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United States Department of Agriculture

Agricultural Research Service

Related Topics

Jeffrey A Mertens
Bioenergy Research
General Biological Science

Phone: (309) 681-6086
Fax: (309) 681-6427
Room 2127A

1815 N UNIVERSITY ST
PEORIA , IL 61604

Projects
Technologies for Improving Process Efficiencies in Biomass Refineries
Appropriated (D)
  Accession Number: 427437

Publications (Clicking on the reprint icon Reprint Icon will take you to the publication reprint.)
Engineering increased thermostability in the GH-10 endo-1,4-ß-xylanase from Thermoascus aurantiacus CBMAI 756 -
De Souza, A.R., De Araujo, G.C., Zanphorlin, L.M., Ruller, R., Franco, F.C., Torres, F.A.G., Mertens, J.A., Bowman, M.J., Gomes, E., Da Silva, R. 2016. Engineering increased thermostability in the GH-10 endo-1,4-ß-xylanase from Thermoascus aurantiacus CBMAI 756. International Journal of Biological Macromolecules. 93:20-26. doi: 10.1016/j.ijbiomac.2016.08.056.
Kinetic properties of Rhizopus oryzae RPG1 endo-polygalacturonase hydrolyzing galacturonic acid oligomers Reprint Icon -
Mertens, J.A., Bowman, M.J. 2016. Kinetic properties of Rhizopus oryzae RPG1 endo-polygalacturonase hydrolyzing galacturonic acid oligomers. Biocatalysis and Agricultural Biotechnology. 5:11-16. doi: 10.1016/j.bcab.2015.12.005.
Triacetic acid lactone production in industrial Saccharomyces yeast strains Reprint Icon -
Saunders, L.P., Bowman, M.J., Mertens, J.A., Da Silva, N.A., Hector, R.E. 2015. Triacetic acid lactone production in industrial Saccharomyces yeast strains. Journal of Industrial Microbiology and Biotechnology. 42:711-721.
Isolation and characterization of unhydrolyzed oligosaccharides from switchgrass (Panicum virgatum, L.) xylan after exhaustive enzymatic treatment with commercial enzyme preparations Reprint Icon -
Bowman, M.J., Dien, B.S., Vermillion, K.E., Mertens, J.A. 2015. Isolation and characterization of unhydrolyzed oligosaccharides from switchgrass (Panicum virgatum, L.) xylan after exhaustive enzymatic treatment with commercial enzyme preparations. Carbohydrate Research. 407:42-50.
Structural characterization (1->2)-beta-xylose-(1->3)-alpha-arabinose-containing oligosaccharide products of extracted switchgrass (Panicum virgatum, L.) xylan treatment with alpha-arabinofuranosidase and beta-endo-xylanase. Reprint Icon -
Bowman, M.J., Dien, B.S., Vermillion, K., Mertens, J.A. 2014. Structural characterization of (1-2)-ß-xylose-(1-3)-alpha-arabinose-containing oligosaccharide products of extracted switchgrass (Panicum virgatum, L.) xylan after exhaustive enzymatic treatment with alpha-arabinofuranosidase and ß-endo-xylanase. Carbohydrate Research. 398:63-71.
Kinetic characteristics of polygalacturonase enzymes hydrolyzing galacturonic acid oligomers using isothermal titration calorimetry -
Mertens, J.A. 2013. Kinetic characteristics of polygalacturonase enzymes hydrolyzing galacturonic acid oligomers using isothermal titration calorimetry [abstract]. Society of Industrial Microbiology. p. 90.
Kinetic properties of two Rhizopus exo-polygalacturonase enzymes hydrolyzing galacturonic acid oligomers using isothermal titration calorimetry -
Mertens, J.A. 2013. Kinetic properties of two Rhizopus exo-polygalacturonase enzymes hydrolyzing galacturonic acid oligomers using isothermal titration calorimetry. Applied Biochemistry and Biotechnology. 170(8):2009-2020.
Growth and fermentation of D-xylose by Saccharomyces cerevisiae expressing a novel D-xylose isomerase originating from the bacterium Prevotella ruminicola TC2-24 Reprint Icon -
Hector, R.E., Dien, B.S., Cotta, M.A., Mertens, J.A. 2013. Growth and fermentation of D-xylose by Saccharomyces cerevisiae expressing a novel D-xylose isomerase originating from the bacterium Prevotella ruminicola TC2-24. Biotechnology for Biofuels. 6:84.
