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United States Department of Agriculture

Agricultural Research Service

John Hartung--Selected Publications
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Peer Reviewed Publications

Wenbin Li, Diva C. Teixeira, John S. Hartung, Qi Huang, Yongping Duan, Lijuan Zhou, Jianchi Chen, Hong Lin, Silvio Lopes, A. Juliano Ayres, and Laurene Levy. 2013. Development and Systematic Validation of Qpcr Assays for Rapid and Reliable Differentiation of Xylella Fastidiosa Strains Causing Citrus Variegated Chlorosis. Journal of Microbiological Methods 92: 79-89.

Ananthakrishnan, G., Choudhary, N. Roy, A., Postnikova, E., Hartung, J.S., Stone, A.L., Damsteegt, V.D., Schneider, W.L., and Brlansky, R.H. Development of primers and probes for genus and species specific detection of 'Candidatus Liberibacter species' by real-time PCR. Accepted.

Kuykendall, L.D., Shao, J., Hartung, J.S. 'Ca. Liberibacter Asiaticus' Proteins Orthologous with pSymA-Encoded Proteins of Sinorhizobium meliloti: Hypothetical Roles in Plant Host Interaction.PLoS ONE 7(6): e38725.

Kuykendall, L.D., Shao, J., Hartung, J.S. Conservation of gene order and content in 'Candidatus Liberibacter asiaticus' and Other Rhizobiales. PLoS ONE 7(4): e34673.

Kuykendall, L.D., Shao, J., Hartung, J.S. Conservation of gene order and content in 'Candidatus Liberibacter asiaticus' and Other Rhizobiales. PLoS ONE 7(4): e34673.

Hartung, J.S., Shao, J. Kuykendall, L.D. 2011. Comparison of the 'Ca. Liberibacter asiaticus' genome adapted for an intracellular lifestyle with other members of the Rhizobiales. PLoS ONE 6(8):e23289.

Chen, J., Deng, X., Civerolo, E.L., Lee, R.F., Jones, J.B., Zhou, C., Hartung, J.S., Manjunath, K.L., Brlansky, R.H. 2011. "Candidatus Liberibacter species": without Koch's postulates completed, can the bacterium be considered as the causal agent of citrus huanglongbing (yellow shoot disease)? Acta Phytopathologica Sinica 41(2): 113-117.

Nunney Leonard, Xiaoli Yuan, Robin Bromley, John Hartung, Mauricio Montero-Astúa, Lisela Moreira, Beatriz Ortiz and Richard Stouthamer. 2010. Population Genomic Analysis of a Bacterial Plant Pathogen: Novel Insight into the Origin of Pierce's Disease of Grapevine in the U.S. PLoS One 5(11):e15488.

Hartung, John S., Halbert, Susan E., Pelz-Stelinski, Kirsten, Brlansky, Ronald H., Chen, Chunxian and Gmitter, Fred. 2010. Lack of evidence for transmission of 'Ca. Liberibacter asiaticus through seed collected from affected fruit. Plant Disease 94(10):1200-1205.

Roy, A., Ananthakrishnan, G., Hartung, J.S. and Brlansky, R.H. 2010. Development and application of a multiplex reverse transcription polymerase chain reaction assay for screening a global collection of Citrus tristeza virus isolates. Phytopathology 100: 1077-1088.

Hartung, John S., Paul, Cristina, Achor, Diann and Brlansky, R.H. 2010. Colonization of dodder, Cuscuta indecora, by 'Candidatus Liberibacter asiaticus' and 'Ca. L. americanus'. Phytopathology 100:756-762.

Li, W., Levy, L., and Hartung, J.S. 2009. Quantitative Distribution of Candidatus Liberibacter asiaticus in Citrus Plants and Fruits Infected by Citrus Huanglongbing. Phytopathology 99:139-144.

Montero-Astua, M., Chacon-Diaz, C., Aguilar, E., Rodriguez, C.M., Villalobos, W., Rivera, C., Hartung, J.S. 2008. Isolation and molecular characterization of Xylella fastidiosa from coffee plants in Costa Rica. The J. of Microbiology 46(5):482-490.

