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United States Department of Agriculture

Agricultural Research Service

Related Topics

Joseph M Bosilevac (Mick)
Meat Safety & Quality Research
Research Microbiologist

Phone: (402) 762-4225
Fax: (402) 762-4149
U.S. MEAT ANIMAL RESEARCH CENTER
SPUR 18D
CLAY CENTER, NE, 68933

Projects
PATHOGEN MITIGATION IN LIVESTOCK AND RED MEAT PRODUCTION
Appropriated (D)
  Accession Number: 420868
TECHNOLOGIES AS MULTIPLE HURDLES TO INACTIVATE SHIGA TOXIN-PRODUCING ESCHERICHIA COLI & VIRUSES DURING BEEF PROCESSING & ON NON-INTACT BEEF
Reimbursable (R)
  Accession Number: 421371

Publications (Clicking on the reprint icon Reprint Icon will take you to the publication reprint.)
Examination of predictors of Salmonella enterica contamination in cattle feedlot environments - (Abstract Only)
Harhay, D.M., Arthur, T.M., Schmidt, J.W., Wang, R., Kalchayanand, N., Bosilevac, J.M., Edrington, T.S., Loneragan, G., Wheeler, T.L. 2014. Examination of predictors of Salmonella enterica contamination in cattle feedlot environments. [Abstract] Beef Industray Safety Summit. Available: www.bifsco.org/attendees.aspx.
Prevalence and serotypes of intimin (eae) positive Shiga toxin-producing E. coli colonizing cattle arriving at fed beef and cull cow plants in the US - (Abstract Only)
Bosilevac, J.M., Luedtke, B.E., Arthur, T.M., Kalchayanand, N., Schmidt, J.W., Shackelford, S.D., Wheeler, T.L. 2014. Prevalence and serotypes of intimin (eae) positive Shiga toxin-producing E. coli colonizing cattle arriving at fed beef and cull cow plants in the U.S. [abstract] Beef Industry Safety Summit. Available: www.bifsco.org/attendees.aspx.
Effects of in-plant interventions on reduction of enterohemorrhagic Escherichia coli and background indicator microorganisms on veal calf hides - (Peer Reviewed Journal) - (09-Dec-13)
Immersion in antimicrobial solutions reduces Salmonella enterica and Shiga toxin-producing Escherichia coli on beef cheek meat - (Peer Reviewed Journal)
Schmidt, J.W., Bosilevac, J.M., Kalchayanand, N., Wang, R., Wheeler, T.L., Koohmaraie, M. 2014. Immersion in antimicrobial solutions reduces Salmonella enterica and Shiga toxin-producing Escherichia coli on beef cheek meat. Journal of Food Protection. 77(4):538-548.
Impact of sampling area and location on measurement of indicator organisms during beef carcass interventions - (Peer Reviewed Journal) - (13-Jul-13)
Biofilm formation and sanitizer resistance contributes to “High Event” meat contamination by E. coli O157:H7 - (Abstract Only)
Wang, R., Kalchayanand, N., Bosilevac, J.M., Arthur, T.M. 2013. Biofilm formation and sanitizer resistance contributes to “High Event” meat contamination by E. coli O157:H7. International Association for Food Protection Proceedings. [abstract] P1-48 Page No.48.
Ultraviolet light (UV) and UV-ozone interventions reduce shiga toxin-producing Escherichia coli (STEC) on contaminated fresh beef - (Abstract Only)
Kalchayanand, N., Bosilevac, J.M., Wheeler, T.L. 2013. Ultraviolet light (UV) and UV-ozone interventions reduce shiga toxin-producing Escherichia coli (STEC) on contaminated fresh beef. American Society for Microbiology General Meeting. Poster No. 1596 p.166.
Cross-sectional study examining Salmonella enterica carriage in subiliac lymph nodes of cull and feedlot cattle at harvest Reprint Icon - (Peer Reviewed Journal)
Gragg, S.E., Loneragan, G.H., Brashears, M.M., Arthur, T.M., Bosilevac, J.M., Kalchayanand, N., Wang, R., Schmidt, J.W., Brooks, J.C., Shackelford, S.D., Wheeler, T.L., Brown, T.R., Edrington, T.S., Brichta-Harhay, D.M. 2013. Cross-sectional study examining Salmonella enterica carriage in subiliac lymph nodes of cull and feedlot cattle at harvest. Foodborne Pathogens and Disease. 10:368-374.
Salmonella virulence, genomics and interactions with the immune system - (Proceedings)
Harhay, D.M., Smith, T.P., Harhay, G.P., Bono, J.L., Bosilevac, J.M. 2013. Salmonella virulence, genomics and interactions with the immune system. Meeting Abstract. The Beef Industry Safety Summit, March 13-15, 2013, Dallas Texas, FLASHDRIVE.
Salmonella in lymph nodes of cull and fed cattle at harvest - (Abstract Only)
Webb, H.E., Harhay, D.M., Gragg, S.E., Brashears, M.M., Nightingale, K.K., Arthur, T.M., Bosilevac, J.M., Kalchayanand, N., Schmidt, J.W., Wang, R., Lonergan, G.H. 2013. Salmonella in lymph nodes of cull and fed cattle at harvest. IN: Proceedings of The Beef Industry Summit 2013, March 13-15, 2013, Dallas Texas, 2013 FLASHDRIVE.
