Page Banner

United States Department of Agriculture

Agricultural Research Service

Related Topics

William L Schneider (Bill)
Foreign Disease-weed Science Research
Plant Pathology

Phone: (301) 619-7312
Fax: (301) 619-2880
Room 139

1301 DITTO AVENUE
BLDG 1301
FORT DETRICK , MD 217025023

Projects
Identification, Characterization, and Biology of Foreign and Emerging Viral and Bacterial Plant Pathogens
Appropriated (D)
  Accession Number: 422773
Determining the Role for a Novel Virus in Citrus Blight
Reimbursable (R)
  Accession Number: 427836
Development of PCR Based Diagnostic Tools for Detection and Differentiation of Citurs Leprosis Associated Viruses
Reimbursable (R)
  Accession Number: 430767
Developing RNAseq Diagnostics for Eukaryotic Pathogens of Plants
Specific Cooperative Agreement (S)
  Accession Number: 426498

Publications (Clicking on the reprint icon Reprint Icon will take you to the publication reprint.)
Adaptation and validation of E-probe diagnostic nucleic acid analysis for detection of Escherichia coli O157:H7 in metagenomic data of complex food matrices Reprint Icon -
Blagden, T., Schneider, W.L., Melcher, U., Daniels, J., Fletcher, J. 2016. Adaptation and validation of E-probe diagnostic nucleic acid analysis for detection of Escherichia coli O157:H7 in metagenomic data of complex food matrices. Journal of Food Protection. 79:574-581.
Citrus blight research -
Schneider, W.L., Roy, A., Hartung, J.S., Shao, J.Y., Brlansky, R. 2015. Citrus blight research. Citrus Industry. page 12-14.
Citrus leprosis research update -
Brlansky, R.H., Roy, A., Schneider, W.L., Hartung, J.S. 2015. Citrus leprosis research update. Citrus Industry. 20-22.
History and diversity of Citrus leprosis virus recorded in herbarium specimens Reprint Icon -
Hartung, J.S., Roy, A., Fu, S., Shao, J.Y., Schneider, W.L., Brlansky, R.H. 2015. History and diversity of Citrus leprosis virus recorded in herbarium specimens. Phytopathology. 105:1277-1284.
Insect E-probe Diagnostic Nucleic acid Analysis (EDNA): the application of a novel bioinformatic tool to detection of vectors and pathogens in individual insect and simulated insect trap metagenomes -
Schneider, W.L., Stone, A.L., Sherman, D.J., Wight-Malapi, M., Andreason, S.A., Daniels, J., Ochoa Corona, F., Crouch, J., Wayadande, A. 2015. Insect E-probe Diagnostic Nucleic acid Analysis (EDNA): the application of a novel bioinformatic tool to detection of vectors and pathogens in individual insect and simulated insect trap metagenomes. Phytopathology. 105: S4:124.
Protein expression profiling of Rathayibacter toxicus using mass spectrometry -
Fennessey, C.M., Blanc, J., Sechler, A.J., Mcmahon, M.B., Garrett, W.M., Luster, D.G., Schneider, W.L. 2015. Protein expression profiling of Rathayibacter toxicus using mass spectrometry. Phytopathology. 105:S4:43.
Role bending: complex relationships between viruses, hosts and vectors related to citrus leprosis, an emerging disease Reprint Icon -
Roy, A., Hartung, J.S., Schneider, W.L., Shao, J.Y., Leon, G.M., Melzer, M.J., Beard, J.J., Otero-Colina, G., Bauchan, G.R., Ochoa, R., Brlansky, R.H. 2015. Role bending: complex relationships between viruses, hosts and vectors related to citrus leprosis, an emerging disease. Phytopathology. DOI: 10.1094/PHYTO-12-14-0375-FI.