Li, R., Mock, R.G., Huang, Q., Abad, J., Hartung, J.S., Kinard, G.R. 2008. A reliable and inexpensive method ofnucleic acid extraction for the PCR-based detection of diverse plant pathogens. J. Virological Methods doi:10.1016/j.jviromet.2008.09.008

Turechek, W., Hartung, J. and McCallister, J. 2008. Development and Optimization of a Real Time Detection Assay for Xanthomonas fragariae in Strawberry Crown Tissue with Receiver Operating Characteristic (ROC) Curve Analysis. Phytopathology 98(3):359-368.

Li, W., Li, D., Twieg, E., Hartung, J.S. and Levy, L. 2008. Optimized quantification of unculturable Candidatus Liberibacter species causing citrus Huanglongbing in host plants by real time PCR. Plant Disease 92(6): 854-861.

M. Montero-Astúa, G. Saborío-R., C. Chacón-Díaz, W. Villalobos, L. Moreira, J. S. Hartung, and C. Rivera. 2008. First Report of Xylella fastidiosa in avocado. Plant Disease 92(1): 175.

Lacava, P.T., Araújo, W.L., Azevedo, J.L., Li, W. and Hartung, J.S. 2007. The Endophyte Curtobacterium flaccumfaciens Reduces Symptoms Caused by Xylella fastidiosa in Periwinkle, Catharanthus roseus (L.). J. Microbiol 45(5): 388-393. Li, W., Song, Q., Brlansky, R. and Hartung, J.S. 2007. Genetic diversity of citrus canker pathogens preserved in herbarium specimens. PNAS USA 104 (47):18427-18432.

Montero-Astua, M.,Hartung, J.S.,Aguilar, E.,Chacon, C., Li, W.,Albertazzi, F.J., and Rivera, C. 2007. Genetic diversity of Xylella fastidiosa strains from Costa Rica, Sao Paulo, Brazil and the United States of America. Phytopathology 97 (10):1338-1347.

Li, W., Hartung, J.S. and Levy, L. 2007. Evaluation of DNA amplification methods for improved detection of "Candidatus Liberibacter species" associated with citrus Huanglongbing. Plant Disease 91:51-58.

Li, W. Hartung, J.S. and Levy, L.A. 2006. Quantitative real-time PCR for detection and identification of Candidatus Liberibacter species associated with Citrus huanglongbing. J. Microbiological Methods 65(2):237-246. DOI 10.1016/j.mimet.2005.07.014

Lacava, P.T., Li, W., Araujo, W., Azevedo, J.L. and Hartung, J.S. 2006.
Rapid, specific and quantitative assays for the detection of the endophytic bacterium Methylobacterium mesophilicum in plants. J. Microbiological Methods 65(3):535-541. DOI 10.1016/j.mimet.2005.09.015

Colletta, Filho, H.D., Takita, M.A., de Souza, A.A., Neto, J.R., Destefano, S.A.L., Hartung, J.S. and Machado, M.A. 2005. Primers based on the rpf gene region provide improved detection of Xanthomonas axonopodis pv citri in naturally and artificially infected citrus plants. J. Applied Microbiology 100(2):279-285.

Li, W., Brlansky, R.H. and Hartung, J.S. 2006. Amplification of DNA of Xanthomonas axonopodis pv. citri from historic citrus canker specimens. J. Microbiological Methods 65:237-246. DOI Information: 10.1016/j.mimet.2005.07.014

Zhang, A. and Hartung, J.S. 2005.
Phenylacetaldehyde o-methyloxime: a volatile compound produced by grapefruit leaves infected with the citrus canker pathogen, Xanthomonas axonopodis pv. citri. J. Food and Agricultural Chemistry 53 (13): 5134-5137.

Belasque Jr., J., Parra-Pedrazolli, A.L., Rodrigues Neto, J., Yamamoto, P.T., Chagas, M.C.M., Parra, J.R.P., Vinyard, B.T., Hartung, J.S. 2005. Adult Citrus Leafminers (Phyllocnistis citrella) are not Efficient Vectors for Xanthomonas axonopodis pv. citri. Plant Disease 89(6):590-594.

Hartung, J.S. 2004. A proven model for working with exotic plant pathogens with biological security and low cost. Phytopathology News 38(12):162-163.

Huang, Q., Brlansky, R.H., Barnes, L., Li, W.-B. and Hartung, J.S. 2004. First report of oleander leaf scorch caused by Xylella fastidiosa in Texas. Plant Disease 88(9): 1049.

Qin, X. and Hartung, J.S. 2004. Expression of Green fluorescent Protein in Xylella fastidiosa is Affected by Passage Through Plants. Current Microbiology 49 (3): 211-216.

