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ARS Home » Midwest Area » West Lafayette, Indiana » Crop Production and Pest Control Research » People & Locations » Steven Scofield (Steve)
Steven Scofield (Steve)
Crop Production and Pest Control Research
Research Leader

Phone: (765) 494-3674
Fax: (765) 494-3452

CROP PRODUCTION AND PEST CONTROL RESEARCH UNIT
915 WEST STATE STREET
WEST LAFAYETTE , IN 479072054
Projects
Molecular Mechanisms of Host-Fungal Pathogen Interactions in Cereal Crops
In-House Appropriated (D)
  Accession Number: 432624
Purdue Biological Safety Committee Agreement
Non-Funded Cooperative Agreement (N)
  Accession Number: 433407
Improvement of Oat Germplasm for Resistance/Tolerance to Barley and Cereal Yellow Dwarf Virus Utilizing Marker-Assisted-Selection Breeding
Non-Assistance Cooperative Agreement (S)
  Accession Number: 433659
Identifying Genes for Resistance to Septoria tritici Blotch and Other Diseases in Wheat
Non-Assistance Cooperative Agreement (S)
  Accession Number: 435533
Ag-Discovery Summer Enrichment Program
Non-Assistance Cooperative Agreement (S)
  Accession Number: 436853
Developing Methods for More Efficient Wheat Transformation
Non-Assistance Cooperative Agreement (S)
  Accession Number: 437046

Publications (Clicking on the reprint icon Reprint Icon will take you to the publication reprint.)
Phenotypic and molecular characterization of Hessian fly resistance in diploid wheat, Aegilops tauschii -
Nemacheck, J.A., Schemerhorn, B.J., Scofield, S.R., Subramanyam, S.N. 2019. Phenotypic and molecular characterization of Hessian fly resistance in diploid wheat, Aegilops tauschii. Biomed Central (BMC) Plant Biology. 19:439. https://doi.org/10.1186/s12870-019-2058-6.
Virus-induced gene silencing (VIGS) for functional characterization of disease resistance genes in barley seedlings -
Gunupuru, L.R., Perochon, A., Ali, S.S., Scofield, S.R., Doohan, F.M. 2018. Virus-induced gene silencing (VIGS) for functional characterization of disease resistance genes in barley seedlings. Methods in Molecular Biology. 1900:95-114. https://doi.org/10.1007/978-1-4939-8944-7_7.
Thymol-based sub-micron emulsions exhibit antifungal activity against Fusarium graminearum and inhibit Fusarium head blight (FHB) in wheat -
Gill, T.A., Li, J., Doppler, M., Scofield, S.R. 2016. Thymol-based sub-micron emulsions exhibit antifungal activity against Fusarium graminearum and inhibit Fusarium head blight (FHB) in wheat. Journal of Applied Microbiology. 4:1103-1116. doi:10.1111/jam.13195.
TaFROG encodes a Pooideae orphan protein that interacts with SnRK1 and enhances resistance to the mycotoxigenic fungus fusarium graminearum. -
Perochon, A., Jianguang, J., Kahla, A., Arunachalam, C., Scofield, S.R., Bowden, S., Wallington, E., Doohan, F. 2015. TaFROG, a novel plant orphan gene, interacts with SnRK1 and enhances resistance to a mycotoxigenic fungus. Plant Physiology. 169(4): 2895-2906. doi: http://dx.doi.org/10.1104/pp.15.01056.
Virus-induced gene silencing (VIGS) in barley seedling leaves -
Gunupuru, L.R., Ali, S.S., Doohan, F.M., Scofield, S.R. 2015. Virus-induced gene silencing (VIGS) in barley seedling leaves. Bio-protocol. 5(12): 1506-8.
A wheat ABC transporter contributes to both grain formation and mycotoxin tolerance -
Walter, S., Kahla, A., Arunachalam, C., Perochon, A., Khan, M.R., Scofield, S.R., Doohan, F.M. 2015. A wheat ABC transporter contributes to both grain formation and mycotoxin tolerance. Journal of Experimental Botany. 66(9):2583-2593. DOI:10.1093/jxb/erv048.
Plant disease resistance is augmented in uzu barley lines modified in the brassinosteroid receptor BRI1 -
Ali, S.S., Gunupuru, L.R., Kumar, G.S., Khan, M., Scofield, S.R., Nicholson, P., Doohan, F.M. 2014. Plant disease resistance is augmented in uzu barley lines modified in the brassinosteroid receptor BRI1. Biomed Central (BMC) Plant Biology. 14:227.
New tools to determine gene function in maize -
Benavente, L.M., Scofield, S.R. 2011. New tools to determine gene function in maize. New Phytologist. 189:363-365.