Identification and molecular characterization of nuclear Citrus leprosis virus, an unassigned Dichorhavirus genus member associated with citrus leprosis disease in Mexico -
Roy, A., Stone, A.L., Shao, J.Y., Colina, G., Wei, G., Choudary, N., Achor, D., Nakhla, M., Levy, L., Hartung, J.S., Schneider, W.L., Brlansky, R. 2015. Identification and molecular characterization of nuclear Citrus leprosis virus, an unassigned Dichorhavirus genus member associated with citrus leprosis disease in Mexico. Virus Research. 105:564-575.
Metagenomic search strategies for interactions among plants and multiple microbes -
Melcher, U.K., Verma, R., Schneider, W.L. 2014. Metagenomic search strategies for interactions among plants and multiple microbes. Frontiers in Plant Science. DOI: 10.3389/fpls.2014.00268.
Screening metagenomic data for viruses using the E-Probe Diagnostic Nucleic Acid Assay (EDNA) -
Stobbe, A.H., Schneider, W.L., Hoyt, P., Melcher, U. 2014. Screening metagenomic data for viruses using the E-Probe Diagnostic Nucleic Acid Assay (EDNA). Phytopathology. 104:1125-1129.
First report of citrus leprosis virus nuclear type in Colombia -
Roy, A., Leon, M., Stone, A.L., Schneider, W.L., Hartung, J.S., Brlansky, R. 2014. First report of citrus leprosis virus nuclear type in Colombia. Plant Disease. 98:1162.
A population of endogenous pararetrovirus genomes in carrizo citrange Reprint Icon -
Roy, A., Shao, J.Y., Schneider, W.L., Hartung, J.S., Brlansky, R.H. 2013. A population of endogenous pararetrovirus genomes in carrizo citrange. Genome Announcements. 2(1):e01063-13.
E-probe Diagnostic Nucleic acid Analysis (EDNA): A theoretical approach for handling of next generation sequencing data for diagnostics -
Stobbe, A., Daniels, J., Espindola, A., Verma, R., Melcher, U., Ochoa-Corona, F., Garzon, C., Fletcher, J., Schneider, W.L. 2013. E-probe Diagnostic Nucleic acid Analysis (EDNA): A theoretical approach for handling of next generation sequencing data for diagnostics. Journal of Microbiological Methods. 94:356-366.
Genome assembly of citurs leprosis virus nuclear type reveals a close association with orchid fleck virus Reprint Icon -
Roy, A., Choudary, N., Colina, G., Stone, A.L., Shao, J.Y., Levy, L., Nakhla, M., Hollingsworth, C., Hartung, J.S., Schneider, W.L., Brlansky, R. 2013. Genome assembly of citurs leprosis virus nuclear type reveals a close association with orchid fleck virus. Genome Announcements. doi:10.1128/genomeA.00519-13.
A case study on discovery of novel Citrus leprosis virus cytoplasmic type 2 utilizing small RNA libraries by next generation sequencing and bioinformatic analyses Reprint Icon -
Roy, A., Shao, J.Y., Hartung, J.S., Schneider, W.L., Brlansky, R. 2013. A case study on discovery of novel Citrus leprosis virus cytoplasmic type 2 utilizing small RNA libraries by next generation sequencing and bioinformatic analyses. Journal of Data Mining in Genomics & Proteomics. 4:129. doi: 10.4172/2153-0602.1000129.
Development of primers and probes for genus and species specific detection of 'Candidatus Liberibacter species' by real-time PCR Reprint Icon -
Ananthakrishnan, G., Choudhary, N., Roy, A., Sengoda, V.G., Postnikova, E.N., Hartung, J.S., Stone, A.L., Damsteegt, V.D., Schneider, W.L., Munyaneza, J.E., Brlansky, R.H. 2013. Development of primers and probes for genus and species specific detection of 'Candidatus Liberibacter species' by real-time PCR. Plant Disease. 97:1235-1243.
