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United States Department of Agriculture

Agricultural Research Service

Related Topics

Kurt Wagschal
Bioproducts Research
Chemistry

Phone: (510) 559-6453
Fax: (510) 559-5818
Room 3109

USDA, ARS, PWA, WRRC-BCE
800 BUCHANAN STREET
ALBANY , CA 94710

Projects
Technologies for Improving Industrial Biorefineries that Produce Marketable Biobased Products
Appropriated (D)
  Accession Number: 427427

Publications (Clicking on the reprint icon Reprint Icon will take you to the publication reprint.)
Expression and characterization of hyperthermostable exo-polygalacturonase TtGH28 from Thermotoga thermophilus -
Wagschal, K.C., Stoller, J.R., Chan, V.J., Lee, C.C., Grigorescu, A.A., Jordan, D.B. 2016. Expression and characterization of hyperthermostable exo-polygalacturonase TtGH28 from Thermotoga thermophilus. Molecular Biotechnology. 58(7):509-519. doi 10.1007/s12033-016-9948-8.
Biochemical characterization of a GH43 ß-xylosidase from Bacteroides ovatus -
X-ray crystal structure of divalent metal-activated ß-xyloisdase, RS223BX Reprint Icon -
Jordan, D.B., Braker, J.D., Wagschal, K., Lee, C.C., Chan, V.J., Dubrovska, I., Anderson, S., Wawrzak, Z. 2015. X-ray crystal structure of divalent metal-activated ß-xyloisdase, RS223BX. Applied Biochemistry and Biotechnology. 177:637-648. doi: 10.1007/s12010-015-1767-z.
Nanostructure enzyme assemblies for biomass conversion -
Biochemical characterization of uronate dehydrogenases from three Pseudomonads, Chromohalobacter salixigens, and Polaromonas naphthalenivorans Reprint Icon -
Wagschal, K.C., Jordan, D.B., Lee, C.C., Younger, A.R., Braker, J.D., Chan, V.J. 2014. Biochemical characterization of uronate dehydrogenases from three Pseudomonads, Chromohalobacter salixigens, and Polaromonas naphthalenivorans. Enzyme and Microbial Technology. 69:62-68.
Directed evolution of GH43 ß-xylosidase XylBH43 thermal stability and L186 saturation Reprint Icon -
Singh, S.K., Heng, C., Braker, J.D., Chan, V.J., Lee, C.C., Jordan, D.B., Yuan, L., Wagschal, K.C. 2013. Directed evolution of GH43 ß-xylosidase XylBH43 thermal stability and L186 saturation. Journal of Industrial Microbiology and Biotechnology. 41(3):489-498. doi: 10.1007/s10295-013-1377-0.
Activation of a GH43 ß-xylosidase by divalent metal cations: Slow binding of divalent metal and high substrate specificity -
Jordan, D.B., Lee, C.C., Wagschal, K., Braker, J.D. 2013. Activation of a GH43 ß-xylosidase by divalent metal cations: Slow binding of divalent metal and high substrate specificity. Archives of Biochemistry and Biophysics. 533:79-87.
Divalent metal activation of a GH43 ß-xylosidase -
Lee, C.C., Braker, J.D., Grigorescu, A.A., Wagschal, K.C., Jordan, D.B. 2013. Divalent metal activation of a GH43 ß-xylosidase. Enzyme and Microbial Technology. 52(2):84-90.
Highly active ß-xylosidases of glycoside hydrolase family 43 operating on natural and artificial substrates -
Jordan, D.B., Wagschal, K.C., Grigorescu, A.A., Braker, J.D. 2013. Highly active ß-xylosidases of glycoside hydrolase family 43 operating on natural and artificial substrates. Applied Microbiology and Biotechnology. 97:4415-4428.
Designer xylanosomes: protein nanostructures for enhanced xylan hydrolysis -
Mcclendon, S.D., Mao, Z., Shin, H., Wagschal, K.C., Chen, R.R. 2012. Designer xylanosomes: protein nanostructures for enhanced xylan hydrolysis. Biomacromolecules. 167:385-411.
