Submitted to: Ohio Journal of Science Annual Meeting Program Abstracts
Publication Type: Abstract Only
Publication Acceptance Date: April 24, 1999
Publication Date: N/A
Technical Abstract: An unknown virus (UkV) was isolated from maize (Zea mays) leaves from Arizona showing mild mosaic symptoms and co-infected with maize dwarf mosaic virus (MDMV). A pure culture of UkV was obtained following serial transmission by vascular puncture inoculation of Seneca Chief sweetcorn kernels. Symptoms on leaves of the latter included pale green to chlorotic spots and streaks (1-5 mm) over secondary veins that developed into large spindle-shaped rings (>20 mm). The rings sometimes became necrotic and coalesced with rings from adjacent veins. Necrosis also occurred on leaf sheaths. Pioneer hybrid 3379 and 'Spirit' sweetcorn were also susceptible to the virus. UkV was not transmitted by Myzus persicae, Rhopalosiphum padi, Graminella nigrifrons or Dalbulus maidis when tested for nonpersistent or persistent transmission nor by rub inoculation of maize inbred Oh28, Triticum aestivum 'Freedom' and Sorghum bicolor 'Atlas'. UkV was purified by differential centrifugation followed by rate zonal and density gradient centrifugation in sucrose and cesium, respectively. It has an isometric particle (ca 32 nm dia), a putative 31 kDa coat protein and a RNAse sensitive nucleic acid of ca 4.3 kb. The virus did not react with antisera to MDMV, maize chlorotic dwarf, maize stripe or maize white line mosaic viruses in F(ab )2 ELISA. UkV is conditionally named maize necrotic streak virus.