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United States Department of Agriculture

Agricultural Research Service

Related Topics

Peter L Irwin
Molecular Characterization of Foodborne Pathogens Research
Microbiology

Phone: (215) 233-6420
Fax: (215) 233-6581
Room 1116.2

600 E MERMAID LN
ERRC
WYNDMOOR , PA 190388598

Projects
Shiga Toxin-Producing Escherichia coli in Biofilms and within Microbial Communities in Food
In-House Appropriated (D)
  Accession Number: 429656

Publications (Clicking on the reprint icon Reprint Icon will take you to the publication reprint.)
Study on the mechanism of antibacterial action of magnesium oxide nanoparticles against foodborne pathogens -
He, Y., Ingudam, S., Reed, S.A., Gehring, A.G., Strobaugh Jr, T.P., Irwin, P.L. 2016. Study on the mechanism of antibacterial action of magnesium oxide nanoparticles against foodborne pathogens. Journal of Nanobiotechnology (Biomed Central Open Access). DOI:10.1186/s12951-016-0202-0.
Multiple mechanisms responsible for strong Congo red-binding variants of Escherichia coli O157:H7 strains -
Chen, C., Nguyen, L.T., Cottrell, B.J., Irwin, P.L., Uhlich, G.A. 2015. Multiple mechanisms responsible for strong Congo red-binding variants of Escherichia coli O157:H7 strains. Pathogens and Disease. 74:123.
Antimicrobial properties and mechanism of magnesium oxide nanoparticles on Campylobacter, E. coli O157:H7, and Salmonella -
Reed, S.A., Ingudum, S., Irwin, P.L., He, Y. 2015. Antimicrobial properties and mechanism of magnesium oxide nanoparticles on Campylobacter, E. coli O157:H7, and Salmonella. Meeting Abstract. meeting abstract.
Complete genome sequence of Campylobacter jejuni YH001 from beef liver which contains a novel plasmid -
He, Y., Yan, X., Reed, S.A., Xie, Y., Chen, C., Irwin, P.L. 2015. Complete genome sequence of Campylobacter jejuni YH001 from beef liver which contains a novel plasmid. Genome Announcements. 3(1):e01492-14.
Near-quantitative extraction of genomic DNA from various food-borne eubacteria -
Irwin, P.L., Nguyen, L.T., He, Y., Paoli, G., Gehring, A.G., Chen, C. 2014. Near-quantitative extraction of genomic DNA from various food-borne eubacteria. BMC Microbiology. DOI: 10.1186/s12866-014-0326-z.
Rapid identification and classification of Campylobacter spp. using laser optical scattering technology Reprint Icon -
He, Y., Reed, S.A., Bhunia, A.K., Gehring, A.G., Nguyen, L.T., Irwin, P.L. 2015. Rapid identification and classification of Campylobacter spp. using laser optical scattering technology. Food Microbiology. 47:28-35.
Variability in DNA polymerase efficiency: effects of random error, DNA extraction method, and isolate type -
Irwin, P.L., Nguyen, L.T., Chen, C., He, Y. 2014. Variability in DNA polymerase efficiency: effects of random error, DNA extraction method, and isolate type . JSM Mathematics & Statistics. 1(1):1003.
High-throughput antibody microarray for bacteria and toxins -
Optimization and application of a custom microarray for the detection and genotyping of E. coli O157:H7 in fresh meat samples Reprint Icon -
Suo, B., He, Y., Irwin, P.L., Gehring, A.G. 2013. Optimization and application of a custom microarray for the detection and genotyping of E. coli O157:H7 in fresh meat samples. Journal of Food Analytical Methods. 6:1477-1484.
Comparison of antibodies raised against heat-and gamma radiation-killed bacteria -
Gehring, A.G., Boyd, G., Brewster, J.D., Irwin, P.L., Thayer, D.W., Van Houten, L.J. 2012. Comparison of antibodies raised against heat-and gamma radiation-killed bacteria. Journal of Microbial and Biochemical Technology. DOI 10.4172/1948-5948.S2-004.
Non-stochastic sampling error in quantal analyses for Campylobacter species on poultry products Reprint Icon -
Irwin, P.L., Reed, S.A., Brewster, J.D., Nguyen, L.T., He, Y. 2013. Non-stochastic sampling error in quantal analyses for Campylobacter species on poultry products. Analytical and Bioanalytical Chemistry. 405(7):2353-2369.