Isolation and characterization of a novel GH67 a-glucuronidase from a mixed culture -
Lee, C.C., Wagschal, K.C., Ruiping, L., Robertson, G.H., Kibblewhite, R.E., Orts, W.J. 2012. Isolation and characterization of a novel GH67 a-glucuronidase from a mixed culture. Journal of Industrial Microbiology and Biotechnology. 39:1245-1251. DOI:10.1007/s10295-012-1128-7.
Development of enzymes and enzyme systems by genetic engineering to convert biomass to sugars -
Lee, C.C., Wagschal, K.C. 2013. Development of enzymes and enzyme systems by genetic engineering to convert biomass to sugars. [Abstract}. California American Chemical Society Section Meeting, April 25, 2012, Albany CA.
Nanostructured enzyme assemblies for lignocellulosic biomass breakdown for bioproduct and bioenergy applications -
Microplate-based active/inactive 1 screen for biomass degrading enzyme library purification and gene discovery -
Wagschal, K.C., Lee, C.C. 2012. Microplate-based active/inactive 1 screen for biomass degrading enzyme library purification and gene discovery. Analytical Biochemistry. 89: 83-85.
Plant cell walls to ethanol. -
Jordan, D.B., Bowman, M.J., Braker, J.D., Dien, B.S., Hector, R.E., Lee, C.C., Mertens, J.A., Wagschal, K.C. 2012. Plant cell walls to ethanol. Biochemical Journal. 442:247-252.
Isolation of alpha-glucuronidase enzyme from a rumen metagenomic library -
Lee, C.C., Kibblewhite, R.E., Wagschal, K.C., Li, R., Orts, W.J. 2012. Isolation of alpha-glucuronidase enzyme from a rumen metagenomic library. Protein Journal. 31(3):206-211.
Catalytic properties of ß-D-xylosidase XylBH43 from Bacillus halodurans C-125 and mutant XylBH43-W147G -
Wagschal, K.C., Jordan, D.B., Braker, J.D. 2011. Expression, characterization, and site-directed mutagenesis of ß-D-xylosidase XylBH43 from Bacillus halodurans C-125. Process Biochemistry. doi:10.1016/j.procbio.2011.07.009.
Engineering lower inhibitor affinities in beta-D-xylosidase of Selenomonas ruminantium by site-directed mutagenesis of Trp145 -
Jordan, D.B., Wagschal, K.C., Zhanmin, F., Yuan, L., Braker, J.D., Heng, C. 2011. Engineering lower inhibitor affinities in beta-D-xylosidase of Selenomonas ruminantium by site-directed mutagenesis of Trp145. Journal of Industrial Microbiology and Biotechnology. 38:1821-1835.
Screening and characterization of hemicellulose-degrading enzymes -
Lee, C.C., Wagschal, K.C., Orts, W.J. Screening and characterization of hemicellulose-degrading enzymes. 2010 SIM Annual Meeting and Exhibition; Abstract S160b: p101.
and applications of microbial ß-D-xylosidases featuring the catalytically efficient enzyme from Selenomonas ruminantium -
Jordan, D.B., Wagschal, K.C. 2010. Properties and applications of microbial beta-D-xylosidases. Applied Microbiology and Biotechnology. 86(6):1647-1658
Cloning and characterization of alpha-glucuronidase enzymes from mixed cultures -
Lee, C.C., Kibblewhite, R.E., Wagschal, K.C., Orts, W.J. 2010. Cloning and characterization of alpha-glucuronidase enzymes from mixed cultures. Meeting Abstract. 85.
Cloning and characterization of alpha-glucuronidase enzyme -
Lee, C.C., Kibblewhite, R.E., Wagschal, K.C., Wong, D., Orts, W.J. 2010. Cloning and characterization of alpha-glucuronidase enzyme. Meeting Abstract.
Cloning and characterization of alpha-glucuronidase enzyme -
Lee, C.C., Kibblewhite, R.E., Wagschal, K.C., Wong, D., Orts, W.J. 2010. Cloning and characterization of alpha-glucuronidase enzyme. Meeting Abstract.
