|An Integrated Genetic Linkage Map of the Soybean|
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The most recent published version of the soybean transcript map is located at this website. In addition, a map is now being prepared that will contain 3792 Single Nucleotide Polymorphism (SNP) markers, 1009 Simple Sequence Repeat (SSR) markers and 664 Restriction Fragment Length Polymorphism (RFLP) markers.
Newly developed SSRs were mapped in one or more of the five soybean populations: Minsoy × Noir 1, Minsoy × Archer, Noir 1× Archer, Clark × Harosoy, and A81-356022 × PI468916. A JoinMap analysis resulted in a map with 20 linkage groups containing a total of 1849 markers, including 1015 SSRs, 709 RFLPs, 73 RAPDs, 23 classical traits and 29 others. Among these, 417 were new SSRs to the maps constructed by Cregan et al. (1999a). Two hundred of these new loci were developed in collaboration with the Monsanto Co. From 12 to 29 new SSR markers were added to each linkage group. Of the newly developed markers, 90 were positioned to 30 of the 36 gaps of 20 cM or more that had existed in the previous version of the map (Cregan et al. 1999).
Five widely used soybean mapping populations were used for SSR positioning:
DNA, Isozyme and Classical Markers:
Linkage map construction:
Cregan PB, Jarvik T, Bush AL, Shoemaker RC, Lark KG, Kahler AL, Kaya N, VanToai TT, Lohnes DG, Chung J, Specht JE (1999) An integrated genetic linkage map of the soybean. Crop Sci. 39:1464-1490.
Keim P, Diers BW, Olson TC, and Shoemaker RC (1990) RFLP mapping in soybean: association between marker loci and variation in quantitative traits. Genetics 126:735-742.
Mansur, LM, Orf JH, Chase K, Jarvik T, Cregan PB, Lark KG (1996) Genetic mapping of agronomic traits using recombinant inbred lines of soybean. Crop Sci. 36:1327-1336.
Mansur, LM and Orf J (1995) Evaluation of soybean recombinant inbreds for agronomic performance in northern USA and Chile. Crop Sci. 35:422-425.
Shoemaker, RC and Olson TC (1993) Molecular linkage map of soybean (Glycine max L. Merr.). p. 6.131-6.138. In O’Brien SJ (ed.) Genetic maps: Locus maps of complex genomes. Cold Spring Harbor Laboratory Press, New York.
Shoemaker, RC and Specht JE (1995) Integration of the soybean molecular and classical genetic linkage groups. Crop Sci. 35:436-446.
Stam P (1993) Construction of integrated genetic linkage maps by means of a new computer package: JoinMap. The Plant Journal 3(5):739-744.
Van Ooijen, JW and Voorrips RE (2001) JoinMap 3.0 software for the calculation of genetic linkage maps. Plant Research Internation, Wageningen, the Netherlands.