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1 - Germination Test Methods
2 - Page 7
3 - Page 8
4 - Page 2
5 - Page 4
6 - Page 5
7 - Page 1
8 - Page 6

Click here for information on the seed testing papers we use.
Contact Curator David Brenner.

Amaranth
491828
Ferula
493851
Ammi
493850
Foeniculum
493814
Anethum
493834
Galega
493836
Angelica
493921
Grasses
490363
Anthriscus
493835
Levisticum
493851
Astrodaucus
490972
Melilotus
492586
Bifora
493836
Oreoschimperella
493840
Calamovilfa
152
Orlaya
493850
Caucalis
490972
Panicum
490363
Celosia
491462
Perilla
493899
Chenopodium
(cultivated)
491651
Petroselinum
493801
Chenopium
(wild)
493487
Setaria
(cultivated)
490361
Coniocelinum
493840
Setaria
(wild)
490385
Coriander
492498
Sium
493852
Cuminum
491828
Spinacia
491601
Dalea
493836
Umbels
490972
Echinochloa
(standard) 490362 Zizia 490972
Echinochloa
(side-by-side) 494560
Eryngium
491625

ENO: 491828 Amaranth & Cuminum
Duration
Temperature
Fluorescent Lights
12 hours
30 ?C
ON
12 hours
20 ?C
OFF
Seeds are placed on top of 2 blue blotter papers moistened with tap water in plastic boxes (5" x 5-1/4"). Temperatures alternate between 30 ?C with light for 12 hours and 20 ?C in darkness for 12 hours per 24 hour cycle. Replication and sample size: either 4 reps of 50 seed each or 2 reps of 100 seed each for a total of 200 seeds. The first count is done 3 or 4 days after start of test. If needed, second and third counts are done weekly after first count.
TOP

ENO: 493850 Ammi & Orlaya
Duration
Temperature
Fluorescent Lights
7 days
10 ?C
---
12 hours
20 ?C
ON
12 hours
20 ?C
OFF
Seeds are placed on top of 3 blotter papers (1 blue, 2 white - in contact with seeds) moistened with tap water in plastic boxes (5" x 5-1/4") and kept at room temperature overnight; prechill at 10 ?C for 7 days. Transfer to germinator. Temperature at a constant 20 ?C with light for 12 hours and dark for 12 hours per 24 hour cycle. Replication and sample size: 4 reps of 50 seed each for a total of 200 seeds. The counts are done 7 and 21 days after start of test.
TOP

ENO: 493834 Anethum
Duration
Temperature
Fluorescent Lights
12 hours
30 ?C
ON
12 hours
20 ?C
OFF
Seeds are placed in folded paper towels moistened with tap water and placed in plastic tubs. These are kept at room temperature overnight and then transferred to a germinator. Temperatures alternate between 30 ?C with light for 12 hours and 20 ?C in darkness for 12 hours per 24 hour cycle. Replication and sample size: 4 reps of 50 seed each for a total of 200 seeds. Germination counts occur 7 and 21 days after start of test.
TOP

ENO: 493921 Angelica
Duration
Temperature
Fluorescent Lights
14 days
4 ?C
---
12 hours
25 ?C
ON
12 hours
15 ?C
OFF
Seeds are placed on top of 2 blue blotter papers moistened with 1 mM GA₃ in plastic boxes (5" x 5-1/4"); let sit overnight at room temperature. Prechill at 4 ?C for 14 days. Transfer to germinator with temperatures alternatingalternating between 25 ?C with light for 12 hours and 15 ?C in darkness for 12 hours per 24 hour cycle. Replication and sample size:4 reps of 50 seed each for a total of 200 seeds. Germination counts occur at 10, 17, and 28 days after start of the germination test.
TOP

ENO: 493835 Anthriscus
Duration
Temperature
Fluorescent Lights
12 hours
30 ?C
ON
12 hours
20 ?C
OFF
Seeds are placed on top of 3 blotter papers (1 blue, 2 white - in contact with seeds) moistened with tap water in plastic boxes (5" x 5-1/4") and kept at room temperature overnight. Transfer to germinator with temperatures alternating between 30 ?C with light for 12 hours and 20 ?C in darkness for 12 hours per 24 hour cycle. Replication and sample size: 4 reps of 50 seed each for a total of 200 seeds. The counts are done 7 and 21 days after start of test.
TOP

