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ARS Home » Midwest Area » Madison, Wisconsin » Vegetable Crops Research » Research » Publications at this Location » Publication #95294
Title: PHYLOGENY AND RELATIONSHIPS IN DAUCUS BASED ON RESTRICTION FRAGMENT LENGTH POLYMORPHISMS (RFLPS) OF THE CHLOROPLAST AND MITOCHONDRIAL GENOMES

Author
item VIVEK, B S - DEPT OF HORT UW MADISON
item Simon, Philipp

Submitted to: Journal of Euphytica
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 4/14/1998
Publication Date: N/A
Citation: N/A

Interpretive Summary: Genetic relationships among carrot and its wild relatives can be elucidated by an evaluation of DNA from different parts of the plant cell, namely the mitochondria and plastid. Mitochondrial DNA was highly variable in contrast to plasted DNA which was very uniform. Both plant parts, however, gave results which were similar to each other. These results also compared well with the classical method for determining genetic relationships, namely by visual comparisons of these plants. This research has a potential positive impact for carrot geneticists and breeders attempting to intercross carrots.

Technical Abstract: Chloroplast DNA (cpDNA) restriction site analysis of nine species of Daucus (covering four of five sections) and nine carrot accessions was done using 14 Petunia cpDNA clones as probes and 10 restriction enzymes. Cladistic analysis yielded a phylogent generally concordant with a recent morphological classification. A cpDNA insertion deletion mutation was found in the cultivated carrots (probe P10 Bgl ll digest). Mitochondrial DNA (mtDNA) restriction site analysis of the same material using four maize mtDNA cones as probes and 10 enzymes exhibited extensive mitochondrial diversity in Daucus, both within and between sections.