Page Banner

United States Department of Agriculture

Agricultural Research Service

Mass Spectra of Nematode Sterols
headline bar
Mass Spectra of Nematode Sterols
 
Sterols are important biomolecules within nematodes because sterols function as membrane components and serve as metabolic precursors to steroid hormones.  From the perspective of a nematode, sterols are nutritionally important because nematodes lack the ability to biosynthesize sterols, unlike plants or higher animals.  Therefore, nematodes possess a nutritional requirement for sterols, which parasitic nematodes must obtain from their animal or plant hosts.  This nutritional requirement can be exploited in the design of novel strategies for controlling agriculturally important plant-parasitic nematodes.

Consequently, a large part of David Chitwood's research has focused on the biochemistry of sterols in nematodes.  A large number of structurally diverse sterols have been isolated by us from nematodes or their hosts or their diets; these sterols have been identified via gas chromatography and mass spectrometry.

As a courtesy to the scientific community, we present a table containing mass spectral data of sterols isolated by us from nematodes or their hosts or diets.  This table is based upon a similar table in Lipids, volume 26, pages 619-627, 1991, "Metabolism of plant sterols by nematodes", by D. J. Chitwood and W. R. Lusby.  The U.S. Government has the right to retain a nonexclusive, royalty-free license in and to any copyright covering this paper.


