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United States Department of Agriculture

Agricultural Research Service

Louela Castrillo
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Louela A. Castrillo
Louela.Castrillo@ars.usda.gov

Phone: (607) 255-7008
Fax: 607 255 1132

Microbiologist and Acting Curator

 

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Education
Ph.D., North Carolina State University (Entomology/Genetics), 1997
M.S., North Carolina State University (Entomology/Microbiology), 1993

 

Fungal pathogens of arthropods and other invertebrates

Curates the USDA-ARS Collection of Entomopathogenic Fungal Cultures (ARSEF), the world's largest and most taxonomically diverse germplasm repository for fungal pathogens of insects, mites, spiders, nematodes, and other invertebrates. This collection currently comprises over 13,300 isolates from almost 600 fungal taxa isolated from 1160 different hosts and 1800 locations on every continent. Provides identifications of specimens and cultures on request.

 

Selected Publications

Castrillo, L. A., Griggs, M. H., Oliver, J. B., Youssef, N. and Vandenberg, J. D.  2017. Efficacy of microbial control fungi against ambrosia beetles Xylosandrus crassiusculus and Cnestus mutilatus in field studies in Tennessee. (in prep)

Castrillo, L. A., Wraight, S. P., Galaini-Wraight, S., Matsumoto, T. K. and Keith, L.  2017.  Genetic diversity of  naturally occurring Beauveria bassiana in populations of coffee berry borer on the island of Hawaii. (in prep)

Wraight, S. P., Galaini-Wraight, S., Castrillo, L., Keith, L., Matsumoto, T. 2017. Isolation, culture, and identification of Hirsutella from coffee berry borer in Hawaii.  (in prep)

Wraight, S. P., Galaini-Wraight, S., Castrillo, L., Keith, L., Matsumoto, T. 2017. Prevalence of wild type strains of Beauveria bassiana in populations of coffee berry borer on the island of Hawaii. (in prep)

Castrillo, L. A.  2017.  The (Insect) Host Population. In Ecology of Invertebrate Diseases (Hajek, A. E. and Shapiro-Ilan, D., Eds.) pp. 99-139. John Wiley & Sons

Castrillo, L. A., Mayfield, A. E., Griggs, M. H., Camp, R., Taylor, A, Mudder, B., and Vandenberg, J. D.  2017. Mortality and reduced brood production in walnut twig beetles, Pityophthorus juglandis (Coleoptera: Curculionidae), following exposure to commercial strains of Beauveria bassiana and Metarhizium brunneum. Biological Control 114, 79-86.

Fisher, J. J., Castrillo, L. A., Donzelli, B. G.G., Hajek, A. E.  2017.  Starvation and imidacloprid exposure influence immune response by Anoplophora glabripennis (Coleoptera: Cerambicidae) to a fungal pathogen. Journal of Economic Entomology (doi: 10.1093/jee/tox124) 

Castrillo, L. A., Griggs, M. H., and Vandenberg, J. D.  2016.  Competitions between biological control fungi and  fungal symbionts associated with ambrosia beetles Xylosandrus crassiusculus and X. germanus (Coleoptera: Curculionidae): mycelial interactions and impact on brood production.  Biological Control 103, 138-146.

Montalva, C., dos Santos, K., Collier, K., Rocha, L. F. N., Castrillo, L. A., Fernandes, E. K. K., Luz, C., Humber  R. A.  2016.  First report of Leptolegnia chapmanii (Peronosporomycetes; Saprolegniales) affecting mosquitoes in Brazil.  Journal of Invertebrate Pathology 136, 109-116.

Cooperband, M. F., Stouthamer, R., Carrillo, D., Eskalen, A., Thibault, T., Cossé, A. A., Castrillo, L. A.,  Vandenberg, J. D., and Rugman-Jones, P. F.  2016.  Biology of two members of the Euwallacea   fornicatus species complex (Coleoptera: Curculionidae: Scolytinae), recently invasive in the USA, reared on an ambrosia beetle artificial diet.  Agricultural and Forest Entomology DOI:10.1111/afe.1215

Castrillo, L. A., Hajek, A. E, Pajares, J. A, Thomsen, I. M., Csoka, G., Kenaley, S., Kepler, R. M., Zamora, P., Angeli, S. P.  2015.  Multilocus genotyping of Amylosterum spp. associated with Sirex noctilio and other woodwasps from Europe reveal clonal lineage introduced to the US.  Fungal Biology 119, 595-604.

