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United States Department of Agriculture

Agricultural Research Service

Related Topics

Christopher Silva (Chris)
Produce Safety and Microbiology Research
Chemistry

Phone: (510) 559-6135
Fax: (510) 559-6429

USDA, ARS, PWA, WRRC-PSM
800 BUCHANAN ST
ALBANY , CA 94710

Projects
Immunodiagnostics to Detect Prions and Other Important Animal Pathogens
In-House Appropriated (D)
  Accession Number: 424354
Molecular Identification and Characterization of Bacterial and Viral Pathogens Associated with Foods
In-House Appropriated (D)
  Accession Number: 430420
Identifying Host Proteins that Facilitate the Assembly of Viral and Phage Capsids and Small Molecules that Interfere with this Assembly
Trust Fund Cooperative Agreement (T)
  Accession Number: 426378

Publications (Clicking on the reprint icon Reprint Icon will take you to the publication reprint.)
Detecting and discriminating among Shiga toxins -
Silva, C.J., Erickson-Beltran, M.L., Skinner, C.B., Patfield, S.A., He, X., Wu, V.C. 2016. Detecting and discriminating among Shiga toxins. doi: 10.1021/acs.jafc.6b00379.
Using small molecule reagents to help distinguish among prion structural models -
Silva, C.J., Erickson-Beltran, M.L., Dynin, I.A. 2016. Using small molecule reagents to help distinguish among prion structural models. Prion 10(Supplement 1). p. S37-S38.
Identification of new molecular alterations in Fatal Familial Insomnia. -
Ferrer, I., Llorens, F., Frau-Mendez, M.A., Frnandez-Vega, I., Thune, K., Del Rio, J.A., Schmitz, M., Ansoleaga, B., Gotzmann, N., Cramm, M., Silva, C.J., Zerr, I., Zarranz, J. 2016. Identification of new molecular alterations in Fatal Familial Insomnia. Prion 10 (Supplement 1): S83.
Identification of new molecular alterations in Fatal Familial Insomnia -
Llorens, F., Thune, K., Schmitz, M., Cramm, M., Tahir, W., Gotzmann, N., Zerr, I., Silva, C.J., Frau-Mendez, M.A., Ansoleaga, B., Berjawi, S., Carmona, M., Ferrar, I., Fernandez, I., Zarranz, J. 2016. Identification of new molecular alterations in Fatal Familial Insomnia. Human Molecular Genetics. pii: ddw108.
Safe, rapid, and sensitive method of quantitating and distinguishing among Shiga toxins in complex media and human serum -
Wu, V.C., Silva, C.J., Erickson-Beltran, M.L., Skinner, C.B., Patfield, S.A., He, X. 2016. Safe, rapid, and sensitive method of quantitating and distinguishing among Shiga toxins in complex media and human serum. ARS Food Safety and Inspection Service Research Workshop. ARS-FSIS.
Covalent surface modification of prions: a mass spectrometry-based means of detecting distinctive structural features of prion strains Reprint Icon -
Silva, C.J., Erickson-Beltran, M.L., Dynin, I.C. 2016. Covalent surface modification of prions: a mass spectrometry-based means of detecting distinctive structural features of prion strains. Biochemistry. 55:894-902.
Mass spectrometry-based method of detecting and distinguishing type 1 and type 2 Shiga-like toxins in human serum Reprint Icon -
Silva, C.J., Erickson-Beltran, M.L., Skinner, C.B., Patfield, S.A., He, X. 2015. Mass spectrometry-based method of detecting and distinguishing type 1 and type 2 Shiga-like toxins in human serum. Toxins. 7:5236-5253.
The chemistry of prions: small molecules, protein conformers and mass spectrometry Reprint Icon -
Silva, C.J., Erickson-Beltran, M.L. 2015. The chemistry of prions: small molecules, protein conformers and mass spectrometry. Meeting Abstract. Prion (Supplement 1) 9: S70.
Using proteinase K to study the structure of prions. Reprint Icon -
Silva, C.J., Vazquez-Fernandez, E., Requena, J.R. 2015. Using proteinase K to study the structure of prions.. Meeting Abstract. Prion (Supplement 1) 9: S69-S70.
