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ARS Home » Midwest Area » West Lafayette, Indiana » Crop Production and Pest Control Research » People & Locations » Steven Scofield (Steve)
Steven Scofield (Steve)
Crop Production and Pest Control Research
Research Leader

Phone: (765) 494-3674
Fax: (765) 494-3452

CROP PRODUCTION AND PEST CONTROL RESEARCH UNIT
915 WEST STATE STREET
WEST LAFAYETTE, IN 479072054
Projects
Molecular Mechanisms of Host-Fungal Pathogen Interactions in Cereal Crops
In-House Appropriated (D)
  Accession Number: 432624
Purdue Biological Safety Committee Agreement
Non-Funded Cooperative Agreement (N)
  Accession Number: 433407
Improvement of Oat Germplasm for Resistance/Tolerance to Barley and Cereal Yellow Dwarf Virus Utilizing Marker-Assisted-Selection Breeding
Non-Assistance Cooperative Agreement (S)
  Accession Number: 433659
Identifying Genes for Resistance to Septoria tritici Blotch and Other Diseases in Wheat
Non-Assistance Cooperative Agreement (S)
  Accession Number: 435533
Ag-Discovery Summer Enrichment Program
Non-Assistance Cooperative Agreement (S)
  Accession Number: 436853
Developing Methods for More Efficient Wheat Transformation
Non-Assistance Cooperative Agreement (S)
  Accession Number: 437046
Engineering Gene-for-Gene Resistance to Fusarium Head Blight in Wheat and Barley (Rothamsted)
Non-Assistance Cooperative Agreement (S)
  Accession Number: 438746
Engineering Gene-for-Gene Resistance to Fusarium Head Blight in Wheat and Barley (Indiana)
Non-Assistance Cooperative Agreement (S)
  Accession Number: 438747
Tar Spot of Corn: Characterizing the Ecology and Epidemiology of an Understudied and High-Profile Disease
Non-Assistance Cooperative Agreement (S)
  Accession Number: 438887

Publications (Clicking on the reprint icon Reprint Icon will take you to the publication reprint.)
Mitochondrial Genome Sequence of Phytophthora sansomeana and Comparative Analysis of Phytophthora Mitochondrial Genomes Reprint Icon - (Peer Reviewed Journal)
Cai, G., Scofield, S.R. 2020. Mitochondrial Genome Sequence of Phytophthora sansomeana and Comparative Analysis of Phytophthora Mitochondrial Genomes. PLoS ONE. 15(5). https://doi.org/10.1371/journal.pone.0231296.
Phenotypic and molecular characterization of Hessian fly resistance in diploid wheat, Aegilops tauschii Reprint Icon - (Peer Reviewed Journal)
Nemacheck, J.A., Schemerhorn, B.J., Scofield, S.R., Subramanyam, S.N. 2019. Phenotypic and molecular characterization of Hessian fly resistance in diploid wheat, Aegilops tauschii. Biomed Central (BMC) Plant Biology. 19:439. https://doi.org/10.1186/s12870-019-2058-6.
Virus-induced gene silencing (VIGS) for functional characterization of disease resistance genes in barley seedlings Reprint Icon - (Peer Reviewed Journal)
Gunupuru, L.R., Perochon, A., Ali, S.S., Scofield, S.R., Doohan, F.M. 2018. Virus-induced gene silencing (VIGS) for functional characterization of disease resistance genes in barley seedlings. Methods in Molecular Biology. 1900:95-114. https://doi.org/10.1007/978-1-4939-8944-7_7.
Thymol-based sub-micron emulsions exhibit antifungal activity against Fusarium graminearum and inhibit Fusarium head blight (FHB) in wheat - (Peer Reviewed Journal)
Gill, T.A., Li, J., Doppler, M., Scofield, S.R. 2016. Thymol-based sub-micron emulsions exhibit antifungal activity against Fusarium graminearum and inhibit Fusarium head blight (FHB) in wheat. Journal of Applied Microbiology. 4:1103-1116. doi:10.1111/jam.13195.
