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1 - Germination Test Methods
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Click here for information on the seed testing papers we use.
Contact Curator Laura Marek.
Alliaria 493335 Erysimum 493335 Alyssum 493339 Euphorbia 492112 Asters 491490 Goldbachia 493220 Berteroa 493339 Helianthus (cultivated) 105 Biscutella 490360 Helianthus (wilds) 131 Brassica carinata 178 Hesperis 493335 Brassica juncea 181 Iberis
Brassica napus 178 Isatis
Brassica nigra 185 Lepidium
Brassica rapa 178 Linum (cultivated)
Brassica repanda 493043 Linum (wilds)
Brassica tournefortii 181 Linum flavum 490360 Camelina 493220 Linum lewisii 493622 Crambe 493339 Linum tenuifolium 493809 Cuphea 493034 Matthiola 493336 Eruca 493339 Sinapis 493335 Erucastrum 493339 Thlaspi 493336
ENO: 493335 Alliaria, Erysimum, Hesperis, Sinapis
Duration
Temperature
Fluorescent Lights
7 days 4 ?C --- 7 days 12 hours 30 ?C
ON
12 hours 20 ?C
OFF
14 days + 4 ?C --- 7 days 12 hours 30 ?C ON 12 hours 20 ?C OFF Seeds are placed on top of 2 blue blotter papers moistened with tap water in plastic boxes (5" x 5-1/4"); let sit overnight at room temperature. Prechill at 4 ?C for 7 days. Transfer to germinator with temperatures alternating between 30 ?C with light for 12 hours and 20 ?C in darkness for 12 hours per 24 hour cycle. Leave at this regimen for 7 days. Place germination boxes back in cold room for at least 14 days - leave until seeds begin to germinate in cold room. Transfer back to 20/30 ?C, as before. Score immediately and at 7, 14, and 21 days. Replication and sample size: 4 reps of 50 seed each for a total of 200 seeds.
ENO: 493339 Alyssum, Berteroa, Crambe,
Eruca, Erucastrum, IsatisDuration
Temperature
Fluorescent Lights
7 days
4 ?C
---
12 hours
20 ?C
ON
12 hours
20 ?C
OFF
Seeds are placed between 2 blue blotter papers moistened with 0.1% KNO3 solution in plastic boxes (5" x 5-1/4") and kept at room temperature overnight. Prechill at 4 ?C for 7 days. Transfer to germinator set at constant 20 ?C with light for 12 hours and dark for 12 hours per 24 hour cycle. Replication and sample size: 4 reps of 50 seed each for a total of 200 seeds. Germination counts occur weekly after start of test.
ENO: 491490 Asters
Duration
Temperature
Fluorescent Lights
14 days
4?C
---
16 hours
25 ?C
ON
8 hours
15?C
OFF
Seeds are placed on top of 2 blue blotter papers moistened with 1 mM GA3 in plastic boxes (5" x 5-1/4"); let sit overnight at room temperature. Prechill at 4 ?C for 14 days. Transfer to germinator with temperatures alternating alternating between 25 ?C with light for 16 hours and 15 ?C in darkness for 8 hours per 24 hour cycle. Replication and sample size: 4 reps of 50 seed each for a total of 200 seeds. Germination counts occur at 7, 14, and 21 days after start of the germination test.
Additional comments: Madia prefers 15 ?C constant and dark environment.
ENO: 490360 Biscutella, Linum flavum
Duration
Temperature
Fluorescent Lights
12 hours
30 ?C
ON
12 hours
20?C
OFF
Seeds are placed on top of 3 blotter papers (1 blue, 2 white - in contact with seeds) moistened with 0.1% KNO3 in plastic boxes (5" x 5-1/4"); let sit overnight at room temperature. Transfer to germinator with temperatures alternating alternating between 30 ?C with light for 12 hours and 20 ?C in darkness for 12 hours per 24 hour cycle. Replication and sample size: 4 reps of 50 seed each for a total of 200 seeds. Germination counts occur at 7, 14, and 21 days after start of test.
