Skip to main content
ARS Home » Northeast Area » Ithaca, New York » Robert W. Holley Center for Agriculture & Health » Emerging Pests and Pathogens Research » Docs » Louela Castrillo

Louela Castrillo
headline bar




Louela A. Castrillo

Microbiologist and Acting Curator, USDA ARS Collection of Entomopathogenic Fungal Cultures


Phone: (607) 255-7008
Fax: 607 255 1132



Ph.D., North Carolina State University (Entomology/Genetics), 1997
M.S., North Carolina State University (Entomology/Microbiology), 1993


Fungal pathogens of arthropods and other invertebrates
Curates the USDA-ARS Collection of Entomopathogenic Fungal Cultures (ARSEF), the world's largest and most taxonomically diverse germplasm repository for fungal pathogens of insects, mites, spiders, nematodes, and other invertebrates. This collection currently comprises over 14,300 isolates representing over 700 fungal taxa isolated from over 1300 arthropod hosts, representing major insect orders, and 1800 locations on every continent. 


Selected Publications

Castrillo, L. A., Wraight, S. P., Galaini-Wraight, S., Matsumoto, T. K. and Keith, L.  2020. Diverse haplotypes of naturally occurring Beauveria bassiana in populations of coffee berry borer on the island of Hawaii. (submitted)

Wu, S., Toews, M. D., Castrillo, L. A., Barman, A. K., Cottrell, T. E., Shapiro-Ilan, D. I. 2020. Virulence of a new strain of Isaria javanica isolated from a natural epizootic in the silverleaf whitefly, Bemisia tabaci (biotype B). (submitted)

Manfrino, R. G., Castrillo, L. A., Lopez Lastra, C. C., Toledo, A. V., Ferrari, W., Jensen, A. B. 2020. Morphological and molecular characterization of Entomophthorales (Entomophthoromycota: Entomophthoromycotina) from Argentina. Acta Mycologica (in press).

Hajek, A. E, Castrillo, L. A.  2020. Relationship with symbionts. In Biology and Ecology of Sirex noctilio and Prospects for Biological Control in North America (Haavik, L., Hajek, A. E., Stephen, F., Eds.)  FHAAST

Clifton, E. H., Castrillo, L. A., Gryganski, A., Hajek. A. E.  2019. Native fungal pathogens drive collapse of a new invasive herbivore PNAS 116, 9178-9180.

Hajek, A. E, Steinkraus, D. C., Castrillo, L. A. 2018. Sleeping beauties: Horizontal transmission via resting spores of species in the Entomophthoromycotina. In Mechanism Underlying Transmission of Insect Pathogens (van Oers, M. M., and Eilenberg, J., Eds.) Insects (doi:10.3390/insects9030102)

Wraight, S. P., Galaini-Wraight, S., Castrillo, L., Keith, L., Matsumoto, T. 2018. Isolation, culture, and identification of Hirsutella from coffee berry borer in Hawaii.  Journal of Invertebrate Pathology 157, 53-66. (

Wraight, S. P., Galaini-Wraight, S., Castrillo, L., Keith, L., Matsumoto, T. 2018. Prevalence of wild type strains of Beauveria bassiana in populations of coffee berry borer on the island of Hawaii. Journal of Invertebrate Pathology 156, 54-72

Schneider, J. C., Leppla, N. C., Chaudhury, M. F, Castrillo, L. A., Ng, S., Fisher, W. R., Ebling, P. M., Caprio, M. A., and Riddell, T. 2018. Educating the next generation of insect rearing professionals. American Entomologist 64, 102-111.

Castrillo, L. A.  2017.  The (Insect) Host Population. In Ecology of Invertebrate Diseases (Hajek, A. E. and    Shapiro-Ilan, D., Eds.) pp. 99-139. John Wiley & Sons

Castrillo, L. A., Mayfield, A. E., Griggs, M. H., Camp, R., Taylor, A, Mudder, B., and Vandenberg, J. D.  2017. Mortality and reduced brood production in walnut twig beetles, Pityophthorus juglandis (Coleoptera: Curculionidae), following exposure to commercial strains of Beauveria bassiana and Metarhizium brunneum. Biological Control 114, 79-86.

Fisher, J. J., Castrillo, L. A., Donzelli, B. G.G., Hajek, A. E.  2017.  Starvation and imidacloprid exposure influence immune response by Anoplophora glabripennis (Coleoptera: Cerambicidae) to a fungal pathogen. Journal of Economic Entomology (doi: 10.1093/jee/tox124)

Castrillo, L. A., Griggs, M. H., and Vandenberg, J. D.  2016.  Competitions between biological control fungi and fungal symbionts associated with ambrosia beetles Xylosandrus crassiusculus and X. germanus (Coleoptera: Curculionidae): mycelial interactions and impact on brood production.  Biological Control 103, 138-146.