TaFROG encodes a Pooideae orphan protein that interacts with SnRK1 and enhances resistance to the mycotoxigenic fungus fusarium graminearum. - (Peer Reviewed Journal)
Perochon, A., Jianguang, J., Kahla, A., Arunachalam, C., Scofield, S.R., Bowden, S., Wallington, E., Doohan, F. 2015. TaFROG, a novel plant orphan gene, interacts with SnRK1 and enhances resistance to a mycotoxigenic fungus. Plant Physiology. 169(4): 2895-2906. doi: http://dx.doi.org/10.1104/pp.15.01056.
Virus-induced gene silencing (VIGS) in barley seedling leaves - (Peer Reviewed Journal)
Gunupuru, L.R., Ali, S.S., Doohan, F.M., Scofield, S.R. 2015. Virus-induced gene silencing (VIGS) in barley seedling leaves. Bio-protocol. 5(12): 1506-8.
A wheat ABC transporter contributes to both grain formation and mycotoxin tolerance - (Peer Reviewed Journal)
Walter, S., Kahla, A., Arunachalam, C., Perochon, A., Khan, M.R., Scofield, S.R., Doohan, F.M. 2015. A wheat ABC transporter contributes to both grain formation and mycotoxin tolerance. Journal of Experimental Botany. 66(9):2583-2593. DOI:10.1093/jxb/erv048.
Plant disease resistance is augmented in uzu barley lines modified in the brassinosteroid receptor BRI1 - (Peer Reviewed Journal)
Ali, S.S., Gunupuru, L.R., Kumar, G.S., Khan, M., Scofield, S.R., Nicholson, P., Doohan, F.M. 2014. Plant disease resistance is augmented in uzu barley lines modified in the brassinosteroid receptor BRI1. Biomed Central (BMC) Plant Biology. 14:227.
New tools to determine gene function in maize ()
Benavente, L.M., Scofield, S.R. 2011. New tools to determine gene function in maize. New Phytologist. 189:363-365.
Intracellular expression of a host-selective toxin, ToxA, in diverse plants phenocopies silencing of a ToxA-interacting protein, ToxABP1 - (Peer Reviewed Journal)
Manning, V.A., Chu, A.L., Scofield, S.R., Ciufetti, L.M. 2010. Intracellular expression of a host-selective toxin, ToxA, in diverse plants phenocopies silencing of a ToxA-interacting protein, ToxABP1. New Phytologist. 187:1034-1047.
Virus-induced gene silencing of WRKY53 and an inducible phenylalanine ammonia-lyase in wheat reduces aphid resistance - (Peer Reviewed Journal)
Van Eck, L., Schultz, T., Leach, J.E., Scofield, S.R., Peairs, F.B., Botha, A., Lapitan, N.V. 2010. Virus-induced gene silencing of WRKY53 and an inducible phenylalanine ammonia-lyase in wheat reduces aphid resistance. Plant Biotechnology Journal. 9:1023-1032.
Methods for Virus-Induced Gene Silencing in Hexaploid Wheat using barley stripe mosaic virus vectors ()
Scofield, S.R., Brandt, A.S. 2012. Methods for Virus-Induced Gene Silencing in Hexaploid Wheat using barley stripe mosaic virus vectors. Methods in Molecular Biology. 894:93-112.
Rapid Determination of Gene Function by Virus-Induced Gene Silencing in Wheat and Barley - (Peer Reviewed Journal)
Scofield, S.R., Gillespie, M., Cakir, C. 2010. Rapid Determination of Gene Function by Virus-Induced Gene Silencing in Wheat and Barley. Crop Science. 50:77-84.
Rapid Determination of Gene Function by Virus-induced Gene Silencing in Wheat and Barley - (Peer Reviewed Journal)
Cakir, C., Scofield, S.R., Gillespie, M.E. 2010. Rapid determination of gene function by virus-induced gene silencing in wheat and barley. Crop Science. 50(2, Suppl. S):S77-S84.