Comparison of non-O157 Shiga toxin-producing E. coli detection systems - (Proceedings)
Bosilevac, J.M. 2013. Comparison of non-O157 Shiga toxin-producing E. coli detection systems.Proceedings of the Beef Industry Safety Summit, March 13-15, 2013, Dallas Texas. 2013 FLASHDRIVE.
Cross-sectional study examining Salmonella enterica carriage in subiliac lymph nodes of cull and feedlot cattle at harvest Reprint Icon - (Peer Reviewed Journal)
Gragg, S.E., Loneragan, G.H., Brashears, M.M., Arthur, T.M., Bosilevac, J.M., Kalchayanand, N., Wang, R., Schmidt, J.W., Brooks, J., Shackelford, S.D., Wheeler, T.L., Brown, T.R., Edrington, T.S., Harhay, D.M. 2013. Cross-sectional study examining Salmonella enterica carriage in subiliac lymph nodes of cull and feedlot cattle at harvest. Foodborne Pathogens and Disease. 10(4):368-374.
Isolation and characterization of Clostridium difficile associated with beef cattle and commercially produced ground beef Reprint Icon - (Peer Reviewed Journal)
Kalchayanand, N., Arthur, T.M., Bosilevac, J.M., Harhay, D.M., Shackelford, S.D., Wells, J., Wheeler, T.L., Koohmaraie, M. 2013. Isolation and characterization of Clostridium difficile associated with beef cattle and commercially produced ground beef. Journal of Food Protection. 76(2):256-264.
The physiologic state of Escherichia coli O157:H7 does not affect its detection in two commercial real-time PCR-based tests Reprint Icon - (Peer Reviewed Journal)
Wang, R., Schmidt, J.W., Arthur, T.M., Bosilevac, J.M. 2013. The physiologic state of Escherichia coli O157:H7 does not affect its detection in two commercial real-time PCR-based tests. Food Microbiology. 33: 205-212.
Chromogenic agar medium for detection and isolation of Escherichia coli serogroups O26,O45,O103,O111,O121, and O145 from fresh beef and cattle feces Reprint Icon - (Peer Reviewed Journal)
Kalchayanand, N., Arthur, T.M., Bosilevac, J.M., Wells, J., Wheeler, T.L. 2013. Chromogenic agar medium for detection and isolation of Escherichia coli serogroups O26,O45,O103,O111,O121, and O145 from fresh beef and cattle feces. Journal of Food Protection. 76(2):192-199.
Detection of Escherichia coli O157:H7 and Salmonella enterica in air and droplets at three United States commercial beef processing plants Reprint Icon - (Peer Reviewed Journal)
Schmidt, J.W., Arthur, T.M., Bosilevac, J.M., Kalchayanand, N., Wheeler, T.L. 2012. Detection of Escherichia coli O157:H7 and Salmonella enterica in air and droplets at three United States commercial beef processing plants. Journal of Food Protection. 75(12):2213-2218.
Predicting the presence of non-O157 Shiga toxin-producing Escherichia coli in ground beef by using molecular tests for Shiga toxins, intimin, and O serogroups Reprint Icon - (Peer Reviewed Journal)
Bosilevac, J.M., Koohmaraie, M. 2012. Predicting the presence of non-O157 Shiga toxin-producing Escherichia coli in ground beef by using molecular tests for Shiga toxins, intimin, and O serogroups. Applied and Environmental Microbiology. 78(19):7152-7155.
Resistance of various shiga toxin-producing Escherichia coli to electrolyzed oxidizing water Reprint Icon - (Peer Reviewed Journal)
Jadeja, R., Hung, Y., Bosilevac, J.M. 2013. Resistance of various shiga toxin-producing Escherichia coli to electrolyzed oxidizing water. Food Control. 30(2):580-584.
Dual-serotype biofilm formation by shiga toxin-producing Escherichia coli 0157:H7 and 026:H11 strains - (Peer Reviewed Journal)
Wang, R., Kalchayanand, N., Schmidt, J.W., Bosilevac, J.M., Bono, J.L. 2012. Dual-Serotype Biofilm Formation by Shiga Toxin-Producing Escherichia coli 0157:H7 and 026:H11 strains. Applied and Environmental Microbiology. 78(17):6341-6344.
Detection methods and intervention strategies for shiga toxin-producing E. coli in beef - (Abstract Only)
Kalchayanand, N., Arthur, T.M., Bosilevac, J.M., Wheeler, T.L. 2012. Detection methods and intervention strategies for shiga toxin-producing E. coli in beef [abstract]. Annual Meeting of the Institute of Food Technologists. 061-01:52.
Evaluation of commonly used antimicrobial interventions for fresh beef inoculated with Shiga toxin-producing Escherichia coli serotypes O26, O45, O103, O111, O121, O145, and O157:H7 - (Peer Reviewed Journal)
Kalchayanand, N., Arthur, T.M., Bosilevac, J.M., Schmidt, J.W., Wang, R., Shackelford, S.D., Wheeler, T.L. 2012. Evaluation of commonly used antimicrobial interventions for fresh beef inoculated with Shiga toxin-producing Escherichia coli serotypes O26, O45, O103, O111, O121, O145, and O157:H7. Journal of Food Protection. 75(7):1207-1212.