Investigation of accessory hemicellulases and pectinases for polysaccharide hydrolysis of ionic liquid pretreated biomass -
Barr, C.J., Mertens, J.A., Schall, C. 2012. Investigation of accessory hemicellulases and pectinases for polysaccharide hydrolysis of ionic liquid pretreated biomass [abstract]. American Institute of Chemical Engineers (AIChE).
The ultrasound-enhanced bioscouring performance of four polygalacturonase enzymes obtained from rhizopus oryzae -
Easson, M.W., Mertens, J.A., Fortier, C.A., Slopek, R.P., Condon, B.D., Boykin, D.L., Tarver, M.R. 2013. The ultrasound-enhanced bioscouring performance of four polygalacturonase enzymes obtained from rhizopus oryzae. American Association of Textile Chemists and Colorists Review. 13(2):51-56.
Plant cell walls to ethanol. -
Jordan, D.B., Bowman, M.J., Braker, J.D., Dien, B.S., Hector, R.E., Lee, C.C., Mertens, J.A., Wagschal, K.C. 2012. Plant cell walls to ethanol. Biochemical Journal. 442:247-252.
Critical cellulase and hemicellulase activities for hydrolysis of ionic liquid pretreated biomass -
Barr, C.J., Mertens, J.A., Schall, C.A. 2012. Critical cellulase and hemicellulase activities for hydrolysis of ionic liquid pretreated biomass. Bioresource Technology. 104:480-485.
Subsite binding energies of an exo-polygalacturonase using isothermal titration calorimetry -
Mertens, J.A., Hector, R.E., Bowman, M.J. 2012. Subsite binding energies of an exo-polygalacturonase using isothermal titration calorimetry. Thermochimica Acta. 527:219-222.
Biochemical processing of reed canarygrass into fuel ethanol -
Dien, B.S., Casler, M.D., Hector, R.E., Iten, L.B., Nichols, N.N., Mertens, J.A., Cotta, M.A. 2012. Biochemical processing of reed canarygrass into fuel ethanol. International Journal of Low-Carbon Technologies. 7:338-347.
The bioscouring performance of four polygalacturonase enzymes -
Easson, M.W., Mertens, J.A., Boykin, D.L., Fortier, C.A., Tarver, M.R., Condon, B.D. 2012. The bioscouring performance of four polygalacturonase enzymes. Proceedings of The American Association Of Textile Chemists And Colorists. 54-60.
Saccharomyces cerevisiae engineered for xylose metabolism requires gluconeogenesis and the oxidative branch of the pentose phosphate pathway for aerobic xylose assimilation Reprint Icon -
Hector, R.E., Mertens, J.A., Bowman, M.J., Nichols, N.N., Cotta, M.A., Hughes, S.R. 2011. Saccharomyces cerevisiae engineered for xylose metabolism requires gluconeogenesis and the oxidative branch of the pentose phosphate pathway for aerobic xylose assimilation. Yeast. 28:645-660.
Deconstruction of ionic liquid pretreated lignocellulosic biomass using mono-component cellulases and hemicellulases and commercial mixtures -
Expression and characterization of fifteen Rhizopus oryzae 99-880 polygalacturonase enzymes in Pichia pastoris -
Mertens, J.A., Bowman, M.J. 2011. Expression and characterization of fifteen Rhizopus oryzae 99-880 polygalacturonase enzymes in Pichia pastoris. Current Microbiology. 62(4):1173-1178.
Enzyme Systems for Optimization of Ionic-Liquid Pretreated Biomass Saccharification -
Barr, C.J., Wissinger, J., Samayam, I.P., Schall, C.A., Mertens, J.A. 2010. Enzyme Systems for Optimization of Ionic-Liquid Pretreated Biomass Saccharification [abstract]. American Institute of Chemical Engineers. p. 265.
