Vectors & Control Elements

1 - Project Overview 2 - Vectors & Control Elements 3 - Laboratory Protocols 4 - Photo Gallery

We make these plasmid maps of the vectors and control elements used in our lab available for viewing and downloading. To view them, you must download and install Adobe Reader• from Adobe Systems, Inc. Please contact Dr. Bill Belknap for sequences and maps.

Control Elements

Granule Bound Starch Synthase (GBSS) promoter
View Map(PDF)

• HindII-EcoRI fragment in pCR vector
• High-level general tuber and root expression
• Ref: Van der Lief, et. al., Mol. Gen. Genet., 228:240-248, 1991.

Ubiquitin 3 Promoter
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• GUS expression cassette in pUC 19 vector
• Constitutive expression
• Ref: Garbarino et. al., Plant Physiology 109: 1371-1378, 1995.

Ubiquitin 3 poly ubiquitin fusion promoter
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• GUS expression cassette in pUC 19 vector
• High-level constitutive protein expression
• Ref: Garbarino et. al., Plant Physiology 109: 1371-1378, 1995.

Ubiquitin 3 Terminator
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• EcoRI insert in pCR vector
• All-purpose polyadenlyation signal

Patatin Class I Promoter in pUC 19 vector
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• Tuber specific expression
• Ref: Blundy, et. al., Plant Molecular Biology, 16:153-160, 1991.

Granule Bound Starch Synthase (GBSS) promoter + Ubiquitin3 terminator
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• HindIII-EcoRI expression cassette with BamHI cloning site for gene insertion
• Expression in tubers and roots

409s-MCS-409T Detail
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409s-MCS-409T in pBinplus/ARS
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Binary Vectors

pBINPLUS-ARS
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• Binary vector developed from pBINPLUS
• Complete sequence available in GenBank accession DQ320121

ubi3-GUS-ubi3T
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• in pBINPLUS/ARS

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