Peroxide resistance in Escherichia coli serotype O157:H7 biofilms is regulated by both RpoS dependent and independent mechanisms -
Uhlich, G.A., Chen, C., Cottrell, B.J., Irwin, P.L., Phillips, J.G. 2012. Peroxide resistance in Escherichia coli serotype O157:H7 biofilms is regulated by both RpoS dependent and independent mechanisms. Microbiology. 158:2225-2234.
Understanding the role of the catalase/peroxide genes in H2O2 resistance of E. coli serotype O157:H7 biofilms -
Uhlich, G.A., Chen, C., Irwin, P.L. 2012. Understanding the role of the catalase/peroxide genes in H2O2 resistance of E. coli serotype O157:H7 biofilms [abstract]. 1:1.
A method for correcting standard-based real-time PCR DNA quantitation when the standard's polymerase reaction efficiency is significantly different from that of the unknown's -
Irwin, P.L., Nguyen, L.T., Chen, C., Uhlich, G.A., Paoli, G. 2012. A method for correcting standard-based real-time PCR DNA quantitation when the standard's polymerase reaction efficiency is significantly different from that of the unknown's. Analytical and Bioanalytical Chemistry. 402:2713-2725.
Keratin capped silver nanoparticles - synthesis and characterization of a nanomaterial with desirable handling properties Reprint Icon -
Martin, J.J., Cardamone, J.M., Irwin, P.L., Brown, E.M. 2011. Keratin capped silver nanoparticles - synthesis and characterization of a nanomaterial with desirable handling properties. Colloids and Surfaces B: Biointerfaces. 88(1):354-361.
Antimicrobial activity of spherical silver nanoparticles: evidence for induction of a prolonged bacterial lag phase -
Antibacterial activity and mechanism of action of zinc oxide nanoparticles against Campylobacter jejuni -
Xie, Y., He, Y., Irwin, P.L., Jin, Z.T., Shi, X. 2011. Antibacterial activity and mechanism of action of zinc oxide nanoparticles against Campylobacter jejuni. Applied and Environmental Microbiology. 77:2325-2331.
Antimicrobial activity of spherical silver nanoparticles prepared using a biocompatible macromolecular capping agent: evidence for induction of a greatly prolonged bacterial lag phase -
Irwin, P.L., Martin, J., Nguyen, L.T., He, Y., Gehring, A.G., Chen, C. 2010. Antimicrobial activity of spherical silver nanoparticles prepared using a biocompatible macromolecular capping agent: evidence for induction of a greatly prolonged bacterial lag phase. Journal of Nanobiotechnology (Biomed Central Open Access). 34:1-12.
A POPULATION STUDY OF E. COLI DOUBLING TIMES: EVIDENCE OF AN ANOMALOUS BIMODAL DISTRIBUTION BELOW A THRESHOLD CELL CONCENTRATION -
Irwin, P.L., Paoli, G., Chen, C., Nguyen, L.T. 2010. A POPULATION STUDY OF E. COLI DOUBLING TIMES: EVIDENCE OF AN ANOMALOUS BIMODAL DISTRIBUTION BELOW A THRESHOLD CELL CONCENTRATION. BMC Microbiology. 10:207.
The relationship between purely stochastic sampling error and the number of technical replicates used to estimate concentration at an extreme dilution -
Irwin, P.L., Nguyen, L.T., Chen, C. 2010. The relationship between purely stochastic sampling error and the number of technical replicates used to estimate concentration at an extreme dilution. Analytical and Bioanalytical Chemistry. DOI:10.1007/s00216-010-3967-2
Pathogen detection in produce using applications of immunomagnetic beads and biosensors -
Tu, S., Uknalis, J., Gehring, A.G., Irwin, P.L. 2009. Pathogen detection in produce using applications of immunomagnetic beads and biosensors. In: Fan, X., Niemira, B.A.. Microbial Safety of Fresh Produce. Wiley-Blackwell: IFT Press. p. 331-348.
Binding of Nontarget Organisms to Anti-Salmonella and Anti-E. coli O157 Immunomagnetic Beads: Most Probable Composition of Background Eubacteria -
Irwin, P.L., Nguyen, L., Chen, C., Paoli, G. 2008. Binding of Nontarget Organisms to Anti-Salmonella and Anti-E. coli O157 Immunomagnetic Beads: Most Probable Composition of Background Eubacteria. Analytical and Bioanalytical Chemistry. 391:525-536.
