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United States Department of Agriculture

Agricultural Research Service

Related Topics

George C Paoli
Molecular Characterization of Foodborne Pathogens Research
Microbiology

Phone: (215) 233-6671
Fax: (215) 233-6581
Room 1112

600 E. MERMAID LANE
ERRC
WYNDMOOR , PA 190388598

Projects
INNOVATIVE PATHOGEN DETECTION AND CHARACTERIZATION TECHNOLOGIES FOR USE IN FOOD SAFETY
General Cooperative Agreement (A)
  Accession Number: 423341
Advanced Development of Innovative Technologies & Systematic Approaches to Foodborne Hazard Detection & Characterization for Improving Food Safety
General Cooperative Agreement (A)
  Accession Number: 430589
Development of Portable Detection and Quantification Technologies for Foodborne Pathogens
Appropriated (D)
  Accession Number: 431165
Shiga Toxin-Producing Escherichia coli in Biofilms and within Microbial Communities in Food
Appropriated (D)
  Accession Number: 429656
Advanced Development of Innovative Technologies and Systematic Approaches to Foodborne Hazard Detection and Characterization for Improving Food Safety
Appropriated (D)
  Accession Number: 429773

Publications (Clicking on the reprint icon Reprint Icon will take you to the publication reprint.)
The use of a novel nanoLuc-based reporter phage for the detection of Escherichia coli O157:H7 -
Zhang, D., Coronel-Aguilera, C., Romero, P., Perry, L., Minocha, U., Rosenfield, C., Gehring, A.G., Paoli, G., Bhunia, A.K., Applegate, B. 2016. The use of a novel nanoLuc-based reporter phage for the detection of Escherichia coli O157:H7. Scientific Reports. DOI:10.1038/srep33235.
Synthesis of amino-rich silica coated magnetic nanoparticles and their application in the capture of DNA for PCR -
Bai, Y., Cui, Y., Paoli, G., Shi, C., Wang, D., Shi, X. 2016. Synthesis of amino-rich silica coated magnetic nanoparticles and their application in the capture of DNA for PCR. Colloids and Surfaces B: Biointerfaces. 145:257-266.
Genome-scale screening and validation of targets for identification of Salmonella enterica and serovar prediction -
Zhou, X., Zhang, L., Shi, C., Fratamico, P.M., Liu, B., Paoli, G., Dan, X., Zhuang, X., Cui, Y., Wang, D., Shi, X. 2016. Genome-scale screening and validation of targets for identification of Salmonella enterica and serovar prediction. International Journal of Food Microbiology. 79:376-383.
Nanoparticles affect PCR primarily via surface interactions with PCR components: using amino-modified silica-coated magnetic nanoparticles as a main model -
Bai, Y., Cui, Y., Paoli, G., Shi, C., Wang, D., Shi, X. 2015. Nanoparticles affect PCR primarily via surface interactions with PCR components: using amino-modified silica-coated magnetic nanoparticles as a main model. ACS Applied Materials and Interfaces. DOI:10.1021/am508842v.
Genetically marked strains of Shiga toxin-producing O157:H7 and non-O157 Escherichia coli: Tools for detection and modelling Reprint Icon -
Paoli, G., Uhlich, G.A., Wijey, C. 2015. Genetically marked strains of Shiga toxin-producing O157:H7 and non-O157 Escherichia coli: Tools for detection and modelling. Journal of Food Protection. 78(5):888-901.
Near-quantitative extraction of genomic DNA from various food-borne eubacteria -
Irwin, P.L., Nguyen, L.T., He, Y., Paoli, G., Gehring, A.G., Chen, C. 2014. Near-quantitative extraction of genomic DNA from various food-borne eubacteria. BMC Microbiology. DOI: 10.1186/s12866-014-0326-z.
A high-throughput, precipitating colorimetric sandwich ELISA microarray for shiga toxins Reprint Icon -
Gehring, A.G., He, X., Fratamico, P.M., Lee, J., Bagi, L.K., Brewster, J.D., Paoli, G., He, Y., Xie, Y., Skinner, C.B., Barnett, C., Harris, D. 2014. A high-throughput, precipitating colorimetric sandwich ELISA microarray for shiga toxins. Toxins. 6:1855-1872.