ENO: 493836 Bifora, Dalea, & Galega
Duration
Temperature
Fluorescent Lights
10 days
4 ?C
---
12 hours
25 ?C
ON
12 hours
15 ?C
OFF
Seeds are placed in folded paper towels moistened with tap water and placed in plastic tubs. These are kept at room temperature overnight; prechill at 4 ?C for 10 days. Transfer to germinator. Temperatures alternate between 25 ?C with light for 12 hours and 15 ?C in darkness for 12 hours per 24 hour cycle. Replication and sample size: 4 reps of 50 seed each for a total of 200 seeds. Germination counts occur 7, 14, and 21 days after start of test. Chip any remaining ungerminated seed at 21 days and then extend test 5 more days. Do final count and end test.
TOP

ENO: 152 Calamovilfa
Duration
Temperature
Fluorescent Lights
12 hours
30 ?C
ON
12 hours
20 ?C
OFF
Seeds are placed on top of 3 blotter papers (2 blue, 1 white - in contact with seeds) moistened with 0.1% KNO₃ solution in plastic boxes (5" x 5-1/4"). Temperatures alternate between 30 ?C with light for 12 hours and 20 ?C in darkness for 12 hours per 24 hour cycle. Replication and sample size: 4 reps of 50 seed each for a total of 200 seeds. Germination counts occur 4 and 14 days after start of test.
TOP

ENO: 491462 Celosia
Duration
Temperature
Fluorescent Lights
12 hours
30 ?C
ON
12 hours
20 ?C
OFF
Seeds are placed on top of 2 blue blotter papers moistened with tap water in plastic boxes (5" x 5-1/4"). Temperatures alternate between 30 ?C with light for 12 hours and 20 ?C in darkness for 12 hours per 24 hour cycle. Replication and sample size: 4 reps of 50 seed each for a total of 200 seeds. The first count is done 10 days after start of test. If needed, a second count is done 5 days after first count.
TOP

ENO: 491651 Chenopodium (cultivated)
Duration
Temperature
Fluorescent Lights
12 hours
30 ?C
ON
12 hours
20 ?C
OFF
Seeds are placed on top of 3 blotter papers (2 blue, 1 white - in contact with seeds) moistened with 0.1% KNO₃ solution in plastic boxes (5" x 5-1/4"). Temperatures alternate between 30 ?C with light for 12 hours and 20 ?C in darkness for 12 hours per 24 hour cycle. Replication and sample size: 4 reps of 50 seed each for a total of 200 seeds. The first count occurs 6 days after start of test.
TOP

ENO: 493487 Chenopodium (dormant/wild species)
Duration
Temperature
Fluorescent Lights
1 month
4 ?C
---
12 hours
30 ?C
ON
12 hours
20 ?C
OFF
Seeds are placed on top of 3 blotter papers (2 blue, 1 white - in contact with seeds) moistened with tap water in plastic boxes (5" x 5-1/4"). Leave at room temperature overnight; prechill at 4 ?C for one month. Transfer to germinator. Temperatures alternate between 30 ?C with light for 12 hours and 20 ?C in darkness for 12 hours per 24 hour cycle. Replication and sample size: 4 reps of 50 seed each for a total of 200 seeds. The first count occurs 6 days after transfer from cold treatment.

TOP
ENO: 493840 Coniocelinum & Oreoschimperella
Duration
Temperature
Fluorescent Lights
12 hours
30 ?C
ON
12 hours
20 ?C
OFF
Seeds are placed between blotter papers moistened with tap water in plastic boxes (5" x 5-1/4") with the following arrangement:
(top) 1 blue paper
seeds
1 white paper
(bottom) 1 blue paper
Let seeds imbibe overnight at room temperature then place in germinator. Temperatures alternate between 30 ?C with light for 12 hours and 20 ?C in darkness for 12 hours per 24 hour cycle. Replication and sample size: 4 reps of 50 seed each for a total of 200 seeds. Germination counts occur 7 and 21 days after start of test.
TOP

ENO: 492498 Coriander
Duration
Temperature
Fluorescent Lights
12 hours
15 ?C
ON
12 hours
15 ?C
OFF
Seeds are placed between blotter papers moistened with tap water in plastic boxes (5" x 5-1/4") with the following arrangement:
(top) 1 blue paper
seeds
1 white paper
(bottom) 1 blue paper
Temperature is constant at 15 ?C with light for 12 hours and darkness for 12 hours per 24 hour cycle. Replication and sample size: 4 reps of 50 seed each for a total of 200 seeds. The first count occurs 6 days after start of test.
TOP

ENO: 490362 Echinochloa (standard)
Duration
Temperature
Fluorescent Lights
12 hours
30 ?C
ON
12 hours
20 ?C
OFF
Seeds are placed on top of 2 blue blotter papers moistened with tap water in plastic boxes (5" x 5-1/4"). Temperatures alternate between 30 ?C with light for 12 hours and 20 ?C in darkness for 12 hours per 24 hour cycle. Replication and sample size: 4 reps of 50 seed each for a total of 200 seeds. Germination counts occur 4 and 10 days after start of test.
TOP

ENO: 494560 Echinochloa (side-by-side)
Duration
Temperature
Fluorescent Lights
12 hours
30 ?C
ON
12 hours
20 ?C
OFF
There are two sets to this test:
    1) Light set: alternating light and dark, and
    2) Dark set: constant dark.