Binding of non-target microorganisms from food washes to anti-Salmonella and anti-E. coli O157 immuno-magnetic beads: minimizing the errors of random sampling in extreme dilute systems -
Irwin, P.L., Nguyen, L.T., Chen, C. 2008. Binding of non-target microorganisms from food washes to anti-Salmonella and anti-E. coli O157 immuno-magnetic beads: minimizing the errors of random sampling in extreme dilute systems. Analytical and Bioanalytical Chemistry. 391:515-524.
ENZYME-LINKED IMMUNOMAGNETIC CHEMILUMINESCENCE INCORPORATING ANTI-H7 AND ANTI-0157 ANTIBODIES FOR THE DETECTION OF ESCHERICHIA COLI 0157:H7 -
Gehring, A.G., Irwin, P.L., Reed, S.A., Tu, S. 2006. Enzyme-linked immunomagnetic chemiluminescence incorporating anti-h7 and anti-0157 antibodies for the detection of escherichia coli 0157:h7. Journal of Rapid Methods and Automation in Microbiology. 14:349-361.
DETECTION AND POTENTIAL IDENTIFICATION OF ESCHERICHIA COLI 0157:H7 USING A MICROPARTICLE-BASED CHEMILUMINESCENT IMMUNOASSAY -
Gehring, A.G., Albin, D.M., Irwin, P.L., Tu, S. 2006. Detection and potential identification of escherichia coli 0157:H7 using a microparticle-based chemiluminescent immunoassay. Biosensors p. 101.
COMPARISON OF ENZYME-LINKED IMMUNOMAGNETIC CHEMILUMINESCENT WITH USF DAS BAM METHOD FOR THE DETECTION OF ESCHERICHIA COLI O157:H7 -
Gehring, A.G., Albin, D.M., Irwin, P.L., Reed, S.A., Tu, S. 2006. Comparison of enzyme-linked immunomagnetic chemiluminescent with usf das bam method for the detection of escherichia coli o157:h7. Journal of Microbiological Methods. 67:527-533.
OXYGEN CONSUMPTION RATE OF CAMPYLOBACTER JEJUNI DURING GROWTH AND SURVIVAL UNDER VARIOUS OXYGEN LEVELS -
Chen, C., Paoli, G., Irwin, P.L. 2006. Oxygen consumption rate of campylobacter jejuni during growth and survival under various oxygen levels. {Abstract}. International Association of Food Protection. P4-59.
LUMINESCENT METHODS TO DETECT VIABLE AND TOTAL TARGETED ESCHERICHIA COLI 0157:H7 SPIKED IN GROUND BEEF -
Tu, S., Uknalis, J., Yamashoji, S., Gehring, A.G., Irwin, P.L. 2005. Luminescent methods to detect viable and total targeted escherichia coli 0157:h7 spiked in ground beef. Journal of Rapid Methods and Automation in Microbiology. 2005. 13. pp. 57-70.
BIOENERGETIC CONFIRMATION OF VIABLE PATHOGENS IN FOODS BY ATP-BIOLUMINESCENCE -
Tu, S., Gehring, A.G., Irwin, P.L. 2005. Bioenergetic confirmation of viable pathogens in foods by atp-bioluminescence. Luminescence. p. 445-448.
DETECTION OF VIABLE BACTERIA CELLS BY BIOLUMINESCENCE: A BIOENERGETIC APPROACH -
Tu, S., Wijey, C., Paoli, G., Gehring, A.G., Irwin, P.L. 2004. Detection of viable bacteria cells by bioluminescence: a bioenergetic approach. Journal of Rapid Methods and Automation in Microbiology. V.12, #3, p. 207-219.
ENZYME-LINKED IMMUNOMAGNETIC CHEMILUMINESCENT DETECTION OF ESCHERICHIA COLI 0157:H7 -
Gehring, A.G., Irwin, P.L., Reed, S.A., Tu, S., Andreotti, P.E., Akhavan-Tafti, H., Handley, R.S. 2004. Enzyme-linked immunomagnetic chemiluminescent detection of escherichia coli 0157:h7. Journal of Immunological Methods 293. 2004. p. 97-106.
DYNAMIC FACTORS CONTROLLING THE PATHOGEN CAPTURE BY IMMUNO MAGNETIC BEADS -
Tu, S., Uknalis, J., Gore, M., Irwin, P.L. 2004. Dynamic factors controlling the pathogen capture by immuno magnetic beads. American Chemical Society National Meeting.