A previously undescribed potyvirus isolated and characterized from arborescent Brugmansia Reprint Icon -
Damsteegt, V.D., Stone, A.L., Smith, O.P., Mcdaniel, L., Sherman, D.J., Dardick, C.D., Hammond, J., Jordan, R.L., Schneider, W.L. 2013. A previously undescribed potyvirus isolated and characterized from arborescent Brugmansia. Archives of Virology. 158:1235-1244.
Molecular analysis of Soybean dwarf virus isolates in the eastern United States confirms the presence of both D and Y strains and provides evidence of mixed infections and recombination -
Schneider, W.L., Damsteegt, V.D., Stone, A.L., Kuhlman, M., Bunyard, B., Sherman, D.J., Graves, M., Smythers, G., Smith, O., Hatziloukas, E. 2011. Molecular analysis of Soybean dwarf virus isolates in the eastern United States confirms the presence of both D and Y strains and provides evidence of mixed infections and recombination. Virology. 412: 46-54.
Molecular, ultrastructural and biological characterization of Pennsylvania isolates of plum pox potyvirus (PPV) -
Schneider, W.L., Damsteegt, V.D., Gildow, F.E., Stone, A.L., Sherman, D.J., Levy, L.E., Mavrodieva, V., Richwine, N., Welliver, R., Luster, D.G. 2011. Molecular, ultrastructural and biological characterization of Pennsylvania isolates of plum pox potyvirus (PPV). Phytopathology. 94:528-533.
Acquistion and transmissibility of United States Soybean dwarf virus isolates by the soybean aphid Aphis glycines -
Damsteegt, V.D., Stone, A.L., Kuhlmann, M., Gildow, F.E., Domier, L.L., Sherman, D.J., Tian, B., Schneider, W.L. 2011. Acquistion and transmissibility of United States Soybean dwarf virus isolates by the soybean aphid Aphis glycines. Plant Disease. 95: 945-950.
Analysis of visual symptomatology in peach and plum inoculated with U.S. PPV isolates -
Younkins, A.A., Stone, A.L., Sherman, D.J., Schneider, W.L., Scorza, R., Damsteegt, V.D. 2010. Analysis of visual symptomatology in peach and plum inoculated with U.S. PPV isolates. Phytopathology. 100:S209.
Development of primers and probes for detection of citrus "Candidatus Liberibacter species" by real-time PCR -
Govindarajulu, A., Choudhary, N., Hartung, J.S., Schneider, W.L., Brlansky, R.H. 0200. Development of primers and probes for detection of citrus "Candidatus Liberibacter species" by real-time PCR. Phytopathology 100(6):S42.
The relevance of Murraya paniculata and related species as potential hosts and inoculum reservoirs of Candidatus Liberibacter asiaticus, causal agent of Huanglongbing (HLB) -
Damsteegt, V.D., Postnikova, E.N., Stone, A.L., Kuhlmann, M., Wilson, C., Sechler, A.J., Schaad, N.W., Brlansky, R.H., Schneider, W.L. 2010. The relevance of Murraya paniculata and related species as potential hosts and inoculum reservoirs of Candidatus Liberibacter asiaticus, causal agent of Huanglongbing (HLB). Plant Disease. 94:528-533.
An improved method of DNA extraction from Diaphorina citri for HLB detection -
Postnikova, E.N., Stone, A.L., Wilson, C.M., Sherman, D.J., Sechler, A.J., Schuenzel, E., Schaad, N.W., Schneider, W.L., Damsteegt, V.D. 2009. An improved method of DNA extraction from Diaphorina citri for HLB detection. Phytopathology. 99:S104.
Preliminary evidence for mixed populations of Ca. Liberibacter species in Huanglongbing infections -
Postnikova, E.N., Stone, A.L., Wilson, C.M., Sherman, D.J., Sechler, A.J., Schuenzel, E., Schaad, N.W., Schneider, W.L., Damsteegt, V.D. 2009. Preliminary evidence for mixed populations of Ca. Liberibacter species in Huanglongbing infections. Phytopathology. 99:S205.
