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United States Department of Agriculture

Agricultural Research Service

Apple Collection
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1 - Apple Collection
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Apple bud cutting
Bud segments being weighed
Drying buds in lab freezer

1. Single bud section 35 mm long with healthy bud being cut from dormant well acclimated scion.

2. 10 bud segments being weighed prior to desiccation and samples placed on wire drying rack.
3. Buds dried at -40?C in lab freezer modified with controller and heat tape.
Buds dried to 30%
aluminum cryo boxes
Boxes of buds frozen to -30C
4. Buds dried to 30% moisture and sealed in heat shrinkable tubes with birch plugs in both ends.
5. Multiple tubes each containing 12 bud segments placed in aluminum cryo boxes.
6. Boxes with buds frozen to -30?C in programmable freezer.
Boxes transferred to LN storage
330 Boxes can be stored in each tank
7. Boxes transferred to LN storage tank and held at almost -170 ?C.
8. 330 boxes can be stored in each LN storage tank.

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Last Modified: 8/13/2016
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