Germinations are conducted in blotter boxes.
Scoring is done on 7, 14, and 21 days after the start of germination. IMPORTANT NOTE: First count is NOT to occur before 7 days.

If inventory on hand is > 6,000 seed, then:

    1) Light set:
           4 reps of 50 seed
           30C + light (12hrs) / 20 + dark (12hrs)
           Put seed on top of blotter papers.

    2) Dark set:
           4 reps of 50 seed
           Dark, 30C (12hrs) / 20C (12 hrs)
           Put seed in between dark, blue blotter papers
               (near center of papers),
           or cover boxes with aluminum foil.

If inventory on hand is 6,000 seed or less, then reduce sample size by conducting test on just 2 reps of 50 seed each..
TOP

ENO: 491625 Eryngium
Duration
Temperature
Fluorescent Lights
12 hours
30 ?C
ON
12 hours
20 ?C
OFF
Seeds are placed on top of 3 blotter papers (2 blue, 1 white - in contact with seeds) moistened with tap water in plastic boxes (5" x 5-1/4"). Leave at room temperature overnight. Transfer to germinator. Temperatures alternate between 30 ?C with light for 12 hours and 20 ?C in darkness for 12 hours per 24 hour cycle. Replication and sample size: 4 reps of 50 seed each for a total of 200 seeds. Germination counts occur 7 and 21 days after start of test.
TOP

ENO: 493851 Ferula & Levisticum
Duration
Temperature
Fluorescent Lights
7 days
10 ?C
---
12 hours
25 ?C
ON
12 hours
15 ?C
OFF
Seeds are placed on top of 3 blotter papers (1 blue, 2 white - in contact with seeds) moistened with tap water in plastic boxes (5" x 5-1/4") and kept at room temperature overnight; prechill at 10 ?C for 7 days. Transfer to germinator. Temperatures alternate between 25 ?C with light for 12 hours and 15 ?C in darkness for 12 hours per 24 hour cycle. Replication and sample size: 4 reps of 50 seed each for a total of 200 seeds. The counts are done 7 and 21 days after start of test.
TOP

ENO: 493814 Foeniculum
Duration
Temperature
Fluorescent Lights
12 hours
30 ?C
ON
12 hours
20 ?C
OFF
Seeds are placed between blotter papers moistened with tap water in plastic boxes (5" x 5-1/4") with the following arrangement:
(top) 1 blue paper
seeds
1 white paper
(bottom) 1 blue paper
Let seeds imbibe overnight at room temperature then place in germinator. Temperatures alternate between 30 ?C with light for 12 hours and 20 ?C in darkness for 12 hours per 24 hour cycle. Replication and sample size: 4 reps of 50 seed each for a total of 200 seeds. Germination counts occur 7 and 14 days after start of test.
TOP

ENO: 492586 Melilotus
Duration
Temperature
Fluorescent Lights
14 days
4 ?C
---
12 hours
20 ?C
ON
12 hours
20 ?C
OFF
Seeds are placed between blotter papers moistened with 0.1% KNO₃ in plastic boxes (5" x 5-1/4") with the following arrangement:
(top) 1 blue paper
seeds
1 white paper
(bottom) 1 blue paper
Let seeds imbibe overnight at room temperature; prechill at 4 ?C for 14 days. Transfer to germinator. Temperature is constant at 20 ?C with light for 12 hours and darkness for 12 hours per 24 hour cycle. Replication and sample size: 4 reps of 50 seed each for a total of 200 seeds. Germination counts occur 7 and 14 days after start of test.
TOP

ENO: 490363 Panicum & Misc. Grasses
Duration
Temperature
Fluorescent Lights
7 days
4 ?C
---
12 hours
30 ?C
ON
12 hours
15 ?C
OFF
Seeds are placed on top of 2 blue blotter papers moistened with with 0.1% KNO₃ solution in plastic boxes (5" x 5-1/4"). Leave at room temperature overnight; prechill at 4 ?C for 7 days. Transfer to germinator. Temperatures alternate between 30 ?C with light for 12 hours and 15 ?C in darkness for 12 hours per 24 hour cycle. Replication and sample size: 4 reps of 50 seed each for a total of 200 seeds. Germination counts occur 7 and 21 days after start of test.
TOP