Rathayibacter iranicus isolated from asymptomatic wheat seeds in Turkey -
Postnikova, E.N., Agarkova, I., Altundag, S., Eskandari, F., Sechler, A.J., Karahan, A., Vidaver, A.K., Schneider, W.L., Ozakman, M., Schaad, N.W. 2009. Rathayibacter iranicus isolated from asymptomatic wheat seeds in Turkey. Plant Pathology. 58:796.
Cultivation of Candidatus Liberibacter asiaticus and Ca. L. americanus associated with Huanglongbing -
Sechler, A.J., Schuenzel, E., Cooke, P.H., Donnua, S., Thaveechai, N., Postnikova, E.N., Stone, A.L., Schneider, W.L., Damsteegt, V.D., Schaad, N.W. 2009. Cultivation of Candidatus Liberibacter asiaticus and Ca. L. americanus associated with Huanglongbing. Phytopathology. 99:480-486.
Identification of bacterial plant pathogens using multilocus PCR and electrospray ionization-mass spectrometry (PCR/ESI-MS) -
Postnikova, E.N., Baldwin, C., Whitehouse, C., Sechler, A.J., Schaad, N.W., Sampath, R., Harpin, V., Li, F., Melton, R., Blynn, L., Drader, J., Hofstadler, S., Schneider, W.L. 2008. Identification of bacterial plant pathogens using multilocus PCR and electrospray ionization-mass spectrometry (PCR/ESI-MS). Phytopathology. 98-11-1156-1164
Fidelity of a viral RNA polymerase in an intact host: template structure and host factors affect rates of deletion mutations -
Pita, J., De Miranda, J., Schneider, W.L., Roossinck, M.J. 2007. Fidelity of a viral RNA polymerase in an intact host: template structure and host factors affect rates of deletion mutations. Journal of Virology. 81:9072-9077.
IDENTIFICATION OF A MUTATION IN THE PLUM POX POTYVIRUS NIB GENE ASSOCIATED WITH ADAPTATION TO PEA (PISUM SATIVUM) -
Wallis, C.M., Schneider, W.L., Stone, A.L., Sherman, D.J., Damsteegt, V.D., Gildow, F.E. 2007. Identification of a mutation in the plum pox potyvirus nib gene associated with adaptation to pea (pisum sativum). Journal of General Virology. 88:2839-2845.
Murraya paniculata (orange jasmine), a host and possible inoculum reservoir for Candidatus Liberibacter asiaticus, causal agent of Huanglongbing -
Damsteegt, V.D., Schneider, W.L., Stone, A.L. 2007. Murraya paniculata (orange jasmine), a host and possible inoculum reservoir for Candidatus Liberibacter asiaticus, causal agent of Huanglongbing. Phytopathology. 97:S26 . Phytopathology.
A comprehensive Prunus pathogen array -
Schneider, W.L., Sherman, D.J. 2007. A comprehensive Prunus pathogen array. Phytopathology. 97:S105
PRUNUS HOST RANGE OF PLUM POX VIRUS (PPV) IN THE UNITED STATES BY APHID AND GRAFT INOCULATION -
Damsteegt, V.D., Scorza, R., Stone, A.L., Schneider, W.L., Webb, K.K., Demuth, M.A., Gildow, F.E. 2007. Prunus host range of plum pox virus (ppv) in the United States by aphid and graft inoculation. Plant Disease. 91:18-23
IDENTIFICATION, CHARACTERIZATION AND USE OF A BROME MOSAIC VIRUS STRAIN AS A VECTOR FOR GENE SILENCING IN MONOCOTYLEDONOUS HOSTS -
Ding, X.S., Schneider, W.L., Chaluvadi, S., Mian, M.R., Nelson, R.S. 2006. Identification, characterization and use of a brome mosaic virus strain as a vector for gene silencing in monocotyledonous hosts. Molecular Plant-Microbe Interactions. MPMI 19:1229-1239
ANALYSIS OF GENETIC BOTTLENECKS DURING HORIZONTAL TRANSMISSION OF CUCUMBER MOSAIC VIRUS -
Ali, A., Li, H., Schneider, W.L., Sherman, D.J., Gray, S.M., Smith, D., Roossinck, M.J. 2006. Analysis of genetic bottlenecks during horizontal transmission of cucumber mosaic virus. Journal of Virology. 80:8345-8350
Synthesis of minus-strand copies of a viral transgene during viral infections of transgenic plants -
Deng, M., Schneider, W.L., Allison, R. 2006. Synthesis of minus-strand copies of a viral transgene during viral infections of transgenic plants. Virus Research.10.1016/ 2006.05.009.
