Heping Cao, PHD
Research Biologist and Research Plant Physiologist,
Diet Genomics and Immunology Lab,
Beltsville Human Nutrition Research Center,
USDA, Agriculture Research Service
Publications (Peer-reviewed original articles)
Cao, H. and Anderson, R.A. Cinnamon has insulin-like and independent effects on gene expression in mouse adipocytes. Submitted, 2009.
Cao, H. and Lin, R. Quantitative evaluation of His-tag purification and immunoprecipitation of tristetraprolin and its mutant proteins from transfected human cells. Biotechnology Progress, in press, 2009.
Cao, H., Urban, Jr. J.F. and Anderson, R.A. Cinnamon polyphenol extract affects immune responses by regulating pro- and anti-inflammatory and glucose transporter gene expression in macrophages. Journal of Nutrition, 138:833-840, 2008.
Cao, H., Urban, Jr. J.F. and Anderson, R.A. Insulin increases tristetraprolin and decreases VEGF gene expression in mouse 3T3-L1 adipocytes. Obesity, 16:1208-1218, 2008.
Cao, H., Lin, R., Ghosh, S., Anderson, R.A. and Urban, Jr., J.F. Production and characterization of ZFP36L1 antiserum against recombinant protein from Escherichia coli, Biotechnology Progress, 24:326-333, 2008.
Cao, H. and Lin, R. Phosphorylation of recombinant tristetraprolin in vitro. Protein Journal, 27:163-169, 2008.
Cao, H., Polansky, M.M. and Anderson, R.A. Cinnamon extract and polyphenols affect the expression of tristetraprolin, insulin receptor, and glucose transporter 4 in mouse 3T3-L1 adipocytes. Archives of Biochemistry and Biophysics, 459:214-222, 2007.
Cao, H., Hininger-Favier, I., Kelly, M.A., Benaraba, R.,
Cao, H., Kelly, M.A., Kari, F., Dawson, H.D., Urban, Jr. J.F., Coves, S., Roussel, A.M. and Anderson, R.A. Green tea increases anti-inflammatory tristetraprolin and decreases pro-inflammatory tumor necrosis factor mRNA levels in rats (Highly accessed article). Journal of Inflammation, 4:1.1-12, 2007.
Cao, H., Deterding, L.J., Venable, J.D., Kennington, E.A., Yates, J.R. 3rd, Tomer, K.B. and Blackshear, P.J. Identification of the anti-inflammatory protein tristetraprolin as a hyperphosphorylated protein by mass spectrometry and site-directed mutagenesis. Biochemical Journal, 394:285-297, 2006.
Cao, H. Expression, purification, and biochemical characterization of the antiinflammatory tristetraprolin: a zinc-dependent mRNA binding protein affected by posttranslational modifications. Biochemistry, 43:13724-13738, 2004.
Cao, H., Tuttle, J.S. and Blackshear, P.J. Immunological characterization of tristetraprolin as a low abundance, inducible, stable cytosolic protein. Journal of Biological Chemistry, 279:21489-21499, 2004.
Cao, H., Dzineku, F. and Blackshear, P.J. Expression and purification of recombinant tristetraprolin that can bind to tumor necrosis factor-alpha mRNA and serve as a substrate for mitogen-activated protein kinases. Archives of Biochemistry and Biophysics, 412:106-120, 2003.
Carballo, E., Cao, H., Lai, W.S., Kennington, E.A.,
Cao, H., James, M.G. and Myers, A.M. Purification and characterization of soluble starch synthases from maize endosperm. Archives of Biochemistry and Biophysics, 373:135-146, 2000.
Cao, H. and Preiss, J. Site-directed mutagenesis evidence for arginine-384 residue at the active site of maize branching enzyme II. Journal of Protein Chemistry, 18:379-386, 1999.
Cao, H., Imparl-Radosevich, J., Guan, H., Keeling, P.L., James, M.G. and Myers, A.M. Identification of the soluble starch synthase activity of maize endosperm. Plant Physiology, 120:205-216, 1999.
Beatty, M.K., Rahman, A., Cao, H., Woodman, W., Lee, M., Myers, A.M. and James, M.G. Purification and molecular genetic characterization of ZPU1, a pullulanase-type starch debranching enzyme from maize. Plant Physiology, 119:255-266, 1999.
Cao, H. and
Cao, H. and
Cao, H., Tong, Y. and Nes, W.D. Screening corn (Zea mays) cDNA library with yeast sterol methyltransferase gene results in the isolation of a full-length cDNA encoding elongation factor 1-alpha (Accession No. U76259). Plant Physiology, 113:1463, 1997.
Cao, H. and Preiss, J. Evidence for essential arginine residues at the active sites of maize branching enzymes. Journal of Protein Chemistry, 15:291-304, 1996.
Cao, H. and
Cao, H., Hu, Z., Tan, K. and Jin, Z. DNA homologues in some species and expression patterns in cucumber organs of genes corresponding to cDNAs from carrot somatic embryogenesis cultures. Journal of Plant Physiology, 149:69-72, 1996.
Cao, H., Sullivan, T.D., Boyer, C.D. and
Cao, H. and Chen, S. Brassinosteroid-induced rice lamina joint inclination and its relation to indole-3-acetic acid and ethylene. Plant Growth Regulation, 16:189-196, 1995.
Cao, H. Relationship between ethylene and brassinosteroid-induced epinasty in the second leaf segments of etiolated rice seedlings. Chinese Bulletin of Botany, 9:42-45, 1992.
Cao, H. Comparative study on the effect of brassinosteroid and IAA on rice laminae inclination. Chinese Bulletin of Botany, 8:43-45, 1991.