Catalytic properties of two Rhizopus oryzae 99-880 glucoamylase enzymes without starch binding domains expressed in Pichia pastoris -
Mertens, J.A., Braker, J.D., Jordan, D.B. 2010. Catalytic properties of two Rhizopus oryzae 99-880 glucoamylase enzymes without starch binding domains expressed in Pichia pastoris. Applied Biochemistry and Biotechnology. 162(8):2197-2213.
Enzyme Characterization of Cellulase and Hemicellulases Component Enzymes and Saccharification of Ionic Liquid Pretreated Lignocellulosic Biomass -
Barr, C.J., Schall, C.A., Mertens, J.A. 2010. Enzyme Characterization of Cellulase and Hemicellulases Component Enzymes and Saccharification of Ionic Liquid Pretreated Lignocellulosic Biomass [abstract]. Biotechnology for Fuels and Chemicals. p. 85.
Identification, Characterization, and Evolution of a Large Polygalacturonase Gene Family from Rhizopus oryzae -
Inhibition of Rhizopus Lactate Dehydrogenase by Fructose 1,6-bisphosphate -
Skory, C.D., Mertens, J.A., Rich, J.O. 2009. Inhibition of Rhizopus lactate dehydrogenase by fructose 1,6-bisphosphate. Enzyme and Microbial Technology. 44(4):242-247.
Identification, Biochemical Characterization, and Evolution of the Rhizopus oryzae 99-880 Polygalacturonase Gene Family -
Mertens, J.A., Burdick, R.C., Rooney, A.P. 2008. Identification, Biochemical Characterization, and Evolution of the Rhizopus oryzae 99-880 Polygalacturonase Gene Family. Fungal Genetics and Biology. 45(12):1616-1624.
Aminoalcohols as Probes of the Two-subsite Active Site of Beta-D-xylosidase from Selenomonas ruminantium -
Jordan, D.B., Mertens, J.A., Braker, J.D. 2009. Aminoalcohols as Probes of the Two-subsite Active Site of Beta-D-xylosidase from Selenomonas ruminantium. Biochimica et Biophysica Acta. 1794(1):144-158.
Engineered Saccharomyces cerevisiae strain for improved xylose utilization with a three-plasmid SUMO yeast expression system -
Hughes, S.R., Sterner, D.E., Bischoff, K.M., Hector, R.E., Dowd, P.F., Qureshi, N., Bang, S.S., Grynavyski, N., Chakrabarty, T., Johnson, E.T., Dien, B.S., Mertens, J.A., Caughey, R.J., Liu, S., Butt, T.R., Labaer, J., Cotta, M.A., Rich, J.O. 2009. Engineered Saccharomyces cerevisiae strain for improved xylose utilization with a three-plasmid SUMO yeast expression system. Plasmid Journal. 61(1):22-38.
Isolation and characterization of 17 different genes encoding putative endopolygalacturonase genes from Rhizopus oryzae -
Mertens, J.A., Burdick, R.C. 2008. Isolation and characterization of 17 different genes encoding putative endopolygalacturonase genes from Rhizopus oryzae [abstract]. Society of Industrial Microbiology. p. 116.
Identification and transcriptional profiling of Pseudomonas putida genes involved in furoic acid metabolism -
Nichols, N.N., Mertens, J.A. 2008. Identification and transcriptional profiling of Pseudomonas putida genes involved in furoic acid metabolism. FEMS Microbiology Letters. 284:52-57.
Lycotoxin-1 insecticidal peptide optimized by amino acid scanning mutagenesis and expressed as a co-product in an ethanologenix Saccharomyces cerevisiae strain -
Hughes, S.R., Dowd, P.F., Hector, R.E., Panavas, T., Sterner, D.E., Qureshi, N., Bischoff, K.M., Bang, S.B., Mertens, J.A., Johnson, E.T., Li, X., Jackson Jr, J.S., Caughey, R.J., Riedmuller, S.B., Bartolett, S., Liu, S., Rich, J.O., Farrelly, P.J., Butt, T.R., Labaer, J., Cotta, M.A. 2008. Lycotoxin-1 insecticidal peptide optimized by amino acid scanning mutagenesis and expressed as a co-product in an ethanologenix Saccharomyces cerevisiae strain. Journal of Peptide Science. 14(9):1039-1050. Available: http://www3.interscience.wiley.com/cgi-bin/fulltext/119030240/PDFSTART.
