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United States Department of Agriculture

Agricultural Research Service

Related Topics

Stephanie Patfield
Foodborne Toxin Detection and Prevention Research
Biological Science Lab Technician

Phone: (510) 559-5657
Fax: (510) 559-6429

USDA, ARS, PWA, WRRC-FTDP
800 BUCHANAN ST
ALBANY , CA 94710

Publications (Clicking on the reprint icon Reprint Icon will take you to the publication reprint.)
Overcoming the challenges of safely quantifying and distinguishing among Shiga toxins in complex media and human serum -
Silva, C.J., Erickson-Beltran, M.L., Skinner, C.B., Patfield, S.A., He, X., Wu, V.C. 2017. Overcoming the challenges of safely quantifying and distinguishing among Shiga toxins in complex media and human serum. Meeting Abstract. Poster. ARS-FSIS 2017 meeting.
Detecting and distinguishing among type 1 and type 2 Shiga toxins in human serum -
Silva, C.J., Erickson-Beltran, M.L., Skinner, C.B., Patfield, S.A., He, X. 2017. Detecting and distinguishing among type 1 and type 2 Shiga toxins in human serum. Abstracts of the Papers of the American Chemical Society. 253:234-AGFD.
Validation of two new immunoassays for sensative detection of a broad range of shiga toxins Reprint Icon -
Kong, Q., Patfield, S.A., Skinner, C.B., Stanker, L.H., Gehring, A.G., Fratamico, P.M., Rubio, F., Qi, W., He, X. 2016. Validation of two new immunoassays for sensative detection of a broad range of shiga toxins. Austin Immunology. 1(2):1007.
Detecting and discriminating among Shiga toxins -
Silva, C.J., Erickson-Beltran, M.L., Skinner, C.B., Patfield, S.A., He, X., Wu, V.C. 2016. Detecting and discriminating among Shiga toxins. doi: 10.1021/acs.jafc.6b00379.
Phage-mediated Shiga toxin (Stx) horizontal gene transfer and expression in non-Shiga toxigenic Enterobacter and Escherichia coli strains -
Khalil, R., Skinner, C.B., Patfield, S.A., He, X. 2016. Phage-mediated Shiga toxin (Stx) horizontal gene transfer and expression in non-Shiga toxigenic Enterobacter and Escherichia coli strains. Pathogens and Disease. 74(5):1-11. doi: 10.1093/femspd/ftw037.
Safe, rapid, and sensitive method of quantitating and distinguishing among Shiga toxins in complex media and human serum -
Wu, V.C., Silva, C.J., Erickson-Beltran, M.L., Skinner, C.B., Patfield, S.A., He, X. 2016. Safe, rapid, and sensitive method of quantitating and distinguishing among Shiga toxins in complex media and human serum. ARS Food Safety and Inspection Service Research Workshop. ARS-FSIS.
New monoclonal antibodies against a novel subtype of Shiga toxin 1 produced by Enterobacter cloacae and their use in analysis of human serum Reprint Icon -
Skinner, C.B., Patfield, S.A., Khalil, R., Kong, Q., He, X. 2016. New monoclonal antibodies against a novel subtype of Shiga toxin 1 produced by Enterobacter cloacae and their use in analysis of human serum. mSphere. 1(1):e00099-15.
A new immunoassay for detecting all subtypes of Shiga toxins produced by Shiga toxin-producing E. coli in ground beef Reprint Icon -
He, X., Kong, Q., Patfield, S.A., Skinner, C.B., Rasooly, R. 2016. A new immunoassay for detecting all subtypes of Shiga toxins produced by Shiga toxin-producing E. coli in ground beef. PLoS One. 11(1):e0148092.
Analysis of castor by ELISAs that distinguish Ricin and Ricinus communis agglutinin (RCA) -
Brandon, D.L., McKeon, T.A., Patfield, S.A., He, X. 2016. Analysis of castor by ELISAs that distinguish Ricin and Ricinus communis agglutinin (RCA). Journal of the American Oil Chemists' Society. 93:359-363.