Cao, H. Two modifications of the rice lamina joint inclination bioassay for brassinosteroid-like substances. Plant Physiology Communications, 27:55, 1991.
Cao, H. and Chen, S. Physiological effects of brassinosteroid isolated from beeswax. Chinese Bulletin of Botany, 6:40-44, 1989.
Cao, H., Chen, S. and Jiang, J. Isolation and biological activity of brassinosteroid-like substance from beeswax. Chinese Journal of Nature, 10:952-953, 1987.
Jiang, J., Chen, S., Liu, G., Pan, X. and Cao, H. Study of plant hormones in beeswax - brassinosteroids. Chinese Science Bulletin, 28:60-63, 1987.
Cao, H., Chen, S. and Pan, X. Effect of sodium bicarbonate concentration on growth and net photosynthetic rate of Spirulina platensis.
Cao, H. Biochemical and molecular characterization of the brittle1 locus in maize and other plants. Dissertation Services, University Microfilms International. Ann Arbor, MI, USA, pp 1-183, 1995.
Cao, H. Biochemical and molecular characterization of the brittle1 locus in maize and other plants.
Cao, H. Preliminary study of the biological effects of brassinosteroid - a plant steroid hormone from beeswax.
Publications (Invited review articles)
Cao, H. Improving recombinant protein purification yield. Chemistry Today, Submitted, 2009
Cao, H., Qin, B., Panickar, P.S. and Anderson, R.A. Tea and cinnamon polyphenols improve the metabolic syndrome. Agro FOOD industry hi-tech, 19: 14-17, 2008.
Cao, H., Deterding, L.J. and Blackshear, P.J. Phosphorylation site analysis of the anti-inflammatory and mRNA destabilizing protein tristetraprolin. Expert Review of Proteomics, 4:711-726, 2007.
Publications (Invited book chapters)
Cao, H., Urban, J.F. Jr. and Anderson, R.A. Cinnamon: Molecular evidence for the health benefits through its insulin-like and anti-inflammatory effects. In Gupta, V.K., Editor, Bioactive Natural Products, Studium Press LLC, USA, in press, 2009.
Cao, H., Roussel, A.M. and Anderson, R.A. Green tea: Molecular targets in inflammation, glucose uptake, and insulin signaling pathways. In Gupta, V.K., Editor, Bioactive Natural Products, Studium Press LLC, USA, in press, 2009.
Panickar, P.S., Cao, H., Qin, B. and Anderson, R.A. Molecular targets and health benefits of cinnamon. In Aggarwal BB and Kunnumakkara AB, Editors, Molecular Targets and Therapeutic Uses of Spices: Modern Uses for Ancient Medicine, World Scientific Publishing Co.,
Cao, H. Tristetraprolin family proteins may prevent and treat cardiovascular diseases. In Takeyama, T., Editor, Messenger RNA Research Perspectives, Nova Science Publishers, Inc., pp 9-16, 2007.
Cao, H. and Lin, R. Growth Factors, Cytokines and Chemokines: Formulations, Delivery, and Pharmacokinetics. In Gad, S.C., Editor-in-Chief, Handbook of Pharmaceutical Biotechnology, John Wiley & Sons, Inc., pp 1197-1224, 2007.
Cao, H. and Lin, R. Expression and purification of the anti-inflammatory tristetraprolin (TTP), an mRNA binding and destabilizing protein. In Ostrovskiy, M.H., Editor, Leading-Edge Messenger RNA Research Communications, Nova Science Publishers, Inc., pp 191-208, 2007.
Cao, H., Chen, S.K. and Jiang, J.X. Isolation and physiological effects of brassinosteroid from beeswax. In Schultz, J.C. and Raskin, I., Editors, Current Topics in Plant Physiology, Vol. 11 - Plant Signals in Interaction with Other Organisms, American Society of Plant Biologists, Rockville, MD, pp 243-247, 1993.
Publications (Invited talks published as PowerPoint presentations on CD-ROM)
Cao, H. Quantitative evaluation of His-tag purification and immunoprecipitation of recombinant proteins. In PepTalk's Protein Purification and Recovery Conference, Cambridge Healthtech Institute. Coronado, San Diego, CA, 2009.
Cao, H. Purification of the anti-inflammatory protein tristetraprolin to homogeneity. In PepTalk's Protein Production - Details for Achieving Success Conference, Cambridge Healthtech Institute. Coronado, San Diego, CA, 2008.
Cao, H. Expression of the mRNA destabilizing protein tristetraprolin and its homologues in bacterial and mammalian cells. In PepTalk's Protein Expression - Optimizing Outcomes for Maximum Efficiency Conference, Cambridge Healthtech Institute. Coronado, San Diego, CA, 2008.
Cao, H. Post-translational modifications of the mRNA destabilizing protein tristetraprolin. In Beyond Genome's Proteomics - Dynamics of Change Conference, Cambridge Healthtech Institute. San Francisco, CA, 2007.
Cao, H. The anti-inflammatory protein tristetraprolin is phosphorylated at multiple sites by multiple protein kinases. In Protein Kinase Targets Conference, Cambridge Healthtech Institute, Boston, MA, 2007.
Cao, H. Production of recombinant antibodies for low-abundance protein. In Protein Engineering Submit's Recombinant Antibodies - From Concept to Clinic Conference, Cambridge Healthtech Institute, Boston, MA, 2006.
Cao, H. Expression and purification of tristetraprolin: an arthritis-suppressor protein. In Protein Engineering Submit's Difficult to Express Proteins Conference, Cambridge Healthtech Institute, Cambridge, MA, 2005.