1 - CENTRIFUGE SAFETY
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90% of centrifuge failures are the result of user errors. These errors may result in lost samples and damaged equipment as well as a risk to you the lab user and your lab.
Centrifuge Safety in Quicktime Movie
RunningTime: 8 minutes
Consult the owner's manual and centrifuge log (especially critical on ultra centrifuge), insure the tube fits properly in the rotor. This is important because up to 600,000 G forces may be generated during the centrifugation procedure.
Insure you are using the appropriate level of containment. Is the material potentially infectious and/or radioactive? If so, are you using aerosol containment tubes? Are you loading and unloading the rotor in a biological safety cabinet?
Suggested steps to follow BEFORE starting the centrifuge:
- Insure centrifuge bowl and tubes are dry.
- Is the centrifuge spindle clean?
- Avoid overfilling of tubes and bottles.
- Insure rotor is properly seated on drive hub.
- Make sure tubes are properly balanced in rotor (? gram at 1 G is roughly equivalent to 250 Kg @ 500,000 G's).
- Are O-rings properly attached to the rotor? Is the vacuum grease fresh?
- Has the rotor been properly secured to drive?
- Is the centrifuge lid shut properly?
After the above stops are taken and the centrifuge has started, make sure the run is proceeding normally before you leave the area.
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