ENO: 493899 Perilla
Duration
Temperature
Fluorescent Lights
12 hours
20 ?C
OFF
12 hours
20 ?C
OFF
Seeds are placed between 2 blotter papers moistened with tap water in plastic boxes (5" x 5-1/4") with the following arrangement:
(top) 1 white paper
seeds
(bottom) 1 white paper
Let seeds imbibe overnight at room temperature then place in germinator. Temperature is constant at 20 ?C with dark conditions. Replication and sample size: 4 reps of 50 seed each for a total of 200 seeds. Germination counts occur 5 and 14 days after start of test.
TOP

ENO: 493801 Petroselinum
Duration
Temperature
Fluorescent Lights
12 hours
30 ?C
ON
12 hours
20 ?C
OFF
Seeds are placed between blotter papers moistened with tap water in plastic boxes (5" x 5-1/4") with the following arrangement:
(top) 1 blue paper
seeds
1 white paper
(bottom) 1 blue paper
Let seeds imbibe overnight at room temperature then place in germinator. Temperatures alternate between 30 ?C with light for 12 hours and 20 ?C in darkness for 12 hours per 24 hour cycle. Replication and sample size: 4 reps of 50 seed each for a total of 200 seeds. Germination counts occur 7 and 14 days after start of test.
TOP

ENO: 490361 Setaria (cultivated)
Duration
Temperature
Fluorescent Lights
12 hours
30 ?C
ON
12 hours
20 ?C
OFF
Seeds are placed in folded paper towels moistened with tap water and placed in plastic tubs. Temperatures alternate between 30 ?C with light for 12 hours and 20 ?C in darkness for 12 hours per 24 hour cycle. Replication and sample size: 4 reps of 50 seed each for a total of 200 seeds. Germination counts occur 4 and 10 days after start of test.
TOP

ENO: 490385 Setaria (dormant/wild species)
Duration
Temperature
Fluorescent Lights
2 weeks
4 ?C
---
12 hours
30 ?C
ON
12 hours
15 ?C
OFF
Seeds are placed between blotter papers moistened with 0.1% KNO₃ in plastic boxes (5" x 5-1/4") with the following arrangement:
(top) 1 blue paper
seeds
1 white paper
(bottom) 1 blue paper
Let seeds imbibe overnight at room temperature then place in 4 ?C for 2 weeks. Transfer to germinator. Temperatures alternate between 30 ?C with light for 12 hours and 15 ?C in darkness for 12 hours per 24 hour cycle. Replication and sample size: 4 reps of 50 seed each for a total of 200 seeds. Germination counts occur 7 and 21 days after start of test.
TOP

ENO: 493852 Sium
Duration
Temperature
Fluorescent Lights
7 days
10 ?C
---
12 hours
25 ?C
ON
12 hours
15 ?C
OFF
Seeds are placed on top of 2 blue blotter papers moistened with tap water in plastic boxes (5" x 5-1/4"); make sure blotter paper is very wet. These are kept at room temperature overnight; prechill at 10 ?C for 7 days. Transfer to germinator. Temperatures alternate between 25 ?C with light for 12 hours and 15 ?C in darkness for 12 hours per 24 hour cycle. Replication and sample size: 4 reps of 50 seed each for a total of 200 seeds. The counts are done 7 and 21 days after start of test.
TOP

ENO: 491601 Spinacia
Duration
Temperature
Fluorescent Lights
Until seeds sprout
5 ?C
---
12 hours
25 ?C
ON
12 hours
15 ?C
OFF
Seeds are placed on top of 3 blotter papers (2 blue, 1 white - in contact with seeds) moistened with tap water in plastic boxes (5" x 5-1/4"). The plastic boxes are wrapped with parafilm to help maintain moisture during the long cold treatment. Temperature is a constant 5 ?C until seeds begin to sprout. Transfer to germinator. Temperatures alternate between 25 ?C with light for 12 hours and 15 ?C in darkness for 12 hours per 24 hour cycle. Replication and sample size: 4 reps of 50 seed each for a total of 200 seeds. Germination counts occur immediately and then 7, 14, 21, and 28 days after transfer from cold treatment.
TOP

ENO: 490972 Umbels
(includes Astrodaucus, Caucalis & Zizia)
Duration
Temperature
Fluorescent Lights
12 hours
25 ?C
ON
12 hours
15 ?C
OFF
Seeds are placed in folded paper towels moistened with tap water and placed in plastic tubs. Let sit overnight at room temperature. Transfer to germinator with temperatures alternating between 25 ?C with light for 12 hours and 15 ?C in darkness for 12 hours per 24 hour cycle. Replication and sample size: 4 reps of 50 seed each for a total of 200 seeds. Counts are done at 7, 14, and 21 days after start of test.