Intracellular expression of a host-selective toxin, ToxA, in diverse plants phenocopies silencing of a ToxA-interacting protein, ToxABP1 -
Manning, V.A., Chu, A.L., Scofield, S.R., Ciufetti, L.M. 2010. Intracellular expression of a host-selective toxin, ToxA, in diverse plants phenocopies silencing of a ToxA-interacting protein, ToxABP1. New Phytologist. 187:1034-1047.
Virus-induced gene silencing of WRKY53 and an inducible phenylalanine ammonia-lyase in wheat reduces aphid resistance -
Van Eck, L., Schultz, T., Leach, J.E., Scofield, S.R., Peairs, F.B., Botha, A., Lapitan, N.V. 2010. Virus-induced gene silencing of WRKY53 and an inducible phenylalanine ammonia-lyase in wheat reduces aphid resistance. Plant Biotechnology Journal. 9:1023-1032.
Methods for Virus-Induced Gene Silencing in Hexaploid Wheat using barley stripe mosaic virus vectors -
Scofield, S.R., Brandt, A.S. 2012. Methods for Virus-Induced Gene Silencing in Hexaploid Wheat using barley stripe mosaic virus vectors. Methods in Molecular Biology. 894:93-112.
Rapid Determination of Gene Function by Virus-Induced Gene Silencing in Wheat and Barley Reprint Icon -
Scofield, S.R., Gillespie, M., Cakir, C. 2010. Rapid Determination of Gene Function by Virus-Induced Gene Silencing in Wheat and Barley. Crop Science. 50:77-84.
Rapid Determination of Gene Function by Virus-induced Gene Silencing in Wheat and Barley -
Cakir, C., Scofield, S.R., Gillespie, M.E. 2010. Rapid determination of gene function by virus-induced gene silencing in wheat and barley. Crop Science. 50(2, Suppl. S):S77-S84.
Two Genes Encoding Structurally Different CC-NB-LRR Proteins are Required for Lr10-Mediated Leaf Rust Resistance in Wheat of Two Ploidy Levels -
Loutre, C., Wicker, T., Travella, S., Galli, P., Scofield, S.R., Fahima, T., Feuillet, C., Keller, B. 2009. Two Genes Encoding Structurally Different CC-NB-LRR Proteins are Required for Lr10-Mediated Leaf Rust Resistance in Wheat of Two Ploidy Levels. Plant Journal. 60:1043-54.
Functional dissection of wheat disease resistance pathways -
Scofield, S.R. 2009. Functional dissection of wheat disease resistance pathways. Plant Genome Conference Proceedings.
Small-Interfering RNAs from Natural Antisense Transcripts Derived from a Cellulose Synthase Gene Modulate Cell Wall Biosynthesis in Barley -
Held, M.A., Penning, B., Kessans, S.A., Yong, W., Scofield, S.R., Brandt, A.S., Carpita, N.C. 2008. Small-Interfering RNAs from Natural Antisense Transcripts Derived from a Cellulose Synthase Gene Modulate Cell Wall Biosynthesis in Barley. Proceedings of the National Academy of Sciences. 105:20534-10539.
Resources for Virus-Induced Gene Silencing (VIGS) in the Grasses -
Scofield, S.R., Nelson, R. 2009. Resources for Virus-Induced Gene Silencing (VIGS) in the Grasses. Plant Physiology. 149:152-157.
Evaluating the ability of the barley stripe mosaic virus-induced gene silencing system to simultaneously silence two wheat genes -
Cakir, C., Scofield, S.R. 2008. Evaluating the ability of the barley stripe mosaic virus-induced gene silencing system to simultaneously silence two wheat genes. Cereal Research Communications. 36:21-222.
Evaluating the Ability of the Barley Stripe Mosaic Virus-Induced Gene Silencing System to Simultaneously Silence Two Wheat Genes -
Scofield, S.R., Cakir, C. 2008. Evaluating the Ability of the Barley Stripe Mosaic Virus-Induced Gene Silencing System to Simultaneously Silence Two Wheat Genes. Cereal Research Communications. 36:217-222.
Genetics of host-pathogen interactions in the wheat-Stagonospora nodorum pathosystem -
Faris, J.D., Lu, H.J., Zhang, Z., Liu, Z.H., Xu, S.S., Chu, C.G., Abeysekara, N., Fellers, J.P., Cloutier, S., Keller, B., Scofield, S.R., Friesen, T.L. 2008. Genetics of host-pathogen interactions in the wheat-Stagonospora nodorum pathosystem. International Wheat Conference Proceedings. Australia Aug 24-29
A History of Grafting -
Mudge, K., Goldschmidt, E., Scofield, S.R., Janick, J. 2009. A History of Grafting. Horticultural Reviews, Plant Breeding Reviews. 35:437-487.