Molecular cloning and expression of three polygalacturonase cDNAs from the tarnished plant bug, Lygus lineolaris Reprint Icon -
Allen, M.L., Mertens, J.A. 2008. Molecular cloning and expression of three polygalacturonase cDNAs from the tarnished plant bug, Lygus lineolaris. J. Insect Sci. 8 Article 27:1-14. 2008.
High-Throughput Fully Automated Construction of a Multiplex Library of Mutagenized Open Reading Frames for an Insecticidal Peptide Using a Plasmid-Based Functional Proteomic Robotic Workcell with Improved Vacuum System -
Hughes, S.R., Dowd, P.F., Hector, R.E., Riedmuller, S.B., Bartolett, S., Mertens, J.A., Qureshi, N., Liu, S., Bischoff, K.M., Li, X., Jackson Jr, J.S., Sterner, D., Panavas, T., Cotta, M.A., Farrelly, P.J., Butt, T. 2007. High-throughput fully automated construction of a multiplex library of mutagenized open reading frames for an insecticidal peptide using a plasmid-based functional proteomic robotic workcell with improved vacuum system. Journal of Laboratory Automation. 12(4):202-212.
Fully automated molecular biology routines on a plasmid-based functional proteomic workcell: Evaluation and Characterization of Yeast Strains Optimized for Growth on Xylose Expressing "Stealth" Insecticidal Peptides. -
Hughes, S.R., Li, X., Hector, R.E., Bischoff, K.M., Cotta, M.A., Mertens, J.A., Dowd, P.F., Johnson, E.T. 2007. Fully automated molecular biology routines on a plasmid-based functional proteomic workcell: Evaluation and characterization of yeast strains optimized for growth on xylose expressing "stealth" insecticidal peptides [abstract]. American Chemical Society. p. 108.
Plasmid-Based Functional Proteomic Workcell Evaluation and Characterization of Yeast Strains with Improved Growth on Xylose Expressing Xylanase A(Xyn A) and Celulase F(Cel F) from Anaerobic Fungi Orpinomyces -
Hughes, S.R., Mertens, J.A., Qureshi, N., Cotta, M.A., Bischoff, K.M., Liu, S., Li, X. 2007. Plasmid-based functional proteomic workcell evaluation and characterization of yeast strains with improved growth on xylose expressing Xylanase A(Xyn A) and Celulase F(Cel F) from anaerobic fungi Orpinomyces [abstract]. World Congress on Industrial Biotechnology and Bioprocessing. Seminar 2. p. 19.
ISOLATION AND CHARACTERIZATION OF TWO GENES THAT ENCODE ACTIVE GLUCOAMYLASE WITHOUT A STARCH BINDING DOMAIN FROM A TYPE II RHIZOPUS ORYZAE -
Mertens, J.A., Skory, C.D. 2007. Isolation and characterization of two genes that encode active glucoamylase without a starch binding domain from Rhizopus oryzae. Current Microbiology. 54:462-466.
Fully automated molecular biology: Plasmid-Based Functional Proteomic Workcell Evaluation and Characterization of Yeast Strains with Optimized "Trojan Horse" Amino Acid Scanning Mutational Inserts. -
Hughes, S.R., Qureshi, N., Mertens, J.A., Cotta, M.A., Hector, R.E., Bischoff, K.M., Liu, S., Li, X-L., 2007. Fully automated molecular biology: Plasmid-based functional proteomic workcell evaluation and characterization of yeast strains with optimized "Trojan Horse" amino acid scanning mutational inserts [abstract]. PEPTALK 2007. Cambridge Healthtech Institute. Poster 509, p. 4.
Fully Automated Molecular Biology Routines on a Plasmid-Based Functional Proteomic Workcell: Evaluation and Characterization of Yeast Strains Optimized for Growth on Xylose and Engineered to Express an Insecticidal Peptide -
Hughes, S.R., Li, X., Liu, S., Bischoff, K.M., Mertens, J.A., Qureshi, N., Riedmuller, S., Bartolett, S., Farrelly, P., Panavas, T., Butt, T., Cotta, M.A. 2007. Fully automated molecular biology routines on a plasmid-based functional proteomic workcell: Evaluation and characterization of yeast strains optimized for growth on xylose and engineered to express an insecticidal peptide [abstract]. PEPTALK 2007, Cambridge Healthtech Institute. Talk 83, p. 7.