Mayers, C. G., McNew, D. L., Harrington, T. C., Roeper, R. A., Fraedrich, S. W., Ciedermann, P. H. W., Castrillo, L. A., Reed, S.  2015.  Three genera in the Ceratocystidaceae are the respective symbionts of         three independent lineages of ambrosia beetles with large, complex mycangia.  Fungal Biology 119, 1075-1092

Castrillo, L. A., Hajek, A. E. 2014. Detection of presumptive mycoparasites associated with Entomophaga maimaiga resting spores in forest soils. Journal of Invertebrate Pathology 124, 87-89

Castrillo, L. A., Griggs, M. H., and Vandenberg, J. D. 2013.  Granulate ambrosia beetle, Xylosandrus crassiusculus (Coleoptera: Curculionidae), survival and brood production following exposure to entomopathogenic and mycoparasitic fungi. Biological Control 67, 220-226

Hajek, A. E., Nielsen, C., Kepler, R. M., Long, S., and Castrillo, L. A.  2013.  Fidelity among Sirex woodwasps and their fungal symbionts.  Microbial Ecology 65, 753-762.

Castrillo, L. A., Griggs, M. H., and Vandenberg, J. D.  2012.  Brood production by ambrosia beetle Xylosandrus germanus (Coleoptera: Curculionidae) and growth of its fungal symbiont on artificial diet made with sawdust from different tree species. Environmental Entomology 41, 822-827.

Gibson, D. M., Castrillo, L. A., Donzelli, B. G. G., and Milbrath, L. R. 2011.  First report of blight caused by Sclerotium rolfsii on the invasive weed, Vincetoxicum rossicum (pale swallow-wort) in western New York.  Plant Disease (DOI: 10.1094/PDIS-08-11-0692)

Castrillo, L. A., Griggs, M. H., Ranger, C. M., Reding. M. E., and Vandenberg, J. D.  2011.  Virulence of commercial strains of Beauveria bassiana and Metarhizium brunneum (Ascomycota: Hypocreales) against adult Xylosandrus germanus (Coleoptera: Scolytidae) and impact on brood.  Biological Control 58. 121-26

Castrillo, L. A. Liu, H.-P., Bauer, L. S., Griggs, M. H., and Vandenberg, J. D.  2010. Beauveria bassiana (Ascomycota: Hypocreales) isolates associated with Agrilus planipennis (Coleoptera: Buprestidae) populations in Michigan. Biological Control 54, 135-140.

Castrillo, L. A., Griggs, M. H., Liu, H.-P., Bauer, L. S., and Vandenberg, J. D.  2010.  Assessing deposition and persistence of Beauveria bassiana GHA (Ascomycota: Hypocreales) applied for control of the emerald ash borer, Agrilus planipennis (Coleoptera: Buprestidae), in a commercial nursery.  Biological Control 54, 61-67.

Braun, S. E., Castrillo, L. A., Sanderson, J. P., Daughtrey, M. L., and Wraight, S. P. 2010. Transstadial transmission of Pythium in Bradysia impatiens and lack of larval vectoring capacity. Phytopathology 100, 1307-1313.

Castrillo, L. A. and Humber, R. A.  2009.  Identification and Diagnosis of Fungi.  In Insect Pathogens. Molecular Approaches and Techniques (Stock, S. P., Vandenberg, J. D., Glazer, I.,  and  Boemare, N., Eds).  CABI Publishing, Wallingford, UK.

Castrillo, L. A., Griggs, M. H., and Vandenberg, J. D.  2008.  Quantitative field detection of Beauveria bassiana GHA (Ascomycota: Hypocreales), a potential microbial control agent  of the emerald ash borer, by real-time PCR. Biological Control 45, 163-169.

