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Shin-Yi Marzano

Research Molecular Biologist


 

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Shin-Yi Lee Marzano, Ph.D.
Research Molecular Biologist
Greenhouse Production Research Group
USDA-ARS Application Technology Research Unit
2801 W. Bancroft St., Mail Stop 604, 4271B Wolfe Hall
Toledo OH 43606
Office (419) 530-5053

I have been interested in beneficial microbes that are useful in managing diseases, whether in plant health or human health. I am motivated to study microbiomes and viromes because antimicrobial resistance is a substantial cause of death by infectious disease in the United States, and anti-fungicidal resistance is a big concern in agriculture. With a training in Environmental Microbiology, I am also interested in manipulating microbial pathways that can eliminate nutrient loss in runoff.

As part of my postdoc work, I surveyed the fungal virome in Sclerotinia sclerotiorum (white mold). The genome of S. sclerotiorum has been sequenced and is suitable as a model system from which the most diverse lineages of mycoviruses have been discovered. These mycoviruses include positive- and negative-stranded ssRNA, dsRNA viruses and a ssDNA virus capable of extracellular transmission. My current lab and the others have found that disruption mutants of S. sclerotiorum genes are reasonably easy to obtain. Therefore, as a continuation of a previous genome-wide investigation, my lab is dissecting the RNA silencing pathways of S. sclerotiorum, validating a short list of fungal genes targeted by small RNA-mediated degradation, either induced by mycovirus infection causing hypovirulence (reduced virulence) or by endogenous small RNAs. We approach this line of investigation by establishing reverse genetic systems to look at the effect of virus suppressor of gene silencing (VSR), the effect of different viral infections by synthetic biology, and aiming to optimize the viral systems to deliver transgenes to induce gene silencing (VIGS). Building on what we’ve learned in the past years, we are also interested in learning how secondary small RNAs are produced, and what biological significance they have in fungi.

Shin-Yi Marzano Google Scholar 

 

Educational Background
PhD, Crop Sciences, University of Illinois, Urbana-Champaign, USA
MSc, Environmental Engineering, University of Illinois, Urbana-Champaign, USA
MSc, Environmental Microbiology, Aberdeen University, UK
BSc, Environmental Health, National Taiwan University