Chromosomal integration of recombinant alpha-amylase and glucoamylase genes in saccharomyces cerevisiae for starch conversion -
Wong, D., Batt Throne, S.B., Robertson, G.H., Lee, C.C., Wagschal, K.C. 2010. Chromosomal integration of recombinant alpha-amylase and glucoamylase genes in saccharomyces cerevisiae for starch conversion. Industrial Biotechnology. 6:112-118.
Engineering lower inhibitor affinities in beta-D-xylosidase by site-directed mutagenesis of Trp 145 -
Jordan, D.B., Wagschal, K.C., Fan, Z., Yuan, L., Braker, J.D., Heng, C. 2010. Engineering lower inhibitor affinities in beta-D-xylosidase by site-directed mutagenesis of Trp 145. Meeting Abstract. 84.
Engineering Lower Inhibitor Affinities in Beta-D-Xylosidase -
Fan, Z., Yuan, L., Jordan, D.B., Wagschal, K.C., Heng, C., Braker, J.D. 2010. Engineering lower inhibitor affinities in beta-D-xylosidase. Applied Microbiology and Biotechnology. 86(4):1099-1113.
Cloning and characterization of an alpha-glucuronidase from a mixed microbial population -
Lee, C.C., Kibblewhite, R.E., Wagschal, K.C., Robertson, G.H., Orts, W.J. 2009. Cloning and characterization of an alpha-glucuronidase from a mixed microbial population. Enzyme and Microbial Technology. 155(1-3):314-320.
Engineer Sccharomyces cerevisiae for consolidated bioprocessing -
Wong, D., Batt Throne, S.B., Wan, D., Lee, C.C., Wagschal, K.C. 2009. Engineer Sccharomyces cerevisiae for consolidated bioprocessing. Meeting Abstract.
Cloning biomass-degrading enzymes from environmental sources -
Lee, C.C., Wong, D., Wagschal, K.C., Orts, W.J. 2009. Cloning biomass-degrading enzymes from environmental sources. Meeting Abstract.
Purification and characterization of a glycoside hydrolase family 43 Beta-xylosidase from Geobacillus thermoleovorans IT-08 -
Wagschal, K.C., Heng, C., Lee, C.C., Robertson, G.H., Orts, W.J., Wong, D. 2008. Purification and characterization of a glycoside hydrolase family 43 Beta-xylosidase from Geobacillus thermoleovorans IT-08. Applied Biochemistry and Biotechnology. 155(1-3):1-10.
Biochemical characterization of a novel dual-function a-L-arabinofuranosidase/b-xylosidase isolated from a compost starter mixture -
Wagschal, K.C., Heng, C., Lee, C.C., Wong, D. 2009. Biochemical characterization of a novel dual-function a-L-arabinofuranosidase/b-xylosidase isolated from a compost starter mixture. Applied Microbiology and Biotechnology. 81:855-863.
Metagenomic gene discovery and enzyme engineering for biomass conversion -
Wong, D., Lee, C.C., Wagschal, K.C., Smith, M.R., Robertson, G.H., Orts, W.J. Metagenomic gene discovery and enzyme engineering for biomass conversion. American Chemical Society Western Regional Meeting. American Chemical Society Symposium: New Developments in Biofuels Research. Abstract 204.
Gene discovery and engineering for biomass degradation -
Lee, C.C., Wagschal, K.C., Wong, D. 2008. Gene discovery and engineering for biomass degradation. United States-Japan Cooperative Program in Natural Resources. 2008. 50-51.
Cloning of Bacillus licheniformis xylanase gene and characterization of recombinant enzyme -
Lee, C.C., Accinelli, R., Smith, M.R., Wagschal, K.C., Orts, W.J., Wong, D. 2008. Cloning of Bacillus licheniformis xylanase gene and characterization of recombinant enzyme. Current Microbiology. 57:301-305.
Biofuels Group NSF DUSEL Project -
Bang, S., Sani, R., Bleakly, B., Bischoff, K.M., Hughes, S.R., Wagschal, K.C., Wong, D., Lee, C.C., Jordan, D.B., Pokkuluri, R., Schiffer, M. 2008. Biofuels Group NSF DUSEL Project [abstract]. Homestake DUSEL Spring Workshop. Talk 10. p. 2.
