Dr. Kable is interested in the mechanisms governing how diet impacts the bacterial composition of the human gut and how these diet-bacterial interactions can influence human health. In particular, she is interested in how dietary fiber can affect the composition of the gut microbiota in such a way as to increase or decrease colonization resistance and susceptibility to food borne pathogens.
The Kable Lab’s research is currently focused on (1) characterization of the gut microbiota in a healthy human cohort and potential interactions between this composition and the long term dietary habits of healthy individuals, (2) optimization of methods for medium through-put culture of diverse gut microbiota, (3) in vitro fermentation of complete gut microbiota and individual bacteria from healthy human subjects with various food components, especially dietary fiber.
Assessing the Impact of Diet on Inflammation in Healthy and Obese Adults in a Cross-Sectional Phenotyping and a Longitudinal Intervention Trial (USDA Agricultural Research Service)
Charles Stephensen (PI), 10/01/13-10/01/18
The goal of this study is to identify lifestyle, biological, and environmental factors associated with the variance in metabolic health and immune responses to dietary challenges in generally healthy US adults. The role of the Kable Lab in this project is to characterize the gut microbial community of each subject enrolled in this study and understand the relationships between diet and other lifestyle factors.
Milk, Health, and Genetics (California Dairy Research Foundation
Danielle Lemay (PI), 01/01/17-12/31/18
The overall goal of this project is to understand how lactase persistence genotypes and dairy consumption interact to impact human health. The Kable lab is examining gut microbiota of individuals relative to their lactase persistence genotype and level of fluid milk consumption.
ADDITIONAL CURRENT COLLABORATIONS:
- Carrie Waterman, UC Davis Extension and USDA Agricultural Research Service, WHNRC, Effects of bacterial fermentation products of Moringa oliefera on gastrointestinal epithelial cells
- Danielle Lemay, USDA Agricultural Research Service, WHNRC, Fecal metatranscriptomics of macaques with idiopathic chronic diarrhea reveals a dysfunctional microbiome with impaired substrate consumption
- Nancy Keim, USDA Agricultural Research Service, WHNRC, Fibermalt clinical trial data analysis
- Liping Huang, USDA Agricultural Research Service, WHNRC, Study on zinc deficient Znt7 knockout mice
Mary E. Kable, Ph.D.
Visiting Assistant Professor, UC Davis Department of Nutrition
Office: WHNRC Rm 209, (530)752-1607
Lab: WHNRC Rm 235
Email address: email@example.com
Dana De Vries
Biological Science Technician
Lab: WHNRC Rm 235, (530)752-4725
Email address: firstname.lastname@example.org
Graduate Student Researcher, UC Davis Department of Nutrition
Office: WHNRC Rm 108
Email address: email@example.com
Chemist (Support Scientist), point of collaboration with Lemay Lab
Lab: WHNRC Rm 235, (530)752-4725
Email address: firstname.lastname@example.org
METHODS AND TECHNIQUES
The Kable Lab focuses on methods for the analysis of human gut microbes:
For growth and fermentation experiments we use a:
- 2.7’ x 6.5’ Coy vinyl anaerobic chamber with space for two people to work, equipped with
- oxygen/hydrogen monitor,
- hydrogen sulfide removal system,
- and SPECTROstar Nano full UV/Vis spectrum absorbance microplate reader with cuvette port
Endpoints analyzed in the Kable Lab:
- 16S rRNA sequence based assessment of human gut microbial communities
- Cultured bacterial isolates from human gut samples
- Bacterial growth curves, antibiotic susceptibility, etc.
- Trans-epithelial resistance of colonic epithelial cells after exposure to microbial fermentation products
RECENT PUBLICATIONS (Selected)
Kable ME, LM Hansen, C Styer, S Deck, A Shetsova, K Eaton, M Martin, T Borén and JV Solnick, (2017) “Host Determinants of Expression of the Helicobacter pylori BabA Adhesin” Scientific Reports 7:46499. doi:10.1038/srep46499
Cooper DN, ME Kable, ML Marco, A De Leon, B Rust, JE Baker, W Horn, D Burnett and NL Keim, (2017) “The Effects of Moderate Whole Grain Consumption on Fasting Glucose and Lipids, Gastrointestinal Symptoms, and Microbiota” Nutrients 9(2):173. doi:10.3390/nu9020173
Zaragoza J, Z Bendiks, C Tyler, ME Kable, TR Williams, Y Luchkovska, E Chow, K Boundy-Mills and ML Marco, (2017) “Effects of Exogenous Yeast and Bacteria on the Microbial Population Dynamics and Outcomes of Olive Fermentations” mSphere 2(1):e00315-16. doi:10.1128/mSphere.00315-16
Kable ME, Y Srisengfa, M Laird, J Zaragoza, J McLeod, J Heidenreich and ML Marco, (2016) “The core and seasonal microbiota of raw bovine milk in tanker trucks and the impact of transfer to a milk processing facility” mBio 7(4): e00836-16. doi: 10.1128/mBio.00836-16
Hald S and AG Schioldan, ME Moore, A Dige, HN Lærke, J Agnholt, KE Bach Knudsen, K Hermansen, M Marco, S Gregersen and JF Dahlerup, (2016) “Effects of Arabinoxylan and Resistant Starch on Intestinal Microbiota and Short-Chain Fatty Acids in Subjects with Metabolic Syndrome: A Randomized Crossover Study” PLoS ONE 11(7): e0159223. doi:10.1371/journal.pone.0159223
Kieffer DA, BD Piccolo, ND Vaziri , S Liu , WL Lau, M Khazaeli, S Nazertehrani, ME Moore, ML Marco, RJ Martin and SH Adams, (2016) “Resistant Starch Alters Gut Microbiota, Uremic Retention Solutes and Xeno-Metabolite Profiles Concurrent with Amelioration of Chronic Kidney Disease in Rats” Journal of the American Society of Nephrology 310: F857-F871. doi:10.1152/ajprenal.00513.2015
Moore ME, A Lam, S Bhatnagar and J Solnick, (2013) “Environmental Determinants of Transformation Efficeincy in Helicobacter pylori.” Journal of Bacteriology 196 (2) 337-344.