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ARS Home » Southeast Area » New Orleans, Louisiana » Southern Regional Research Center » Cotton Structure and Quality Research » Research » Publications at this Location » Publication #92662
Title: CELL WALL SUBUNITS, "GLUE" MATRIX AND COTTON FIBER DEVELOPMENT

Author
item MURRAY, A - GLYCOZYME, INC
item Sassenrath, Gretchen
item Bradow, Judith

Submitted to: World Cotton Research Conference Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: 3/21/1999
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: The object of this study was to isolate and characterize the cell wall subunits and "glue" matrix involved in cell wall synthesis in developing cotton fibers. This study employed fibers taken from 25 DPA bolls collected at 7 a.m., noon and 7 p.m. Cold aqueous extracts of developing cotton were by HPAEC-PAD (Murray, 1998). Subsequent sequential extracts were taken on a daily basis at three temperatures, 37 degrees, room temperature, and 4 degrees. Extracts were fractionated by centrifugation and filtration. The fractions of the extracts were subjected to alkaline borohydride, mild acid, protease digestion or glycosidase treatment followed by PAGE and HPAEC-PAD (Murray, 1998). The cell wall subunits and "glue" matrix were found to vary both in the quantity extracted and in the character of the "glue" matrix extracted. This variability is dependent both on the time of day which the fibers were collected and the temperature eof the extraction. The extracts demonstrated the ability to synthesize th "glue" matrix by a temperature dependent mechanism, which "consumed" hexoses in the process and released apparent carriers. The "glue" matrix appears to add carbohydrate residues to the subunits in the matrix after the initial carbohydrate residues are conjugated. The enzymatic activities associated with the "glue" matrix will be discussed. (Murray, A.K., 1998, U.S. Patent No. 5,710,047)