Two Genes Encoding Structurally Different CC-NB-LRR Proteins are Required for Lr10-Mediated Leaf Rust Resistance in Wheat of Two Ploidy Levels - (Peer Reviewed Journal)
Loutre, C., Wicker, T., Travella, S., Galli, P., Scofield, S.R., Fahima, T., Feuillet, C., Keller, B. 2009. Two Genes Encoding Structurally Different CC-NB-LRR Proteins are Required for Lr10-Mediated Leaf Rust Resistance in Wheat of Two Ploidy Levels. Plant Journal. 60:1043-54.
Functional dissection of wheat disease resistance pathways - (Abstract Only)
Scofield, S.R. 2009. Functional dissection of wheat disease resistance pathways. Plant Genome Conference Proceedings.
Small-Interfering RNAs from Natural Antisense Transcripts Derived from a Cellulose Synthase Gene Modulate Cell Wall Biosynthesis in Barley - (Peer Reviewed Journal)
Held, M.A., Penning, B., Kessans, S.A., Yong, W., Scofield, S.R., Brandt, A.S., Carpita, N.C. 2008. Small-Interfering RNAs from Natural Antisense Transcripts Derived from a Cellulose Synthase Gene Modulate Cell Wall Biosynthesis in Barley. Proceedings of the National Academy of Sciences. 105:20534-10539.
Resources for Virus-Induced Gene Silencing (VIGS) in the Grasses - (Peer Reviewed Journal)
Scofield, S.R., Nelson, R. 2009. Resources for Virus-Induced Gene Silencing (VIGS) in the Grasses. Plant Physiology. 149:152-157.
Evaluating the ability of the barley stripe mosaic virus-induced gene silencing system to simultaneously silence two wheat genes - (Peer Reviewed Journal)
Cakir, C., Scofield, S.R. 2008. Evaluating the ability of the barley stripe mosaic virus-induced gene silencing system to simultaneously silence two wheat genes. Cereal Research Communications. 36:21-222.
Evaluating the Ability of the Barley Stripe Mosaic Virus-Induced Gene Silencing System to Simultaneously Silence Two Wheat Genes - (Peer Reviewed Journal)
Scofield, S.R., Cakir, C. 2008. Evaluating the Ability of the Barley Stripe Mosaic Virus-Induced Gene Silencing System to Simultaneously Silence Two Wheat Genes. Cereal Research Communications. 36:217-222.
Genetics of host-pathogen interactions in the wheat-Stagonospora nodorum pathosystem - (Proceedings)
Faris, J.D., Lu, H.J., Zhang, Z., Liu, Z.H., Xu, S.S., Chu, C.G., Abeysekara, N., Fellers, J.P., Cloutier, S., Keller, B., Scofield, S.R., Friesen, T.L. 2008. Genetics of host-pathogen interactions in the wheat-Stagonospora nodorum pathosystem. International Wheat Conference Proceedings. Australia Aug 24-29
A History of Grafting ()
Mudge, K., Goldschmidt, E., Scofield, S.R., Janick, J. 2009. A History of Grafting. Horticultural Reviews, Plant Breeding Reviews. 35:437-487.
A guardian of grasses: specific origin and conservation of a unique disease-resistance gene in the grass lineage - (Peer Reviewed Journal)
Sindhu, A., Chintamanani, S., Scofield, S.R., Brandt, A.S., Johal, G. 2008. A guardian of grasses: specific origin and conservation of a unique disease-resistance gene in the grass lineage. Journal of the National Academy of Sciences. 105:1762-1767.
Rapid Identification of Genes Contributing to FH Resistance in Wheat - (Abstract Only)
Scofield, S.R. 2007. Rapid Identification of Genes Contributing to FH Resistance in Wheat. The Canadian Workshop on Fusarium Head Blight. p. 76.