Inactivation of avirulent pgm+ and delta pgm Yersinia pestis by ultraviolet light (UV-C) Reprint Icon -
Paoli, G., Sommers, C.H., Scullen, O.J., Wijey, C. 2014. Inactivation of avirulent pgm+ and delta pgm Yersinia pestis by ultraviolet light (UV-C). Food Microbiology. DOI:10.1016/j.fm.2014.06.002.
Genetically marked strains of Shiga toxin-producing E. coli O157:H7 and non-O157 for detection and modeling -
Paoli, G., Uhlich, G.A., Wijey, C. 2015. Genetically marked strains of Shiga toxin-producing E. coli O157:H7 and non-O157 for detection and modeling. Meeting Abstract. meetingabstract.
Automated immunomagnetic separation for the detection of Escherichia coli O157:H7 from spinach Reprint Icon -
Chen, J., Shi, X., Gehring, A.G., Paoli, G. 2014. Automated immunomagnetic separation of Escherichia coli O157:H7 from spinach. Food Control. 179:33-37.
Detection of E. coli O157:H7 with a reporter phage containing the luxCDABE cassette -
Applegate, B., Rosenfield, C., Martinez, M., Zhu, F., Farrokhzad, K., Paoli, G. 2014. Detection of E. coli O157:H7 with a reporter phage containing the luxCDABE cassette. Meeting Abstract. 1-2.
Phenotypic and genotypic antimicrobial resistance traits of foodborne Staphylococcus aureus isolates from Shanghai Reprint Icon -
Xu, J., Song, M., Yang, P., Shi, C., Paoli, G., Shi, X. 2014. Phenotypic and genotypic antimicrobial resistance traits of foodborne Staphylococcus aureus isolates from Shanghai. Journal of Food Science. 79:635-642.
Casamino acids and oxyrase enhance growth for Listeria monocytogenes in muulti-pathogen enrichments Reprint Icon -
Gehring, A.G., Paoli, G., Reed, S.A., Tu, S., Lindsay, J.A. 2013. Casamino acids and oxyrase enhance growth for Listeria monocytogenes in muulti-pathogen enrichments. Food Control. 40:93-99.
Phenotypic and genotypic characterization of biofilm forming capability in non-O157 Shiga toxin-producing Escherichia coli strains -
Chen, C., Hofmann, C.S., Cottrell, B.J., Strobaugh Jr, T.P., Paoli, G., Nguyen, L.T., Yan, X., Uhlich, G.A. 2013. Phenotypic and genotypic characterization of biofilm forming capability in non-O157 Shiga toxin-producing Escherichia coli strains. PLoS One. 8(12):e84863.
DNA extraction protocol for rapid PCR detection of pathogenic bacteria Reprint Icon -
Brewster, J.D., Paoli, G. 2013. DNA extraction protocol for rapid PCR detection of pathogenic bacteria. Analytical Biochemistry. 442(1):107-109
A rapid method for the detection of foodborne pathogens by extraction of a trace amount of DNA from raw milk based on label-free amino-modified silica-coated magnetic nanoparticles and polymerase chain reaction Reprint Icon -
Bai, Y., Song, M., Cui, Y., Shi, C., Wang, D., Paoli, G., Shi, X. 2013. A rapid method for the detection of foodborne pathogens by extraction of a trace amount of DNA from raw milk based on label-free amino-modified silica-coated magnetic nanoparticles and polymerase chain reaction. Analytica Chimica Acta. 787:93-101.
High-throughput antibody microarray for bacteria and toxins -
A high-throughput antibody-based microarray typing platform -
Gehring, A.G., Barnett, C., Chu, T., Debroy, C., D'Souza, D., Eaker, S., Fratamico, P.M., Gillespie, B., Hedge, N., Jones, K., Lin, J., Oliver, S., Paoli, G., Perera, A., Uknalis, J. 2013. A high-throughput antibody-based microarray typing platform. Sensors. DOI: 10.3390/s130505737.
