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Key Publications
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••         Naturally occurring microbes and enzymes for biobased products


S. Liu, B. Saha, and M. Cotta. 2005. Cloning, expression, purification, and analysis of mannitol dehydrogenase gene mtlK from Lactobacillus brevis. J. Appl. Biochem. & Biotechnol. 121-124: 391-402.


Bischoff, K.M., Liu, S., and Hughes, S.R. 2007. Cloning and characterization of a recombinant family 5 endoglucanase from Bacillus licheniformis strain B-41361.  Process Biochem. 42:1150-1154


Liu, S., Skinner-Nemec, K.A., and Leathers, T.D. 2008. A Lactobacillus buchneri strain NRRL B-30929 converts a mixture of xylose and glucose into ethanol and other products.  J. Ind Microbiol Biotechnol. 35:75-81


Liu, S., and Cotta, M.A. 2008. Gram-positive bacteria as biocatalysts to convert biomass derived sugars into biofuel and chemicals. In: Hou, C.T., and Shaw, J.-F. (eds). Book chapter for Biocatalysis and Bioenergy, John Wiley & Sons, Ltd., p. 249-264


Liu, S., Bischoff, K.M., Hughes, S.R., Leathers, T.D., Price, N.P., Qureshi, N., and Rich, J.O. 2009. Conversion of biomass hydrolysates and other substrates to ethanol and other chemicals by Lactobacillus buchneri.  Lett. Appl. Microbiol. 48: 337-342.


K. M. Bischoff, S. Liu, T.D. Leathers, R.E. Worthington and J.O. Rich. 2009. Modeling bacterial contamination of fuel ethanol fermentation.   Biotechnology and Bioengineering 103: 117-122.


N. Qureshi, B.C. Saha, R.E. Hector, B.S. Dien, S.R. Hughes, S. Liu, L.B. Iten, M.J. Bowman, G. Sarath, and M.A. Cotta. 2010. Production of a superior biofuel "butanol from agricultural residues: part II-use of corn stover and switchgrass hydrolyzates.  Biomass and Bioenergy. 34: 566-571.


S. Liu, Bischoff, Leathers, Qureshi, Rich, and Hughes. 2013 Butyric acid from anaerobic fermentation of lignocellulosic biomass hydrolysates by Clostridium tyrobutyricum strain RPT 4213.  Bioresource Technology 143 (2013) 322-329


Qureshi N, Singh V., Liu S., Ezeji T.C., Saha B.C., Cotta M.A. 2014 Process integration for simultaneous saccharification, fermentation, and recovery (SSFR): Production of butanol from corn stover using Clostridium beijerinckii P260. Bioresource Technology 154:222-228


••         Environmental stress induced gene expression in Gram-positive bacteria


Liu, S., Graham, J.E., Bigelow, L., Morse II, P.D., and Wilkinson, B.J. 2002. Identification of Listeria monocytogenes genes expressed in response to growth at low temperature.  Appl. Environ. Microbiol. 68:1697-1705


Liu, S., Bayles, D., Mason, T.M., and Wilkinson, B.J. 2006. A cold-sensitive Listeria monocytogenes mutant has a transposon insertion in a gene encoding a putative membrane protein, and shows altered (p)ppGpp levels.  Appl. Environ. Microbiol. 72:3955-3959


S. Liu and N. Qureshi. 2009. How microbes tolerate ethanol and butanol. New Biotechnol. 26: 117-121.  This article was ranked as top cited article for 2009-2011 period by the publisher (Elsevier)


S. Liu, K.M. Bischoff, T.D. Leathers, N. Qureshi, J.O. Rich and S. R. Hughes. 2012. Adaptation of lactic acid bacteria to butanol.  Biocatalysis and Agricultural Biotechnology.  1: 57-61.