ENO: 491828 Amaranth & Cuminum
Duration	Temperature	Fluorescent Lights
12 hours	30 ?C	ON
12 hours	20 ?C	OFF
Seeds are placed on top of 2 blue blotter papers moistened with tap water in plastic boxes (5" x 5-1/4"). Temperatures alternate between 30 ?C with light for 12 hours and 20 ?C in darkness for 12 hours per 24 hour cycle. Replication and sample size: either 4 reps of 50 seed each or 2 reps of 100 seed each for a total of 200 seeds. The first count is done 3 or 4 days after start of test. If needed, second and third counts are done weekly after first count.

ENO: 493850 Ammi & Orlaya
Duration	Temperature	Fluorescent Lights
7 days	10 ?C	---
12 hours	20 ?C	ON
12 hours	20 ?C	OFF
Seeds are placed on top of 3 blotter papers (1 blue, 2 white - in contact with seeds) moistened with tap water in plastic boxes (5" x 5-1/4") and kept at room temperature overnight; prechill at 10 ?C for 7 days. Transfer to germinator. Temperature at a constant 20 ?C with light for 12 hours and dark for 12 hours per 24 hour cycle. Replication and sample size: 4 reps of 50 seed each for a total of 200 seeds. The counts are done 7 and 21 days after start of test.

ENO: 493834 Anethum
Duration	Temperature	Fluorescent Lights
12 hours	30 ?C	ON
12 hours	20 ?C	OFF
Seeds are placed in folded paper towels moistened with tap water and placed in plastic tubs. These are kept at room temperature overnight and then transferred to a germinator. Temperatures alternate between 30 ?C with light for 12 hours and 20 ?C in darkness for 12 hours per 24 hour cycle. Replication and sample size: 4 reps of 50 seed each for a total of 200 seeds. Germination counts occur 7 and 21 days after start of test.

ENO: 493921 Angelica
Duration	Temperature	Fluorescent Lights
14 days	4 ?C	---
12 hours	25 ?C	ON
12 hours	15 ?C	OFF
Seeds are placed on top of 2 blue blotter papers moistened with 1 mM GA₃ in plastic boxes (5" x 5-1/4"); let sit overnight at room temperature. Prechill at 4 ?C for 14 days. Transfer to germinator with temperatures alternatingalternating between 25 ?C with light for 12 hours and 15 ?C in darkness for 12 hours per 24 hour cycle. Replication and sample size:4 reps of 50 seed each for a total of 200 seeds. Germination counts occur at 10, 17, and 28 days after start of the germination test.

ENO: 493835 Anthriscus
Duration	Temperature	Fluorescent Lights
12 hours	30 ?C	ON
12 hours	20 ?C	OFF
Seeds are placed on top of 3 blotter papers (1 blue, 2 white - in contact with seeds) moistened with tap water in plastic boxes (5" x 5-1/4") and kept at room temperature overnight. Transfer to germinator with temperatures alternating between 30 ?C with light for 12 hours and 20 ?C in darkness for 12 hours per 24 hour cycle. Replication and sample size: 4 reps of 50 seed each for a total of 200 seeds. The counts are done 7 and 21 days after start of test.

ENO: 493836 Bifora, Dalea, & Galega
Duration	Temperature	Fluorescent Lights
10 days	4 ?C	---
12 hours	25 ?C	ON
12 hours	15 ?C	OFF
Seeds are placed in folded paper towels moistened with tap water and placed in plastic tubs. These are kept at room temperature overnight; prechill at 4 ?C for 10 days. Transfer to germinator. Temperatures alternate between 25 ?C with light for 12 hours and 15 ?C in darkness for 12 hours per 24 hour cycle. Replication and sample size: 4 reps of 50 seed each for a total of 200 seeds. Germination counts occur 7, 14, and 21 days after start of test. Chip any remaining ungerminated seed at 21 days and then extend test 5 more days. Do final count and end test.

ENO: 152 Calamovilfa
Duration	Temperature	Fluorescent Lights
12 hours	30 ?C	ON
12 hours	20 ?C	OFF
Seeds are placed on top of 3 blotter papers (2 blue, 1 white - in contact with seeds) moistened with 0.1% KNO₃ solution in plastic boxes (5" x 5-1/4"). Temperatures alternate between 30 ?C with light for 12 hours and 20 ?C in darkness for 12 hours per 24 hour cycle. Replication and sample size: 4 reps of 50 seed each for a total of 200 seeds. Germination counts occur 4 and 14 days after start of test.

