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David Matthew Donovan
Animal Biosciences & Biotechnology Laboratory
Research Molecular Biologist

Phone: (301) 504-9150
Fax: (301) 504-8571
Room 104

10300 BALTIMORE AVENUE
BLDG 230 BARC-EAST
BELTSVILLE , MD 20705

Publications (Clicking on the reprint icon Reprint Icon will take you to the publication reprint.)
Antimicrobial activity of bacteriophage derived triple fusion protein against Staphylococcus aureus -
Kovalskaya, N.Y., Herndon, E., Foster Frey, J.A., Donovan, D.M., Hammond, R. 2019. Antimicrobial activity of bacteriophage derived triple fusion protein against Staphylococcus aureus. AIMS Microbiology. https://doi.org/10.3934/microbiol.2019.2.158.
Genetic basis for the resistance of Staphylococcus aureus to peptidoglycan hydrolase by comparative transcriptome and whole genome sequence analysis -
Bovine trophectoderm cell lines induced from bovine fibroblasts with reprogramming factors -
Donovan, D.M., Ealy, A.D., Powell, A.M., Caperna, T.J., Blomberg, L., Garrett, W.M., Sparks, W.O., Talbot, N.C. 2017. Bovine trophectoderm cell lines induced from bovine fibroblasts with reprogramming factors. Reproduction. 84(6):468-485.
Identification of peptidoglycan hydrolase constructs with synergistic staphylolytic activity in cow's milk -
Verbree, C.T., Datwyler, S.M., Eichenseher, F., Meile, S., Donovan, D.M., Loessner, M.J., Schmelcher, M. 2017. Identification of peptidoglycan hydrolase constructs with synergistic staphylolytic activity in cow's milk. Applied and Environmental Microbiology. https://doi.org/10.1128/AEM.03445-16.
Targeted gene knock-in by CRISPR/Cas ribonucleoproteins in porcine zygotes -
Park, K., Powell, A.M., Sandmaier, S.E., Kim, C., Mileham, A., Donovan, D.M., Telugu, B.P. 2017. Targeted gene knock-in by CRISPR/Cas ribonucleoproteins in porcine zygotes. Stem Cells and Development. doi: 10.1038/srep42458.
Production of germline ablated male pigs via Crispr/Cas editing of the NANOS2 gene -
Kaucher, A., Park, K., Powell, A.M., Oatley, M., Donovan, D.M., Blomberg, L., Lillico, S., Whitlaw, B., Mileham, A., Telugu, B., Oatley, J. 2017. Production of germline ablated male pigs via Crispr/Cas editing of the NANOS2 gene. Meeting Abstract. 10;7:40176. doi: 10.1038/srep40176..
Novel antimicrobial compounds from microbial sources -
Bischoff, K.M., Leathers, T.D., Price, N.P., Skory, C.D., Liu, S., Donovan, D.M., Rich, J.O. 2016. Novel antimicrobial compounds from microbial sources [abstract]. Alternative to Antibiotics.
Characterization of a novel Streptococcus suis endolysin and development of a multi-acting antimicrobial enzyme that is refractory to resistance development -
Generation of germline ablated male pigs by CRISPR/Cas9 editing of the NANOS2 gene -
Ki-Eun, P., Kaucher, A., Powell, A.M., Wagas, M., Sandmaier, S., Oatley, M.J., Park, C., Tibary, A., Donovan, D.M., Blomberg, L., Lillico, S., Whitelaw, B., Mileham, A., Telugu, B., Oatley, J.M. 2017. Generation of germline ablated male pigs by CRISPR/Cas9 editing of the NANOS2 gene. Nature Communications. doi: 10.1038/srep40176.
Somatic cell nuclear transfer followed by CRIPSR/CAS9 microinjection results in highly efficient genome editing in cloned pigs -
Sheets, T.P., Park, C., Park, K., Powell, A.M., Donovan, D.M., Telugu, B.P. 2016. Somatic cell nuclear transfer followed by CRIPSR/CAS9 microinjection results in highly efficient genome editing in cloned pigs. International Journal of Molecular Sciences. doi: 10.3390/ijms17122031.
Characterization of the endolysin from the Enterococcus faecalis bacteriophage VD13 -
Swift, S., Rowley, D.T., Young, C., Franks, A., Hyman, P., Donovan, D.M. 2016. Characterization of the endolysin from the Enterococcus faecalis bacteriophage VD13. FEMS Microbiology Letters. doi: 10.1093/femsle/fnw216.
Engineering genomes of domestic pigs for agricultural applications -
Four phage endolysins that are lytic for clostridium perfringens -
Targeted gene knockin in porcine somatic cells using CRISPR/Cas ribonucleoproteins -
Park, K., Park, C., Powell, A.M., Donovan, D.M., Telugu, B.P. 2016. Targeted gene knockin in porcine somatic cells using CRISPR/Cas ribonucleoproteins. International Journal of Molecular Sciences. 17(6).
Triple-acting lytic enzyme treatment of drug-resistant and intracellular Staphylococcus aureus -
Becker, S.C., Roach, D.R., Chauhan, V., Shen, Y., Foster Frey, J.A., Powell, A.M., Buchannan, G., Lease, R., Mohammadi, H., Harty, W.J., Simmons, C.B., Schmelcher, M., Camp, M.J., Shields, K., Dowd, M., Dong, S., Baker, J.R., Sheen, T.R., Doran, K.S., Pritchard, D.G., Almeida, R., Nelson, D.C., Marriott, I., Lee, J.C., Donovan, D.M. 2016. Triple-acting lytic enzyme treatment of drug-resistant and intracellular Staphylococcus aureus. Scientific Reports. 6:25063.