Cloning, Expression and Characterization of a Glycoside Hydrolase Family 39 Xylosidase from Bacillus Halodurans C-125 -
Wagschal, K.C., Franquivillanueva, D.M., Lee, C.C., Robertson, G.H., Wong, D. Cloning, Expression and Characterization of a Glycoside Hydrolase Family 39 Xylosidase from Bacillus Halodurans C-125. Applied Biochemistry and Biotechnology.
Cloning and characterization of xylanase gene from hot spring microorganism -
Lee, C.C., Accinelli, R., Smith, M.R., Wagschal, K.C., Orts, W.J., Wong, D. Cloning and characterization of xylanase gene from hot spring microorganism. Meeting Abstract #307.
Developing enzyme systems for biomass deconstruction -
Wong, D., Lee, C.C., Wagschal, K.C., Smith, M.R., Robertson, G.H., Orts, W.J. 2007. Developing enzyme systems for biomass deconstruction. Meeting Abstract. Paper No. 217
SYNERGISTIC ACTION OF ALPHA-AMYLASE AND GLUCOAMYLASE ON THE HYDROLYSIS OF NATIVE STARCH GRANULES -
Wong, D., Robertson, G.H., Batt, S.B., Lee, C.C., Wagschal, K.C. 2007. Synergistic action of alpha-amylase and glucoamylase on the hydrolysis of native starch granules. Protein and Peptide Letters. 26:159-164.
EXPRESSION AND SECRETION OF CLOSTRIDIUM CELLULOVORANS XYNA IN SACCHAROMYCES CEREVISIAE -
Batt, S.B., Wong, D., Wagschal, K.C., Lee, C.C. 2006. Expression and secretion of clostridium cellulovorans xyna in saccharomyces cerevisiae. Division of Biological Chemistry, Enzymes Program. Abstract 220.
GENETIC AND BIOCHEMICAL CHARACTERIZATION OF A NOVEL BACTERIAL A-L-ARABINOFURANOSIDASE -
Wagschal, K.C., Franqui Espiet, D., Lee, C.C., Accinelli, R., Robertson, G.H., Wong, D. 2007. Genetic and biochemical characterization of a novel bacterial a-l-arabinofuranosidase. Enzyme and Microbial Technology. (40):747-753.
EVOLUTIONARY ENZYME DESIGN FOR IMPROVED BIOREFINING OF CROPS AND RESIDUES -
Wong, D., Lee, C.C., Wagschal, K.C., Smith, M.R., Robertson, G.H., Orts, W.J. 2006. Evolutionary enzyme design for improved biorefining of crops and residues. Technical Program (abstract). P8.
SYNERGISTIC ACTION OF BARLEY ALPHA-AMYLASE AND LENTINULA EDODES GLUCOAMYLASE ON RAW STARCH HYDROLYSIS -
Wong, D., Robertson, G.H., Lee, C.C., Wagschal, K.C. 2006. Synergistic action of barley alpha-amylase and lentinula edodes glucoamylase on raw starch hydrolysis. Approved 10/27/2005. American Society of Biochemistry & Molecular Biology, San Francisco, CA. April 1-4, 2006, Program 73.16, vol A27.
CLONING AND CHARACTERIZATION OF COLD-ACTIVE XYLANASE ENZYMES -
Lee, C.C., Accinelli, R., Wagschal, K.C., Robertson, G.H., Wong, D. 2006. Cloning and characterization of cold-active xylanase enzymes. 28th Symposium on Biotechnology for Fuels and Chemicals. Paper No. 1A-41.
CLONING AND CHARACTERIZATION OF A COLD ACTIVE XYLANASE ENZYME -
Lee, C.C., Accinelli, R., Wagschal, K.C., Robertson, G.H., Wong, D. 2006. Cloning and characterization of a cold active xylanase enzyme. Extremophiles. 10:295-300.
NATIVE OR RAW STARCH DIGESTION -
Robertson, G.H., Wong, D., Lee, C.C., Wagschal, K.C., Smith, M.R., Orts, W.J. 2006. Native or raw starch digestion: A Key step in energy efficient biorefining of grain. Journal of Agricultural and Food Chemistry, 54:353-65.