QUANTIFICATION OF POSITIVE AND NEGATIVE STRAND RATIOS FOR PLUM POX VIRUS BY REAL-TIME FLUORESCENT REVERSE TRANSCRIPTION-PCR -
IN PLANTA INTERACTIONS OF THREE PLUM POX POTYVIRUS STRAINS WITHIN CULTIVAR LOVELL PEACH SEEDLINGS -
Damsteegt, V.D., Stone, A.L., Sherman, D.J., Schneider, W.L., Gildow, F., Luster, D.G. 2005. In planta interactions of three plum pox potyvirus strains within cultivar Lovell peach seedlings[abstract]. Phytopathology. 95:S22.
PLUM POX POTYVIRUS POPULATION DIVERSITY IN PENNSYLVANIA, IN SINGLE ORCHARDS, AND IN INDIVIDUAL REPLICATING POPULATIONS -
Schneider, W.L., Sherman, D.J., Stone, A.L., Buckley, K., Damsteegt, V.D. 2005. Plum pox potyvirus population diversity in Pennsylvania, in single orchards, and in individual replicating populations [abstract]. Phytopathology. 95:S94.
THE SOYBEAN APHID, APHIS GLYCINES, A NEW VECTOR OF ENDEMIC DWARFING AND YELLOWING ISOLATES OF SOYBEAN DWARF LUTEOVIRUS -
Damsteegt, V.D., Stone, A.L., Schneider, W.L., Sherman, D.J., Gildow, F.E., Luster, D.G. 2005. The soybean aphid, Aphis glycines, a new vector of endemic dwarfing and yellowing isolates of soybean dwarf luteovirus [abstracr]. Phytopathology. 95:S22.
TRANSMISSION OF TWO NORTH AMERICAN ISOLATES OF PLUM POX VIRUS: IDENTIFICATION OF APHID VECTORS AND SPECIES-SPECIFIC TRANSMISSION FROM INFECTED STONE FRUITS -
Gildow, F.E., Damsteegt, V.D., Stone, A.L., Schneider, W.L., Luster, D.G., Levy, L. 2004. Transmission of two north american isolates of plum pox virus: identification of aphid vectors and species-specific transmission from infected stone fruits. Acta Horticulture 657:207-212
POTENTIAL PRUNUS HOST RANGE OF PPV-PENN ISOLATES BY APHID TRANSMISSION -
Damsteegt, V.D., Stone, A.L., Gildow, F.E., Schneider, W.L., Luster, D.G. 2004. Potential prunus host range of ppv-penn isolates by aphid transmission. Acta Horticulture 657:201-206
TRANSMISSION OF THREE NORTH AMERICAN ISOLATES OF PLUM POX VIRUS: IDENTIFICATION OF APHID VECTORS AND SPECIES-SPECIFIC TRANSMISSION FROM INFECTED STONE FRUITS -
Gildow, F., Damsteegt, V.D., Stone, A.L., Schneider, W.L., Luster, D.G., Levy, L. 2004. Transmission of three north american isolates of plum pox virus: identification of aphid vectors and species-specific transmission from infected stone fruits. Acta Horticulture 657:207-212
DETECTION AND QUANTIFICATION OF PLUM POX POTYVIRUS IN APHID VECTORS BY REAL-TIME FLUORESCENT REVERSE TRANSCRIPTION-PCR -
Acta Horticulture 657:135-140
PLUM POX IN NORTH AMERICA: IDENTIFICATION OF APHID VECTORS AND A ROLE FOR FRUIT IN VIRUS SPREAD -
Gildow, F., Damsteegt, V.D., Stone, A.L., Schneider, W.L., Luster, D.G., Levy, L. 2004. Plum pox in north america: identification of aphid vectors and a role for fruit in virus spread. Phytopathology. 94:868-874.
