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ARS Home » Midwest Area » West Lafayette, Indiana » Crop Production and Pest Control Research » Research » Publications at this Location » Publication #93428
Title: AGROBACTERIUM-MEDIATED TRANSFORMATION OF SORGHUM

Author
item CARVALHO, C H S - PURDUE UNIVERSITY
item ZEHR, U B - PURDUE UNIVERSITY
item GUNARATNA, N - PURDUE UNIVERSITY
item KONONOWICZ, H - PURDUE UNIVERSITY
item Anderson, Joseph
item HODGES, T K - PURDUE UNIVERSITY
item AXTELL, J D - PURDUE UNIVERSITY

Submitted to: American Society of Agronomy Meetings
Publication Type: Abstract Only
Publication Acceptance Date: 7/29/1998
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: Sorghum transgenic plants were produced from immature embryos co-cultivated with Agrobacterium tumefaciens LBA4404 (pTOK233). Stable integration and inheritance of the transgenes was demonstrated by Southern blot analysis of transformants in R0 and R1 generations. We tested freshly isolated immature embryos, pre-cultured immature embryos, immature inflorescence, and callus as explants for transformation. Freshly isolated immature embryos were chosen because they usually showed the highest transient expression and allowed better monitoring of the transformation events. Explant recovery after co-cultivation was a limiting step during the transformation procedure. We found that the combination of coconut water in the co-cultivation medium and actively growing immature embryos, which allowed callus formation after co-cultivation, were key factors for successful transformation. The efficiency of Agrobacterium-mediated transformation of sorghum is still very low, nevertheless our present data indicates that it is possible and can be improved.