Efficacy of ultraviolet light (UV) and UV-ozone as an intervention to reduce pathogens-contaminated fresh beef - (Abstract Only)
Kalchayanand, N., Bosilevac, J.M., Wheeler, T.L. 2012. Efficacy of ultraviolet light (UV) and UV-ozone as an intervention to reduce pathogens-contaminated fresh beef.[Abstract] Annual Meeting of the Institute of Food Technologists.June 25-28, 2012, Las Vegas, Nevada. Abstract 035-71:25.
Prevalence and characterization of non-O157 Shiga toxin-producing Escherichia coli isolated from feedlot and cull dairy and beef herd cattle - (Abstract Only) - (31-Jan-12)
Prevalence, enumeration, serotypes, and antimicrobial resistance phenotypes of Salmonella enterica isolates from carcasses at two large United States pork processing plants - (Peer Reviewed Journal)
Schmidt, J.W., Harhay, D.M., Kalchayanand, N., Bosilevac, J.M., Shackelford, S.D., Wheeler, T.L., Koohmaraie, M. 2012. Prevalence, enumeration, serotypes, and antimicrobial resistance phenotypes of Salmonella enterica isolates from carcasses at two large United States pork processing plants. Applied and Environmental Microbiology. 78(8):2716-2726.
Microbiological analysis of bovine lymph nodes for the detection of Salmonella enterica - (Peer Reviewed Journal)
Harhay, D.M., Arthur, T.M., Bosilevac, J.M., Kalchayanand, N., Schmidt, J.W., Wang, R., Shackelford, S.D., Loneragan, G.H., Wheeler, T.L. 2012. Microbiological analysis of bovine lymph nodes for the detection of Salmonella enterica. Journal of Food Protection. 75(5):854-858.
Salmonella in lymph nodes of cattle presented for harvest - (Abstract Only)
Gragg, S.E., Loneragan, G.H., Brashears, M.M., Arthur, T.M., Bosilevac, J.M., Kalchayanand, N., Wang, R., Schmidt, J.W., Brooks, J.C., Shackelford, S.D., Wheeler, T.L., Brown, T.R., Brichta-Harhay, D.M. 2011. Salmonella in lymph nodes of cattle presented for harvest [abstract]. Proceeding of the 2011 Conference of Research Workers in Animal Diseases, December 4-6, 2011, Chicago, Illinois. p. 149.
Impact of reducing the level of wet distillers grains fed to cattle prior to harvest on prevalence and levels of Escherichia coli O157:H7 in feces and on hides - (Peer Reviewed Journal)
Wells, J., Shackelford, S.D., Berry, E.D., Kalchayanand, N., Bosilevac, J.M., Wheeler, T.L. 2011. Impact of reducing the level of wet distillers grains fed to cattle prior to harvest on prevalence and levels of Escherichia coli O157:H7 in feces and on hides. Journal of Food Protection. 74(10):1611-1617.
Non-O157 Shiga toxin-producing Escherichia coli: prevalence associated with meat animals and controlling interventions - (Proceedings)
Kalchayanand, N., Arthur, T.M., Bosilevac, J.M., Wheeler, T.L. 2012. Non-O157 Shiga toxin-producing Escherichia coli: prevalence associated with meat animals and controlling interventions. Proceedings of American Meat Science Association 64th Annual Reciprocal Meat Conference, June 19-22, 2011, Kansas State University, Manhattan Kansas. p.1-9.
Reduction in dietary wet distillers grains alters Escherichia coli 0157:H7 in feces of cattle - (Abstract Only)
Wells, J., Shackelford, S.D., Berry, E.D., Kalchayanand, N., Bosilevac, J.M., Wheeler, T.L. 2011. Reduction in dietary wet distillers grains alters Escherichia coli 0157:H7 in feces of cattle. In: Proceedings of the 2011 Congress on Gastrointestinal Function, April 18-20, 2011, Chicago, Illinois.
Survival of Escherichia coli O157:H7 on cattle hides - (Peer Reviewed Journal)
Arthur, T.M., Nou, X., Kalchayanand, N., Bosilevac, J.M., Wheeler, T.L., Koohmaraie, M. 2011. Survival of Escherichia coli O157:H7 on cattle hides. Applied and Environmental Microbiology. 77(9):3002-3008.
Sensitivity of Mycobacterium bovis to common beef processing interventions - (Abstract Only)
Bosilevac, J.M., Iwen, P.C. 2011. Sensitivity of Mycobacterium bovis to common beef processing interventions [abstract]. International Association for Food Protection, Milwaukee, WI. July 31 - August 3, 2011. Poster Abstract P2-42, p. 65.
Salmonella in lymph nodes of cattle presented for harvest - (Abstract Only)
Gragg, S.E., Loneragan, G.H., Brichta-Harhay, D.M., Arthur, T.M., Bosilevac, J.M., Kalchayanand, N., Wang, R., Schmidt, J.W., Brooks, J.C., Shackelford, S.D., Wheeler, T.L., Brown, T.R., Brashears, M.M. 2011. Salmonella in lymph nodes of cattle presented for harvest [abstract]. International Association for Food Protection. p. 19. Abstract No. P1-62.
Sensitivity of Mycobacterium bovis to common beef processing interventions - (Abstract Only)
Bosilevac, J.M., Iwen, P.C. 2011. Sensitivity of Mycobacterium bovis to common beef processing interventions [abstract]. 2011 Beef Safety Summit, Dallas, TX. March 2-4, 2011. 2011 Beef Industry Safety Summit Research Update. Abstract No. 8.
