenic bread wheat line over-expressing a LNW-GS. Proceedings - 8th Gluten Workshop. 1:10-13
THE WHEAT VRN2 GENE IS A FLOWERING REPRESSOR DOWNREGULATED BY VERNALIZATION -
Yan, L., Loukoinov, A., Blechl, A.E., Tranquilli, G., Ramakrishna, W., Sanmiguel, P., Bennetzen, J.L., Echenique, V., Dubcovsky, J. 2004. The wheat vrn2 gene is a flowering repressor downregulated by vernalization. Science. 303: 1640-1644
PRODUCTION AND CHARACTERIZATION OF A TRANSGENIC BREAD WHEAT LINE OVER-EXPRESSING A LOW-MOLECULAR WEIGHT GLUTENIN SUBUNIT GENE. -
MASCI, S., D'OVIDIO, R., SCOSSA, F., PATACCHINI, C., LAFIANDRA, D., ANDERSON, O.D., BLECHL, A.E. PRODUCTION AND CHARACTERIZATION OF A TRANSGENIC BREAD WHEAT LINE OVER-EXPRESSING A LOW-MOLECULAR WEIGHT GLUTENIN SUBUNIT GENE. MOLECULAR BREEDING. 2003. v. 12. p. 209-222.
TRANSGENE EXPRESSION IN SPRING WHEAT (TRITICUM AESTIVUM L.) TRANSFORMED WITH CANDIDATE ANTI-FUSARIUM GENES -
Somleva, M., Okubara, P.A., Blechl, A.E. 2002. Transgene expression in spring wheat (triticum aestivum l.) transformed with candidate anti-fusarium genes. National Fusarium Head Blight Forum Proceedings. 42-45
ENGINEERING DEOXYNIVALENOL METABOLISM IN WHEAT THROUGH THE EXPRESSION OF A FUGAL TRICHOTHECENE ACETYLTRANSFERASE GENE -
EXPRESSION OF TWO DIFFERENT CANDIDATE ANTI-FUSARIUM PROTEIN GENES AFFORDS PARTIAL PROTECTION AGAINST THE SPREAD OF FUSARIUM GRAMINEARUM -
TRANSGENIC APPROACHES TO COMBAT FUSARIUM HEAD BLIGHT IN WHEAT AND BARLEY. -
Dahleen, L.S., Okubara, P.A., Blechl, A.E. 2001. Transgenic approaches to combat fusarium head blight in wheat and barley. Crop Science 40:628-637.
TRANSGENIC APPROACHES TO COMBAT FUSARIUM HEAD BLIGHT IN WHEAT AND BARLEY. -
Dahleen, L.S., Okubara, P.A., Blechl, A.E. 1999. Transgenic approaches to combat fusarium head blight in wheat and barley. Agronomy Abstracts, p. 83.
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Last Modified: 5/2/2016
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