Technical Abstract: A strain belonging to the genus Leptospira serogroup Bataviae, isolated from an ox at slaughter in Zimbabwe, was identified by using cross-agglutin absorption, monoclonal antibodies, restriction fragment length polymorphism, and polymerase chain reaction analyses. Results of both serological tests showed that the isolate (strain SBF 37) was antigenically yvery similar to reference strain Paidjan and, therefore, belongs to serova paidjan. Strain SBF 37 was, however, genetically different from strain Paidjan since their chromosomal DNA had different restriction fragment length polymorphism patterns, and as the Zimbabwe serovar paidjin isolate was identified as belonging to Leptospira kirschneri, while the reference strain for the serovar, strain Paidjin, belonged to a species other than L. kirschneri as determined by the polymerase chain reaction.