How does Listeria monocytogenes combat acid conditions? -
Smith, J.L., Liu, Y., Paoli, G. 2012. How does Listeria monocytogenes combat acid conditions?. Canadian Journal of Microbiology. 59:141-152.
Inactivation of F.tularensis Utah-112 on food and food contact surfaces by ultraviolet light -
Sommers, C.H., Scullen, B.J., Paoli, G., Bhaduri, S. 2012. Inactivation of F.tularensis Utah-112 on food and food contact surfaces by ultraviolet light. Journal of Food Processing and Technology. doi:10.4172/2157-7110.S11-002.
Evaluation of fourier transform infrared (FT-IR) spectroscopy and chemometrics as a rapid approach for subtyping E. coli O157:H7 isolates Reprint Icon -
Davis, R., Paoli, G., Mauer, L.J. 2012. Evaluation of fourier transform infrared (FT-IR) spectroscopy and chemometrics as a rapid approach for subtyping E. coli O157:H7 isolates. Food Microbiology. 31:181-190.
A method for correcting standard-based real-time PCR DNA quantitation when the standard's polymerase reaction efficiency is significantly different from that of the unknown's -
Irwin, P.L., Nguyen, L.T., Chen, C., Uhlich, G.A., Paoli, G. 2012. A method for correcting standard-based real-time PCR DNA quantitation when the standard's polymerase reaction efficiency is significantly different from that of the unknown's. Analytical and Bioanalytical Chemistry. 402:2713-2725.
Development and validation of a rapid method for microcystins in fish and comparing LC-MS/MS results with ELISA -
Geis-Asteggiante, L., Lehotay, S.J., Fortis, L.L., Paoli, G., Wijey, C., Heinzen, H. 2011. Development and validation of a rapid method for microcystins in fish and comparing LC-MS/MS results with ELISA. Analytical and Bioanalytical Chemistry. 401:2617-2630.
Emerging foodborne pathogens -
A POPULATION STUDY OF E. COLI DOUBLING TIMES: EVIDENCE OF AN ANOMALOUS BIMODAL DISTRIBUTION BELOW A THRESHOLD CELL CONCENTRATION -
Irwin, P.L., Paoli, G., Chen, C., Nguyen, L.T. 2010. A POPULATION STUDY OF E. COLI DOUBLING TIMES: EVIDENCE OF AN ANOMALOUS BIMODAL DISTRIBUTION BELOW A THRESHOLD CELL CONCENTRATION. BMC Microbiology. 10:207.
UV-C Inactivation of Francisella tularensis Utah-112 on agar surfaces, stainless steel, and foods -
Sommers, C., Paoli, G., 2010. UV-C Inactivation of Francisella tularensis Utah-112 on agar surfaces, stainless steel, and foods [abstract]. Institute of Food Technologists Annual Meeting. Chicago, IL. p.1.
A real-time PCR method for the detection of Salmonella enterica from food using a target sequence identified by comparative genomic analysis -
Chen, J., Zhang, L., Paoli, G., Tu, S., Shi, X. 2010. A real-time PCR method for the detection of Salmonella enterica from food using a target sequence identified by comparative genomic analysis. International Journal of Food Microbiology. 137:168-174.
Evaluation of the MIT RMID 1000 system for the identification of Listeria species:AOAC performance tested method 090325 -
Ricardi, J., Haavig, D., Cruz, L., Paoli, G., Gehring, A.G. 2010. Evaluation of the MIT RMID 1000 system for the identification of Listeria species:AOAC performance tested method 090325. Journal of Association of Official Analytical Chemists International. 93(1):249-258.
Development of an oligonucleotide-based microarray to detect multiple foodborne pathogens -
Suo, B., He, Y., Paoli, G., Gehring, A.G., Tu, S., Shi, X. 2009. Development of an oligonucleotide-based microarray to detect multiple foodborne pathogens. Molecular and Cellular Probes. 24:77-86.
A microarray approach for simultaneous detection of multiple pathogens in food -
Rapid identification of Listeria spp.: an AOAC performance test of the MIT 1000 rapid microbial identification system -
Development of an oligonucleotide-based microarray to detect multiple foodborne pathogens -
Suo, B., He, Y., Paoli, G., Gehring, A.G., Tu, S. 2009. Development of an oligonucleotide-based microarray to detect multiple foodborne pathogens. Meeting Abstract. 1:100.