ISOLATION AND CHARACTERIZATION OF A PSYCHROPHILIC XYLANASE ENZYME FROM FLAVOBACTERIUM SP. -
Lee, C.C., Smith, M.R., Accinelli, R., Williams, T.G., Wagschal, K.C., Wong, D., Robertson, G.H. 2006. Isolation and characterization of a psychrophilic xylanase enzyme from flavobacterium sp. Current Microbiology, 52(2):112-116.
CHARACTERIZATION OF ACTIVE LENTINULA EDODES GLUCOAMYLASE EXPRESSED AND SECRETED BY SACCHAROMYCES CEREVISIAE -
Wong, D., Batt, S.B., Lee, C.C., Wagschal, K.C., Robertson, G.H. 2005. Characterization of active lentinula edodes glucoamylase expressed and secreted by saccharomyces cerevisiae. Protein Journal. vol 24, pp 455-463.
CHARACTERIZATION AND DIRECTED EVOLUTION OF HEMICELLULOTIC ENZYMES USING NATURAL XYLOOLIGOACCHARIDE SUBSTRATES -
Wagschal, K.C., Franqui Espiet, D., Lee, C.C., Robertson, G.H., Wong, D.W.S. 2005. Characterization of hemicellulotic degrading enzymes using natural substrates. Symposium on Biotechnology for Fuels and Chemicals, Denver, CO. Paper No. 1B-65. Abstract A10, Enzyme Engineering XVIII.
ENZYME-COUPLED ASSAY FOR BETA-XYLOSIDASE HYDROLYSIS OF NATURAL SUBSTRATES -
Wagschal, K.C., Franqui Espiet, D., Lee, C.C., Robertson, G.H., Wong, D. 2005. Enzyme-coupled assay for beta-xylosidase hydrolysis of natural substrates. Applied and Environmental Microbiology. 71:5318-5323.
EVOLVED ENZYMES FOR COLD OR NON-COOKING HYDROLYSIS OF STARCHES TO FERMENTATION FEEDSTOCK -
Robertson, G.H., Wong, D., Lee, C.C., Wagschal, K.C., Smith, M.R. 2005. Evolved enzymes for cold or non-cooking hydrolysis of starches to fermentation feedstock. In Proceedings of the North American Grain Congress, February 20-22, 2005, Reno, NV. 2005 CDROM.
EXPRESSION AND CHARACTERIZATION OF ACTIVE LENTINUS EDODES GLUCOAMYLASE IN SACCHAROMYCES CEREVISIAE -
Wong, D., Batt, S.B., Lee, C.C., Wagschal, K.C., Robertson, G.H. 2005. Expression and characterization of active. lentinus edodes glucoamylase in saccharomyces cerevisiae. Presentation 04:05. Paper No 11277.
CLONING AND CHARACTERIZATION OF BIOMASS DEGRADING ENZYMES -
Lee, C.C., Accinelli, R., Batt, S.B., Williams, T.G., Wong, D., Wagschal, K.C., Robertson, G.H. 2005. Cloning and characterization of biomass degrading enzymes. Symposium on Biotechnology for Fuels and Chemicals, Denver, CO. Paper No. IB-41 (Abstract).
DIRECTED EVOLUTION OF BARLEY ALPHA-AMYLASE FOR INCREASED ACTIVITY -
Batt, S.B., Wong, D., Lee, C.C., Wagschal, K.C., Robertson, G.H. 2004. Directed evolution of barley alpha-amylase for increased activity. American Chemical Society Western Regional Meeting, Sacramento, CA. Paper No. 144 (Abstracts).
HETEROGENEOUS BIOCATALYIC LIQUEFACTION AND SACCHARIFICAATION OF STARCH FOR ETHANOL -
Robertson, G.H., Wong, D., Lee, C.C., Wagschal, K.C. 2004. Heterogeneous biocatalyic liquefaction and saccharificaation of starch for ethanol. Corn Utilization Conference Proceedings, June 6-9, 2004, Indianapolis, Indiana, Abstract.
ARS News Articles
Last Modified: 5/2/2016
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