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United States Department of Agriculture

Agricultural Research Service

Title: High-Performance Capillary Electrophoresis of Cereal Proteins

Authors
item Bean, S - KANSAS STATE UNIVERSITY
item Bietz, Jerold
item Lookhart, George

Submitted to: Journal of Chromatography
Publication Type: Review Article
Publication Acceptance Date: July 21, 1998
Publication Date: N/A

Interpretive Summary: Review chapter - no interpretive summary required.

Technical Abstract: Cereal grains are widely used for human foods and animal feeds throughout the world. Cereals provide dietary protein, which also often has a functional role, as wheat gluten does in bread. Cereal proteins are unique in many ways: they are highly complex and heterogeneous, are often difficult to extract, and aggregate readily, making them difficult to characterize. Because of the economic importance and widespread use of cereal proteins, however, many techniques have been used for their analysis. High-performance capillary electrophoresis (HPCE) is one of the newest techniques to be so used. This review describes the development of charge- and size-based HPCE methods for analysis of cereal grain proteins, and the use of these methods for cultivar identification, classification, and prediction of quality. HPCE is versatile, rapid, easily automated, readily quantified, and provides high-resolution separations. Clearly, HPCE is a valuable addition to other methods of cereal protein analysis and should, in time, be applicable to all protein classes from all cereals.

Last Modified: 7/30/2014
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