SPECIFIC DETECTION AND QUANTIFICATION OF PLUM POX POTYVIRUS BY REAL-TIME FLUORESCENT REVERSE TRANSCRIPTION-PCR -
Schneider, W.L., Sherman, D.J., Stone, A.L., Damsteegt, V.D., Frederick, R.D. 2004. Specific detection and quantification of plum pox potyvirus by real-time fluorescent reverse transcription-PCR. Journal of Virological Methods.120:97-105.
COMPARATIVE HOST RANGE OF U.S. ISOLATES OF PLUM POX VIRUS AMONG PRUNUS AND OTHER WOODY PLANT SPECIES FOLLOWING GRAFT INOCULATION OR APHID TRANSMISSION -
Damsteegt, V.D., Scorza, R., Gildow, F.E., Schneider, W.L., Stone, A.L., Luster, D.G. 2004. Comparative host range of U.S. isolates of plum pox virus among Prunus and other woody plant species following graft inoculation or aphid transmission. Phytopathology. 94:S24.
DETECTION AND QUANTIFICATION OF PLUM POX POTYVIRUS DOUBLE-STRANDED RNA WITH REAL-TIME PCR -
Schneider, W.L., Stone, A.L., Sherman, D.J., Damsteegt, V.D. 2004. Detection and quantification of plum pox potyvirus double-stranded RNA with real-time PCR. Phytopathology. 94:S93.
HOST ADAPTATION AND MEMORY IN PLUM POX POTYVIRUS POPULATIONS -
Schneider, W.L., Stone, A.L., Wallis, C., Sherman, D.J., Shank, K., Damsteegt, V.D., Gildow, F. 2003. Host adaptation and memory in plum pox potyvirus populations. Phytopathology 93:S100.
POTENTIAL PRUNUS HOST RANGE OF PPV-PENN ISOLATES BY APHID TRANSMISSION -
Damsteegt, V.D., Stone, A.L., Gildow, F.E., Schneider, W.L., Luster, D.G. 2004. Potential prunus host range of ppv-penn isolates by aphid transmission. Acta Horticulture Proceedings. p. 46.
ADVANCES IN MOLECULAR-BASED DIAGNOSTICS IN MEETING CROP BIOSECURITY AND PHYTOSANITARY ISSUES -
SCHAAD, N.W., FREDERICK, R.D., SHAW, J., SCHNEIDER, W.L., HICKSON, R., PETRILLO, M.D., LUSTER, D.G. ADVANCES IN MOLECULAR-BASED DIAGNOSTICS IN MEETING CROP BIOSECURITY AND PHYTOSANITARY ISSUES. ANNUAL REVIEW OF PHYTOPATHOLOGY. 41: . 2003.
SPECIFIC DETECTION AND QUANTIFICATION OF PLUM POX POTYVIRUS BY REAL-TIME FLUORESCENT RT-PCR -
FIELD PERFORMANCE OF CUCURBIT AND TOMATO PLANTS COLONIZED WITH A NONPATHOGENIC, MUTUALISTIC MUTANT (PATH-1) OF COLLETOTRICHUM MAGNA (TELEOMORPH: GLOMERELLA MAGNA; JENKINS AND WINSTEAD) -
VIRAL RNA QUASISPECIES GENERATED BY TRANSGENICALLY-EXPRESSED VIRAL REPLICASE -
ARS News Articles
Last Modified: 5/2/2016
Footer Content Back to Top of Page