Production of Shiga-like toxins by Escherichia coli O157:H7: Effects of other bacteria and analogues of quorum sensing molecules -
Tu, S., Uknalis, J., Paoli, G., He, Y., Gehring, A.G. 2009. Production of Shiga-like toxins by Escherichia coli O157:H7: Effects of other bacteria and analogues of quorum sensing molecules. Journal of Rapid Methods and Automation in Microbiology.17(4):420-437.
Capture of E. coli O157:H7 using immunomagnetic beads of different size and antibody conjugating chemistry -
Tu, S., Reed, S.A., Gehring, A.G., He, Y., Paoli, G. 2009. Capture of E. coli O157:H7 using immunomagnetic beads of different size and antibody conjugating chemistry. Sensors. 9:717-730.
Rapid Confirmation of Listeria spp. with the MIT 1000 Microbial Identification System -
Development of a Multiplex PCR Method for Detection of the Genes Encoding 16S rRNA, Coagulase, Methicillin Resistance and Enterotoxins in Staphylococcus aureus -
Fan, Y., Pan, F., Paoli, G., Xiao, Y., Sheng, H., Shi, X. 2008. Development of a Multiplex PCR Method for Detection of the Genes Encoding 16S rRNA, Coagulase, Methicillin Resistance and Enterotoxins in Staphylococcus aureus. Journal of Rapid Methods and Automation in Microbiology.16(4):394-411.
Applications of Immunomagnetic Capture and Time-Resolved Fluorescence Detection for Salmonella Enteritidis in Liquid Eggs -
Tu, S., Gehring, A.G., Paoli, G. 2008. Applications of Immunomagnetic Capture and Time-Resolved Fluorescence Detection for Salmonella Enteritidis in Liquid Eggs. International Society of Optical Engineering (SPIE).6983:1-5 . Optical Engineering.
Innovative applications of bacteriophages in rapid detection and identification of foodborne pathogens -
Paoli, G. 2008. Innovative applications of bacteriophages in rapid detection and identification of foodborne pathogens. Meeting Abstract.
DETECTION OF SALMONELLA ENTERITIDIS IN LIQUID EGGS BY IMMUNOMAGNETIC CAPTURE AND TIME-RESOLVED FLUORESCENCE -
Tu, S., Gehring, A.G., Paoli, G. 2008. Detection of salmonella enteritidis in liquid eggs by immunomagnetic capture and time-resolved fluorescence. Meeting Abstract.Available: http://spie.org/x648.xml?product_id=786848&show/Abstracts=true&origin_id=x648&event_id=795339&start_at=41
Binding of Nontarget Organisms to Anti-Salmonella and Anti-E. coli O157 Immunomagnetic Beads: Most Probable Composition of Background Eubacteria -
Irwin, P.L., Nguyen, L., Chen, C., Paoli, G. 2008. Binding of Nontarget Organisms to Anti-Salmonella and Anti-E. coli O157 Immunomagnetic Beads: Most Probable Composition of Background Eubacteria. Analytical and Bioanalytical Chemistry. 391:525-536.
A coupled anti-L. monocytgenes scFv immunomagnetic bead and PCR method for capture and detection of L. monocytogenes from food -
Paoli, G., Tu, S., Fan, Y., Shi, X. 2008. A coupled anti-L. monocytgenes scFv immunomagnetic bead and PCR method for capture and detection of L. monocytogenes from food . Meeting Abstract.
Multiple Pathogen Detection Using Biosensors: Advancements and Challenges -
Tu, S., Gehring, A.G., Paoli, G. 2007. Multiple Pathogen Detection Using Biosensors: Advancements and Challenges. [Abstract]p.59
SPR Biosensor for the Detection of L. monocytogenes using Phage Displayed Antibody -
Detection of salmonella in liquid eggs by immunomagnetic capture and time-resolved fluorescence -
Tu, S., Gehring, A.G., Paoli, G. 2007. Detection of salmonella in liquid eggs by immunomagnetic capture and time-resolved fluorescence. Journal of Rapid Methods and Automation in Microbiology. 15:107-119
A LISTERIA MONOCYTOGENES-SPECIFIC SINGLE CHAIN ANTIBODY BIDNS TO THE ACTIN POLYMERIZATION PROTEIN -
Paoli, G., Brewster, J.D. 2007. A listeria monocytogenes-specific single chain antibody bidns to the actin polymerization protein. Applied and Environmental Microbiology. 15(2007)77-91.