THE EFFECT OF CAMPYLOBACTER JEJUNI POPULATION DENSITY ON SURVIVAL AND AI-2 PRODUCTION AT VARYING OXYGEN LEVELS -
Chen, C., Irwin, P.L. 2004. The effect of Campylobacter jejuni population density on survival and AI-2 production at varying oxygen levels. International Association for Food Protection Program and Abstract Book. P132. pg. 95.
BLOCKING NON-SPECIFIC ADSORPTION OF NATIVE FOOD-BORNE MICROORGANISMS BY IMMUNO-MAGNETIC BEADS WITH IOTA-CARRAGEENAN -
IRWIN, P.L., GEHRING, A.G., TU, S., CHEN, C. BLOCKING NON-SPECIFIC ADSORPTION OF NATIVE FOOD-BORNE MICROORGANISMS BY IMMUNO-MAGNETIC BEADS WITH IOTA-CARRAGEENAN. CARBOHYDRATE RESEARCH. 2004. V. 339(3) pg. 613-621
IMMUNO-MAGNETIC BEAD MASS TRANSPORT II: CAPTURE EFFICIENCY AT HIGH TARGET CELL DENSITIES IN PHOSPHATE-BUFFERED SALINE -
Irwin, P.L., Damert, W.C., Tu, S. 2004. Immuno-magnetic bead mass transport ii: capture efficiency at high target cell densities in phosphate-buffered saline. Applied and Environmental Microbiology.
RAPID DETECTION OF OUTBREAK ESCHERICHIA COLI 0157 AND SALMONELLA IN ALFALFA SPROUTS BY IMMUNOMAGNETIC CAPTURE AND TIME-RESOLVED FLOURESCENCE -
TU, S., FETT, W.F., GEHRING, A.G., IRWIN, P.L. RAPID DETECTION OF OUTBREAK ESCHERICHIA COLI 0157 AND SALMONELLA IN ALFALFA SPROUTS BY IMMUNOMAGNETIC CAPTURE AND TIME-RESOLVED FLOURESCENCE. MEETING ABSTRACT. 2003.
APPLICATIONS OF IMMUNOMAGNETIC CAPTURE AND TIME-RESOLVED FLUORESCENCE TO DETECT OUTBREAK ESCHERICHIA COLI 0157 AND SALMONELLA IN ALFALFA SPROUTS -
TU, S., FETT, W.F., GEHRING, A.G., IRWIN, P.L. APPLICATIONS OF IMMUNOMAGNETIC CAPTURE AND TIME-RESOLVED FLUORESCENCE TO DETECT OUTBREAK ESCHERICHIA COLI 0157 AND SALMONELLA IN ALFALFA SPROUTS. Proceedings of SPIE. 2003. V. 5271.
KINETIC FACTORS AFFECTING THE BACTERIAL CAPTURE EFFICIENCY OF IMMUNO BEADS: A COMPARISON BETWEEN BEADS WITH DIFFERENT SIZE AND DENSITY -
TU, S., IRWIN, P.L., GEHRING, A.G. KINETIC FACTORS AFFECTING THE BACTERIAL CAPTURE EFFICIENCY OF IMMUNO BEADS: A COMPARISON BETWEEN BEADS WITH DIFFERENT SIZE AND DENSITY. MEETING ABSTRACT. 2003.
A 6X6 DROP PLATE METHOD FOR SIMULTANEOUS COLONY COUNTING AND MPN ENUMERATION OF CAMPYLOBACTER JEJUNI, LISTERIA MONOCYTOGENES, AND ESCHERICHIA COLI -
CHEN, C., NACE JR, G.W., IRWIN, P.L. A 6X6 DROP PLATE METHOD FOR SIMULTANEOUS COLONY COUNTING AND MPN ENUMERATION OF CAMPYLOBACTER JEJUNI, LISTERIA MONOCYTOGENES, AND ESCHERICHIA COLI. JOURNAL OF MICROBIOLOGICAL METHODS. 2003.
FACTORS AFFECTING THE BACTERIAL CAPTURE EFFICIENCY OF IMMUNO BEADS: A COMPARISON BETWEEN BEADS WITH DIFFERENT SIZE AND DENSITY -
TU, S., UKNALIS, J., GORE, M., IRWIN, P.L., FEDER, I.E. FACTORS AFFECTING THE BACTERIAL CAPTURE EFFICIENCY OF IMMUNO BEADS: A COMPARISON BETWEEN BEADS WITH DIFFERENT SIZE AND DENSITY. JOURNAL OF RAPID METHODS AND AUTOMATION IN MICROBIOLOGY 11: 35-46. 2003.
