Figure 126. Lateral submedial longitudinal section of Heterodera glycines J2 near a feeding site at 5 hours after inoculation. Stylet is retracted. Pm, protractor muscle; Sm, somatic muscle. Bar=1.
Figure 128. Longitudinal section of J2 at 1 hour after inoculation, showing segment of gland extensions that typify secretory granule morphology throughout subventral gland cell and extension (Svx). Adjacent to subventral gland extension, enlarged lumen of esophago-intestinal valve (Eiv) indicates that nematode is in feeding mode. Bar=1.0 µm.
Figure 130. Nucleus (N) in subventral gland cell, surrounded by Golgi (GA) and numerous secretory granules (SG) with various levels of electron density. Bar=1.0 µm.
Figure 131. Section through subventral gland, showing Golgi with cisternae stacks (arrows) facing each other. Condensing vesicles (cV) merge to form secretory granules (SG). Bar=1.0 µm.
Figure 133. Secretory granules within ampulla are electron-translucent except for small residues of electron-opaque material (Sgr). Svv, subventral gland valve. Bar=1.0 µm.
Figure 134. Electron-opaque secretory granules (SG) accumulated at anterior of gland body. Some granules contain electron-opaque regions (arrows) within their granule matrices. Bar=1.0 µm.
Figure 136. Electron-opaque secretory granules (SG) within subventral gland ampulla (SvA), before transfer of contents into esophageal lumen via subventral gland valve (Svv). Bar=1.0 µm.
Figure 138. Transverse section through gland extensions anteriad from nerve ring, showing secretory granules (SG) of subventral gland extensions (Svx) with small dense cores. In contrast, secretory granules of dorsal gland extension (Dx) are larger and moderately electron opaque. Bar=1.0 µm.
Figure 139. Transverse section of J2 at 3 days after inoculation, showing closed subventral gland valves (Svv) and accumulations of secretory granules (SG) within the ampullae and near the valves. Larger secretory granules occur in dorsal gland extension (Dx). Mt, microtubules. Bar=1.0 µm.
Figure 141.Transverse section through a subventral gland at 4 days after inoculation, showing electron-translucent flocculent secretory bodies (FSB) within dense matrix of cytoplasm. Mc, mitochondria. Bar=1.0 µm.
Figure 143. Longitudinal section through portions of dorsal and subventral glands of Heterodera glycines J3 at 6 days after inoculation. Nuclei of dorsal gland (DN) and of one subventral gland (SvN) are seen. Moderate-sized flocculent secretory bodies (FSB) with various levels of electron density are distributed throughout subventral gland. Bar=1.0 µm.
Figure 145. Open subventral gland valves (Svv) of Heterodera glycines J3 at 8 days after inoculation, filled with electron-opaque material similar to contents of adjacent secretory granules (SG) in ampullae. Small, relatively electron-opaque secretory granules show a sharp contrast to secretory granules in subventral glands of H. glycines J2 during shorter infection periods, as described in fig. 144. Bar=1.0 µm.
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