The view from above: The potential of aerial surveillance in identifying CWD infected herds. Reprint Icon -
Silva, C.J., Erickson-Beltran, M.L. 2015. The view from above: The potential of aerial surveillance in identifying CWD infected herds.. Meeting Abstract. Prion (Supplement 1) 9: S73..
PrP0\0 mice show behavioral abnormalities that suggest PrPC has a role in maintaining the cytoskeleton. Reprint Icon -
Schmitz, M., Silva, C.J. 2015. PrP0\0 mice show behavioral abnormalities that suggest PrPC has a role in maintaining the cytoskeleton.. Meeting Abstract. Prion (Supplement 1) 9: S73-S74..
Proteinase K and the structure of PrPse: the good, the bad, and the ugly Reprint Icon -
Silva, C.J., Vázquez-Fernández, E., Onikso, B., Requena, J.R. 2015. Proteinase K and the structure of PrPse: the good, the bad, and the ugly. Virus Research. doi: 10.1016/j.virusres.2015.03.008.
Detecting prions and discriminating among prion strains by discerning the differences in absence. -
Silva, C.J. 2015. Detecting prions and discriminating among prion strains by discerning the differences in absence.. Meeting Abstract. Volume 2015. Page: Lecture #23.
Behavioral abnormalities in prion protein knockout mice and the potential relevance of PrPc for the cytoskeleton Reprint Icon -
Schmitz, M., Zafar, S., Silva, C.J., Zerr, I. 2014. Behavioral abnormalities in prion protein knockout mice and the potential relevance of PrPc for the cytoskeleton. Prion. 8(6) 381-386.
Safe and effective means of detecting and quantitating Shiga-like toxins in attomole amounts Reprint Icon -
Silva, C.J., Erickson-Beltran, M.L., Skinner, C.B., Dynin, I., Hui, C., Patfield, S.A., Carter, J.M., He, X. 2014. Safe and effective means of detecting and quantitating Shiga-like toxins in attomole amounts. Analytical Chemistry. 86(10):4698-4706.
Overview of the Western Regional Research Center, Albany, California. Reprint Icon -
Silva, C.J. 2014. Overview of the Western Regional Research Center, Albany, California. Meeting Proceedings. #10.
Oxidized methionine is not a prion-specific covalent modification Reprint Icon -
Silva, C.J., Erickson-Beltran, M.L., Dynin, I., Hui, C., Carter, J.M. 2014. Oxidized methionine is not a prion-specific covalent modification. Meeting Abstract. Abstracts of the Papers of the American Chemical Society. 248: 230-BIOL.
Small molecules and antibodies: a means of distinguishing between PrPC and PrPSc Reprint Icon -
Silva, C.J., Erickson-Beltran, M.L., Dynin, I.A. 2014. Small molecules and antibodies: a means of distinguishing between PrPC and PrPSc [abstract]. Meeting Abstract. Current Topics in Biological Chemistry. Paper No. 12.
The physiological role of the normal cellular prion protein (PrPC). Reprint Icon -
Schmitz, M., Greis, C., Ottis, P., Silva, C.J., Schulz-Schaeffer, W.J., Wrede, A., Koppe, K., Requean, J.R. 2014. The physiological role of the normal cellular prion protein (PrPC) [abstract]. Meeting Abstract. Current Topics in Biological Chemistry. Paper No. 113.
Using mass spectrometry to detect and discriminate among Shiga-like toxins in the attomole range. Reprint Icon -
Silva, C.J., Erickson-Beltran, M.L., Skinner, C.B., Dynin, I.A., Hui, C., Patfield, S.A., He, X. 2014. Using mass spectrometry to detect and discriminate among Shiga-like toxins in the attomole range [abstract]. Meeting Abstract. Innovations in Mass Spectrometry. Paper No. 355.
Loss of prion protein leads to age-dependent behavioral abnormalities and changes in cytoskeletal protein expression -
Schmitz, M., Greis, C., Ottis, P., Silva, C.J., Schulz-Schaeffer, W., Wrede, A., Koppe, K., Onisko, B., Requena, J.R., Govindarajan, N., Korth, C., Fisher, A., Zerr, I. 2014. Loss of prion protein leads to age-dependent behavioral abnormalities and changes in cytoskeletal protein expression. Molecular Neurobiology. DOI: 10.1007/s12035-014-8655-3; 50(3):923-936.