The effect of sample size and matrix on the ability of rapid methods to detect E. coli O157:H7 - (Abstract Only)
Bosilevac, J.M., Schmidt, J.W., Kalchayanand, N., Wheeler, T.L., Koohmaraie, M. 2011. The effect of sample size and matrix on the ability of rapid methods to detect E. coli O157:H7 [abstract]. 2011 Beef Industry Safety Summit, Dallas, TX. March 2-4, 2011. 2011 Beef Industry Safety Summit Research Update. Abstract No. 1.
Towards an understanding of how Salmonella potentially evades beef carcass processing interventions - (Abstract Only)
Brichta-Harhay, D.M., Gragg, S.E., Smith, T.P.L., Arthur, T.M., Bosilevac, J.M., Kalchayanand, N., Schmidt, J.W., Wang, R., Shackelford, S.D., Wheeler, T.L., Brashears, M.M., Brooks, J.C., Brown, T.R., Harhay, G.P., Loneragan, G.H. 2011. Towards an understanding of how Salmonella potentially evades beef carcass processing interventions [abstract]. 2011 Beef Industry Safety Summit. Abstract No. 2.
Prevalence and characterization of non-O157 Shiga toxin producing Escherichia coli isolates from commercial ground beef in the United States - (Peer Reviewed Journal)
Bosilevac, J.M., Koohmaraie, M. 2011. Prevalence and characterization of non-O157 Shiga toxin producing Escherichia coli isolates from commercial ground beef in the United States. Applied and Environmental Microbiology. 77(6):2103-2112.
Diversity of multidrug-resistant Salmonella enterica strains associated with cattle at harvest in the United States - (Peer Reviewed Journal)
Harhay, D.M., Arthur, T.M., Bosilevac, J.M., Kalchayanand, N., Shackelford, S.D., Wheeler, T.L., Koohmaraie, M. 2011. Diversity of multidrug-resistant Salmonella enterica strains associated with cattle at harvest in the United States. Applied and Environmental Microbiology. 77(5):1783-1796.
Inoculation of beef with low concentrations of Escherichia coli O157:H7 and examination of factors that interfere with its detection by culture isolation and rapid methods - (Peer Reviewed Journal)
Bosilevac, J.M., Kalchayanand, N., Schmidt, J.W., Shackelford, S.D., Wheeler, T.L., Koohmaraie, M. 2010. Inoculation of beef with low concentrations of Escherichia coli O157:H7 and examination of factors that interfere with its detection by culture isolation and rapid methods. Journal of Food Protection. 73:2180-2188.
Diversity of Multi-drug Resistant Salmonella enterica Associated with Cull Cattle at Harvest in the United States - (Abstract Only)
Harhay, D.M., Poole, T.L., Durso, L.M., Arthur, T.M., Bosilevac, J.M., Kalchayanand, N., Shackelford, S.D., Wheeler, T.L., Koohmaraie, M. 2010. Diversity of Multi-drug Resistant Salmonella enterica Associated with Cull Cattle at Harvest in the United States [abstract]. American Society for Microbiology. p. 25, Presentation No. S4:1 and p. 111, Poster No. B176.
Super Shedding of Escherichia coli O157:H7 by Cattle and the Impact on Beef Carcass Contamination - (Proceedings)
Arthur, T.M., Harhay, D.M., Bosilevac, J.M., Kalchayanand, N., Shackelford, S.D., Wheeler, T.L., Koohmaraie, M. 2010. Super Shedding of Escherichia coli O157:H7 by Cattle and the Impact on Beef Carcass Contamination. Meat Science. 86:32-37. Special Issue: 56th International Congress of Meat Science and Technology (56th ICoMST) August 15-20, 2010, Jeju, Korea. (Proceedings) Available: http://dx.doi.org/10.1016/j.meatsci.2010.04.019
Evaluation of a Direct-Fed Microbial Product Effect on the Prevalence and Load of Escherichia coli O157:H7 in Feedlot Cattle - (Peer Reviewed Journal)
Arthur, T.M., Bosilevac, J.M., Kalchayanand, N., Wells, J., Shackelford, S.D., Wheeler, T.L., Koohmarie, M. 2010. Evaluation of a Direct-Fed Microbial Product Effect on the Prevalence and Load of Escherichia coli O157:H7 in Feedlot Cattle. Journal of Food Protection. 73(2):366-371.
Longitudinal Study of Escherichia coli O157:H7 in a Beef Cattle Feedlot and Role of High-Level Shedders in Hide Contamination - (Peer Reviewed Journal)
Arthur, T.M., Keen, J.E., Bosilevac, J.M., Brichta-Harhay, D.M., Kalchayanand, N., Shackelford, S.D., Wheeler, T.L., Nou, X., Koohmaraie, M. 2009. Longitudinal Study of Escherichia coli O157:H7 in a Beef Cattle Feedlot and Role of High-Level Shedders in Hide Contamination. Applied and Environmental Microbiology. 75(20):6515-6523.
Prevalence and Level of Escherichia coli O157:H7 in Feces and on Hides of Feedlot Steers Fed Diets With or Without Wet Distillers Grains with Solubles - (Peer Reviewed Journal)
Wells, J., Shackelford, S.D., Berry, E.D., Kalchayanand, N., Guerini, M.N., Varel, V.H., Arthur, T.M., Bosilevac, J.M., Freetly, H.C., Wheeler, T.L., Ferrell, C.L., Koohmaraie, M. 2009. Prevalence and Level of Escherichia coli O157:H7 in Feces and on Hides of Feedlot Steers Fed Diets With or Without Wet Distillers Grains with Solubles. Journal of Food Protection. 72(8):1624-1633.