ENO: 178 Brassica (carinata, napus, rapa)
Duration
Temperature
Fluorescent Lights
12 hours
25 ?C
ON
12 hours
15?C
OFF
Seeds are placed between blotter papers moistened with 0.1% KNO3 in plastic boxes (5" x 5-1/4") with the following arrangement:
(top) 1 blue paper
seeds
1 white paper
(bottom) 1 blue paperLet seeds imbibe overnight at room temperature; prechill at 4 ?C for 14 days. Transfer to germinator with temperatures alternating alternating between 25 ?C with light for 12 hours and 15 ?C in darkness for 12 hours per 24 hour cycle. Replication and sample size: 4 reps of 50 seed each for a total of 200 seeds. (Alternatively, 2 reps of 100 seeds.) The first germination count occurs between 3 and 7 days and the last count is on the 14th day after start of test.
ENO: 181 Brassica (juncea, tournefortii)
Duration
Temperature
Fluorescent Lights
7 days
10 ?C
---
12 hours
25 ?C
ON
12 hours
15 ?C
OFF
Seeds are placed on top of 2 blue blotter papers moistened with 0.1% KNO3 solution in plastic boxes (5" x 5-1/4") and kept at room temperature overnight. Prechill at 10 ?C for 7 days. Transfer to germinator with temperatures alternating alternating between 25 ?C with light for 12 hours and 15 ?C in darkness for 12 hours per 24 hour cycle. Replication and sample size: 4 reps of 50 seed each for a total of 200 seeds. The first germination count occurs between 3 and 7 days and the last count is on the 14th day after start of the germination test.
ENO: 181 Brassica nigra
Duration
Temperature
Fluorescent Lights
3 days
10 ?C
---
12 hours
25 ?C
ON
12 hours
15 ?C
OFF
Seeds are placed on top of 2 blue blotter papers moistened with 0.1% KNO3 solution in plastic boxes (5" x 5-1/4") and kept at room temperature overnight. Prechill at 10 ?C for 3 days. Transfer to germinator with temperatures alternating alternating between 25 ?C with light for 12 hours and 15 ?C in darkness for 12 hours per 24 hour cycle. Replication and sample size: 4 reps of 50 seed each for a total of 200 seeds. The first germination count occurs between 3 and 7 days and the last count is on the 14th day after start of the germination test.
ENO: 493043 Brassica repanda
Duration
Temperature
Fluorescent Lights
12 hours
30 ?C
ON
12 hours
20?C
OFF
Seeds are placed on top of 3 blotter papers (2 blue, 1 white - in contact with seeds) moistened with 0.1% KNO3 in plastic boxes (5" x 5-1/4") and kept at room temperature overnight. Transfer to germinator with temperatures alternatingalternating between 30 ?C with light for 12 hours and 20 ?C in darkness for 12 hours per 24 hour cycle. Replication and sample size:4 reps of 50 seed each for a total of 200 seeds. Germination counts occur at 7, 14, and 21 days after start of test.
ENO: 493220 Camelina, Goldbachia
Duration
Temperature
Fluorescent Lights
14 days
4 ?C
---
12 hours
25 ?C
ON
12 hours
15 ?C
OFF
Seeds are placed between blotter papers moistened with 0.1% KNO3 in plastic boxes (5" x 5-1/4") with the following arrangement:
(top) 1 blue paper
seeds
1 white paper
(bottom) 1 blue paperLet seeds imbibe overnight at room temperature; prechill at 4 ?C for 14 days. Transfer to germinator with temperatures alternating alternating between 25 ?C with light for 12 hours and 15 ?C in darkness for 12 hours per 24 hour cycle. Replication and sample size: 4 reps of 50 seed each for a total of 200 seeds. One germination count occurs 14 days after start of test; no other counts required.