Applying the tools of chemistry (mass spectrometry and covalent modification by small molecule reagents) to the detection of prions and the study of their structure Reprint Icon -
Silva, C.J. 2014. Applying the tools of chemistry (mass spectrometry and covalent modification by small molecule reagents) to the detection of prions and the study of their structure. Prion. 8(1):42-50. DOI: http://dx.doi.org/10.4161/pri.27891.
Distinguishing between PrPC and PrPSc using small molecule reagents -
Silva, C.J., Erickson-Beltran, M.L., Dynin, I., Carter, J.M. 2013. Distinguishing between PrPC and PrPSc using small molecule reagents. Meeting Abstract. Prion 2013 Banff, Alberta, Canada May 26-29, 2013. jdc..
Methods to differentiate protein conformers -
Onisko, B.C., Silva, C.J., Requena, J.R., Carter, J.M. 2013. Methods to differentiate protein conformers. Patent Application. US Patent 8,445,642.
Oxidation of methionine in PrP is dependent upon the oxidant and the amino acid two positions removed(Abstract) Reprint Icon -
Silva, C.J., Dynin, I.A., Erickson-Beltran, M.L., Hui, C., Carter, J.M. 2013. Oxidation of methionine in PrP is dependent upon the oxidant and the amino acid two positions removed(Abstract). Meeting Abstract. Prion 7:81. Available: https://landesbioscience.com/journals/prion/05-Prion7-2ARB-Posters%20PS.pdf.
A comparison of the structure of the PK-sensitive and PK-resistant forms of PrPSc(Abstract) -
Silva, C.J., Sajnani, G., Ramos, A., Pastrana, M.A., Onisko, B.C., Erickson-Beltran, M.L., Antaki, E.M., Dynin, I.A., Vazquez-Fernandez, E., Sigurdson, C.J., Carter, J.M., Requena, J.R. 2013. A comparison of the structure of the PK-sensitive and PK-resistant forms of PrPSc(Abstract). Meeting Abstract. PS 26.
Distinguishing between PrPC and PrPSc using small molecule reagents(Abstract) -
Silva, C.J., Erickson-Beltran, M.L., Dynin, I.A., Carter, J.M. 2013. Distinguishing between PrPC and PrPSc using small molecule reagents(Abstract). Meeting Abstract. PS 25.
Oxidation of methionine 216 in sheep and elk prion protein is highly dependent upon the amino acid at position 218 but is not important for prion propagation Reprint Icon -
Silva, C.J., Dynin, I.A., Erickson-Beltran, M.L., Requena, J.R., Balachandran, A., Onisko, B.C., Hui, C., Carter, J.M. 2013. Using mass spectrometry to detect prions and oxidized prions in scrapie-infected sheep and CWD-infected elk. Biochemistry. 52:2139-2147. doi:10.1021/bi3016795.
Structural organization of mammalian prions as probed by limited proteolysis -
Vasquez-Fernandez, E., Alonso, J., Pastrana, M.A., Ramos, A., Stitz, L., Vidal, E., Dynin, I.A., Petsch, B., Silva, C.J., Requena, J.R. 2012. Structural organization of mammalian prions as probed by limited proteolysis. PLoS One. 7(11):e50111. doi:10.1371/journal.pone.0050111.
Oxidation of methionine in PrP is dependent upon the oxidant and the amino acid two positions removed -
Detecting and discriminating among pathogenic protein conformers(prions), using mass spectrometry-based and antibody-based approaches(Abstract) -
Silva, C.J., Onisko, B.C., Dynin, I.A., Erickson-Beltran, M.L., Hui, C., Carter, J.M. 2012. Detecting and discriminating among pathogenic protein conformers(prions), using mass spectrometry-based and antibody-based approaches(Abstract). Meeting Abstract. Poster 216.
Using synthetic small molecule reagents and antibodies to distinguish among PrP conformers: new uses for old antibodies -
Silva, C.J., Erickson-Beltran, M.L., Dynin, I.A., Carter, J.M. 2012. Using synthetic small molecule reagents and antibodies to distinguish among PrP conformers: new uses for old antibodies. Meeting Abstract. Prion 6(supplement),90.