SIMULTANEOUS DETECTION OF ESCHERICHIA COLI 0157:H7 AND SALMONELLA TYPHIMURIUM IN FOODS USING IMMUNOMAGNETIC CAPTURE AND LANTHANIDE TIME-RESOLVED FLUORESCENCE -
TU, S., GOLDEN, M., FEDER, I.E., IRWIN, P.L. SIMULTANEOUS DETECTION OF ESCHERICHIA COLI 0157:H7 AND SALMONELLA TYPHIMURIUM IN FOODS USING IMMUNOMAGNETIC CAPTURE AND LANTHANIDE TIME-RESOLVED FLUORESCENCE. MEETING ABSTRACT. 2003.
RAPID DETECTION OF OUTBREAK ESCHERICHIA COLI 0157 ADN SALMONELLA IN ALFALFA SPROUTS BY IMMUNOMAGNETIC CAPTURE AND TIME-RESOLVED FLUORESCENCE -
TU, S., GOLDEN, M., FETT, W.F., GEHRING, A.G., IRWIN, P.L. RAPID DETECTION OF OUTBREAK ESCHERICHIA COLI 0157 ADN SALMONELLA IN ALFALFA SPROUTS BY IMMUNOMAGNETIC CAPTURE AND TIME-RESOLVED FLUORESCENCE. JOURNAL OF FOOD SAFETY.
ENZYME-LINKED IMMUNOMAGNETIC CHEMILUMINESCENT DETECTION OF ESCHERICHIA COLI 0157:H7 -
GEHRING, A.G., IRWIN, P.L., REED, S.A., TU, S. ENZYME-LINKED IMMUNOMAGNETIC CHEMILUMINESCENT DETECTION OF ESCHERICHIA COLI 0157:H7. PITTSBURGH CONFERENCE. 2003.
SIMULTANEOUS DETECTION OF ESCHERICHIA COLI 0157:H7 AND SALMONELLA TYPHIMURIUM IN FOODS USING IMMUNOMAGNETIC CAPTURE AND LANTHANIDE TIME-RESOLVED FLUORESCENCE -
TU, S., GOLDEN, M., IRWIN, P.L., GEHRING, A.G., FEDER, I.E. SIMULTANEOUS DETECTION OF ESCHERICHIA COLI 0157:H7 AND SALMONELLA TYPHIMURIUM IN FOODS USING IMMUNOMAGNETIC CAPTURE AND LANTHANIDE TIME-RESOLVED FLUORESCENCE. PITTSBURGH CONFERENCE. 2003.
THE CAPTURE OF ESCHERICHIA COLI 0157:H7 FOR LIGHT ADDRESSABLE POTENTIOMETRIC SENSOR (LAPS) USING TWO DIFFERENT TYPES OF MAGNETIC BEADS -
DEVELOPMENT OF BIOSENSOR PROCESSES FOR RAPID, SPECIFIC AND SENSITIVE DETECTION OF PATHOGENIC BACTERIA IN FOODS -
THE USE OF TIME-RESOLVED FLUOROIMMUNOASSAY TO SIMULTANEOUSLY DETECT ESCHERICHIA COLI 0157:H7, SALMONELLA TYPHIMURIUM AND SALMONELLA ENTERIDITISIN FOODS -
EVIDENCE FOR BUOYANCY-DEPENDENT IMB-SALMONELLA COMPLEX LOSSES DURING MAGNETIC PHASE SEPARATION -
IRWIN, P.L., FORTIS, L.L., CHAU, L.I., GEHRING, A.G., TU, S. EVIDENCE FOR BUOYANCY-DEPENDENT IMB-SALMONELLA COMPLEX LOSSES DURING MAGNETIC PHASE SEPARATION. JOURNAL OF RAPID METHODS AND AUTOMATION IN MICROBIOLOGY. 2002.
APPLICATIONS OF TIME-RESOLVED FLUOROIMMUNOASSAY TO DETECT MAGNETIC BEAD CAPTURED ESCHERICHIA COLI O157:H7 -
A SIMPLE MAXIMUM PROBABILITY RESOLUTION ALGORITHM FOR MOST PROBABLE NUMBER ANALYSIS USING MICROSOFT EXCEL -
DETECTION OF IMMUNOMAGNETICALLY CAPTURED ESCHERICHIA COLI O157:H7 BY ANTIBODY-CONJUGATED ALKALINE PHOSPHATASE -
Last Modified: 5/2/2016
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