ENO: 491462 Celosia
Duration	Temperature	Fluorescent Lights
12 hours	30 ?C	ON
12 hours	20 ?C	OFF
Seeds are placed on top of 2 blue blotter papers moistened with tap water in plastic boxes (5" x 5-1/4"). Temperatures alternate between 30 ?C with light for 12 hours and 20 ?C in darkness for 12 hours per 24 hour cycle. Replication and sample size: 4 reps of 50 seed each for a total of 200 seeds. The first count is done 10 days after start of test. If needed, a second count is done 5 days after first count.

ENO: 491651 Chenopodium (cultivated)
Duration	Temperature	Fluorescent Lights
12 hours	30 ?C	ON
12 hours	20 ?C	OFF
Seeds are placed on top of 3 blotter papers (2 blue, 1 white - in contact with seeds) moistened with 0.1% KNO₃ solution in plastic boxes (5" x 5-1/4"). Temperatures alternate between 30 ?C with light for 12 hours and 20 ?C in darkness for 12 hours per 24 hour cycle. Replication and sample size: 4 reps of 50 seed each for a total of 200 seeds. The first count occurs 6 days after start of test.

ENO: 493487 Chenopodium (dormant/wild species)
Duration	Temperature	Fluorescent Lights
1 month	4 ?C	---
12 hours	30 ?C	ON
12 hours	20 ?C	OFF
Seeds are placed on top of 3 blotter papers (2 blue, 1 white - in contact with seeds) moistened with tap water in plastic boxes (5" x 5-1/4"). Leave at room temperature overnight; prechill at 4 ?C for one month. Transfer to germinator. Temperatures alternate between 30 ?C with light for 12 hours and 20 ?C in darkness for 12 hours per 24 hour cycle. Replication and sample size: 4 reps of 50 seed each for a total of 200 seeds. The first count occurs 6 days after transfer from cold treatment.

ENO: 493840 Coniocelinum & Oreoschimperella
Duration	Temperature	Fluorescent Lights
12 hours	30 ?C	ON
12 hours	20 ?C	OFF
Seeds are placed between blotter papers moistened with tap water in plastic boxes (5" x 5-1/4") with the following arrangement: (top) 1 blue paper seeds 1 white paper (bottom) 1 blue paper Let seeds imbibe overnight at room temperature then place in germinator. Temperatures alternate between 30 ?C with light for 12 hours and 20 ?C in darkness for 12 hours per 24 hour cycle. Replication and sample size: 4 reps of 50 seed each for a total of 200 seeds. Germination counts occur 7 and 21 days after start of test.

ENO: 492498 Coriander
Duration	Temperature	Fluorescent Lights
12 hours	15 ?C	ON
12 hours	15 ?C	OFF
Seeds are placed between blotter papers moistened with tap water in plastic boxes (5" x 5-1/4") with the following arrangement: (top) 1 blue paper seeds 1 white paper (bottom) 1 blue paper Temperature is constant at 15 ?C with light for 12 hours and darkness for 12 hours per 24 hour cycle. Replication and sample size: 4 reps of 50 seed each for a total of 200 seeds. The first count occurs 6 days after start of test.

ENO: 490362 Echinochloa (standard)
Duration	Temperature	Fluorescent Lights
12 hours	30 ?C	ON
12 hours	20 ?C	OFF
Seeds are placed on top of 2 blue blotter papers moistened with tap water in plastic boxes (5" x 5-1/4"). Temperatures alternate between 30 ?C with light for 12 hours and 20 ?C in darkness for 12 hours per 24 hour cycle. Replication and sample size: 4 reps of 50 seed each for a total of 200 seeds. Germination counts occur 4 and 10 days after start of test.

ENO: 494560 Echinochloa (side-by-side)
Duration	Temperature	Fluorescent Lights
12 hours	30 ?C	ON
12 hours	20 ?C	OFF
There are two sets to this test: 1) Light set: alternating light and dark, and 2) Dark set: constant dark. Germinations are conducted in blotter boxes. Scoring is done on 7, 14, and 21 days after the start of germination. IMPORTANT NOTE: First count is NOT to occur before 7 days. If inventory on hand is > 6,000 seed, then: 1) Light set: 4 reps of 50 seed 30C + light (12hrs) / 20 + dark (12hrs) Put seed on top of blotter papers. 2) Dark set: 4 reps of 50 seed Dark, 30C (12hrs) / 20C (12 hrs) Put seed in between dark, blue blotter papers (near center of papers), or cover boxes with aluminum foil. If inventory on hand is 6,000 seed or less, then reduce sample size by conducting test on just 2 reps of 50 seed each..