Qi Huang, W.-B. Li and John S. Hartung. 2003. Association of Xylella fastidiosa with leaf scorch in Japanese beech bonsai." Canadian Journal of Plant Pathology 25: 401-405.

W.-B Li, W. D. Pria, Jr., P. M. Lacava, J. S. Hartung. 2003. Presence of Xylella fastidiosa in Sweet Orange Fruit and Seeds and its Transmission to Seedlings. Phytopathology 93 (8):953-958.

Lewers, K.S.,Maas, J.L.,Hokanson, S.C.,Gouin, C.,Hartung, J.S. 2003. Inheritance of resistance in strawberry to bacterial angular leafspot disease caused by Xanthomonas fragariae. Journal of the American Society for Horticultural Science 128 (2):209-212.

Hartung, J.S. Phytosanitary Quarantine as a Disease Control Measure. In Encyclopedia of Pest Management. Edited by David Pimental. Marcel-Dekker, Inc. New York. 2003. DOI: 10.1081/E-EPM 120009964.

Brlansky, R.H., Damsteegt, V.D. and Hartung, J.S. 2002. Transmission of the citrus variegated chlorosis bacterium, Xylella fastidiosa, with the Florida sharpshooter, Oncometopia nigricans Walker. Plant Disease 86: 1237-1239.

Chen, J., Hartung, J.S., Chang, C.J., Vidaver, A.K. 2002. An evolutionary perspective of Pierce's disease of grapevine, citrus variegated chlorosis, and mulberry leaf scorch diseases. Current Microbiology 45(6)423-428.

Pria, Jr., W.D., Lacava, P.M., Li, W., Miranda, V.S., Costa, P.I., Farias, P.R.S., Hartung, J.S., Pereira and E.O., Francischini, F.J.B. 2002. Efeito da Xylella fastidosa em frutos and sementes de laranja doce (Citrus sinensis [L.] Osbeck) afetados pela CVC. Laranja, Cordeiropolis 23(1):183-202.

Li, W., Zhou, C., Pria, Jr., W.D., Teixera, D.C., Miranda, V.S., Pereira, A.J., He, C.-X., Costa, P.I. and Hartung, J.S. 2002. Citrus and coffee strains of Xylella fastidiosa induce Pierce's disease of grapevine. Plant Disease 86:1206-1210

Maas, J.L., Gouin, C., Hokanson, S.C. and Hartung, J.S. 2002. Strawberry parent clones US 4808 and US 4809 resistant to Bacterial Angular Leafspot Disease caused by Xanthomonas fragariae. HortScience: 37(4):716-717.

Chagas, Marcone C.M., Parra, Jose, R.P., Namekata, T., Hartung, J.S., Yamamoto, P.T. 2001. Phyllocnistis citrella Stainton (Lepidoptera: Gracillariidae) and its relationship with the citrus canker bacterium Xanthomonas axonopodis pv citri in Brazil. Neotropical Entomology 30(1):55-59.

Huang, Q. and Hartung, J.S. 2001. Cloning and Sequence Analysis of a Citrus Yellow Mosaic Virus Genome Infectious to Sweet Orange via Agrobacterium-Mediated Inoculation. J. General Virology 82(10): 2549-2558.

Qin, X. and Hartung, J.S. 2001. Construction of a shuttle vector and transformation of Xylella fastidiosa with plasmid DNA. Current Microbiology 43:158-162

Li, W.B., Pria Jr., W.D., Teixera, D.C., Miranda, V.S., Ayres, A.J., Franco, C.F., Costa, M.G., He, C.X., Costa, P.I. and Hartung, J.S. 2001. Coffee Leaf Scorch caused by a strain of Xylella fastidiosa from citrus. Plant Disease 85 (5):501-505.

Qin, X., Miranda, V.S. , Machado, M., Lemos, E. and Hartung, J.S. 2001. An evaluation of the Genetic Diversity of Xylella fastidiosa isolated from Diseased Citrus and Coffee. Phytopathology 91(6):599-605.

Chen, J., Jarret, R.L., Qin, X., Hartung, J.S., Chang, C.J. and Hopkins, D.L. 2000. 16SrDNA Analysis of Xylella fastidiosa. Systematic and Applied Microbiology 23:349-354.