Evaluation of a lysostaphin-fusion protein as a dry-cow therapy for Staphylococcus aureus mastitis in dairy cattle -
Hoernig, K., Pithua, P., Williams, Iii, F., Donovan, D.M., Middleton, J. 2016. Evaluation of a lysostaphin-fusion protein as a dry-cow therapy for Staphylococcus aureus mastitis in dairy cattle. Journal Dairy Science Supplement. 99(6):4638-46.
mRNA levels of imprinted genes in bovine in vivo oocytes, embryos and cross species comparisons in humans, mice and pigs -
Jiang, Z., Hong, D., Zheng, X., Donovan, D.M., Chen, J., Tian, X. 2015. mRNA levels of imprinted genes in bovine in vivo oocytes, embryos and cross species comparisons in humans, mice and pigs. Nature. 5:17898. doi: 10.1038/srep17898.
A chimeric LysK-lysostaphin fusion enzyme lysing Staphylococcus aureus cells: a study of both kinetics of inactivation and specifics of interaction with anionic polymers -
Filatova, L.Y., Donovan, D.M., Ishnazarova, N., Foster Frey, J.A., Becker, S.C., Pugachev, V.G., Dmitrieva, N.F., Klyachko, N.L. 2016. A chimeric LysK-lysostaphin fusion enzyme lysing Staphylococcus aureus cells: A study of both kinetics of inactivation and specifics of interaction with anionic polymers. Enzyme and Microbial Technology. doi: 10.1007/s12010-016-2115-7.
PlyC, a bacteriophage endolysin that is internalized by epithelial cells and retains bacteriolytic activity against intracellular streptococci -
Shen, Y., Barros, M., Vennemann, T., Gallagher, D., Yin, Y., Linden, S.B., Heselpoth, R.D., Spencer, D.J., Donovan, D.M., Moult, J., Fischetti, V.I., Heinrich, F., Losche, M., Nelson, D.C. 2016. PlyC, a bacteriophage endolysin that is internalized by epithelial cells and retains bacteriolytic activity against intracellular streptococci. Stem Cells and Development. doi: 10.7554/eLife.13152.
Expression of a bioactive bacteriophage endolysin in Nicotiana benthamiana plants -
Kovalskaya, N.Y., Foster Frey, J.A., Donovan, D.M., Bauchan, G.R., Hammond, R. 2015. Expression of a bioactive bacteriophage endolysin in Nicotiana benthamiana plants. Journal of Microbiology and Biotechnology. 26:160-170.
An antimicrobial peptidoglycan hydrolase for treating Enterococcus faecalis -
Fusion of a thermophilic phage endolysin to a Clostridium perfringens-specific cell wall binding domain creates an anti-clostridium antimicrobial with improved thermostability -
Knockdown Brm and Baf170, components of chromatin remodeling complex, facilitates reprogramming of somatic cells -
Jiang, Z., Tang, Y., Zhao, X., Donovan, D.M., Tian, X. 2015. Knockdown Brm and Baf170, components of chromatin remodeling complex, facilitates reprogramming of somatic cells. Stem Cells and Development. 24(19):2328-36.
Antimicrobial bacteriophage-derived proteins and therapeutic applications -
Roach, D.R., Donovan, D.M. 2015. Antimicrobial bacteriophage-derived proteins and therapeutic applications. Bacteriophage. Issue 3. DOI: 10.1080/21597081.2015.1062590.
Managing the gut microbiome of food animals with tools of microbial ecology -
A thermophilic phage endolysin fusion to a Clostridium perfringens-specific cell wall binding domain creates an anti-clostridium antimicrobial with improved thermostability -
Swift, S., Seal, B.S., Garrish, J.K., Oakley, B., Yeh, H., Woolsey, R., Schegg, K.M., Line, J.E., Donovan, D.M., Hiett, K.L. 2015. A thermophilic phage endolysin fusion to a Clostridium perfringens-specific cell wall binding domain creates an anti-clostridium antimicrobial with improved thermostability. Viruses and Bacteriophages. 7(6):3019-3034.
A thermophilic phage endolysin fusion to a Clostridium perfringens-specific cell wall binding domain creates an anti-clostridium antimicrobial with improved thermostability -
Swift, S., Seal, B.S., Garrish, J.K., Oakley, B., Yeh, H., Heitt, K., Woolsey, R., Schegg, K.M., Line, J.E., Donovan, D.M. 2015. A thermophilic phage endolysin fusion to a Clostridium perfringens-specific cell wall binding domain creates an anti-clostridium antimicrobial with improved thermostability. Meeting Abstract. 12;7(6):3019-34. doi: 10.3390/v7062758.
Application of CRISPR technology in genome editing in agriculture -swine -
Generation of induced Pluripotent Stem Cells from Domestic Goats - Capra hircus -
Sandmaier, S.E., Nandal, A., Powell, A.M., Garrett, W.M., Blomberg, L., Donovan, D.M., Talbot, N.C., Telugu, B.V. 2015. Generation of induced Pluripotent Stem Cells from Domestic Goats - Capra hircus. Molecular Reproduction and Development. 82:709–721.