S. Liu. 2014 Proteomic analyses of ethanol tolerance in Lactobacillus buchneri NRRL B-30929  Submitted to Proteomics


••         Genetic modifications of microbial biocatalysts for biofuels and bioproducts


Liu, S., Dien, B., and Cotta, M. 2005. Functional expression of bacterial Zymobacter palmae pyruvate decarboxylase gene in lactic acid bacteria.  Curr. Microbiol. 50:1-6


Liu, S., Saha, B., and Cotta, M. 2005. Cloning, expression, purification, and analysis of mannitol dehydrogenase gene mtlK from Lactobacillus brevis.  J. Appl. Biochem. Biotechnol. 121-124:391-402


Liu, S., Nichols, N., Dien, B., and Cotta, M. 2006. Metabolic engineering of a Lactobacillus plantarum double ldh knockout strain for enhanced ethanol production.  J. Ind. Microbiol. Biotechnol. 33:1-7


Liu. S. 2006. A simple method to generate chromosomal mutations in Lactobacillus plantarum strain TF103 to eliminate undesired fermentation products.  J. Appl. Biochem. Biotechnol. 129-132:854-863


Liu, S., Dien, B.S., Nichols, N.N., Bischoff, K.M., Hughes, S.R., and Cotta, M.A. 2007. The production of recombinant pyruvate decarboxylase and alcohol dehydrogenase in Lactobacillus brevis ATCC 367.  FEMS Microbial. Letters. 274:291-297


Hughes,S. R., P. F. Dowd, R. Hector, S. B. Riedmuller, S Bartolett, J. A. Mertens, N. Qureshi, S. Liu, K. M. Bischoff, X.L. Li, J. S. Jackson, Jr., W. A. Chau, D. Sterner, T.Panavas, M. A. Cotta, P. J. Farrelly, and T. Butt. 2007. Fully automated molecular biology protocols on a plasmid-based functional proteomic robotic workcell used for high-throughput multiplex preparation of a library of synthetic mutagenized insecticidal peptides.  J. Assoc. Lab. Autom. 12: 202-212.


Qureshi N, Dien BS, Liu S, Saha BC, Cotta MA, Hughes S, Hector R. 2012. Genetically engineered Escherichia coli FBR5: Part II. Ethanol production from xylose and simultaneous product recovery. Biotechnol Prog. 2012 Jun 26. doi: 10.1002/btpr.1584.


Hughes, Moser, Robinson, Cox, Harmsen, Friesen, Bischoff, Jones, Pinkleman, Bang, Tasaki, Doll, Qureshi, S. Liu, Saha, Jackson, Cotta, Rich, and Caimi 2012.  Synthetic resin-bound truncated Candida antarctica lipase B for production of fatty acid alkyl esters by transesterification of corn and soybean oils with ethanol or butanol. J Biotechnol. 159:69-77.


••         Recombinant DNA technology and gene regulation in Eukaryotes 7


S. Liu and A. Kriz. 1996. Tissue-specific and ABA-regulated maize Glb1 gene expression in transgenic tobacco.  Plant Cell Report. 15: 158-162.


H. Li, S. Liu, and B. Kemper. 1996. Sex- and tissue-specific expression of a cytochrome P450 2C2-luciferase transgene.  Molecular and Cellular Endocrinology. 120: 77-83.S.


S. Liu, D. Duncan, A. Kriz and J. Widholm. 1998. Abscisic acid-regulated Glb1 transient expression in cultured maize P3377 cells.  Plant Cell Report. 17: 646-649.


Liu,Y. Park, I. Rivera-Rivera, H. Li, and B. Kemper. 1998. Nuclear factor-1 motif redundant regulatory elements comprise a phenobarbital responsive enhancer.  DNA and Cell Biology. 17: 461-470.


S. Liu and J. Cowell. 2000. Cloning and characterization of the TATA-less promoter from human proto-oncogene GFI1.  Annals of Human Genetics. 64: 83-86.


R.P. Somerville, O. Chernova, S. Liu, Y. Shoshan, and J.K. Cowell. 2000. Identification of the promoter, genomic structure, and mouse ortholog of LGI1.  Mamm Genome. 11: 622-627.


S. Liu, I. Rivera-Rivera, A.J. Bredemeyer and B. Kemper. 2001.  Functional analysis of the phenobarbital-responsive unit in rat CYP2B2. Biochem. Pharmacol. 62: 21-28.