ISOLATION AND CHARACTERIZATION OF A SECOND GLUCOAMYLASE WITHOUT A STARCH BINDING DOMAIN FROM RHIZOPUS ORYZAE -
Mertens, J.A., Skory, C.D. 2007. Isolation and characterization of a second glucoamylase gene without a starch binding domain from Rhizopus oryzae. Enzyme and Microbial Technology. 40:874-880.
IDENTIFICATION AND TRANSCRIPTIONAL PROFILING OF FUROIC ACID METABOLISM GENES IN PSEUDOMONAS PUTIDA -
Nichols, N.N., Mertens, J.A., Dien, B.S. 2006. Identification and transcriptional profiling of furoic acid metabolism genes in Pseudomonas putida [abstract]. American Society for Microbiology. Paper No. Q-449.
PLASMIDS FOR EXPRESSION OF HETEROLOGOUS PROTEINS IN RHIZOPUS ORYZAE -
Mertens, J.A., Skory, C.D., Ibrahim, A.S. 2006. Plasmids for expression of heterologous proteins in Rhizopus oryzae. Archives of Microbiology. 186:41-50.
FUNCTIONAL PROTEOMIC PLASMID-BASED INTEGRATED WORKCELL FOR HIGH-THROUGHPUT TRANSFORMATION OF BL21 DE3 E. COLI FOR EXPRESSION IN VIVO WITH PIROMYCES STRAIN XYLOSE ISOMERASE -
Hughes, S.R., Riedmuller, S.B., Mertens, J.A., Li, X., Bischoff, K.M., Liu, S., Qureshi, N., Cotta, M.A., Skory, C.D., Gorsich, S.W., Farrelly, P.J. 2006. Functional proteomic plasmid-based integrated workcell for high-throughput transformation of BL21 DE3 E. coli for expression in vivo with piromyces strain xylose isomerase [abstract]. Midwest Laboratory Robotics Information Group. p. 2.
HIGH-THROUGHPUT SCREENING OF CELLULASE F MUTANTS FROM MULTIPLEXED PLASMID SETS USING AN AUTOMATED PLATE ASSAY ON A FUNCTIONAL PROTEOMIC ROBOTIC WORKCELL -
Hughes, S.R., Riedmuller, S.B., Mertens, J.A., Li, X., Bischoff, K.M., Qureshi, N., Cotta, M.A., Farrelly, P.J. 2006. High-throughput screening of cellulase F mutants from multiplexed plasmid sets using an automated plate assay on a functional proteomic robotic workcell. Proteome Science. 4:10.
AUTOMATED STRATEGY USING A FUNCTIONAL PROTEOMIC ASSAY TO IDENTIFY AND ISOLATE CELLULASE F MUTANTS WITH IMPROVED ACTIVITY FROM MULTIPLEXED SETS OF PLASMID -
Hughes, S.R., Riedmuller, S., Mertens, J.A., Li, X., Qureshi, N., Farrelly, P., Cotta, M.A. 2006. Automated strategy using a functional proteomic assay to identify and isolate cellulase F mutants with improved activity from multiplexed sets of plasmid [abstract]. PepTalk 2006. p. 10.
PLASMID-BASED FUNCTIONAL PROTEOMIC ROBOTIC WORKCELL PROCESS FOR HIGH-THROUGHPUT SCREENING OF MULTIPLEXED LIBRARIES OF MUTAGENIZED CLONES -
Hughes, S.R., Mertens, J.A., Li, X., Bischoff, K.M. 2005. Plasmid-based functional proteomic robotic workcell process for high-throughput screening of multiplexed libraries of mutagenized clones [abstract]. Laboratory Robotics Information Group. p. 1.