Managing the gut microbiome of food animals with tools of microbial ecology -
Oakley, B., Kogut, M.H., Donovan, D.M., Cox Jr, N.A., Berrang, M.E., Chalghoumi, R., Seal, B. 2015. Managing the gut microbiome of food animals with tools of microbial ecology [abstract]. International Scientific Conference on Probiotics and Prebiotics. June 23-25, 2015. Budapest, Hungary.
An antimicrobial peptidoglycan hydrolase for treating Enterococcus faecalis -
Application of CRISPR technology in genome editing in agriculture -swine -
Discovery of the porcine NGN3 gene and testing its endocrine function in the pig -
Bacteriophage phi11 lysin: physicochemical characterization and comparison with phage phi80a lysin -
Filatova, L.Y., Donovan, D.M., Foster Frey, J.A., Pugachev, V.G., Dmitrieva, N.F., Chubar, T.A., Klyachko, N.L., Kabanov, A.V. 2015. Bacteriophage phi11 lysin: physicochemical characterization and comparison with phage phi80a lysin. Enzyme and Microbial Technology. 73-74:51-58. DOI: 10.1016/j.enzmictec.2015.03.005.
The streptococcal phage SA2 and B30 endolysins act synergistically and kill mastitis causing streptococci in milk -
Schmelcher, M., Pohl, C.S., Donovan, D.M. 2015. The streptococcal phage SA2 and B30 endolysins act synergistically and kill mastitis causing streptococci in milk. Applied and Environmental Microbiology. 99(20):8475-86.
Evolutionarily distinct bacteriophage endolysins featuring conserved peptidoglycan cleavage sites protect mice from MRSA infection -
Schmelcher, M., Shen, Y., Nelson, D.C., Eugster, M.R., Eichenseher, F., Hanke, D.C., Loessner, M.J., Dong, S., Pritchard, D.G., Lee, J.C., Becker, S.C., Donovan, D.M. 2015. Evolutionarily distinct bacteriophage endolysins featuring conserved peptidoglycan cleavage sites protect mice from MRSA infection. Antimicrobial Chemotherapy. 70(5):1453-1465.
Antibacterial and antibiofilm surfaces through Polydopamine-assisted immobilization of Lysostaphin as an antibacterial enzyme -
Yeroslavsky, G., Foster Frey, J.A., Donovan, D.M., Rahimipour, S. 2014. Antibacterial and antibiofilm surfaces through Polydopamine-assisted immobilization of Lysostaphin as an antibacterial enzyme. Langmuir. 31(3):1064–1073.
Lytic activity of the staphylolytic Twort phage endolysin CHAP domain is enhanced by the SH3b cell wall binding domain -
Becker, S.C., Korobova, O., Schischkova, N., Kopylov, P., Donovan, D.M., Abaev, I. 2014. Lytic activity of the staphylolytic Twort phage endolysin CHAP domain is enhanced by the SH3b cell wall binding domain. FEMS Microbiology Letters. 362(1):1-8.
Phage lytic proteins: biotechnological applications beyond clinical antimicrobials -
Rodriguez-Rubio,, L., Gutierrez, D., Donovan, D.M., Martinez, B., Rodriguez, A., Garcia, P. 2015. Phage lytic proteins: biotechnological applications beyond clinical antimicrobials. Chemical Rubber Company Critical Reviews in Biotechnology. DOI:10.3109/07388551.2014.993587.
Alternatives to Antibiotics: Recent Scientific Development -
Alternatives to antibiotics: recent scientific development -
Alternatives to Antibiotics: Recent Scientific Development -
Alternatives to Antibiotics: Recent Scientific Development -
Saccharomyces cerevisiae expressing bacteriophage endolysins reduce Lactobacillus contamination during fermentation Reprint Icon -
Khatibi, P.A., Roach, D.R., Donovan, D.M., Hughes, S.R., Bischoff, K.M. 2014. Saccharomyces cerevisiae expressing bacteriophage endolysins reduce Lactobacillus contamination during fermentation. Biotechnology for Biofuels. 7:104. DOI: 10.1186/1754-6834-7-104.
Can endolysin expressing yeast protect ethanolic fermentations and replace antibiotics in animal feed? -
Identification and characterization of peptidoglycan hydrolase constructs with activity in cow milk as potential antimicrobials for treatment of staphylococcal bovine mastitis -
Convergent evolution in peptidoglycan cut sites of phage endolysins protecting mice from methicillin-resistant Staphylococcus aureus septicemia -
Triple-acting antimicrobial treatment for drug-resistant and intracellular Staphylococcus aureus. -
Application of bacteriophage endolysins to reduce Lactobacillus contamination during fuel ethanol fermentation -
Khatibi, P.A., Roach, D.R., Donovan, D.M., Hughes, S.R., Bischoff, K.M. 2014. Application of bacteriophage endolysins to reduce Lactobacillus contamination during fuel ethanol fermentation [abstract]. American Society for Microbiology. Poster 2791. p. 218.
Generation of induced pluripotent stem cells from the pig -
Intravitreal injection of a chimeric phage endolysin Ply187 protects mice from Staphylococcus aureus endophthalmitis -
Singh, P.K., Donovan, D.M., Kumar, A. 2014. Intravitreal injection of a chimeric phage endolysin Ply187 protects mice from Staphylococcus aureus endophthalmitis. Antimicrobial Agents and Chemotherapy. 58(8):4621-4629.