Non-O157 Shiga toxin Producing Escherichia coli in United States Beef Production - (Abstract Only)
Bosilevac, J.M., Koohmaraie, M. 2009. Non-O157 Shiga toxin Producing Escherichia coli in United States Beef Production [abstract]. 7th International Symposium on Shiga Toxin (Verocytotoxin)-Producing Escherichia coli Infections (VTEC2009) Paper No. P03.2.2. p 71.
Comparison of Colony Hybridization to Phenotype Screening on Washed Sheep's Blood Agar for the Isolation of Shiga toxin Producing Escherichia coli from Complex Matrixes - (Abstract Only)
Bosilevac, J.M., Koohmaraie, M. 2009. Comparison of Colony Hybridization to Phenotype Screening on Washed Sheep's Blood Agar for the Isolation of Shiga toxin Producing Escherichia coli from Complex Matrixes [abstract]. 7th International Symposium on Shiga Toxin (Verocytotoxin)-Producing Escherichia coli Infections (VTEC2009) Paper No. P02.5.3. p. 106.
Longitudinal Study of Escherichia coli O157:H7 in a Beef Cattle Feedlot and the Role of High Shedders in Hide Contamination - (Abstract Only)
Arthur, T.M., Keen, J.E., Bosilevac, J.M., Brichta-Harhay, D.M., Kalchayanand, N., Shackelford, S.D., Wheeler, T.L., Nou, X., Koohmaraie, M. 2009. Longitudinal Study of Escherichia coli O157:H7 in a Beef Cattle Feedlot and the Role of High Shedders in Hide Contamination [abstract]. 7th International Symposium on Shiga Toxin (Verocytotoxin)-Producing Escherichia coli Infections (VTEC2009) Paper No. 195.
Prevalence of Mycobacterium avium subsp. paratuberculosis in Ileocecal Lymph Nodes and on Hides and Carcasses from Cull Cows and Fed Cattle at Commercial Beef Processing Plants in the United States - (Peer Reviewed Journal)
Wells, J., Bosilevac, J.M., Kalchayanand, N., Arthur, T.M., Shackelford, S.D., Wheeler, T.L., Koohmaraie, M. 2009. Prevalence of Mycobacterium avium subsp. paratuberculosis in Ileocecal Lymph Nodes and on Hides and Carcasses from Cull Cows and Fed Cattle at Commercial Beef Processing Plants in the United States. Journal of Food Protection 72(7):1457-1462.
Prevalence and Level of Escherichia coli O157:H7 in Feces and On Hides of Feedlot Steers Fed Diets With or Without Wet Distillers Grains With Solubles Reprint Icon - (Abstract Only)
Wells, J., Shackelford, S.D., Berry, E.D., Kalchayanand, N., Varel, V.H., Arthur, T.M., Bosilevac, J.M., Freetly, H.C., Wheeler, T.L., Ferrell, C.L., Koohmaraie, M. 2009. Prevalence and Level of Escherichia coli O157:H7 in Feces and On Hides of Feedlot Steers Fed Diets With or Without Wet Distillers Grains With Solubles [abstract]. Microbial Ecology 57(3):583-584.
Prevalence of Clostridium difficile from Commercial Beef Processing Plants in the United States - (Abstract Only)
Kalchayanand, N., Arthur, T.M., Bosilevac, J.M., Harhay, D.M., Shackelford, S.D., Wells, J., Wheeler, T.L., Koohmaraie, M. 2009. Prevalence of Clostridium difficile from Commercial Beef Processing Plants in the United States [abstract]. Institute of Food Technologists (IFT), Division Food Microbiology, Session 026-15, p. 82.
Prevalence and Characterization of Salmonellae in Commercial Ground Beef in the United States - (Peer Reviewed Journal)
Bosilevac, J.M., Guerini, M.N., Kalchayanand, N., Koohmaraie, M. 2009. Prevalence and Characterization of Salmonellae in Commercial Ground Beef in the United States. Applied and Environmental Microbiology. 75(7):1892-1900.
Prevalence Rates of Escherichia coli O157:H7 and Salmonella at Different Sampling Sites on Cattle Hides at a Feedlot and Processing Plant - (Peer Reviewed Journal)
Kalchayanand, N., Brichta-Harhay, D.M., Arthur, T.M., Bosilevac, J.M., Guerini, M.N., Wheeler, T.L., Shackelford, S.D., Koohmaraie, M. 2009. Prevalence Rates of Escherichia coli O157:H7 and Salmonella at Different Sampling Sites on Cattle Hides at a Feedlot and Processing Plant. Journal of Food Protection. 72(6):1267-1271.
Escherichia coli O157:H7 and Salmonella in Small Processing Plants and the Effect of Feeding Wet Distillers Grains with Solubles on E. coli O157:H7 - (Abstract Only)
Bosilevac, J.M., Wells, J., Arthur, T.M., Kalchayanand, N., Shackelford, S.D., Berry, E.D., Bono, J.L., Harhay, D.M., Wheeler, T.L., Koohmaraie, M. 2009. Escherichia coli O157:H7 and Salmonella in Small Processing Plants and the Effect of Feeding Wet Distillers Grains with Solubles on E. coli O157:H7 [abstract]. NCBA Beef Industry Safety Summit, San Diego, CA, March 4-6, 2009.