Wheat-rice colinearity and chromosome walking at the Snn1 locus in wheat - (Abstract Only)
Reddy, L., Friesen, T.L., Meinhardt, S.W., Chao, S., Scofield, S.R., Faris, J.D. 2007. Wheat-rice colinearity and chromosome walking at the Snn1 locus in wheat. Meeting Abstract. National Wheat Genomics Conference Abstracts. pg 28
Genetic analysis of host-toxin interactions in the wheat-Stagonospora nodorum pathosystem - (Abstract Only)
Faris, J.D., Friesen, T.L., Lu, H., Reddy, L., Zhang, Z., Liu, Z., Xu, S.S., Meinhardt, S.W., Fellers, J.P., Oliver, R.P., Solomon, P.S., Chao, S., Chu, C., Abeysekara, N., Scofield, S.R. 2007. Genetic analysis of host-toxin interactions in the wheat-Stagonospora nodorum pathosystem. Meeting Abstract. National Wheat Genomics Conference abstracts pg. 11
Virus-Induced Gene Silencing in Hexaploid Wheat - (Abstract Only)
Scofield, S.R., Cakir, C., Brandt, A.S., Kong, L., Anderson, J.M. 2007. Virus-Induced Gene Silencing in Hexaploid Wheat. Plant and Animal Genome VX Conference Abstracts. p. 794.
A VIRUS-INDUCED GENE SILENCING SYSTEM FOR THE ANALYSIS OF DISEASE RESISTANCE PATHWAYS IN WHEAT AND BARLEY - (Abstract Only)
DEVELOPMENT OF A VIRUS-INDUCED GENE-SILENCING SYSTEM FOR HEXAPLOID WHEAT AND ITS USE IN FUNCTIONAL ANALYSIS OF THE LR21-MEDIATED LEAF RUST RESISTANCE PATHWAY - (Peer Reviewed Journal)
Scofield, S.R., Huang, L., Brandt, A.S., Gill, B.S. 2005. Development of a virus-induced gene-silencing system for hexaploid wheat and its use in functional analysis of the lr21-mediated leaf rust resistance pathway. Plant Physiology. 10.1104:105.061861
FUNCTIONAL PATHOGENOMICS IN CEREALS - (Abstract Only)
Scofield, S.R., Brandt, A.S., Anderson, J.M., Crane, C.F., Goodwin, S.B., Ohm, H., Williams, C.E., Lohret, T., Crasta, O. 2004. Functional pathogenomics in cereals (abstract). Plant Animal and Microbe Genome XII. Abstract No. P839.
FUNCTIONAL PATHOGENOMICS IN CEREALS - (Abstract Only)
ANALYSIS OF THE WHEAT DEFENSE TRANSCRIPTOME - (Proceedings)
Scofield, S.R., Anderson, J.M., Crane, C.F., Goodwin, S.B., Ohm, H., Williams, C.E., Loret, T., Crasta, O. 2003. Analysis of the wheat defense transcriptome. Proceedings of the Tenth International Wheat Genetics Symposium, Paestum, Italy. 1:407-410.
QUANTITATIVE ANALYSIS OF WHEAT DEFENSE-GENE EXPRESSION IN RESPONSE TO INSECT, FUNGAL, AND VIRAL PESTS - (Proceedings)
Anderson, J.M., Williams, C.E., Goodwin, S.B., Scofield, S.R., Ohm, H.W. 2003. Quantitative analysis of wheat defense-gene expression in response to insect, fungal, and viral pests. In: Proceedings of the Tenth International Wheat Genetics Symposium, September 1-6, 2003. Paestum, Italy. 1:323-326.
TRANSCRIPTION PROFILING OF WHEAT DEFENSE RESPONSES: HESSIAN FLY VERSUS MICROBIAL PATHOGENS - (Abstract Only)
Williams, C.E., Goodwin, S.B., Ohm, H.W., Anderson, J.M., Crane, C.F., Scofield, S.R., Lohret, T.A., Crasta, O.R. 2003. Transcription profiling of wheat defense responses: hessian fly versus microbial pathogens. In: Proceedings of the Entomological Society of America Regional Meetings. Entomological Society of American-North Central Branch, March 23-26, 2003, Madison, Wisconsin. Available: http://esa.ent.iastate.edu/confreg/?gridaction=viewonepresentation&year=2003 &presnum=083
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