Mass Spectral Data for Nematode Sterols, Analyzed as Steryl Acetate Derivativesa 

Steryl acetateMass spectrum ((m/z, relative intensity to base peak)
Cholesta-5,7,9(11)-trienol 424 (5), 364 (100), 349 (33), 251 (31), 209 (64), 197 (43), 195 (52)
Cholest-8(14)-enol 428 (100), 413 (18), 368 (6), 353 (13), 315 (16), 288 (7), 273 (6), 255 (21), 229 (42), 213 (43), 81 (80), 55 (83)
Cholesterol 368 (100), 353 (14), 260 (15), 255 (13), 247 (18), 213 (14), 147 (48), 145 (37), 81 (74), 55 (71) 
Cholestanol430 (12), 415 (2), 370 (33), 355 (16), 316 (3), 276 (28), 275 (18), 257 (4), 230 (17), 215 (100), 201 (18), 147 (32), 81 (53)
Cholest-8(9)-enol428 (89), 413 (18), 368 (5), 353 (16), 315 (9), 255 (23), 229 (37), 213 (46), 55 (100). 
Cholesta-7,9(11)-dienol426 (100), 411 (22), 366 (19), 351 (37), 313 (30), 286 (19), 271 (11), 259 (31), 253 (38), 211 (62), 199 (33),  55 (50) 
Cholesta-5,7,9(11),24-tetraenol 422 (4), 362 (63), 347 (15), 251 (18), 249 (14), 209 (47), 195 (39), 69 (100)
Desmosterol 366 (51), 351 (11), 253 (23), 213 (10), 145 (24), 69 (100) 
7-Dehydrocholesterol 426 (2), 366 (100), 351 (34), 253 (23), 211 (17), 199 (20), 158 (62), 143 (86), 55 (93) 
24-Methylcholesta-5,22(E)-dienol380 (100), 365 (5), 337 (3), 282 (7), 255 (49), 213 (11), 81 (78), 69 (82) 
Cholest-24-enol428 (4), 413 (8), 353 (4), 344 (12), 317 (3), 315 (70), 257 (6), 255 (43), 229 (3), 215 (17), 69 (100) 
Lathosterol 428 (94), 413 (17), 368 (6), 353 (13), 315 (12), 288 (6), 273 (10), 255 (89), 229 (31), 213 (53), 81 (93), 55 (100) 
24-Methylcholest-22(E)-enol442 (18), 344 (29), 329 (3), 315 (36), 257 (57), 215 (14), 55 (100) 
Cholesta-8(9),24-dienol 426 (23), 411 (10), 351 (8), 315 (1), 313 (3), 255 (3), 229 (11), 213 (32), 69 (100), 55 (51) 
Cholesta-5,7,9(11),22(E),24-pentaenol420 (1), 360 (14), 345 (1), 251 (14), 249 (7), 109 (100) 
Cholesta-5,22(E),24-trienol424 (1), 364 (1), 313 (8), 282 (24), 255 (6), 253 (16), 213 (2), 199 (1), 109 (100) 
Cholesta-5,7,24-trienol424 (3), 364 (98), 349 (20), 253 (16), 251 (10), 211 (15), 199 (13), 69 (100) 
Cholesta-7,24-dienol426 (11), 411 (11), 351 (4), 315 (3), 313 (100), 255 (12), 229 (7), 213 (23), 69 (98), 55 (54) 
24-Methylcholesta-5,23-dienol 380 (28), 365 (3), 296 (3), 283 (34), 259 (4), 255 (6), 253 (24), 227 (4), 215 (6), 213 (8), 159 (27), 145 (37), 133 (54), 55 (100)
24-Methylcholesta-5,7,9(11)-trienol 438(5), 378 (100), 363 (23), 251 (27), 225 (13), 209 (64), 195 (53), 158 (14), 143 (19), 55 (99) 
24-Methylenecholesterol 380 (57), 365 (5), 296 (32), 281 (9), 253 (17), 213 (13), 81 (87), 55 (100) 
Cholesta-5,7,22(E),24-tetraenol422 (1), 362 (26), 347 (3), 280 (1), 253 (10), 251 (6), 157 (27), 143 (22), 109 (100) 
24-Methylenecholestanol442 (1), 427 (1), 358 (46), 343 (11), 315 (21), 298 (2), 283 (4), 255 (11), 215 (23), 55 (100) 
24-Methylcholesterol382 (100), 367 (14), 274 (15), 261 (12), 255 (17), 213 (16), 147 (63), 81 (60)
24-Methylcholestanol444 (17), 429 (2), 384 (31), 369 (16), 330 (2), 317 (1), 315 (1), 276 (33), 275 (21), 257 (6), 230 (14), 215 (100), 201 (19), 147 (34), 81 (53) 
24-Methylenecholesta-5,7-dienol438 (1), 378 (45), 363 (6), 253 (8), 227 (1), 211 (5), 158 (30), 143 (29), 55 (100) 
24-Ethylcholesta-5,22(E)-dienol394 (92), 379 (3), 351 (6), 313 (2), 282 (6), 255 (35), 228 (4), 213 (6), 81 (87), 55 (100) 
24-Methylcholesta-7,24(28)-dienol 440 (3), 425 (3), 365 (2), 356 (22), 315 (2), 313 (100), 255 (14), 253 (9), 227 (12), 213 (26) 
24-Methylcholesta-5,7-dienol440 (1), 380 (100), 365 (14), 313 (1), 253 (37), 211 (18), 199 (19), 158 (81), 143 (79) 
24-Ethylcholest-22(E)-enol456 (9), 353 (9), 344 (14), 329 (2), 317 (2), 315 (19), 257 (36), 255 (8), 215 (5), 55 (100) 
24-Methylcholest-7-enol442 (40), 427 (6), 382 (3), 367 (5), 315 (7), 255 (100), 229 (29), 213 (52), 81 (67) 
24-Methylcholesta-5,24(25)-dienol380 (27), 365 (5), 296 (53), 281 (17), 253 (19), 213 (17), 83 (100) 
24-Ethylcholesta-5,22(E),24(28)-trienol 452 (1), 392 (12), 313 (18), 282 (89), 255 (24), 253 (78), 213 (8), 157 (14), 143 (14), 137 (48), 95 (100)