Characterization of Multidrug-resistant Salmonella Isolated from Cull and Fed Cattle at Slaughter in the United States - (Abstract Only)
Harhay, D.M., Arthur, T.M., Bosilevac, J.M., Kalchayanand, N., Shackelford, S.D., Wheeler, T.L., Koohmaraie, M. 2009. Characterization of Multidrug-resistant Salmonella Isolated from Cull and Fed Cattle at Slaughter in the United States [abstract]. NCBA Beef Industry Safety Summit, San Diego, CA, March 4-6, 2009.
Prevalence and Enumeration of Escherichia coli O157:H7 and Salmonella in U.S. Abattoirs That Process Fewer Than 1,000 Head of Cattle Per Day - (Peer Reviewed Journal)
Bosilevac, J.M., Arthur, T.M., Bono, J.L., Brichta-Harhay, D.M., Kalchayanand, N., King, D.A., Shackelford, S.D., Wheeler, T.L., Koohmaraie, M. 2009. Prevalence and Enumeration of Escherichia coli O157:H7 and Salmonella in U.S. Abattoirs That Process Fewer Than 1,000 Head of Cattle Per Day. Journal of Food Protection. 72(6):1272-1278.
Effectiveness of 1,3-Dibromo-5,5 dimethylhydantoin on reduction of Escherichia coli O157:H7- and Salmonella-inoculated fresh meat - (Peer Reviewed Journal)
Kalchayanand, N., Arthur, T.M., Bosilevac, J.M., Brichta-Harhay, D.M., Guerini, M.N., Shackelford, S.D., Wheeler, T.L., Koohmaraie, M. 2009. Effectiveness of 1,3-Dibromo-5,5 dimethylhydantoin on reduction of Escherichia coli O157:H7- and Salmonella-inoculated fresh meat. Journal of Food Protection 72(1):151-156.
Increased Detection of Listeria Species and Listeria Monocytogenes in Raw Beef, Using the Assurance GDS Molecular Detection System with Culture Isolation - (Peer Reviewed Journal)
Bosilevac, J.M., Guerini, M.N., Koohmaraie, M. 2009. Increased Detection of Listeria Species and Listeria Monocytogenes in Raw Beef, Using the Assurance GDS Molecular Detection System with Culture Isolation. Journal of Food Protection. 72(3):674-679.
Salmonella and E. coli O157:H7 contamination on hides and carcasses of cull cattle presented for slaughter in the U.S.: an evaluation of prevalence and bacterial loads by immunomagnetic separation and direct plating methods - (Peer Reviewed Journal)
Harhay, D.M., Guerini, M.N., Arthur, T.M., Bosilevac, J.M., Kalchayanand, N., Shackelford, S.D., Wheeler, T.L., Koohmaraie, M. Salmonella and Escherichia coli O157:H7 contamination on hides and carcasses of cull cattle presented for slaughter in the United States: an evaluation of prevalence and bacterial loads by immunomagnetic separation and direct plating methods. 2008. Applied and Environmental Microbiology 74(20):6289-6297.
Comparison of effects of antimicrobial interventions on multidrug-resistant Salmonella, susceptible Salmonella, and Escherichia coli O157:H7 - (Peer Reviewed Journal)
Arthur, T.M., Kalchayanand, N., Bosilevac, J.M., Harhay, D.M., Shackelford, S.D., Bono, J.L., Wheeler, T.L., Koohmaraie, M. 2008. Comparison of effects of antimicrobial interventions on multidrug-resistant Salmonella, susceptible Salmonella, and Escherichia coli O157:H7. Journal of Food Protection 71(11):2177-2181.
Source tracking of Escherichia coli O157:H7 and Salmonella contamination in the lairage environment at commercial U.S. beef processing plants and identification of an effective intervention - (Peer Reviewed Journal)
Arthur, T.M., Bosilevac, J.M., Harhay, D.M., Kalchayanand, N., King, D.A., Shackelford, S.D., Wheeler, T.L., Koohmaraie, M. 2008. Source tracking of Escherichia coli O157:H7 and Salmonella contamination in the lairage environment at commercial U.S. beef processing plants and identification of an effective intervention. Journal of Food Protection. 71(9):1752-1760.
Effects of using reduced volumes of nonselective enrichment medium in methods for the detection of Escherichia coli O157:H7 from raw beef - (Review Article)
Bosilevac, J.M., Koohmaraie, M. 2008. Effects of using reduced volumes of nonselective enrichment medium in methods for the detection of Escherichia coli O157:H7 from raw beef. Journal of Food Protection 71(9):1768-1773.
Prevalence and Characterization of Salmonella in Bovine Lymph Nodes Potentially Destined for Use in Ground Beef - (Peer Reviewed Journal)
Arthur, T.M., Harhay, D.M., Bosilevac, J.M., Guerini, M.N., Kalchayanand, N., Wells, J., Shackelford, S.D., Wheeler, T.L., Koohmaraie, M. 2008. Prevalence and characterization of Salmonella in bovine lymph nodes potentially destined for use in ground beef. Journal of Food Protection 71(8):1685-1688.
Evaluation of various antimicrobial interventions for the reduction of Escherichia coli O157:H7 on bovine heads during processing - (Peer Reviewed Journal)
Kalchayanand, N., Arthur, T.M., Bosilevac, J.M., Brichta-Harhay, D.M., Guerini, M.N., Wheeler, T.L., Koohmaraie, M. 2008. Evaluation of various antimicrobial interventions for the reduction of Escherichia coli O157:H7 on bovine heads during processing. Journal of Food Protection 71(3):621-624.