ENO: 493034 Cuphea
Duration
Temperature
Fluorescent Lights
12 hours
25 ?C
ON
12 hours
15?C
OFF
Seeds are placed on top of 2 blue blotter papers moistened with tap water in plastic boxes (5" x 5-1/4"); let sit for 48 hours at room temperature. Transfer to germinator with temperatures alternatingalternating between 25 ?C with light for 12 hours and 15 ?C in darkness for 12 hours per 24 hour cycle.
Rep and sample size depends on quantity of seed available:
If > 2500 sds, then 4 reps of 50 sd for total of 200 seeds.
If > 1500 & < 2500 , then 4 reps of 25 sd for total of 100.
If < 1500, then do not run germination test.Germination counts occur at 7, 14, and 21 days after start of test.
ENO: 492112 Euphorbia
Duration
Temperature
Fluorescent Lights
14 days
4?C
---
12 hours
30 ?C
ON
12 hours
20?C
OFF
Seeds are placed on top of 2 blue blotter papers moistened with tap water in plastic boxes (5" x 5-1/4"); let sit overnight hours at room temperature. Prechill at 4 ?C for 14 days. Transfer to germinator with temperatures alternatingalternating between 30 ?C with light for 12 hours and 20 ?C in darkness for 12 hours per 24 hour cycle. Replication and sample size:4 reps of 50 seed each for a total of 200 seeds. Germination counts occur at 6 and 16 days after start of test.
ENO: 105 Helianthus (cultivated)
Duration
Temperature
Fluorescent Lights
12 hours
30 ?C
ON
12 hours
20?C
OFF
Seeds are placed in folded paper towels1 moistened with tap water and placed in plastic tubs. Transfer to germinator with temperatures alternating between 30 ?C with light for 12 hours and 20 ?C in darkness for 12 hours per 24 hour cycle.
Rep and sample size depends on quantity of seed available:
If > 1500 sds, then 4 reps of 50 sd for total of 200 seeds.
If > 1200 & < 1500 , then 4 reps of 25 sd for total of 100.
If < 1200, then do not run germination test.Germination counts occur at 5 and 14 days after start of test.
1Seeds are wrapped in 2 sheets of germination toweling and replicated four times. The four replications are rubber banded together and eight accessions are placed in plastic tubs. Towels are kept moist with tap water during the germination period.
ENO: 131 Helianthus (wilds)
Duration
Temperature
Fluorescent Lights
14 days
4?C
---
12 hours
25 ?C
ON
12 hours
15 ?C
OFF
Seeds are placed on top of 2 blue blotter papers moistened with distilled water in plastic boxes (5" x 5-1/4"). Prechill for 14 days. Transfer to germinator with temperatures alternating between 25 ?C with light for 12 hours and 15 ?C in darkness for 12 hours per 24 hour cycle.
Rep and sample size depends on quantity of seed available:
If > 2000 sds, then 4 reps of 50 sd for total of 200 seeds.
If > 1500 & < 2000 , then 4 reps of 25 sd for total of 100.
If < 1500, then do not run germination test.Germination counts occur at 5 and 14 days after start of the germination test.
ENO: 492632 Iberis
Duration
Temperature
Fluorescent Lights
14 days
4?C
---
12 hours
25 ?C
ON
12 hours
15?C
OFF
Seeds are placed on top of 2 blue blotter papers moistened with 0.1% KNO3 in plastic boxes (5" x 5-1/4") and kept at room temperature for 48 hours. Prechill at 4 ?C for 14 days. Transfer to germinator with temperatures alternatingalternating between 25 ?C with light for 12 hours and 15 ?C in darkness for 12 hours per 24 hour cycle. Replication and sample size:4 reps of 50 seed each for a total of 200 seeds. Germination counts occur at 7, 14, and 21 days after start of germination test.