He, C.X., Li, W.B., Ayres, A.J., Hartung, J.S., Miranda, V.S. and Teixeira, D.C. 2000. Distribution of Xylella fastidiosa in citrus rootstocks and transmission of citrus variegated chlorosis between sweet orange (C. sinensis (L.) Osbeck) plants through natural root grafts. Plant Disease 84(6): 622-626.

Da Costa, P.I., Franco, C.F., Miranda, V.S., Teixeira, D.C. and Hartung, J.S. 2000. Strains of Xylella fastidiosa rapidly distinguished by Arbitrarily Primed-PCR. Current Microbiology 40:279-282.

Maas, J.L., Gouin-Behe, C., Hartung, J.S. and Hokanson, S.C. 2000. Sources of resistance for two differentially pathogenic strains of Xanthomonas fragariae in Fragaria genotypes. HortScience 35(1): 128-131.

Hartung, J.S. 1999. Quarantine testing of prohibited germplasm by the USDA at Beltsville, MD. Fruit Varieties Journal 53(1):53-58.

Verniére, C., Hartung, J.S., Pruvost, O.P., Civerolo, E.L., Alvarez, A.M., Maestri, P. and Luisetti, J. 1998. Characterization of phenotypically distinct strains of Xanthomonas axonopodis pv. citri from Southwest Asia. European Journal of Plant Pathology 104: 477-487.

de Lima, J. E. O. , Miranda, V.S., Hartung, J.S., Brlansky, R.H., Coutinho, A., Roberto, S.R. and Carlos, E.F. 1998. Coffee Leaf Scorch Bacterium: Axenic Culture, Pathogenicity, and Comparison with Xylella fastidiosa of Citrus. Plant Disease 82(1): 94-97.

Pruvost, O., Vernière, C., Hartung, J., Gottwald, T. and Quetelard, H. 1997. Towards an improvement of citrus canker control in Reunion island. Fruits 52(6):375-382.

Pooler, M.R., J.S. Hartung and R.G. Fenton. 1997. Sequence analysis of a 1296-nucleotide plasmid from Xylella fastidiosa. FEMS Microbiology Letters 155: 217-222.

Pooler, M.R., Myung, I.S., Bentz, J., Sherald, J. and Hartung, J.S. 1997. Detection of Xylella fastidiosa in potential insect vectors by immunomagnetic separation and nested polymerase chain reaction. Letters in Applied Microbiology 25:123-126.

Pooler, M. R., Ritchie, D. F. and Hartung, J. S. 1996. Genetic Relationships among Strains of Xanthomonas fragariae based on Random Amplified Polymorphic DNA PCR, Repetitive Extragenic Palindromic PCR, and Enterobacterial Repetitive Intergenic Consensus PCR Data and Generation of Multiplexed PCR Primers Useful for the Identification of this Phytopathogen. Appl. Environ. Microbiol. 62(9):3121-3127.

Hartung, J.S., Pruvost, O.P., Villemot, I. and Alvarez, A. 1996. Rapid and Sensitive Colorimetric Detection of Xanthomonas campestris pv. citri by a Nested Polymerase Chain Reaction Assay. Phytopathology 86:95-101.

Roberts, D.P., Marty, A.M., Dery, P.D. and Hartung, J.S. 1996. Isolation and modulation of growth of a colonization-impaired strain of Enterobacter cloacae in cucumber spermosphere. Can. J. Microbiol. 42:196-201.

Roberts, D.P., Dery, P.D. and Hartung, J.S. 1996. Peptide utilization and colonization of corn, radish, and wheat spermosphere by Enterobacter cloacae. Soil Biol. Biochem. :28(8):1109-1101.

Roberts, D.P., Marty, A.M., Dery, P.D. Yucel, I. and Hartung, J.S. 1996. >Amino Acids as reduced carbon sources for Enterobacter cloacae during colonization of the spermospheres of crop plants. Soil Biol. Biochem. :28(8):1015-1020.

Pooler, M. R. and Hartung, J. S. 1995. Specific PCR detection and identification of Xylella fastidiosa strains that cause citrus variegated chlorosis. Current Microbiology 31(6):377-381.

Hartung, J.S. and Pruvost, O.P. 1995. Detection of Xanthomonas campestris pv. citri by hybridization and polymerase chain reaction assays. United States Patent # 5,420,009. May 30, 1995.

Pooler, M. R. and Hartung, J. S. 1995. Genetic Relationships Among Strains of Xylella fastidiosa Based on RAPD-PCR Data. Current Microbiology 31: 134-137.