Triple-acting antimicrobial treatment for drug-resistant and intracellular Staphylococcus aureus -
Alternatives to antibiotics: overview and engineered peptidoglycan hydrolases that reduce resistance development -
Sperm-mediated transgenesis in chicken using a PiggyBac transposon system -
Quansah, E., Long, J.A., Donovan, D.M., Becker, S.C., Telugu, B., Foster Frey, J.A., Urwin, N. 2014. Sperm-mediated transgenesis in chicken using a PiggyBac transposon system. BARC Poster Day. BARC Poster Day.
Phage lytic enzymes targeting streptococci -
Triple-acting antimicrobial treatment for drug-resistant and intracellular Staphylococcus aureus -
Sperm-mediated transgenesis in chicken using a PiggyBac transposon system -
Quansah, E., Long, J.A., Donovan, D.M., Becker, S.C., Telugu, B., Foster Frey, J.A., Urwin, N. 2014. Sperm-mediated transgenesis in chicken using a PiggyBac transposon system. Poultry Science Association Meeting Abstract. BARC Poster Day.
Triple-acting Peptidoglycan hydrolase treatment for drug-resistant and intracellular Staphylococcus aureus -
An investigation of the structure and function of antistaphylococcal endolysins using kinetic methods -
Filatova, L., Donovan, D.M., Becker, S.C., Kabanov, A., Klyachko, N.L. 2014. An investigation of the structure and function of antistaphylococcal endolysins using kinetic methods. Moscow University Chemistry Bulletin. 69(3):107-111.
Potential of the virion-associated peptidoglycan hydrolase HydH5 and its derivative fusion proteins in milk biopreservation -
Rodriguez-Rubio, L., Martinez, B., Donovan, D.M., Garcia, P., Rodriguez, A. 2013. Potential of the virion-associated peptidoglycan hydrolase HydH5 and its derivative fusion proteins in milk biopreservation. Applied Microbiology and Biotechnology. 8(1):e54828.
Bacteriophage endolysins expressed in yeast kill strains of Lactobacillus that contaminate fermentations -
Khatibi, P.A., Roach, D.R., Bischoff, K.M., Donovan, D.M., Hughes, S.R. 2013. Bacteriophage endolysins expressed in yeast kill strains of Lactobacillus that contaminate fermentations [abstract]. Society for Industrial Microbiology and Biotechnology. Poster P73.
Bacteriophage endolysin production in Nicotiana benthamiana plants -
Expression of bacteriophage endolysins in Saccharomyces cerevisiae -
Khatibi, P.A., Roach, D.R., Hughes, S.R., Donovan, D.M., Bischoff, K.M. 2013. Expression of bacteriophage endolysins in Saccharomyces cerevisiae [abstract]. American Society for Microbiology. Poster 293. p. 112.
Alternatives to antibiotics: a symposium on the challenges and solutions for animal production -
Seal, B.S., Gay, C.G., Lillehoj, H.S., Donovan, D.M. 2013. Alternatives to antibiotics: a symposium on the challenges and solutions for animal production. Animal Health Research Reviews. 14(1):78-87.
Physicochemical characterization of Staphylococcus aureus-lysing LysK enzyme in complexes with polycationic -
Filatova, L.Y., Donovan, D.M., Becker, S.C., Lebedev, D.N., Priyma, A.D., Koudriachova, E.V., Kabanov, A.V., Klyachko, N.L. 2013. Physicochemical characterization of Staphylococcus aureus-lysing LysK enzyme in complexes with polycationic. Biochimie. 95(9):1689-1996.
Undetectable bacterial resistance to phage lytic proteins from the Staphylococcus aureus bacteriophage vB_SauS-phiIPLA88 -
Rodriguez-Rubio, L., Martinez, B., Rodriguez, A., Donovan, D.M., Götz, F., García, P. 2013. Undetectable bacterial resistance to phage lytic proteins from the Staphylococcus aureus bacteriophage vB_SauS-phiIPLA88. PLoS One. 8(5):e64671.
Chimeric Ply187 endolysin kills Staphylococcus aureus more effectively than the parental enzyme. -
Mao, J., Donovan, D.M. 2013. Chimeric Ply187 endolysin kills Staphylococcus aureus more effectively than the parental enzyme. Applied and Environmental Microbiology. 342(1):30-36.
Bacteriophage-encoded lytic enzymes control growth of contaminating Lactobacillus found in fuel ethanol fermentations -
Roach, D.R., Khatibi, P.A., Bischoff, K.M., Hughes, S.R., Donovan, D.M. 2013. Bacteriophage-encoded lytic enzymes control growth of contaminating Lactobacillus found in fuel ethanol fermentations. Biotechnology for Biofuels. 6(1):20.
Meeting report of the european milecular Biology organization (EMBO) Symposium "Viruses of Meicrobes II" Brussels, July 2012 -
Ceyssens, P., Aertsen, A., Donovan, D.M., Lavigne, R., Sullivan, M., Debarbieux, L., Vaneechoutte, M. 2012. Meeting report of the european milecular Biology organization (EMBO) Symposium "Viruses of Meicrobes II" Brussels, July 2012. Research in Microbiology. 164:799-806.
Staphylococcus haemolyticus prophage endolysin relies on CHAP endopeptidase lytic activity for lysis from without -
Schmelcher, M., Korobova, O., Schischkova, N., Kiseleva, N., Kopylov, P., Pryamchuk, S., Donovan, D.M., Abaev, I. 2012. Staphylococcus haemolyticus prophage endolysin relies on CHAP endopeptidase lytic activity for lysis from without. FEMS Microbiology Letters. 162(2-3):289-298.