Listeria prevalence and L. monocytogenes serovar diversity at cull cow/bull processing plants in the United States - (Peer Reviewed Journal)
Guerini, M.N., Harhay, D.M., Shackelford, S.D., Arthur, T.M., Bosilevac, J.M., Kalchayanand, N., Wheeler, T.L., Koohmaraie, M. 2007. Listeria prevalence and Listeria monocytogenes serovar diversity at cull cow and bull processing plants in the United States. Journal of Food Protection. 70(11):2578-2582.
Comparison of the molecular genotypes of Escherichia coli O157:H7 from the hides of beef cattle in different regions of North America - (Peer Reviewed Journal)
Arthur, T.M., Bosilevac, J.M., Nou, X., Shackelford, S.D., Wheeler, T.L., Koohmaraie, M. 2007. Comparison of the molecular genotypes of Escherichia coli O157:H7 from the hides of beef cattle in different regions of North America. Journal of Food Protection. 70(7):1622-1626.
Microbiological characterization of lamb carcasses at commercial processing plants in the United States - (Peer Reviewed Journal)
Kalchayanand, N., Arthur, T.M., Bosilevac, J.M., Harhay, D.M., Guerini, M.N., Shackelford, S.D., Wheeler, T.L., Koohmaraie, M. 2007. Microbiological characterization of lamb carcasses at commercial processing plants in the United States. Journal of Food Protection 70(8):1811-1819.
Enumeration of Salmonella and Escherichia coli O157:H7 in Ground Beef, Cattle Carcass, Hide and Fecal Samples Using Direct Plating Methods - (Peer Reviewed Journal)
Harhay, D.M., Arthur, T.M., Bosilevac, J.M., Guerini, M.N., Kalchayanand, N., Koohmaraie, M. 2007. Enumeration of Salmonella and Escherichia coli O157:H7 in ground beef, cattle carcass, hide and faecal samples using direct plating methods. Journal of Applied Microbiology 103(5):1657-1668.
Effects of a Minimal Hide Wash Cabinet on the Levels and Prevalence of Escherichia coli O157:H7 and Salmonella on the Hides of Beef Cattle at Slaughter - (Peer Reviewed Journal)
Arthur, T.M., Bosilevac, J.M., Brichta-Harhay, D.M., Kalchayanand, N., Shackelford, S.D., Wheeler, T.L., Koohmaraie, M. 2007. Effects of a Minimal Hide Wash Cabinet on the Levels and Prevalence of Escherichia coli O157:H7 and Salmonella on the Hides of Beef Cattle at Slaughter. Journal of Food Protection 70:1076-1079.
Transportation and lairage environment effects on prevalence, numbers, and diversity of Escherichia coli O157:H7 on hides and carcasses of beef cattle at processing - (Peer Reviewed Journal)
Arthur, T.M., Bosilevac, J.M., Harhay, D.M., Guerini, M.N., Kalchayanand, N., Shackelford, S.D., Wheeler, T.L., Koohmaraie, M. 2007. Transportation and lairage environment effects on prevalence, numbers, and diversity of Escherichia coli O157:H7 on hides and carcasses of beef cattle at processing. Journal of Food Protection 70(2):280-286.
Microbiological Characterization of Imported and Domestic Boneless Beef Trim Used for Ground Beef - (Peer Reviewed Journal)
Bosilevac, J.M., Guerini, M.N., Harhay, D.M., Arthur, T.M., Koohmaraie, M. 2007. Microbiological characterization of imported and domestic boneless beef trim used for ground beef. Journal of Food Protection 70(2):440-449.
Rapid Enrichment Strategy for Isolation of Listeria from Bovine Hide, Carcass, and Meat Samples - (Peer Reviewed Journal)
Guerini, M.N., Bosilevac, J.M., Koohmaraie, M. 2007. Rapid enrichment strategy for isolation of Listeria from bovine hide, carcass, and meat samples. Journal of Food Protection 70(1):53-57.
Improvement of Immunomagnetic Separation for Escherichia Coli O157:h7 Detection by the Pickpen Magnetic Particle Separation Device - (Peer Reviewed Journal)
Nou, X., Arthur, T.M., Bosilevac, J.M., Harhay, D.M., Guerini, M.N., Kalchayanand, N., Koohmaraie, M. 2006. Improvement of immunomagnetic separation for Escherichia coli O157:H7 detection by the pickpen magnetic particle separation device. Journal of Food Protection 69:2870-2874.
Treatments Using Hot Water Instead of Lactic Acid Reduce Levels of Aerobic Bacteria and Enterobacteriaceae and Reduce the Prevalence of Escherichia Coli O157:h7 on Preevisceration Beef Carcasses - (Peer Reviewed Journal)
Bosilevac, J.M., Nou, X., Barkocy-Gallagher, G.A., Arthur, T.M., Koohmaraie, M. 2006. Treatments using hot water instead of lactic acid reduce levels of aerobic bacteria and Enterobacteriaceae and reduce the prevalence of Escherichia coli O157:H7 on preevisceration beef carcasses. Journal of Food Protection. 69(8):1808-1813.