DEVELOPMENT OF LISTERIA MONOCYTOGENES-SPECIFIC IMMUNOMAGNETIC BEADS USING A SINGLE-CHAIN ANTIBODY FRAGMENT -
Paoli, G., Kleina, L.G., Brewster, J.D. 2007. Development of listeria monocytogenes-specific immunomagnetic beads using a single-chain antibody fragment. Foodborne Pathogens and Disease. 4:74-83
IMMUNOMAGNETIC-ELECTROCHEMILUMINESCENT DETECTION OF ESCHERICHIA COLI 0157 IN GROUND BEEF AFTER PRE-ENRICHMENT IN BRILLIANT GREEN BILE BROTH, MODIFIED ESCHERICHIA COLI BROTH WITH OR WITHOUT NOVOBIOCIN, OR GRAM NEGATIVE BROTH -
Feder, I.E., Wijey, C., Paoli, G., Crawford, C.G., Tu, S. 2007. Immunomagnetic-electrochemiluminescent detection of escherichia coli 0157 in ground beef after pre-enrichment in brilliant green bile broth, modified escherichia coli broth with or without novobiocin, or gram negative broth. Journal of Rapid Methods and Automation in Microbiology. 15:92-106.
SENSITIVE DETECTION OF ESCHERICHIA COLI 0157:H7 BY THE USE OF IMMUNOMAGNETIC AND FLUORESCENT BEADS -
Tu, S., Cooke, P.H., Paoli, G., Gehring, A.G. 2006. Sensitive detection of escherichia coli 0157:h7 by the use of immunomagnetic and fluorescent beads. Meeting Abstract.
OXYGEN CONSUMPTION RATE OF CAMPYLOBACTER JEJUNI DURING GROWTH AND SURVIVAL UNDER VARIOUS OXYGEN LEVELS -
Chen, C., Paoli, G., Irwin, P.L. 2006. Oxygen consumption rate of campylobacter jejuni during growth and survival under various oxygen levels. {Abstract}. International Association of Food Protection. P4-59.
IDENTIFICATION OF THE SURFACE ANTIGEN RECOGNIZED BY A LISTERIA MONOCYTOGENES-SPECIFIC PHAGE-DISPLAYED ANTIBODY FRAGMENT AND ITS PRESENCE IN DIFFERENT PHYSIOLOGICAL CONDITIONS -
Paoli, G., Brewster, J.D. 2007. Identification of the surface antigen recognized by a listeria monocytogenes-specific phage-displayed antibody fragment and its presence in different physiological conditions. Journal of Rapid Methods and Automation in Microbiology. Vol. 4(1):74-83.
DETECTION OF ESCHERICHIA COLI 0157:H7 THROUGH THE FORMATION OF SANDWICHED COMPLEXES WITH IMMUNOMAGNETIC AND FLUORESCENT BEADS -
Tu, S., Golden, M., Cooke, P.H., Luce, L., Paoli, G., Gehring, A.G.2005 Detection of escherichia coli 0157:h7 through the formation of sandwiched complexes with immunomagnetic and fluorescent beads. Journal of Rapid Methods and Automation in Microbiology. 13:269-282
LISTERIA MONOCYTOGENES -
Paoli, G., Bhunia, A., Bayles, D. 2005. Listeria monocytogenes. Foodborne Pathogens: Microbiology and Molecular Biology Reviews. Horizon Scientific Press, Norwich, UK. Chapter 15. p. 295-325.
BIOSENSOR-BASED PROCESSES FOR DETECTION OF BACTERIA IN THE FOOD PROCESSING ENVIRONMENT -
Tu, S., Gehring, A.G., Paoli, G. 2005. Biosensor-based processes for detection of bacteria in the food processing environment. International Food Technology Meeting Abstracts. Paper No. 6-4.