Bacteriophage endolysins as novel antimicrobials -
Schmelcher, M., Donovan, D.M., Loessner, M. 2012. Bacteriophage endolysins as novel antimicrobials. Future Microbiology.
Bacteriophage virion-associated peptidoglycan hydrolases: potential new enzybiotics -
Rodriguez-Rubio, L., Martinez, B., Donovan, D.M., Rodriguez, A., Garcia, P. 2013. Bacteriophage virion-associated peptidoglycan hydrolases: potential new enzybiotics. Critical Reviews in Microbiology. 39:427-434.
Staphylococcal phage 2638a endolysin is lytic for Staphylococcus aureus and harbors an inter-lytic-domain cryptic translational start site. -
Abaev, I., Foster Frey, J.A., Korobova, O., Shishkova, N., Kopylov, P., Pyramchuk, S., Schmelcher, M., Becker, S.C., Donovan, D.M. 2013. Staphylococcal phage 2638a endolysin is lytic for Staphylococcus aureus and harbors an inter-lytic-domain cryptic translational start site. Applied and Environmental Microbiology. 97(8):3449-3456.
Therapeutic use of chimeric bacteriophage (phage) lysins in staphylococcal endophthalmitis -
Chimeric phage lysins act synergistically with lysostaphin to kill mastitis causing staphylococcus aureus in murine mammary glands -
Schmelcher, M., Powell, A.M., Becker, S.C., Camp, M.J., Donovan, D.M. 2012. Chimeric phage lysins act synergistically with lysostaphin to kill mastitis causing staphylococcus aureus in murine mammary glands. Applied and Environmental Microbiology. 78(7):2297-305.
Enhanced staphylolytic activity of the Staphylococcus aureus bacteriophage vB_SauS-phiIPLA88 HydH5 virion associated peptidoglycan hydrolase: fusions, deletions and synergy with LysH5 -
Rodriguez, L., Martinez, B., Rodriguez, A., Donovan, D.M., Garcia, P. 2012. Enhanced staphylolytic activity of the Staphylococcus aureus bacteriophage vB_SauS-phiIPLA88 HydH5 virion associated peptidoglycan hydrolase: fusions, deletions and synergy with LysH5. Antimicrobial Agents and Chemotherapy. 78(7):2241-8.
Endolysins as antimicrobials -
Nelson, D.C., Schmelcher, M., Rodirguez, L., Klumpp, J., Pritchard, D.G., Dong, S., Donovan, D.M. 2012. Endolysins as antimicrobials. Advances in Virus Research.
Phage-based enzybiotics -
Shen, Y., Mitchell, M.S., Donovan, D.M., Nelson, D.C. 2012. Phage-based enzybiotics. Advances in Cellular and Molecular Microbiology: Bacteriophages in Health and Disease.
Lytic activity of the virion-associated peptidoglycan hydrolase HydH5 of staphylococcus aureus bacteriophage vB_SauS-phiIPLA88 -
Rodriguez, L., Martinez, B., Zhou, Y., Rodriguez, A., Donovan, D.M., Garcia, P. 2011. Lytic activity of the virion-associated peptidoglycan hydrolase HydH5 of staphylococcus aureus bacteriophage vB_SauS-phiIPLA88. BioMed Central Biology. 11:138-145.
Lysostaphin: molecular changes that preserve staphylolytic activity. -
Dopamine transporter-dependent and -independent striatal binding of the benztropine analog JHW 007, a cocaine antagonist with low abuse liability -
Kopajtic, T.A., Liu, Y., Surratt, C.K., Donovan, D.M., Newman, A.H., Katz, J.L. 2010. Dopamine transporter-dependent and -independent striatal binding of the benztropine analog JHW 007, a cocaine antagonist with low abuse liability. Journal of Pharmacology and Experimental Therapeutics. 335(3):703-714.
Staphylococcus aureus -
Weese, S.J., Mao, J., Donovan, D.M. 2010. Staphylococcus aureus. In: Fratamico, P., Liu, Y., Kathariou, S., editors. Genomes of Foodborne and Waterborne Pathogens. 1st edition. Washington, DC: American Society for Microbiology. p. 113-123.
LysK, the enzyme lysing Staphylococcus aureus cells: specific kinetic features and approaches towards stabilization -
Filatova, L.Y., Becker, S.C., Donovan, D.M., Gladilin, A.K., Klyachko, N.L. 2010. LysK, the enzyme lysing Staphylococcus aureus cells: specific kinetic features and approaches towards stabilization. Biochimie. 92(5):507-13.
Peptidoglycan Hydrolases for Control of Mastitis Pathogens -
Schmelcher, M., Becker, S.C., Foster Frey, J.A., Donovan, D.M. 2009. Peptidoglycan Hydrolases for Control of Mastitis Pathogens. Evergreen International Phage Meeting.
Engineering Antimicrobials Refractory to Resistance -
Becker, S.C., Pohl, C.S., Mohammadi, H., Schmelcher, M., Foster Frey, J.A., Lease, R.A., Don, S., Baker, J.R., Pritchard, D.G., Donovan, D.M. 2009. Engineering Antimicrobials Refractory to Resistance. Evergreen International Phage Meeting.
