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	Bruker Reflex II MALDI: MS Analysis Peptide mass mapping High MW peptides or intact proteins
	Finnigan LCQ Ion Trap: LC/MS/MS Peptide sequencing by MS/MS Effective for mixtures of proteins 96-well format, automated
	Sciex QSTAR O-MALDI: MS/MS Peptide mass mapping MS/MS peptide sequencing Post translational modifications 96-well format, automated

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Notes

Proteins in the wheat endosperm proteome are identified using mass spectrometry. Peptides produced from 2D gel spots are analyzed utilizing different mass spectrometers. Initial identifications by MALDI-TOF MS (peptide mass mapping) were carried out using a Bruker Reflex II (Bruker Daltonics, Billerica, MA). Subsequent identifications by MS/MS were performed using either a Finnigan LCQ Classic or an API QSTAR Pulsar i hybrid quadrupole-TOF instrument. MS and MS/MS experiments were performed with the QSTAR configured either with the ESI source or the o-MALDI source.

References

Balmer, Y., Vensel, W.H., Cai, N., Manieri, W., Schürmann, P., Hurkman, W.J., Buchanan, B.B. 2006. A complete ferredoxin/thioredoxin system regulates fundamental processes in amyloplasts. PNAS. 103: 2988-2993.

Holmes-Davis R., Tanaka C.K., Vensel W.H., Hurkman W.J., and McCormick S. 2005. Proteome mapping of mature pollen of Arabidopsis thaliana. Proteomics 5: 4864-4884.

Vensel, W.H., Harden, L., Tanaka, C.K., Hurkman, W.J. and Haddon, W.F. 2002. Identification of wheat endosperm proteins by MALDI Mass Spectrometry and LC-MS/MS. J. Biomolecular Techniques 13(3): 95-100.

Vensel, W.H., Tanaka, C.K., Cai, N., Wong, J.H., Buchanan, B.B. and Hurkman, W.J. 2005. Developmental changes in the metabolic protein profiles of wheat endosperm. Proteomics 5( ): 1594-1611.

Wong, J.H., Balmer, Y., Cai, N., Tanaka, C.K., Vensel, W.H., Hurkman, W.J., Buchanan, B.B. 2003. Unraveling thioredoxin-linked metabolic processes of cereal starchy endosperm using proteomics. FEBS Letters 454(1-3): 151-156.

 

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