Skip to main content
ARS Home » Midwest Area » St. Paul, Minnesota » Plant Science Research » Research » Publications at this Location » Publication #93570
Title: DEVELOPMENT OF A 'SUN II' OAT ANEUPLOID SERIES FOR RFLP MARKER ASSOCIATIONSTO CHROMOSOME

Author
item START, MARYANN - UNIVERSITY OF MINNESOTA
item Rines, Howard
item PHILLIPS, RONALD - UNIVERSITY OF MINNESOTA

Submitted to: American Oat Workers Conference Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: 7/27/1998
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: The molecular genetic linkage map for cultivated hexaploid oat (2n=6x=42) constructed from an Avena byzantina cv. Kanota by A. sativa cv. Ogle cross using principally RFLP markers consists of at least 532 loci assigned to 38 linkage groups. Additional mapping efforts and the use of aneuploids have lowered the number of linkage groups to 33. The objective of this research his to reduce the number of linkage groups to 21, the expected number for hexaploid oat, by assigning linkage groups and unlinked markers to physical chromosome using a nearly complete Sun II monosomic series. A series of 20 oat monosomics has been assembled, with each missing a different chromosome based on C-banding analysis. Fourteen of the monosomics were newly derived from Sun II oat haploids generated in oat by maize crosses. Three of the monosomics were derived by Hacker and Riley in a different selection of Sun II and have a 3C-14 interchange. Backcrosses have been made in an attempt to eliminate the interchange and to standardize the background of the Sun II monosomics. Three monosomics have only been recovered in the cultivar Kanota, as described by Morikawa. Backcrosses with Sun II have been made with these three Kanota monosomics. Each monosomic line available was crossed with Kanota and Ogle. Monosomic F1s from these crosses were identified by mitotic chromosome counts of root tips, and leaf tissue was collected for DNA extraction. RFLP autoradiograms will be analyzed and missing bands will allow assignment of sequences to the monosomic chromosomes.