Hartung, John S., Beretta, J., Brlansky, R.H., Spisso, J. and Lee, R.F. 1994. Citrus Variegated Chlorosis: Axenic Culture, Pathogenicity, and Serological Relationships with other Strains of Xylella fastidiosa. Phytopathology 84: 591-597.

Lee, R.F., Beretta, M.J.G., Hartung, J.S., Hooker, M.E. and Derrick, K.S. 1993. Xylella fastidiosa: Agente Causal da Clorose Variegada dos Citros. Laranja, Cordeiropolis 14(1): 157-166.

Lee, Richard F., Beretta, Maria Julia G., Hartung, John S., Hooker, M.E. And K.E. Derrick. 1993. Citrus Variegated Chlorosis: confirmation of a Xylella fastidiosa as the causal agent. Summa Phytopathologica 19:123-125.

Hartung, John S., Daniel, J.F. and Pruvost, O.P. 1993. Detection of Xanthomonas campestris pv. Citri by the Polymerase Chain Reaction Method. Appl. Environ. Microbiol. 59(4):1143-1148.

Roberts, D. P. Sheets, C. J. and J. S. Hartung. 1992. Evidence for proliferation of Enterobacter cloacae on carbohydrates in cucumber and pea spermosphere. Can J. Microbiol. 38:1128-1134.

Wells, J., E. L. Civerolo and J. S. Hartung, 1993. Cellular fatty acid composition of nine pathovars of X. campestris. J. Phytopathology 138:125-136.

Hartung, J. S. 1992. Plasmid-based hybridization probes for detection and identification of Xanthomonas campestris pv. citri. Plant Disease 76: 889-893.

Pruvost, O. P., J. S. Hartung, E. L. Civerolo, C. DuBois and X. Perrier. 1992. Plasmid DNA fingerprints distinguish pathotypes of Xanthomonas campestris pv. citri, the causal agent of citrus bacterial canker disease. Phytopathology 82: 485-490.

Gottwald, T. R., A. M. Alvarez, J. S. Hartung and A. A. Benedict. 1991. Diversity of Xanthomonas campestris pv. citrumelo strains associated with epidemics of citrus bacterial leaf spot in Florida citrus nurseries: Correlation of detached leaf, monoclonal antibody, and restriction fragment length polymorphism assays. Phytopathology 81: 749-753.

Hartung J. S. and Civerolo, E. L. 1991. Variation among strains of Xanthomonas campestris causing citrus bacterial spot. Plant Disease 75: 622-626.

Graham, J. H., J. S. Hartung, R. E. Stall and A. R. Chase. 1990. Pathological, Restriction fragment length polymorphisms, and fatty acid relationships between Xanthomonas campestris from citrus and non-citrus hosts. Phytopathology 80: 829-836.

Hartung J. S. and E. L. Civerolo. 1989. Restriction fragment length polymorphisms distinguish Xanthomonas campestris strains isolated from Florida citrus nurseries from Xanthomonas campestris pv. citri. Phytopathology 79: 793-799.

Kubicek, Q. B., E. L. Civerolo, M. R. Bonde, J. S. Hartung and G. L. Peterson. 1989. Isozyme analysis of Xanthomonas campestris pv. citri. Phytopathology 79:297-300.

Roy, A., Nandlal Choudhary, N., Hartung, J.S. and Brlansky, R. H. The prevalence of the Citrus tristeza virus trifoliate resistant breaking genotype among Puerto Rican isolates. Plant Disease. In Press. Hartung, J. S. and E. L. Civerolo. 1987.

Hartung, J. S., D. W. Fulbright and E. J. Klos. 1988. Cloning of the polysaccharide depolymerase gene of bacteriophage PEa1(h) and its expression in Erwinia amylovora. Molecular Plant-Microbe Interactions 1: 76-93.

Genomic fingerprints of Xanthomonas campestris strains from Asia, South America and Florida. Phytopathology 77: 282-285.

Hartung, J. S., D. C. Ramsdell, and C. L. Burton. 1981. Control of Alternaria fruit rot with fungicidal sprays, 1979. Fungicide and Nematicide Tests 36: 78.

Hartung, J. S., D. C. Ramsdell, and C. L. Burton. 1981. Epidemiological studies of blueberry anthracnose disease caused by Colletotrichum gloeosporioides. Phytopathology 71: 449-453.

Last Modified: 3/28/2013
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