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United States Department of Agriculture

Agricultural Research Service

Title: Retrospective Comparison of Histopathology and Immunohistochemistry for Diagnosing Ovine Scrapie

item Jenny, Allen - APHIS,NVSL,AMES,IOWA
item Taylor, William - APHIS,NVSL,AMES,IOWA
item Miller, Janice
item Davis, Arthur - APHIS,NVSL,AMES,IOWA

Submitted to: American Association of Veterinary Laboratory Diagnosticians
Publication Type: Abstract Only
Publication Acceptance Date: July 10, 1998
Publication Date: N/A

Technical Abstract: Histopathologic diagnoses were compared with the results obtained by hydrated autoclaving immunohistochemistry on brain for the abnormal form of the prion protein (PrP**res) on samples from more than 350 sheep showing clinical signs suggestive of CNS disease or progressive weight loss. When histopathology findings were consistent with scrapie, immunohisto- chemistry results were positive in over 99 percent of the cases. When histopathology findings were suggestive of scrapie, immunohistochemistry results were positive for PrP**res in over 80 percent of the animals. In cases where no diagnosis was established but some lesions characteristic of scrapie were found, i.e., vacuolated neurons and/or spongiform change in the grey matter neuropil, immunohistochemistry results were positive for PrP**res in over 50 percent of animals. The significance of the findings in a scrapie control or eradication program will be discussed.

Last Modified: 4/1/2015
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