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United States Department of Agriculture

Agricultural Research Service

Title: Transcript Nucleolytic Processing, Rna Editing, and the Restoration of Malefertility in Sorghum.

item Pring, Daryl
item Tang, Hoang
item Howad, W - RUHR UNIV, GERMANY
item Kempken, Frank - RUHR UNIV, GERMANY

Submitted to: Book Chapter
Publication Type: Book / Chapter
Publication Acceptance Date: November 1, 1998
Publication Date: N/A

Technical Abstract: Nucleolytic processing of transcripts within mitochondrial orf107, associated with male sterility in sorghum, is regulated by the fertility restoration gene Rf3, conferring 75% cleavage of whole-length transcripts. Two transcript editing sites are 81% and 61% edited in rf3rf3 lines, while these sites are 41% and 10% edited in the remaining whole-length transcripts in an Rf3Rf3 line. RNA editing and processing efficiency in F1 progeny were similar to the Rf3Rf3 parent, and analyses of backcross progeny indicated that all rf3rf3 lines were characterized by high editing efficiency. We postulate that highly edited transcripts within the population are processed in lines carrying Rf3, generating a residual population of poorly edited transcripts. Thus action of Rf3 has no direct affect on RNA editing, which may be dependent on a substrate of highly edited transcripts. These data indicate a novel role of RNA editing in gene regulation through influencing the efficiency of transcript processing.

Last Modified: 4/19/2015
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