PLASMID-BASED FUNCTIONAL PROTEOMIC ROBOTIC WORKCELL PROCESS FOR HIGH-THROUGHPUT SCREENING OF MULTIPLEXED LIBRARIES OF MUTAGENIZED CLONES -
Hughes, S.R., Riedmuller, S., Mertens, J.A., Li, X., Qureshi, N., Bischoff, K.M., Jordan, D.B., Cotta, M.A., Farrelly, P. 2005. Plasmid-based functional proteomic robotic workcell process for high-throughput screening of multiplexed libraries of mutagenized clones [abstract]. Optimization high-throughput Cultures for Bioprocessing 2005. p. 3.
FUNCTIONAL PROTEOMIC WORKCELL FOR HIGH VOLUME PLASMID PREPARATIONS FOR REPEATED IN VITRO PROTEIN EXPRESSION AND HIGH THROUGHPUT SCREENING TO IDENTIFY MUTANT ENYZMES FOR USE AT LOW PH -
Hughes, S.R., Riedmuller, S.B., Mertens, J.A., Jordan, D.B., Li, X., Qureshi, N., Cotta, M.A., Farrelly, P.J., Bischoff, K.M. 2005. Functional proteomic workcell for high volume plasmid preparations for repeated in vitro protein expression and high throughput screening to identify mutant enyzmes for use at low pH [abstract]. Optimization High-throughput Cultures for Bioprocessing 2005. 13:3.
DEVELOPMENT OF PLASMIDS FOR EXPRESSION OF HETEROLOGOUS PROTEINS IN RHIZOPUS ORYZAE -
Mertens, J.A., Skory, C.D. 2005. Development of plasmids for expression of heterologous proteins in Rhizopus oryzae [abstract]. Society of Industrial Microbiology. p. 100.
DEVELOPMENT OF A LIQUID HANDLER COMPONENT FOR A PLASMID-BASED FUNCTIONAL PROTEOMIC ROBOTIC WORKCELL -
Hughes, S.R., Riedmuller, S., Mertens, J.A., Li, X., Bischoff, K.M., Cotta, M.A., Farrelly, P. 2005. Development of a liquid handler component for a plasmid-based functional proteomic robotic workcell. Journal of the Association for Laboratory Automation. 10(5):287-300.
DEVELOPMENT OF A LIQUID HANDLER COMPONENT FOR A FUNCTIONAL PLASMID-BASED PROTEOMIC WORKCELL THAT GENERATES MULTIPLEX SAMPLES EXPRESSES IN YEAST -
Hughes, S.R., Riedmuller, S.B., Bischoff, K.M., Mertens, J.A., Li, X., Cotta, M.A., Farrelly, P.J. 2005. Development of a liquid handler component for a functional plasmid-based proteomic workcell that generates multiplex samples expresses in yeast [abstract]. Association for Laboratory Automation, LabAutomation 2005. Poster WP128.
A PLASMID-BASED FUNCTIONAL PROTEOMIC WORKCELL USED TO OPTIMIZE YEAST FOR FUEL ETHANOL PRODUCTION -
Hughes, S.R., Li, X., Cotta, M.A., Mertens, J.A., Bischoff, K.M., Riedmuller, S., Farrelly, P., Patel, M., Brown, L., Carter, L. 2005. A plasmid-based functional proteomic workcell used to optimize yeast for fuel ethanol production [abstract]. CHI's Annual PEP Talk Meeting, January 10-14, 2005, San Diego, California. p. 3.
MODIFIED LIQUID HANDLER USED TO PRODUCE HIGH THROUGHPUT HIGH QUALITY PLASMIDS FOR INTEGRATION INTO PLASMID-BASED FUNCTIONAL PROTEOMICS WORKCELL -
Hughes, S.R., Li, X., Cotta, M.A., Mertens, J.A., Bischoff, K.M., Riedmuller, S., Farrelly, P., Patel, M., Brown, L., Carter, L. 2005. Modified liquid handler used to produce high throughput high quality plasmids for integration into plasmid-based functional proteomics workcell [abstract]. CHI's Annual PEP Talk Meeting, January 10-14, 2005, San Diego, California. Poster 151.
DISCOVERY AND CHARACTERIZATION OF A SECOND GLUCOAMYLASE GENE IN RHIZOPUS ORYZAE -
Mertens, J.A., Skory, C.D. 2004. Discovery and characterization of a second glucoamylase gene in rhizopus oryzae [abstract]. Society of Industrial Microbiology. p. 96.
Last Modified: 5/2/2016
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