Evaluation of castor oil samples for potential toxin contamination -
Mckeon, T.A., Patfield, S.A., He, X. 2016. Evaluation of castor oil samples for potential toxin contamination. Industrial Crops and Products. 93:299-301.
Mass spectrometry-based method of detecting and distinguishing type 1 and type 2 Shiga-like toxins in human serum Reprint Icon -
Silva, C.J., Erickson-Beltran, M.L., Skinner, C.B., Patfield, S.A., He, X. 2015. Mass spectrometry-based method of detecting and distinguishing type 1 and type 2 Shiga-like toxins in human serum. Toxins. 7:5236-5253.
Immuno-PCR assay for sensitive detection of proteins in real time -
He, X., Patfield, S.A. 2016. Immuno-PCR assay for sensitive detection of proteins in real time. Methods in Molecular Biology. 1318:139-148 doi: 10.1007/978-1-4939-2742-5_14.
An in vitro combined antibiotic/antibody treatment eliminates toxicity from Shiga toxin-producing E. coli Reprint Icon -
Skinner, C.B., Patfield, S.A., He, X., Zhang, G. 2015. An in vitro combined antibiotic/antibody treatment eliminates toxicity from Shiga toxin-producing E. coli. Antimicrobial Agents and Chemotherapy. 59(9):5435-5444.
New Stx2e monoclonal antibodies for immunological detection and distinction of Stx2 subtypes Reprint Icon -
Skinner, C.B., Patfield, S.A., Hernlem, B.J., He, X. 2015. New Stx2e monoclonal antibodies for immunological detection and distinction of Stx2 subtypes. PLoS One. 10(7):e0132419.
New high-affinity monoclonal antibodies against Shiga toxin 1 facilitate the detection of hybrid Stx1/Stx2 in vivo -
Skinner, C.B., Patfield, S.A., Stanker, L.H., Fratamico, P.M., He, X. 2014. New high-affinity monoclonal antibodies against Shiga toxin 1 facilitate the detection of hybrid Stx1/Stx2 in vivo. PLoS One. 9(6):e99854. DOI:10.1371journal.pone0099854.
Safe and effective means of detecting and quantitating Shiga-like toxins in attomole amounts Reprint Icon -
Silva, C.J., Erickson-Beltran, M.L., Skinner, C.B., Dynin, I., Hui, C., Patfield, S.A., Carter, J.M., He, X. 2014. Safe and effective means of detecting and quantitating Shiga-like toxins in attomole amounts. Analytical Chemistry. 86(10):4698-4706.
Using mass spectrometry to detect and discriminate among Shiga-like toxins in the attomole range. Reprint Icon -
Silva, C.J., Erickson-Beltran, M.L., Skinner, C.B., Dynin, I.A., Hui, C., Patfield, S.A., He, X. 2014. Using mass spectrometry to detect and discriminate among Shiga-like toxins in the attomole range [abstract]. Meeting Abstract. Innovations in Mass Spectrometry. Paper No. 355.
Mouse in vivo neutralization of Escherichia coli Shiga toxin 2 with monoclonal antibodies Reprint Icon -
Cheng, L.W., Henderson II, T.D., Patfield, S.A., Stanker, L.H., He, X. 2013. Mouse in vivo neutralization of Escherichia coli Shiga toxin 2 with monoclonal antibodies. Infection and Immunity. 5:1845-1858. DOI: 10.3390/toxins5101845.
Development of monoclonal antibodies and immunoassays for sensitive and specific detection of Shiga Toxin Stx2f -
Skinner, C.B., Patfield, S.A., Stanker, L.H., He, X. 2013. Development of monoclonal antibodies and immunoassays for sensitive and specific detection of Shiga Toxin Stx2f. PLoS One. 8(9):e76563 DOI: 10.1371/journal/pone.0076563.
A polyclonal antibody based immunoassay detects seven subtypes of Shiga toxin 2 produced by escherichia coli in human and environmental samples -
