Jocelyn K.C. Rose (PI), Department of Plant Biology, Cornell University
Theodore W. Thannhauser (co-PI) USDA-ARS-NAA-PSNR, Ithaca, NY
James Giovannoni (collaborator), USDA-ARS-NAA-PSNR, Ithaca, NY
Lukas Mueller (collaborator) Department of Plant Breeding, Cornell University
Scientific Objectives and Approaches: A substantial portion of the plant proteome is localized in the cell wall/apoplast. This is not surprising, given the central role of the wall in many aspects of plant biology, including cell growth and differentiation, defence, metabolism and transport of nutrients and responses to environmental stresses. However, the complement of proteins that are secreted to the wall, or "secretome", is far less characterized than those of other plant subcellular compartments. This proposal outlines an integrated approach to catalog the tomato secretome, coupling a suite of newly developed functional screens and sequencing of highly purified wall protein extracts, with bioinfomatic tools and computational prediction. This infrastructure will be used to evaluate the qualitative and quantitative dynamics of the wall proteome during two biological processes that are intimately associated with wall biology: fruit ripening and plant defence responses. The specific objectives of this project are:
An important element of this project is the development of a strong education and training component. In addition to the expected training of graduate and undergraduate students, this will take the form of two new programs: (a) a Plant Genome Research internship program that has been created as a Cornell-wide initiative to bring together a critical mass of undergraduate students, high school students and high school teachers on the Cornell campus each summer. These individuals will participate in lab-based research and mentoring within the larger genomics-related community of plant scientists; (b) an annual summer Proteomics Workshop, comprising a seminar series, poster-presentations and lab-based practical training. In both cases, the participation of under-represented groups at the undergraduate and graduate levels will be ensured through the support of a network of existing Cornell programs. The project will generate a valuable, publicly available resource in the form of a webbased plant secretome database thus ensuring rapid public dissemination of the data. Importantly, this proposal will take full advantage of, and enhance, previous investments by the NSF in tomato genomics and provide a template for similar studies in other plant species, many of which are agriculturally and commercially important.