Castrillo, L. A., Ugine, T. A., Filotas, M. J., Sanderson, J., Vandenberg, J. D., and Wraight, S. P.  2008.  Molecular characterization and comparative virulence of Beauveria bassiana isolates (Ascomycota: Hypocreales) associated with the greenhouse shore fly, Scatella tenuicosta (Diptera: Ephydridae).  Biological Control 45, 154-162.

Castrillo, L. A., Thomsen, L., Juneja, P. and Hajek, A. E. 2007.  Detection and quantification of Entomophaga maimaiga resting spores in forest soil using real-time PCR.  Mycological Research 111, 324-331.

Castrillo, L. A., Vandenberg, J. D., and Roberts, D. W. 2005. The fungal past, present and future: Germination, ramification and reproduction. Journal of Invertebrate Pathology 89, 46-56.

Filotas, M. J., Castrillo, L. A., Vandenberg, J. D., Sanderson, J., and Wraight, S. P. 2005. Novel isolates of the entomopathogenic fungus Beauveria bassiana for biological control of the shore fly, Scatella tenuicosta. IOBC /WPRS Bull. 28, 95-98.

Castrillo, L. A., Griggs, M. H., and Vandenberg, J. D. 2004. Vegetative compatibility groups in indigenous and mass-released strains of the entomopathogenic fungus Beauveria bassiana: likelihood of recombination in the field. Journal of Invertebrate Pathology 86, 26-37.

Castrillo, L. A., Vandenberg, J. D., and Wraight, S. P. 2003. Strain-specific detection of introduced Beauveria bassiana in agricultural fields by use of sequence-characterized amplified region markers. Journal of Invertebrate Pathology 82, 75-83.

Castrillo, L. A., Rutherford, S. T., Lee, R. E., and Lee, M. R. 2001. Enhancement of ice-nucleating activity in Pseudomonas fluorescens and its effect on efficacy against Colorado potato beetles. Biological Control 21, 27-34.

Castrillo, L. A., Lee, R. E., Wyman, J. A., Lee, M. R. and Rutherford, S. T. 2001. Field persistence of ice-nucleating Pseudomonas fluorescens in overwintering Colorado potato beetles. Biological Control 21, 11- 18.

Humphreys, T. L., Castrillo, L. A., Lee, M. R. 2001. Sensitivity of partially purified ice nucleation activity of Fusarium avenacium SRSF 616. Current Microbiology 42, 330-338.

Lee, R. E., Castrillo, L. A., Lee, M. R., Wyman, J. A., and Costanzo, J. P. 2001. Using Ice nucleating bacteria to reduce winter survival of Colorado potato beetles: Development of a novel strategy for biological control. In Insect Timing: Circadian Rhythmicity and Seasonality (D. L. Denlinger, J. M. Giebultowicz, and D. S. Saunders, Eds.) pp. 213- 227. Elsevier, New York.

Castrillo, L. A., Lee, R. E., Lee, M. R. and Rutherford, S. T. 2000. Identification of ice-nucleating active bacteria for biological control of overwintering Colorado potato beetles. Journal of Economic Entomology 93, 226-233.

Castrillo, L. A., Lee, R. E., Lee, M. R., and Wyman, J. A. 2000. Long-term retention of the ice nucleating Pseudomonas fluorescens by overwintering Colorado potato beetles. Cryo-Letters 21, 5-12.

Castrillo, L. A., Wiegmann, B. M., and Brooks, W. M. 1999. Genetic variation in Beauveria bassiana populations associated with the darkling beetle, Alphitobius diaperinus, in poultry houses. Journal of Invertebrate Pathology 73, 269-275.

Castrillo, L. A. and Brooks, W. M. 1998. Differentiation of Beauveria bassiana isolates from the darkling beetle, Alphitobius diaperinus, using isozyme and RAPD analyses. Journal of Invertebrate Pathology 72, 190-196.

 


Last Modified: 10/12/2017
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