DEVELOPMENT OF BIOSENSORS FROM PHAGE DISPLAYED ANTIBODIES -
Paoli, G., Brewster, J.D., Kleina, L.G. 2004. Development of biosensors from phage displayed antibodies. UJNR Food & Agricultural Panel Proceedings. pp. 359-364.
TIME-RESOLVED FLUORESCENCE DETECTION OF TOXINS AND PATHOGENIC BACTERIA IN FOODS -
Tu, S., Golden, M., Paoli, G., Gore, M., Gehring, A.G. 2004. Time-resolved fluorescence detection of toxins and pathogenic bacteria in foods. Journal of Rapid Methods and Automation in Microbiology. 12. pp. 247-258.
DETECTION OF VIABLE BACTERIA CELLS BY BIOLUMINESCENCE: A BIOENERGETIC APPROACH -
Tu, S., Wijey, C., Paoli, G., Gehring, A.G., Irwin, P.L. 2004. Detection of viable bacteria cells by bioluminescence: a bioenergetic approach. Journal of Rapid Methods and Automation in Microbiology. V.12, #3, p. 207-219.
DEVELOPMENT OF BIOSENSORS FOR DETECTION OF FOOD-BORNE PATHOGENS BY ANTIBODY PHAGE -
Paoli, G., Brewster, J.D. 2004. Development of biosensors for detection of food-borne pathogens by antibody phage. (Meeting Abstract). 68(1):146-149.
IMMUNOLOGICAL BIOSENSOR-BASED METHODS -
Paoli, G., Brewster, J.D. 2004. Immunological biosensor-based methods. American Chemical Society Abstracts.
TIME-RESOLVED FLUORESCENCE DETECTION OF SHIGA-LIKE TOXINS PRODUCED BY ESCHERICHIA COLI 0157 AND NON-0157 IN GROUND BEEF -
Tu, S., Golden, M., Paoli, G., Gehring, A.G., Gore, M. 2004. Time-resolved fluorescence detection of shiga-like toxins produced by escherichia coli 0157 and non-0157 in ground beef. Meeting Abstract.
SINGLE CHAIN ANTIBODY WITH SPECIFICITY FOR LISTERIA MONOCYTOGENES -
Paoli, G., Chen, C., Brewster, J.D. 2004. Single chain antibody with specificity for listeria monocytogenes. Journal of Immunological Methods.Vol 289. page 147-155.
CHARACTERIZATION OF A LISTERIA MONOCYTOGENES SPECIES-SPECIFIC SINGLE-CHAIN PHAGE ANTIBODY -
Paoli, G., Brewster, J.D. 2004. Characterization of a listeria monocytogenes species-specific single-chain phage antibody. American Society for Microbiology Annual Meeting.
EFFECT OF BACTERIAL PROTEIN EXTRACTION REAGENT AND SODIUM AZIDE ON THE DETECTION OF ESCHERICHIA COLI 0157:H7 USING IMMUNOMAGNETIC-ELECTROCHEMILUMINESCENCE -
Wijey, C., Crawford, C.G., Tu, S., Paoli, G. Effect of bacterial protein extraction reagent and sodium azide on the detection of escherichia coli 0157:h7 using immunomagnetic-electrochemiluminescence. Journal of Rapid Methods and Automation in Microbiology. 2004. 12. pp. 115-126.
SELECTIVE ISOLATION OF LISTERIA MONOCYTOGENES FROM FOODS USING A SINGLE-CHAIN ANTIBODY FRAGMENT DERIVED BY PHAGE DISPLAY -
Brewster, J.D., Paoli, G. 2004. Selective isolation of listeria monocytogenes from foods using a single-chain antibody fragment derived by phage display. Meeting Abstract. Granada, Spain. Paper #4.
BIOSENSORS FOR THE DETECTION OF FOOD-BORNE PATHOGENS -
Paoli, G., Tu, S. 2003. Biosensors for the detection of food-borne pathogens. Eastern Food Science Conference.
ARS News Articles
Last Modified: 5/2/2016
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