Probing the structure of GPI-less PrPSc by limited proteolysis(Abstract) -
Vazquez-Fernandez, E., Pastrana, M., Ramos, A., Alonso, J., Stitz, L., Vindal, E., Dynin, I.A., Silva, C.J. 2012. Probing the structure of GPI-less PrPSc by limited proteolysis(Abstract). Meeting Abstract. Prion 6(supplement),26-27.
Molecular approaches to detecting and discriminating among prions, a class of pathogenic molecules(Abstract) -
Silva, C.J., Onisko, B.C., Dynin, I.A., Erickson-Beltran, M.L., Hui, C., Carter, J.M. 2012. Molecular approaches to detecting and discriminating among prions, a class of pathogenic molecules(Abstract). Meeting Abstract. Poster, 615.
Human and rat brain lipofuscin proteome -
Otts, P., Koppe, K., Onisko, B.C., Dynin, I.A., Arzberger, T., Kretzschmar, H., Requena, J.R., Silva, C.J., Huston, J.P., Korth, C. 2012. Human and rat brain lipofuscin proteome. Proteomics. 12(15-16):2445-2454. doi:10.1002/pmic.201100668.
PK-sensitive PrPSc is infectious and shares basic structural features with PK-resistant PrPSc Reprint Icon -
Sajnani, G., Silva, C.J., Ramos, A., Pastrana, M.A., Onisko, B.C., Erickson-Beltran, M.L., Antaki, E.M., Sigurdson, C.J., Carter, J.M., Requena, J.R. 2012. PK-sensitive PrPSc is infectious and shares basic structural features with PK-resistant PrPSc. PLoS Pathogens. 8(3):e1002547. doi:10.1371/journal.ppat.1002547.
Using small molecule reagents to selectively modify epitopes based on their conformation -
Silva, C.J. 2012. Using small molecule reagents to selectively modify epitopes based on their conformation. Prion. 6:(2)165-175.
Using Mass Spectrometry to Diagnose Prion diseases: Can we do that? -
Diagnosing Prion Diseases: Mass Spectrometry-Based Approaches -
Silva, C.J., Erickson, M.L., Dynin, I.A., Onisko, B.C., Carter, J.M. 2011. Diagnosing Prion Diseases: Mass Spectrometry-Based Approaches. Meeting Abstract. Prion, Volume 5 (Supplement), April/May/June 2011. page 85.
Probing structural differences between PrPC and PrPSc by surface nitration and acetylation: evidence of conformational change in the C-terminus -
Gong, B., Ramos, A., Vazquez-Fernandez, E., Silva, C.J., Alonso, J., Requena, J.R. 2011. Probing structural differences between PrPC and PrPSc by surface nitration and acetylation: evidence of conformational change in the C-terminus. Biochemistry. 50:4963-4972.
Utility of mass spectrometry in the diagnosis of prion diseases -
Silva, C.J., Onisko, B.C., Dynin, I.A., Erickson-Beltran, M.L., Requena, J.J., Carter, J.M. 2011. Utility of mass spectrometry in the diagnosis of prion diseases. Analytical Chemistry. 83(5):1609-1615. doi:10.1021/ac102527w.
Mass spectrometry and prions: The need to simplify and remove oil from the system -
Silva, C.J. 2010. Mass spectrometry and prions: The need to simplify and remove oil from the system. International News on Fats, Oils and Related Materials.21(8):517-520.
Detecting and quantifying prions: Mass spectrometry-based approaches -
Assessing the Role of Oxidized Methionine at Position 213 in the Formation of Prions in Hamsters -
Silva, C.J., Onisko, B.C., Dynin, I.A., Erickson, M.L., Vensel, W.H., Requena, J., Antaki, E.M., Carter, J.M. 2010. Assessing the Role of Oxidized Methionine at Position 213 in the Formation of Prions in Hamsters. Journal of Biochemistry. (49):1854-1861.