Peptidoglycan hydrolase fusion to protein transduction domains kill intracellular staphylococci. -
Becker, S.C., Foster Frey, J.A., Willard, R.R., Lease, R.A., Almeida, R., Marriott, I., Dong, S., Baker, J.R., Pritchard, D.G., Donovan, D.M. 2009. Peptidoglycan hydrolase fusion to protein transduction domains kill intracellular staphylococci. Evergreen International Phage Meeting.
Differentially conserved staphylococcal SH3b_5 cell wall binding domains confer increased staphylolytic and streptolytic activity to a streptococcal prophage endolysin domain. -
Becker, S.C., Foster-Frey, J., Stodola, A.J., Anacker, D., Donovan, D.M., 2009. Differentially conserved staphylococcal SH3b_5 cell wall binding domains confer identical staphylolytic activity to a streptococcal prophage endolysin lytic domain. FEMS Immunology and Medical Microbiology. 443(1-2):32-41.
Antimicrobials for staphylococcal pathogens that are refractory to resistance development -
Becker, S.C., Foster Frey, J.A., Willard, R.R., Lease, R.A., Almeida, R., Marriott, I., Dong, S., Pritchard, D.G., Donovan, D.M. 2009. Antimicrobials for staphylococcal pathogens that are refractory to resistance development. International Symposium on Antimicrobial Peptides.
ENGINEERING OF PEPTIDOGLYCAN HYDROLASES FOR CONTROL OF PATHOGENIC BACTERIA -
Schmelcher, M., Loessner, M.J., Donovan, D.M. 2009. ENGINEERING OF PEPTIDOGLYCAN HYDROLASES FOR CONTROL OF PATHOGENIC BACTERIA. BARC Poster Day.
Antimicrobials for mastitis causing pathogens that are refractory to resistance development -
Becker, S.C., Don, S., Baker, J.R., Foster Frey, J.A., Pritchard, D.G., Donovan, D.M. 2009. Antimicrobials for mastitis causing pathogens that are refractory to resistance development. BARC Poster Day.
Comparison of two bacteriophage derived enzymes with lytic activity against strains of Clostridium perfringens -
Simmons, I.M., Siragusa, G.R., Donovan, D.M., Seal, B.S. 2009. Comparison of two bacteriophage derived enzymes with lytic activity against strains of Clostridium perfringens. American Society for Microbiology.
Expression of Two Bacteriophage Enzymes that Lyse Clostridium perfringens which Share Sequences in the Cell Binding Domain of the Molecules but are Dissimilar in their Catalytic Enzymatic Domain -
Simmons, I.M., Siragusa, G., Donovan, D.M., Seal, B.S. 2009. Expression of Two Bacteriophage Enzymes that Lyse Clostridium perfringens which Share Sequences in the Cell Binding Domain of the Molecules but are Dissimilar in their Catalytic Enzymatic Domain. International Symposium on Antimicrobial Peptides.
Peptidoglycan hydrolase enzyme fusions are uniquely suited for treating multi-drug resistant pathogens -
Donovan, D.M., Becker, S.C., Dong, S., Baker, J.R., Foster Frey, J.A., Pritchard, D.G. 2009. Peptidoglycan hydrolase enzyme fusions are uniquely suited for treating multi-drug resistant pathogens. Biotech International 21(2):6-10.
LysK CHAP endopeptidase domain is required for lysis of live staphylococcal cells. -
Becker, S.C., Don, S., Baker, J.R., Foster Frey, J.A., Pritchard, D.G., Donovan, D.M. 2009. LysK CHAP endopeptidase domain is required for lysis of live staphylococcal cells. FEMS Microbiology Letter. 294(1):52-60.
Significant modulation of mitochondrial electron transport system by nicotine in various rat brain regions -
Wang, J., Kim, J., Donovan, D.M., Becker, K.G., Li, M.D. 2009. Significant modulation of mitochondrial electron transport system by nicotine in various rat brain regions. Mitochondrion. 9:186-195.
Genome walk of an unknown upstream region of myostatin gene in Spanish goats -
Singh, M., Amoah, E., Kannan, G., Stice, S., Donovan, D.M. 2009. Genome walk of an unknown upstream region of myostatin gene in Spanish goats. Reproductive Biology.
A Novel Use of a Bacteriophage Lysin, PlyC, as a Disinfectant against Streptococcus equi -
Hoopes, J.T., Stark, C.J., Kim, H.A., Sussman, D.J., Donovan, D.M., Nelson, D.C. 2009. A Novel Use of a Bacteriophage Lysin, PlyC, as a Disinfectant against Streptococcus equi. Applied and Environmental Microbiology. 75(5):1388-94.
IDENTIFICATION OF KNOWN PHAGE ENDOLYSIN GENES BY PCR -
The phage K lytic enzyme LysK and Lysostaphin are synergistic against MRSA. -
Becker, S., Donovan, D.M., Foster-Frye, J. 2008. The phage K lytic enzyme LysK and Lysostaphin act synergistically to kill MRSA. FEMS Microbiology Letter. 287(2):185-91.
SH3b Cell wall binding domains can enhance anti-staphylococcal activity of endolysin lytic domains. -
Engineering Antimicrobials that are Refractory to Resistance Development. -
Donovan, D.M., Becker, S.C. 2009. Engineering Antimicrobials that are Refractory to Resistance Development. Meeting Abstract.