ENO: 493337 Lepidium
Duration
Temperature
Fluorescent Lights
7 days
4 ?C
---
12 hours
15 ?C
ON
12 hours
15 ?C
OFF
Seeds are placed between 2 blue blotter papers moistened with 0.1% KNO3 solution in plastic boxes (5" x 5-1/4") and kept at room temperature overnight. Prechill at 4 ?C for 7 days. Transfer to germinator set at constant 15 ?C with light for 12 hours and dark for 12 hours per 24 hour cycle. Replication and sample size: 4 reps of 50 seed each for a total of 200 seeds. Germination counts occur weekly after start of test.
ENO: 490309 Linum (cultivated)
Duration
Temperature
Fluorescent Lights
12 hours
30 ?C
ON
12 hours
20 ?C
OFF
Seeds are placed on top of 2 blue papers moistened with tap water in plastic boxes (5" x 5-1/4"); let sit overnight at room temperature. Transfer to germinator with temperatures alternatingalternating between 30 ?C with light for 12 hours and 20 ?C in darkness for 12 hours per 24 hour cycle.
Rep and sample size depends on quantity of seed available:
If > 6000 sds, then 4 reps of 50 sd for total of 200 seeds.
If > 2100 & < 6000 , then 4 reps of 25 sd for total of 100.
If < 2100, then do not run germination test.Germination count days also vary:
If L. usitatissimum,
then counts occur on 3 and 7 days after start of test.
Otherwise,
counts occur at 7, 10, and 14 days after start of test.
ENO: 491459 Linum (wilds)
Duration
Temperature
Fluorescent Lights
12 hours
15 ?C
ON
12 hours
15 ?C
OFF
Seeds are placed on top of 2 blue papers moistened with 0.1% KNO3 in plastic boxes (5" x 5-1/4"); let sit overnight at room temperature. Transfer to germinator set at constant temperature of 15 ?C with light for 12 hours and darkness for 12 hours per 24 hour cycle. Replication and sample size: 4 reps of 50 sd for total of 200 seeds.Germination counts occur at 7, 14, and 21 days after start of test.
If seeds do not germinate after 10 days, raise germination temperature to 20 ?C.
ENO: 493622 Linum lewisii
Duration
Temperature
Fluorescent Lights
28 days
4 ?C
---
12 hours
15 ?C
ON
12 hours
15 ?C
OFF
Seeds are placed on top of 2 blue papers moistened with tap water in plastic boxes (5" x 5-1/4"); let sit overnight at room temperature. Prechill at 4 ?C for 28 days. Transfer to germinator set at constant temperature of 15 ?C with light for 12 hours and darkness for 12 hours per 24 hour cycle. Replication and sample size: 4 reps of 50 sd for total of 200 seeds.Germination counts occur at 7, 14, and 21 days after start of germiantion test.
If seeds do not germinate after 10 days, raise germination temperature to 20 ?C.
ENO: 493809 Linum tenuifolium
Duration
Temperature
Fluorescent Lights
21 days
4 ?C
---
12 hours
25 ?C
ON
12 hours
15 ?C
OFF
Seeds are placed on top of 2 blue papers moistened with tap water in plastic boxes (5" x 5-1/4"); let sit overnight at room temperature. Prechill at 4 ?C for 21 days. Transfer to germinator with temperatures alternatingalternating between 25 ?C with light for 12 hours and 15 ?C in darkness for 12 hours per 24 hour cycle.Replication and sample size: 4 reps of 50 sd for total of 200 seeds.Germination counts occur at 7, 14, and 21 days after start of germination test.
ENO: 493336 Matthiola, Thlaspi
Duration
Temperature
Fluorescent Lights
7 days
4 ?C
---
12 hours
30 ?C
ON
12 hours
20?C
OFF
Seeds are placed on top of 2 blue blotter papers moistened with 0.1% KNO3 in plastic boxes (5" x 5-1/4"); let sit overnight at room temperature. Prechill at 4 ?C for 7 days. Transfer to germinator with temperatures alternating between 30 ?C with light for 12 hours and 20 ?C in darkness for 12 hours per 24 hour cycle. Replication and sample size:4 reps of 50 seed each for a total of 200 seeds. Germination counts occur at 7, 14, and 21 days after start of germination test.
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