24-Ethylcholesta-5,23-dienol394 (100), 379 (7), 283 (26), 253 (22), 213 (11), 81 (78), 55 (96)
24-Ethylcholesta-7,22(E)-dienol454 (21), 439 (4), 411 (7), 394 (4), 379 (4), 351 (6), 342 (13), 315 (13), 313 (86), 255 (37), 229 (14), 213 (15), 81 (93), 55 (100)
Fucosterol394 (23), 379 (2), 296 (100), 281 (14), 253 (8), 228 (6),  213 (13), 55 (92)
24-Ethylcholesterol396 (100), 381 (14), 288 (13), 275 (13), 255 (19), 213 (17), 147 (52), 81 (56)
24-Ethylcholestanol458 (18), 443 (2), 398 (32), 383 (13), 344 (3), 276 (23), 275 (21), 257 (6), 230 (15), 215 (100), 201 (17), 147 (34), 81 (49) 
Isofucosterol 394 (12), 379 (1), 296 (100), 281 (19), 253 (8), 228 (7), 213 (11), 55 (87) 
Isofucostanol456 (2), 358 (83), 343 (15), 315 (7), 298 (9), 283 (10), 255 (8), 215 (24), 55 (100) 
24-Ethylcholesta-5,24(25)-dienol 394 (32), 379 (6), 296 (100), 281 (23), 253 (18), 228 (8), 213 (9), 55 (81) 
24-Ethylcholesta-5,7-dienol454 (1), 394 (100), 379 (21), 253 (40), 211 (22), 199 (23), 158 (84), 143 (87)
24-Ethyllathosterol456 (24), 441 (1), 396 (1), 381 (1), 315 (3), 255 (73), 229 (20), 213 (42), 81 (97), 55 (100) 
24-Ethylcholest-24(25)-enol456 (79), 441 (6), 358 (52), 343 (13), 315 (19), 283 (6), 255 (48), 229 (16), 213 (23), 55 (100)
24Z-Ethylidenelathosterol454 (2), 439 (1), 394 (1), 356 (37), 313 (100), 296 (4), 281 (5), 255 (5), 253 (4), 213 (12), 55 (48) 
4a-Methylcholest-8(14)-enol442 (36), 427 (4), 382 (2), 367 (7), 329(4), 269 (14), 243 (23), 227 (34), 95 (73), 55 (100) 
4a-Methylcholestanol444 (17), 429 (4), 384 (48), 369 (30), 355 (7), 343 (4), 329 (1), 316 (1), 290 (26), 289 (17), 262 (13), 244 (21), 229 (100), 215 (23), 95 (74), 55 (59)
4a-Methylcholesta-8(14),24-dienol440 (19), 425 (6), 365 (5), 329 (1), 327 (2), 269 (2), 243 (9), 227 (16), 69 (100), 55 (57) 
4a-Methylcholest-7-enol442 (33), 427 (4), 382 (4), 367 (6), 329(4), 269 (52), 243 (18), 227 (36), 95 (96), 55 (100)
4a-Methylcholesta-7,24-dienol440 (1), 425 (3), 365 (1), 329 (1), 327 (67), 269 (4), 243 (2), 227 (10), 69 (100), 55 (68) 
Obtusifoliol 468 (28), 453 (64), 393 (22), 369 (9), 343 (4), 309 (11), 287 (19), 227 (14), 55 (100) 
4a,24-Dimethylcholest-8(14)-enol456 (20), 441 (2), 396 (1), 381 (2), 329 (3), 269 (9), 243 (15), 227 (23), 95 (64), 55 (100) 
4a,24-Dimethylcholestanol458 (18), 443 (3), 398 (48), 383 (31), 369 (7), 290 (32), 289 (18), 271 (6), 244 (23), 230 (57), 229 (100), 215 (22) 
4a,24-Dimethylcholest-7-enol456 (26), 441 (2), 396 (2), 381 (3), 329 (4), 269 (53), 243 (14), 227 (33), 95 (93), 55 (100) 
4a-Methyl-24-ethylcholest-22(E)-enol470 (28), 455 (2), 427 (2), 367 (17), 358 (24), 343 (4), 329 (23), 271 (33), 229 (8), 55 (100) 
4a-Methyl-24-methylenelathosterol454 (6), 439 (5), 394 (4), 379 (3), 370 (22), 327 (100), 269 (12), 241 (23), 227 (20) 
4a-Methyl-24-ethylcholestanol472 (16), 457 (3), 412 (47), 397 (24), 383 (7), 290 (32), 289 (10), 271 (7), 244 (21), 230 (56), 229 (100), 215 (23)
4a-Methylisofucostanol470 (4), 455 (1), 372 (100), 357 (12), 329 (6), 312 (4), 269 (3), 230 (27), 229 (18)
4a-Methyl-24(Z)-ethylidenelathosterol 468 (12), 453 (9), 408 (6), 370 (33), 327 (100), 267 (5), 241 (9), 227 (7), 55 (65) 
Cycloartanol 470 (1), 455 (2), 410 (8), 395 (9), 367 (3), 357 (1), 355 (1), 341 (3), 297 (6), 288 (10), 273 (3), 55 (100) 
Cycloeucalenol468 (4), 453 (7), 408 (62), 393 (37), 300 (6), 283 (8), 281 (7), 55 (100)
Cycloartenol468 (1), 453 (1), 408 (11), 393 (7), 365 (3), 339 (2), 297 (1), 295 (1), 286 (7), 271 (4), 257 (1), 255 (1), 243 (2), 69 (100) 
24-Methylenecycloartanol482 (1), 467 (1), 422 (9), 407 (6), 379 (4), 353 (1), 300 (4), 297 (2), 285 (1), 55 (100) 

a Numbers in parentheses are relative abundance (%). 
Electron impact spectra were obtained in a Finnigan 4510 mass spectrometer equipped with a 15-meter x 0.32-mm DB-1 capillary column and connected to an Incos data system.  Mention of trade names or commercial products in this table is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. 

 
Sterol retention times during gas chromatography
 
 
 


Last Modified: 8/13/2016
Footer Content Back to Top of Page