Montalva, C., dos Santos, K., Collier, K., Rocha, L. F. N., Castrillo, L. A., Fernandes, E. K. K., Luz, C., Humber, R. A.  2016.  First report of Leptolegnia chapmanii (Peronosporomycetes; Saprolegniales) affecting mosquitoes in Brazil.  Journal of Invertebrate Pathology 136, 109-116.

Cooperband, M. F., Stouthamer, R., Carrillo, D., Eskalen, A., Thibault, T., Cossé, A. A., Castrillo, L. A., Vandenberg, J. D., and Rugman-Jones, P. F.  2016.  Biology of two members of the Euwallacea fornicatus species complex (Coleoptera: Curculionidae: Scolytinae), recently invasive in the USA, reared on an ambrosia beetle artificial diet.  Agricultural and Forest Entomology DOI:10.1111/afe.12155

Castrillo, L. A., Hajek, A. E, Pajares, J. A, Thomsen, I. M., Csoka, G., Kenaley, S., Kepler, R. M., Zamora, P., Angeli, S. P.  2015.  Multilocus genotyping of Amylosterum spp. associated with Sirex noctilio and other woodwasps from Europe reveal clonal lineage introduced to the US.  Fungal Biology 119, 595-604.

Mayers, C. G., McNew, D. L., Harrington, T. C., Roeper, R. A., Fraedrich, S. W., Ciedermann, P. H. W., Castrillo, L. A., Reed, S.  2015.  Three genera in the Ceratocystidaceae are the respective symbionts of three independent lineages of ambrosia beetles with large, complex mycangia.  Fungal Biology 119, 1075-1092

Castrillo, L. A., Hajek, A. E. 2014. Detection of presumptive mycoparasites associated with Entomophaga maimaiga resting spores in forest soils. Journal of Invertebrate Pathology 124, 87-89

Castrillo, L. A., Griggs, M. H., and Vandenberg, J. D.  2013.  Granulate ambrosia beetle, Xylosandrus crassiusculus (Coleoptera: Curculionidae), survival and brood production following exposure to entomopathogenic and mycoparasitic fungi.  Biological Control 67, 220-226.

Hajek, A. E., Nielsen, C., Kepler, R. M., and Castrillo, L. A.  2013.  Fidelity among Sirex woodwasps and their fungal symbionts.  Molecular Ecology 65, 753-762.

Castrillo, L. A., Griggs, M. H., and Vandenberg, J. D.  2012.  Brood production by ambrosia beetle Xylosandrus germanus (Coleoptera: Curculionidae) and growth of its fungal symbiont on artificial diet made with sawdust from different tree species.  Environmental Entomology 41, 822-827 

Gibson, D. M., Castrillo, L.A., Donzelli, B. G. G., and Milbrath, L. R.  2012.  First report of blight caused by Sclerotium rolfsii on the invasive exotic weed, Vincetoxicum rossicum (pale swallow-wort) in western New York.  Plant Disease (DOI: 10.1094/PDIS-08-11-0692)

Castrillo, L. A., Griggs, M. H., Ranger, C. M., Reding. M. E., and Vandenberg, J. D.  2011.  Virulence of commercial strains of Beauveria bassiana and Metarhizium brunneum (Ascomycota: Hypocreales) against adult Xylosandrus germanus (Coleoptera: Curculionidae) and impact on brood.  Biological Control 58, 121-126.

Braun, S. E., Castrillo, L. A., Sanderson, J. P., Daughtrey, M. L., and Wraight, S. P.  2010.  Transstadial transmission of Pythium in Bradysia impatiens and lack of larval vectoring capacity. Phytopathology 100, 1307-1313.

Castrillo, L. A. Liu, H.-P., Bauer, L. S., Griggs, M. H., and Vandenberg, J. D.  2010.  Characterization of Beauveria bassiana (Ascomycota: Hypocreales) isolates associated with Agrilus planipennis (Coleoptera: Buprestidae) populations in Michigan.  Biological Control 54, 135-140.

Castrillo, L. A., Griggs, M. H., Liu, H.-P., Bauer, L. S., and Vandenberg, J. D.  2010.  Assessing deposition and persistence of Beauveria bassiana GHA (Ascomycota: Hypocreales) applied for control of the emerald ash borer, Agrilus planipennis (Coleoptera: Buprestidae), in a commercial nursery.  Biological Control 54, 61-67

Castrillo, L. A. and Humber, R. A.  2009.  Fungi: Identification and diagnosis.  In Molecular Approaches and Techniques for the Study of Insect Pathogens (Stock, S. P., Glazer, I., Boemare, N., and Vandenberg, J. D., Eds. pp. 50-70. CABI Publishing, Wallingford, UK.  