LambdaSa2 prophage endolysin requires Cpl-7 binding domains and Amidase-5 domain for antimicrobial lysis of streptococci. -
Donovan, D.M., Foster-Frye,J., 2008. LambdaSa2 prophage endolysin requires Cpl-7 binding domains and Amidase-5 domain for antimicrobial lysis of streptococci. FEMS Microbiology Letter. 287(1):22-23.
Engineering MRSA antimicrobials that are refractory to resistance development -
Becker, S.C., Donovan, D.M., Foster Frey, J.A. 2008. Engineering MRSA antimicrobials that are refractory to resistance development. BARC Poster Day.
Use of a bacteriophage lysin for barnyard decontamination of Streptococcus equi -
Hoopes, J.T., Donovan, D.M., Sussman, D.J., Trayer, T.P., Stark, C.J., Nelson, D.C. 2008. Use of a bacteriophage lysin for barnyard decontamination of Streptococcus equi. American Society for Microbiology.
Genotyping Staphylococcus aureus allows one to identify bacteriophages harboring unknow endolysins. -
Donovan, D.M., Abaev, I., Schischkova, N., Myakinina, V., Korobova, O., Pecherskich, E., Kopylov, P., Kiseleva, N. 2008. Genotyping Staphylococcus aureus allows one to identify bacteriophages harboring unknow endolysins. Meeting Abstract.
Chronic nicotine exposure regulates the mitochondrial electron transport system in multiple brain regions of rat -
Wang, J., Kim, J., Donovan, D.M., Becker, K.G., Li, M. 2007. Chronic nicotine exposure regulates the mitochondrial electron transport system in multiple brain regions of rat. Society for Neuroscience Abstracts and Proceedings.
Resolving the database sequence discrepancies for the Staphylococcus aureus bacteriophage phi 11 amidase. -
Donovan D.M., Foster-Frey J., Garrett W.M., Blomberg, L. 2008. Resolving the database sequence discrepancies for the Staphylococcus aureus bacteriophage phi11 amidase. Journal of Basic Microbiology. 48(1):48-52.
Assessment of the in vitro binding of JHW 007, a dopamine transport inhibitor that blocks the effects of cocaine -
Kopajtic, T.A., Donovan, D.M., Newman, A.H., Katz, J.L. 2007. Assessment of the in vitro binding of JHW 007, a dopamine transport inhibitor that blocks the effects of cocaine. Society for Neuroscience Abstracts and Proceedings.
A summary of staphylococcal C-terminal SH3b_5 cell wall binding domains. -
Donovan, D.M., Stodola, A.J., Annacker, D. 2007. A summary of staphylococcal C-terminal SH3b_5 cell wall binding domains.. Evergreen International Phage Meeting.
Bacteriophage and peptidoglycan degrading enzymes with antimicrobial applications. -
Donovan, D.M. 2007. Bacteriophage and peptidoglycan degrading enzymes with antimicrobial applications. Review Article. Recent Patents on Biotechnology. 1(2):113-122.
Designer Antimicrobials: Peptidoglycan Hydrolase Module Shuffling -
BACTERIOPHAGE PHI11 ENDOLYSIN HAS ANTIMICROBIAL ACTIVITY AGAINST THE MASTITIS PATHOGENS STAPHYLOCOCCUS AUREUS AND COAGULASE NEGATIVE STAPHYLOCOCCUS -
Donovan, D.M., Foster Frey, J.A. 2006. Bacteriophage phi11 endolysin has antimicrobial activity against the mastitis pathogens staphylococcus aureus and coagulase negative staphylococcus. Applied and Environmental Microbiology. 265(1):133-139.
THE CELL LYSIS ACTIVITY OF THE STREPTOCOCCUS AGALACTIAE BACTERIOPHAGE B30 ENDOLYSIN RELIES ON THE CHAP ENDOPEPTIDASE DOMAIN. -
Donovan, D.M., Foster Frey, J.A., Dong, S., Rousseau, G., Moineau, S., Pritchard, D.G. 2006. The cell lysis activity of the streptococcus agalactiae bacteriophage b30 endolysin relies on the chap endopeptidase domain. Applied Environmental Microbiology. 72:5108-5112.
Designer Antimicrobials: Peptidoglycan Hydrolase Module Shuffling -
Donovan, D.M., Dong, S., Garrett, W.M., Foster Frey, J.A., Rousseau, G.M., Moineau, S., Pritchard, D.G. 2006. Designer Antimicrobials: Peptidoglycan Hydrolase Module Shuffling. American Society for Microbiology.
PETIDOGLYCAN HYDROLASE FUSIONS MAINTAIN PARENTAL SPECIFICITIES -
Donovan, D.M., Dong, S., Garrett, W.M., Rousseau, G.M., Moineau, S., Pritchard, D.G. 2006. Petidoglycan hydrolase fusions maintain parental specificities. Applied and Environmental Microbiology. 72:2988-2996.
ENGINEERING DISEASE RESISTANT CATTLE -
Donovan, D.M., Kerr, D.E., Wall, R.J. 2005. Engineering disease resistant cattle. Transgenic Research. 14(5):563-567.
FOLLICULAR EXPRESSION OF A HUMAN BETA-CELL LEUKEMIA/LYMPHOMA-2 (BCL-2)TRANSGENE DOES NOT DECREASE ATRESIA OR INCREASE OVULATION RATE IN SWINE -
Guthrie, H.D., Wall, R.J., Pursel, V.G., Foster Frey, J.A., Donovan, D.M., Dawson, H.D., Welch, G.R., Garrett, W.M. 2005. Follicular expression of a human B-cell leukemia/lymphoma-2 (Bcl-2)transgene does not decrease atresia or increase ovulation rate in swine. Journal Of Reproduction, Fertility And Development. 17(4):457-468.