A guardian of grasses: specific origin and conservation of a unique disease-resistance gene in the grass lineage -
Sindhu, A., Chintamanani, S., Scofield, S.R., Brandt, A.S., Johal, G. 2008. A guardian of grasses: specific origin and conservation of a unique disease-resistance gene in the grass lineage. Journal of the National Academy of Sciences. 105:1762-1767.
Rapid Identification of Genes Contributing to FH Resistance in Wheat -
Scofield, S.R. 2007. Rapid Identification of Genes Contributing to FH Resistance in Wheat. The Canadian Workshop on Fusarium Head Blight. p. 76.
Wheat-rice colinearity and chromosome walking at the Snn1 locus in wheat -
Reddy, L., Friesen, T.L., Meinhardt, S.W., Chao, S., Scofield, S.R., Faris, J.D. 2007. Wheat-rice colinearity and chromosome walking at the Snn1 locus in wheat. Meeting Abstract. National Wheat Genomics Conference Abstracts. pg 28
Genetic analysis of host-toxin interactions in the wheat-Stagonospora nodorum pathosystem -
Faris, J.D., Friesen, T.L., Lu, H., Reddy, L., Zhang, Z., Liu, Z., Xu, S.S., Meinhardt, S.W., Fellers, J.P., Oliver, R.P., Solomon, P.S., Chao, S., Chu, C., Abeysekara, N., Scofield, S.R. 2007. Genetic analysis of host-toxin interactions in the wheat-Stagonospora nodorum pathosystem. Meeting Abstract. National Wheat Genomics Conference abstracts pg. 11
Virus-Induced Gene Silencing in Hexaploid Wheat -
Scofield, S.R., Cakir, C., Brandt, A.S., Kong, L., Anderson, J.M. 2007. Virus-Induced Gene Silencing in Hexaploid Wheat. Plant and Animal Genome VX Conference Abstracts. p. 794.
A VIRUS-INDUCED GENE SILENCING SYSTEM FOR THE ANALYSIS OF DISEASE RESISTANCE PATHWAYS IN WHEAT AND BARLEY -
DEVELOPMENT OF A VIRUS-INDUCED GENE-SILENCING SYSTEM FOR HEXAPLOID WHEAT AND ITS USE IN FUNCTIONAL ANALYSIS OF THE LR21-MEDIATED LEAF RUST RESISTANCE PATHWAY -
Scofield, S.R., Huang, L., Brandt, A.S., Gill, B.S. 2005. Development of a virus-induced gene-silencing system for hexaploid wheat and its use in functional analysis of the lr21-mediated leaf rust resistance pathway. Plant Physiology. 10.1104:105.061861
FUNCTIONAL PATHOGENOMICS IN CEREALS -
Scofield, S.R., Brandt, A.S., Anderson, J.M., Crane, C.F., Goodwin, S.B., Ohm, H., Williams, C.E., Lohret, T., Crasta, O. 2004. Functional pathogenomics in cereals (abstract). Plant Animal and Microbe Genome XII. Abstract No. P839.
FUNCTIONAL PATHOGENOMICS IN CEREALS -
ANALYSIS OF THE WHEAT DEFENSE TRANSCRIPTOME -
Scofield, S.R., Anderson, J.M., Crane, C.F., Goodwin, S.B., Ohm, H., Williams, C.E., Loret, T., Crasta, O. 2003. Analysis of the wheat defense transcriptome. Proceedings of the Tenth International Wheat Genetics Symposium, Paestum, Italy. 1:407-410.
QUANTITATIVE ANALYSIS OF WHEAT DEFENSE-GENE EXPRESSION IN RESPONSE TO INSECT, FUNGAL, AND VIRAL PESTS -
Anderson, J.M., Williams, C.E., Goodwin, S.B., Scofield, S.R., Ohm, H.W. 2003. Quantitative analysis of wheat defense-gene expression in response to insect, fungal, and viral pests. In: Proceedings of the Tenth International Wheat Genetics Symposium, September 1-6, 2003. Paestum, Italy. 1:323-326.
TRANSCRIPTION PROFILING OF WHEAT DEFENSE RESPONSES: HESSIAN FLY VERSUS MICROBIAL PATHOGENS -
Williams, C.E., Goodwin, S.B., Ohm, H.W., Anderson, J.M., Crane, C.F., Scofield, S.R., Lohret, T.A., Crasta, O.R. 2003. Transcription profiling of wheat defense responses: hessian fly versus microbial pathogens. In: Proceedings of the Entomological Society of America Regional Meetings. Entomological Society of American-North Central Branch, March 23-26, 2003, Madison, Wisconsin. Available: http://esa.ent.iastate.edu/confreg/?gridaction=viewonepresentation&year=2003 &presnum=083
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