He, X., Patfield, S.A., Hnasko, R.M., Rasooly, R., Mandrell, R.E. 2013. A polyclonal antibody based immunoassay detects seven subtypes of Shiga toxin 2 produced by escherichia coli in human and environmental samples. PLoS One. 8(10):e76368.
Purification and characterization of Shiga toxin 2f, an immunologically unrelated subtype of Shiga toxin 2 Reprint Icon -
Skinner, C.B., Patfield, S.A., Rasooly, R., Carter, J.M., He, X. 2013. Purification and characterization of Shiga toxin 2f, an immunologically unrelated subtype of Shiga toxin 2. PLoS One. 8(3):e59760.
Development and characterization of monoclonal antibodies against Shiga toxin 2 and their application for toxin detection in milk -
He, X., Mcmahon, S.A., Skinner, C.B., Merrill, P.A., Scotcher, M., Stanker, L.H. 2013. Development and characterization of monoclonal antibodies against Shiga toxin 2 and their application for toxin detection in milk. Journal of Immunological Methods. 389(1-2):18-28. doi:10.1016/j.jim.2012.12.005.
A single-step purification and molecular characterization of functional Shiga toxin 2 variants from pathogenic Escherichia coli -
He, X., Quinones, B., Mcmahon, S.A., Mandrell, R.E. 2012. A single-step purification and molecular characterization of functional Shiga toxin 2 variants from pathogenic Escherichia coli. Toxins. 4(7):487-504. doi:10.3390/toxins4070487
Evaluation and comparison of three enzyme-linked immunosorbent assay formats for the detection of ricin in milk and serum -
He, X., Mcmahon, S.A., Rasooly, R. 2012. Evaluation and comparison of three enzyme-linked immunosorbent assay formats for the detection of ricin in milk and serum. Biocatalysis and Agricultural Biotechnology. 1(2):105-109. doi:10.1016/j.bcab.2011.08.016.
Comparison of a high-density genetic linkage map to genome features in the model grass Brachypodium distachyon -
Huo, N., Garvin, D.F., You, F., McMahon, S.A., Luo, M., Gu, Y.Q., Lazo, G.R., Vogel, J.P. 2011. Comparison of a high-density genetic linkage map to genome features in the model grass Brachypodium distachyon. Theoretical and Applied Genetics. 123(3):455-464.
Sensitive detection of Shiga toxin 2 and some of its Stx 2 variants in environmental samples by a novel immuno-PCR assay -
He, X., Qi, W., Quinones, B., Mcmahon, S.A., Cooley, M.B., Mandrell, R.E. 2011. Sensitive detection of Shiga toxin 2 and some of its Stx 2 variants in environmental samples by a novel immuno-PCR assay. Applied and Environmental Microbiology. 77(1):3558-3564. doi:10.1128/AEM.02205-10.
Ricin toxicokinetics and its sensitive detection in mouse sera or feces using immuno-PCR -
He, X., Mcmahon, S.A., Henderson II, T.D., Griffey, S.M., Cheng, L.W. 2010. Ricin toxicokinetics and its sensitive detection in mouse sera or feces using immuno-PCR. PLoS One. 5(9):e12858. doi:10.1371/journal.pone.0012858.
Development of a Novel Immuno-PCR Assay for Detection of Ricin in Ground Beef, Liquid Chicken Egg and Milk -
He, X., McMahon, S.A., McKeon, T.A., Brandon, D.L. 2010. Development of a Novel Immuno-PCR Assay for Detection of Ricin in Ground Beef, Liquid Chicken Egg and Milk. Journal of Food Protection. 73(4):695-700
Structural characterization of Brachypodium genome and its syntenic relationship with rice and wheat -
Huo, N., Vogel, J.P., Lazo, G.R., You, F., Ma, Y., McMahon, S.A., Dvorak, J., Anderson, O.D., Luo, M., Gu, Y.Q. 2009. Structural characterization of Brachypodium genome and its syntenic relationship with rice and wheat. Plant Molecular Biology. 70: 47-61.
Last Modified: 05/24/2017
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