Mass Spectrometry of Prions: Approaches to Conformational Distinction -
Silva, C.J., Onisko, B., Dynin, I.A., Erickson, M.L., Carter, J.M. 2009. Mass Spectrometry of Prions: Approaches to Conformational Distinction. [Abstract]. AOAC 123rd Annual Meeting & Exposition. S-902. p68-69
Disinfectants and Prions -
Silva, C.J. 2009. Disinfectants and Prions. [Abstract]. AOAC Annual Meeting & Exposition S-401. p63
Induction of Purple Sulfur Bacterial Growth in Dairy Wastewater Lagoons by Circulation -
Mcgarvey, J.A., Miller, W.G., Lathrop, J.R., Silva, C.J., Bullard, G. 2009. Induction of Purple Sulfur Bacterial Growth in Dairy Wastewater Lagoons by Circulation. Letters in Applied Microbiology. 49:427-433.
Prion Infectivity Assays. -
Mass Spectrometric Approaches to Detecting and Quantifying Prions -
Silva, C.J., Onisko, B.C., Dynin, I.A., Erickson, M.L. 2009. Mass Spectrometric Approaches to Detecting and Quantifying Prions. [Abstract]. PrP Canada p28.
Prion diseases: A little prevention can prevent a catastrophe -
Silva, C.J. 2009. Prion diseases: A little prevention can prevent a catastrophe. [Abstract]. AOAC AM-1 p1.
Prions: The Chemistry of Infectious Proteins -
Silva, C.J. 2008. Prions: The Chemistry of Infectious Proteins [Abstract]. American Oil Chemists' Society. S-401. p.63.
Mass spectrometric approaches to detecting prions and protein conformers -
Silva, C.J., Onisko, B.C., Dynin, I.A., Erickson, M.L. 2009. Mass spectrometric approaches to detecting prions and protein conformers. [Abstract] AOAC S-401. p63
Prion Diseases -
Silva, C.J. 2009.Prion Diseases:Sequelae and Long Term Consequences of Infectious Diseases. District of Columbia:ASM Press. 442p.
Mass Spectrometric Approaches to Detecting and Quantifying Prions -
Silva, C.J., Onisko, B.C., Dynin, I.A., Erickson, M.L., Carter, J.M. 2008. Mass Spectrometric Approaches to Detecting and Quantifying Prions. [Abstract]. Third International CWD Symposium. Advancing the Science and Developing the Tools. Vol 3. p23.
Bovine Spongiform Encephalopathy -
Harman, J.L., Silva, C.J. 2009. Bovine Spongiform Encephalopathy. Journal of the American Veterinary Medical Association. (2009)234(1):59-72
Sensitive, Preclinical Detection of Prions in Brain by nanospray liquid chromatography/tandem mass spectrometry -
Onisko, B.C., Silva, C.J., Dynin, I.A., Erickson, M.L., Vensel, W.H., Hnasko, R.M., Requena, J.R., Carter, J.M. 2007. Sensitive, Preclinical Detection of Prions in Brain by nanospray liquid chromatography/tandem mass spectrometry. Rapid Communications in Mass Spectrometry. 21(24):4023-4026.
Pathobiology and diagnosis of animal transmissible spongiform encephalopathies: current knowledge, research gaps, and opportunities -
Kehrli, Jr., M.E., O'Rourke, K.I., Hamir, A.N., Richt, J.A., Nicholson, E.M., Silva, C.J., Edelman, D., Gay, C.G. 2007. Pathobiology and diagnosis of animal transmissible spongiform encephalopathies: current knowledge, research gaps, and opportunities [government white paper]. Beltsville, MD: Interagency Working Group on Prion Science, Subcommittee on Pathobiology and Diagnostics. USDA, Agriculture Research Service. 33 p.