Methods for Recovering Escherichia Coli O157:h7 from Cattle Fecal, Hide, and Carcass Samples: Sensitivity and Improvements - (Peer Reviewed Journal)
Barkocy-Gallagher, G.A., Edwards, K.K., Nou, X., Bosilevac, J.M., Arthur, T.M., Shackelford, S.D., Koohmaraie, M. 2005. Methods for recovering Escherichia coli O157:H7 from cattle fecal, hide, and carcass samples: Sensitivity and improvements. Journal of Food Protection. 68:2264-2268.
Evaluation of Culture and Pcr-Based Detection Methods for Escherichia Coli O157:h7 in Inoculated Ground Beef - (Peer Reviewed Journal)
Arthur, T.M., Bosilevac, J.M., Nou, X., Koohmaraie, M. 2005. Evaluation of culture- and PCR-based detection methods for Escherichia coli O157:H7 in inoculated ground beef. Journal of Food Protection. 68:1566-1574.
Efficacy of Ozonated and Electrolyzed Oxidative Waters to Decontaminate Hides of Cattle Before Slaughter - (Peer Reviewed Journal)
Bosilevac, J.M., Shackelford, S.D., Brichta, D.M., Koohmaraie, M. 2005. Efficacy of ozonated and electrolyzed oxidative waters to decontaminate hides of cattle before slaughter. Journal of Food Protection. 68:1393-1398.
Effects of Low-Dose, Low-Penetration Electron Beam Irradiation of Chilled Beef Carcass Surface Cuts on Escherichia Coli O157:h7 and Meat Quality - (Peer Reviewed Journal)
Arthur, T.M., Wheeler, T.L., Shackelford, S.D., Bosilevac, J.M., Nou, X., Koohmaraie, M. 2005. Effects of low-dose, low-penetration electron beam irradiation of chilled beef carcass surface cuts on Escherichia coli O157:H7 and meat quality. Journal of Food Protection. 68(4):666-672.
Development and Evaluation of An on-Line Hide Decontamination Procedure for Use in a Commercial Beef Processing Plant - (Peer Reviewed Journal)
Bosilevac, J.M., Nou, X., Osborn, M.S., Allen, D.M., Koohmaraie, M. 2005. Development and evaluation of an on-line hide decontamination procedure for use in a commercial beef processing plant. Journal of Food Protection. 68(2):265-272.
Decreased Dosage of Acidified Sodium Chlorite Reduces Microbial Contamination and Maintains Organoleptic Qualities of Ground Beef Products - (Peer Reviewed Journal)
Bosilevac, J.M., Shackelford, S.D., Fahle, R., Biela, T., Koohmaraie, M. 2004. Decreased dosage of acidified sodium chlorite reduces microbial contamination and maintains organoleptic qualities of ground beef products. Journal of Food Protection 67:2248-2254.
Escherichia Coli O157 Prevalence and Enumeration of Aerobic Bacteria, Enterobacteriaceae, and Escherichia Coli O157 at Various Steps in Commercial Beef Processing Plants - (Peer Reviewed Journal)
Arthur, T.M., Bosilevac, J.M., Nou, X., Shackelford, S.D., Wheeler, T.L., Kent, M.P., Jaroni, D., Pauling, B., Allen, D.M., Koohmaraie, M. 2004. Escherichia coli O157 prevalence and enumeration of aerobic bacteria, Enterobacteriacae, and Escherichia coli O157 at various steps in commercial beef processing plants. Journal of Food Protection. 67(4):658-665.
Prevalence of Escherichia Coli O157 and Levels of Aerobic Bacteria and Enterobacteriaceae Are Reduced When Hides Are Washed and Treated with Cetylpyridinium Chloride at a Commercial Beef Processing Plant - (Peer Reviewed Journal)
Bosilevac, J.M., Arthur, T.M., Wheeler, T.L., Shackelford, S.D., Rossman, M., Reagan, J.O., Koohmaraie, M. 2004. Prevalence of Escherichia coli O157 and levels of aerobic bacteria and Enterobacteriaceae are reduced when hides are washed and treated with cetylpyridinium chloride at a commercial beef processing plant. Journal of Food Protection. 67(4):646-650.
Protocol for Evaluating the Efficacy of Cetylpyridinium Chloride As a Beef Hide Intervention - (Peer Reviewed Journal)
Bosilevac, J.M., Wheeler, T.L., Rivera-Betancourt, M., Nou, X., Arthur, T.M., Shackelford, S.D., Kent, M.P., Jaroni, D., Osborn, M.S., Rossman, M., Reagan, J.O., Koohmaraie, M. 2004. Protocol for evaluating the efficacy of cetylpyridinium chloride as a beef hide intervention. Journal of Food Protection. 67(2):303-309.
Effect of Chemical Dehairing on the Prevalence of Escherichia Coli O157:h7 and the Levels of Aerobic Bacteria and Enterobacteriaceae on Carcasses in a Commercial Beef Processing Plant - (Peer Reviewed Journal)
Nou, X., Rivera-Betancourt, M., Bosilevac, J.M., Wheeler, T.L., Shackelford, S.D., Gwartney, B.L., Reagan, J.O., Koohmaraie, M. 2003. Effect of chemical dehairing on the prevalence of Escherichia coli O157:H7 and the levels of aerobic bacteria and Enterobacteriaceae on carcasses in a commercial beef processing plant. Journal of Food Protection. 66(11):2005-2009.
ARS News Articles
Last Modified: 4/20/2014
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