ENO: 491625 Eryngium
Duration	Temperature	Fluorescent Lights
12 hours	30 ?C	ON
12 hours	20 ?C	OFF
Seeds are placed on top of 3 blotter papers (2 blue, 1 white - in contact with seeds) moistened with tap water in plastic boxes (5" x 5-1/4"). Leave at room temperature overnight. Transfer to germinator. Temperatures alternate between 30 ?C with light for 12 hours and 20 ?C in darkness for 12 hours per 24 hour cycle. Replication and sample size: 4 reps of 50 seed each for a total of 200 seeds. Germination counts occur 7 and 21 days after start of test.

ENO: 493851 Ferula & Levisticum
Duration	Temperature	Fluorescent Lights
7 days	10 ?C	---
12 hours	25 ?C	ON
12 hours	15 ?C	OFF
Seeds are placed on top of 3 blotter papers (1 blue, 2 white - in contact with seeds) moistened with tap water in plastic boxes (5" x 5-1/4") and kept at room temperature overnight; prechill at 10 ?C for 7 days. Transfer to germinator. Temperatures alternate between 25 ?C with light for 12 hours and 15 ?C in darkness for 12 hours per 24 hour cycle. Replication and sample size: 4 reps of 50 seed each for a total of 200 seeds. The counts are done 7 and 21 days after start of test.

ENO: 493814 Foeniculum
Duration	Temperature	Fluorescent Lights
12 hours	30 ?C	ON
12 hours	20 ?C	OFF
Seeds are placed between blotter papers moistened with tap water in plastic boxes (5" x 5-1/4") with the following arrangement: (top) 1 blue paper seeds 1 white paper (bottom) 1 blue paper Let seeds imbibe overnight at room temperature then place in germinator. Temperatures alternate between 30 ?C with light for 12 hours and 20 ?C in darkness for 12 hours per 24 hour cycle. Replication and sample size: 4 reps of 50 seed each for a total of 200 seeds. Germination counts occur 7 and 14 days after start of test.

ENO: 492586 Melilotus
Duration	Temperature	Fluorescent Lights
14 days	4 ?C	---
12 hours	20 ?C	ON
12 hours	20 ?C	OFF
Seeds are placed between blotter papers moistened with 0.1% KNO₃ in plastic boxes (5" x 5-1/4") with the following arrangement: (top) 1 blue paper seeds 1 white paper (bottom) 1 blue paper Let seeds imbibe overnight at room temperature; prechill at 4 ?C for 14 days. Transfer to germinator. Temperature is constant at 20 ?C with light for 12 hours and darkness for 12 hours per 24 hour cycle. Replication and sample size: 4 reps of 50 seed each for a total of 200 seeds. Germination counts occur 7 and 14 days after start of test.

ENO: 490363 Panicum & Misc. Grasses
Duration	Temperature	Fluorescent Lights
7 days	4 ?C	---
12 hours	30 ?C	ON
12 hours	15 ?C	OFF
Seeds are placed on top of 2 blue blotter papers moistened with with 0.1% KNO₃ solution in plastic boxes (5" x 5-1/4"). Leave at room temperature overnight; prechill at 4 ?C for 7 days. Transfer to germinator. Temperatures alternate between 30 ?C with light for 12 hours and 15 ?C in darkness for 12 hours per 24 hour cycle. Replication and sample size: 4 reps of 50 seed each for a total of 200 seeds. Germination counts occur 7 and 21 days after start of test.

ENO: 493899 Perilla
Duration	Temperature	Fluorescent Lights
12 hours	20 ?C	OFF
12 hours	20 ?C	OFF
Seeds are placed between 2 blotter papers moistened with tap water in plastic boxes (5" x 5-1/4") with the following arrangement: (top) 1 white paper seeds (bottom) 1 white paper Let seeds imbibe overnight at room temperature then place in germinator. Temperature is constant at 20 ?C with dark conditions. Replication and sample size: 4 reps of 50 seed each for a total of 200 seeds. Germination counts occur 5 and 14 days after start of test.

ENO: 493801 Petroselinum
Duration	Temperature	Fluorescent Lights
12 hours	30 ?C	ON
12 hours	20 ?C	OFF
Seeds are placed between blotter papers moistened with tap water in plastic boxes (5" x 5-1/4") with the following arrangement: (top) 1 blue paper seeds 1 white paper (bottom) 1 blue paper Let seeds imbibe overnight at room temperature then place in germinator. Temperatures alternate between 30 ?C with light for 12 hours and 20 ?C in darkness for 12 hours per 24 hour cycle. Replication and sample size: 4 reps of 50 seed each for a total of 200 seeds. Germination counts occur 7 and 14 days after start of test.