Castrillo, L. A., Griggs, M. H., and Vandenberg, J. D.  2008. Quantitative field detection of Beauveria bassiana GHA (Ascomycota: Hypocreales), a potential microbial control agent of the emerald ash borer, by real-  time PCR. Biological Control 45, 163-169.

Castrillo, L. A., Ugine, T. A., Filotas, M. J., Sanderson, J., Vandenberg, J. D., and Wraight, S. P.  2008. Molecular characterization and comparative virulence of Beauveria bassiana isolates (Ascomycota: Hypocreales) associated with the greenhouse shore fly, Scatella tenuicosta (Diptera: Ephydridae). Biological Control 45, 154-162.

Castrillo, L. A., Thompson, L., Juneja, P., and Hajek, A. E.  2007.  Detection and quantification of Entomophaga maimaiga resting spores in forest soil using real-time PCR.  Mycological Research 111, 324-331.

Filotas, M. J., Castrillo, L. A., Vandenberg, J. D., Sanderson, J., and Wraight, S. P.  2005.  Novel isolates of the entomopathogenic fungus Beauveria bassiana for biological control of the shore fly, Scatella tenuicosta IOBC/WPRS Bull.  28, 95-98.

Castrillo, L. A., Roberts, D. W. and Vandenberg, J. D.  2005.  The fungal past, present, and future: Germination, ramification and reproduction.  Journal of Invertebrate Pathology 89, 46-56.

Castrillo, L. A., Griggs, M. H., and Vandenberg, J. D.  2004.  Vegetative compatibility groups in indigenous and mass-released strains of the entomopathogenic fungus Beauveria bassiana: likelihood of recombination in the field.  Journal of Invertebrate Pathology  86, 26-37

Castrillo, L. A., Vandenberg, J. D., and Wraight, S. P.  2003.  Strain-specific detection of introduced Beauveria bassiana in agricultural fields by use of sequence-characterized amplified region markers. Journal of  Invertebrate Pathology  82, 75-83.

Lee, R. E., Castrillo, L. A., Lee, M. R., Wyman, J. A., and Costanzo, J. P.  2001.  Using Ice nucleating bacteria to reduce winter survival of Colorado potato beetles: Development of a novel strategy for biological control. In Insect Timing: Circadian Rhythmicity and Seasonality (D. L. Denlinger, J. M. Giebultowicz, and D. S. Saunders, Eds.) pp. 213- 227.  Elsevier, New York.

Humphreys, T. L., Castrillo, L. A., Lee, M. R.  2001. Sensitivity of partially purified ice nucleation activity of Fusarium avenacium SRSF 616.  Current Microbiology 42, 330-338.

Castrillo, L. A., Rutherford, S. T., Lee, R. E., and Lee, M. R.  2001.  Enhancement of ice-nucleating activity in Pseudomonas fluorescens and its effect on efficacy against Colorado potato beetles.  Biological Control 21, 27-34.

Castrillo, L. A., Lee, R. E., Wyman, J. A., Lee, M. R. and Rutherford, S. T.  2001.  Field persistence of ice-nucleating Pseudomonas fluorescens in overwintering Colorado potato beetles. Biological Control 21, 11-18.

Castrillo, L. A., Lee, R. E., Lee, M. R. and Rutherford, S. T.  2000.  Identification of ice-nucleating active bacteria for biological control of overwintering Colorado potato beetles.  Journal of Economic Entomology 93, 226-233.

Castrillo, L. A., Lee, R. E., Lee, M. R., and Wyman, J. A.  2000.  Long-term retention of the ice nucleating Pseudomonas fluorescens by overwintering Colorado potato beetles.  Cryo-Letters 21, 5-12.

Castrillo, L. A., Wiegmann, B. M., and Brooks, W. M.  1999.  Genetic variation in Beauveria bassiana populations associated with the darkling beetle, Alphitobius diaperinus, in poultry houses. Journal of Invertebrate Pathology 73, 269-275

Castrillo, L. A. and Brooks, W. M.  1998.  Differentiation of Beauveria bassiana isolates from the darkling beetle, Alphitobius diaperinus, using isozyme and RAPD analyses.  Journal of Invertebrate Pathology 72, 190-196.

Castrillo, L. A., Stringham, M., Arends, J. J., and Brooks, W. M.  1994.  Prevalence of protozoan infections in darkling beetles from poultry houses in North Carolina.  Journal of Invertebrate Pathology 63, 255-259.