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ARS Home » Midwest Area » St. Paul, Minnesota » Plant Science Research » Research » Publications at this Location » Publication #93575
Title: EARLY DETECTION OF CHROMOSOME RETENTION/ELIMINATION IN OAT X MAIZE HYBRIDS USING GENOMIC IN SITU HYBRIDIZATION

Author
item ANANIEV, EVGUENI - UNIVERSITY OF MINNESOTA
item CHEN, GANG - UNIVERSITY OF MINNESOTA
item Rines, Howard
item PHILLIPS, RONALD - UNIVERSITY OF MINNESOTA

Submitted to: American Oat Workers Conference Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: 7/27/1998
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: Interspecific hybridization between different species of cereals is widely used for the production of haploid plants or chromosome addition lines as the result of preferential elimination or occasional retention of chromosomes of one of the parents. The factors which affect chromosome elimination/retention are poorly understood and difficult to monitor. We applied genomic fluorescence in situ hybridization for the early detection of chromosome elimination/retention in developing embryos, endosperm, and early seedlings in oat x maize hybrids. Developing embryos and endosperm were isolated for analysis from caryopses at 3 and 6 days after pollination. In addition, root tips of young seedlings from rescued embryos were cut off, fixed in 45% acetic acid, squashed, and made into permanent preparations. Maize genomic DNA or a multiprobe were labeled with fluorescein and used for in situ hybridization. Maize chromosomal material was detected in metaphase plates as well as in interphase nuclei using thi technique. All embryos from oat-maize crosses containing maize chromosomes were chimeric. Different cells in a developing embryo can have different numbers of retained maize chromosomes from zero to ten. In older embryos it is possible to detect sectors of cells without maize chromosomes. Most endosperm cells in contrast contain practically a full set of maize chromosomes. The cell division in endosperm usually ceases early in development. Different levels of chromosome retention/elimination were detected in developing young seedlings. The process of maize chromosome elimination appears to begin during the first cell divisions after fertilization, occurs with different rates in different tissues, and is highly asynchronous.