ENO: 490361 Setaria (cultivated)
Duration	Temperature	Fluorescent Lights
12 hours	30 ?C	ON
12 hours	20 ?C	OFF
Seeds are placed in folded paper towels moistened with tap water and placed in plastic tubs. Temperatures alternate between 30 ?C with light for 12 hours and 20 ?C in darkness for 12 hours per 24 hour cycle. Replication and sample size: 4 reps of 50 seed each for a total of 200 seeds. Germination counts occur 4 and 10 days after start of test.

ENO: 490385 Setaria (dormant/wild species)
Duration	Temperature	Fluorescent Lights
2 weeks	4 ?C	---
12 hours	30 ?C	ON
12 hours	15 ?C	OFF
Seeds are placed between blotter papers moistened with 0.1% KNO₃ in plastic boxes (5" x 5-1/4") with the following arrangement: (top) 1 blue paper seeds 1 white paper (bottom) 1 blue paper Let seeds imbibe overnight at room temperature then place in 4 ?C for 2 weeks. Transfer to germinator. Temperatures alternate between 30 ?C with light for 12 hours and 15 ?C in darkness for 12 hours per 24 hour cycle. Replication and sample size: 4 reps of 50 seed each for a total of 200 seeds. Germination counts occur 7 and 21 days after start of test.

ENO: 493852 Sium
Duration	Temperature	Fluorescent Lights
7 days	10 ?C	---
12 hours	25 ?C	ON
12 hours	15 ?C	OFF
Seeds are placed on top of 2 blue blotter papers moistened with tap water in plastic boxes (5" x 5-1/4"); make sure blotter paper is very wet. These are kept at room temperature overnight; prechill at 10 ?C for 7 days. Transfer to germinator. Temperatures alternate between 25 ?C with light for 12 hours and 15 ?C in darkness for 12 hours per 24 hour cycle. Replication and sample size: 4 reps of 50 seed each for a total of 200 seeds. The counts are done 7 and 21 days after start of test.

ENO: 491601 Spinacia
Duration	Temperature	Fluorescent Lights
Until seeds sprout	5 ?C	---
12 hours	25 ?C	ON
12 hours	15 ?C	OFF
Seeds are placed on top of 3 blotter papers (2 blue, 1 white - in contact with seeds) moistened with tap water in plastic boxes (5" x 5-1/4"). The plastic boxes are wrapped with parafilm to help maintain moisture during the long cold treatment. Temperature is a constant 5 ?C until seeds begin to sprout. Transfer to germinator. Temperatures alternate between 25 ?C with light for 12 hours and 15 ?C in darkness for 12 hours per 24 hour cycle. Replication and sample size: 4 reps of 50 seed each for a total of 200 seeds. Germination counts occur immediately and then 7, 14, 21, and 28 days after transfer from cold treatment.

ENO: 490972 Umbels (includes Astrodaucus, Caucalis & Zizia)
Duration	Temperature	Fluorescent Lights
12 hours	25 ?C	ON
12 hours	15 ?C	OFF
Seeds are placed in folded paper towels moistened with tap water and placed in plastic tubs. Let sit overnight at room temperature. Transfer to germinator with temperatures alternating between 25 ?C with light for 12 hours and 15 ?C in darkness for 12 hours per 24 hour cycle. Replication and sample size: 4 reps of 50 seed each for a total of 200 seeds. Counts are done at 7, 14, and 21 days after start of test.

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U.S. DEPARTMENT OF AGRICULTURE

Plant Introduction Research: Ames, IA

Amaranth	491828	Ferula	493851
Ammi	493850	Foeniculum	493814
Anethum	493834	Galega	493836
Angelica	493921	Grasses	490363
Anthriscus	493835	Levisticum	493851
Astrodaucus	490972	Melilotus	492586
Bifora	493836	Oreoschimperella	493840
Calamovilfa	152	Orlaya	493850
Caucalis	490972	Panicum	490363
Celosia	491462	Perilla	493899
Chenopodium (cultivated)	491651	Petroselinum	493801
Chenopium (wild)	493487	Setaria (cultivated)	490361
Coniocelinum	493840	Setaria (wild)	490385
Coriander	492498	Sium	493852
Cuminum	491828	Spinacia	491601
Dalea	493836	Umbels	490972
Echinochloa (standard)	490362	Zizia	490972
Echinochloa (side-by-side)	494560
Eryngium	491625