Mass Spectrometric Detection of Attomole Amounts of the Prion Protein by nanoLC-MS-MS -
Onisko, B.C., Dynin, I.A., Requena, J.R., Silva, C.J., Erickson, M.L., Carter, J.M. 2007. Mass Spectrometric Detection of Attomole Amounts of the Prion Protein by nanoLC-MS-MS. Journal of American Society for Mass Spectrometry. Volume(18); 1070-1079
State of BSE in the United States Reprint Icon -
Silva, C.J. 2007. State of BSE in the United States. The National Provisioner. April 2007: Page 15. http://www.provisioneronline.com/content.php?s=NP/2007/04&p=15
MASS SPECTROMETRIC DETECTION OF ATTOMOLE AMOUNTS OF THE PRION PROTEIN, PRP 27-30, BY NANOLC-MS-MS -
Onisko, B.C., Requena, J., Silva, C.J., Dynin, I.A., Carter, J.M. 2006. Mass spectrometric detection of attomole amounts of the prion protein, prp 27-30, by nanolc-ms-ms. [Abstract]. 232nd ACS National Meeting and Exposition. Poster AGFD 200
PRIONS: PATHOLOGICAL PROTEINS AT THE INTERFACE OF OIL AND WATER -
Silva, C.J. 2006. Prions: pathological proteins at the interface of oil and water [Abstract]. 97th AOCS Annual Meeting & Expo, April 30-May3, 2006, St. Louis, MO. Abstract # FM3-1; page 43. Available: http://www.aocs.org/archives/am2006/session.asp?session=FM+3%3A+Animal%2DHuman+Linked+Health+and+Nutrition+Issues
PRIONS: PATHOLOGICAL PROTEINS AT THE INTERFACE OF OIL AND WATER. -
Silva, C.J. 2006. Prions: pathological proteins at the interface of oil and water. Inform. 17(2), pp. 76-77+79.
HETEROLOGOUS PRODUCTION OF DAPTOMYCIN IN STREPTOMYCES LIVIDANS -
Penn, J., Li, X., Whiting, A., Latif, M., Silva, C.J., Brian, P., Davies, J., Miao, V., Wrigley, S.K., Baltz, R.H. 2005. Heterologous production of daptomycin in streptomyces lividans [Epub ahead of print]. Journal of Industrial Microbiology and Biotechnology. Nov 1:1-8.
BACTERIAL POPULATION STRUCTURE OF DAIRY WASTEWATERS -
Mcgarvey, J.A., Miller, W.G., Sanchez, S., Silva, C.J. 2005. Bacterial population structure of dairy wastewaters [abstract]. International Union of Microbiological Societies, July 23-28, 2005, San Francisco, CA. Poster No. B1102.
BACTERIAL AND CHEMICAL COMPOSITION OF DAIRY WASTEWATER -
Mcgarvey, J.A., Miller, W.G., Sanchez, S., Silva, C.J. 2005. Bacterial and chemical composition of dairy wastewater [abstract]. American Society for Microbiology General Meeting, 6/5-6/9/05, Atlanta, GA. P. 008.
COMPARISON OF BACTERIAL POPULATIONS AND CHEMICAL COMPOSITION OF DAIRY WASTEWATER HELD IN CIRCULATED AND STAGNANT LAGOONS -
Mcgarvey, J.A., Miller, W.G., Sanchez, S., Silva, C.J., Whitehand, L.C. 2005 Comparison of bacterial populations and chemical composition of dairy wastewater held in circulated and stagnant lagoons [abstract]. Journal of Applied Microbiology. 99(10):867-877.
DAPTOMYCIN BIOSYNTHESIS IN STREPTOMYCES ROSEOSPORUS: CLONING AND ANALYSIS OF THE GENE CLUSTER AND REVISION OF PEPTIDE STEREOCHEMISTRY -
Miao, V., Coeffet-LeGal, M-F, Brian, P., Brost, R., Penn, J., Whiting, A., Martin, S. Ford, R., Parr, I., Bouchard, M., Silva, C.J., Wrigley, S.K., Baltz, R.H. 2005. Daptomycin biosynthesis in Streptomyces roseosporus: cloning and analysis of the gene cluster and revision of peptide stereochemistry. Microbiology. 151(5):1507-1523.
SCRAPIE: THE VERY MODEL OF AN INFECTIOUS(PROTEIN)ISOFORM -
Silva, C.J. 2005. Scrapie: The Very Model of an Infectious (Protein) Isoform [abstract]. 96th AOCS Annual Meeting & Expo. Salt Lake City, UT, May 2, 2005.
PRIONS: THE TWISTED TALE OF INFECTIOUS PROTEIN ISOFORMS -
Silva, C.J. 2004. Prions: The Twisted Tale of Infectious Protein Isoforms. The Vortex. American Chemical Society, California Section. 65(6):4.
CHOLESTEROL: A MARKER FOR THE PRESENCE OF ANIMAL MATERIAL IN FEED -
Last Modified: 5/2/2016
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