FOLLICULAR EXPRESSION OF A HUMAN BETA-CELL LEUKEMIA/LYMPHOMA-2 (BCL-2)TRANSGENE DOES NOT DECREASE ATRESIA OR INCREASE OVULATION RATE IN SWINE -
Guthrie, H.D., Wall, R.J., Pursel, V.G., Donovan, D.M., Welch, G.R., Garrett, W.M. 2004. Follicular expression of a human beta-cell leukemia/lymphoma-2 (bcl-2)transgene does not decrease atresia or increase ovulation rate in swine [abstract]. Society for the Study of Reproduction Annual Meeting. p. 275.
EXPRESSION OF A GROUP B STREPTOCOCCUS BACTERIOPHAGE LYSIN IN MAMMALIAN CELLS. -
Donovan, D.M., Wall, R.J., Pritchard, D., Talbot, N.C. 2004. Expression of a group b streptococcus bacteriophage lysin in mammalian cells. [abstract]. American Society for Microbiology. No. M-035.
IDENTIFICATION AND CHARACTERIZATION OF METALLOTHIONEIN-1 AND 2 GENE EXPRESSION IN THE CONTEXT OF (±)3,4-METHYLENEDIOXYMETHAMPHETAMINE (MDMA)-INDUCED TOXICITY TO BRAIN DOPAMINERGIC NEURONS. -
Xie, T., Tong, L., Mccann, U., Yuan, J., Becker, K., Mechan, A., Cheadle, C., Donovan, D.M., Ricaurte, G. 2004. Identification and characterization of metallothionein-1 and 2 gene expression in the context of (±)3,4-methylenedioxymethamphetamine (mdma)-induced toxicity to brain dopaminergic neurons. Society for Neuroscience Abstracts and Proceedings. 24(32):7043-7050.
IDENTIFICATION AND CHARACTERIZATION OF METALLOTHIONEIN-1 AND 2 GENE EXPRESSION IN THE CONTEXT OF (±)3,4-METHYLENEDIOXYMETHAMPHETAMINE (MDMA)-INDUCED TOXICITY TO BRAIN DOPAMINERGIC NEURONS -
Xie, T., Tong, L., Mccann, U., Yuan, J., Becker, K., Mechan, A., Cheadle, C., Donovan, D.M., Ricaurte, A. 2004. Identification and characterization of metallothionein-1 and 2 gene expression in the context of (±)3,4-methylenedioxymethamphetamine (mdma)-induced toxicity to brain dopaminergic neurons. Journal of Neuroscience. 24(32):7043-7050.
IDENTIFICATION AND ANNOTATION OF 11,363 CLONES FROM A MURINE DOPAMINE NEURON-SPECIFIC CDNA LIBRARY USING SEQUENCING AND VARIOUS BIOINFORMATICS APPROACHES -
Li, M.D., Zhang, D., Taylor, W., Donovan, D.M., Becker, K.G. 2004. Identification and annotation of 11,363 clones from a murine dopamine neuron-specific cdna library using sequencing and various bioinformatics approaches. [abstract]. Society for Neuroscience Abstracts and Proceedings. 809.10.
FOLLICULAR EXPRESSION OF A HUMAN BETA-CELL LEUKEMIA/LYMPHOMA-2 (BCL-2)TRANSGENE DOES NOT DECREASE ATRESIA OR INCREASE OVULATION RATE IN SWINE. -
Guthrie, H.D., Wall, R.J., Pursel, V.G., Foster Frey, J.A., Donovan, D.M., Welch, G.R., Garrett, W.M. 2004. Follicular expression of a human beta-cell leukemia/lymphoma-2 (bcl-2)transgene does not decrease atresia or increase ovulation rate in swine. Society for the Study of Reproduction Annual Meeting. Abstract. p275.
MICROSOFT EXCEL SPREAD-SHEET BASED ANIMAL COLONY MANAGEMENT FOR GENETICALLY ALTERED MOUSE STRAINS. -
Donovan, D.M., Rowley, D.L., Williams, N.G. 2004. Microsoft excel spread-sheet based animal colony management for genetically altered mouse strains. Biotechniques. 37(1):50-56.
ROLE OF U-OPIOID RECEPTORS IN FORMALIN-INDUCED PAIN BEHAVIOR IN MICE -
Zhao, C., Tao, Y., Tall, J.M., Donovan, D.M., Meyer, R.A., Raja, S.N. Role of u-opioid receptors in formalin-induced pain behavior in mice. Journal of Experimental Neurology. 2003. 184(2):839-45.
MICROARRAY ANALYSIS REVEALS INTERLEUKIN-6 AS A NOVEL SECRETORY PRODUCT OF THE HYPOTHALAMO-NEUROHYPOPHYSEAL. -
Ghorbel, M.T., Sharman, G., Leroux, M., Barrett, T., Donovan, D.M., Becker, K.G., Murphy, D. Microarray analysis reveals interleukin-6 as a novel secretory product of the hypothalamo-neurohypophyseal.. Journal of Biological Chemistry.2003 MAY 23; 278(21):19280-5. EPUB 2003 MARCH 14
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