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United States Department of Agriculture

Agricultural Research Service

Title: Rapid Identification of Phytophthora Species That Parasitize Potatoes Usingsingle Nucleotide Primer Extension (Snupe) Analysis.

item Scott, David
item Deahl, Kenneth

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: December 30, 1998
Publication Date: N/A

Technical Abstract: The genus Phytophthora contains at least 3 species that parasitize potatoes. The rapid and specific identification of these species is very important in developing pest management strategies. However, differentiation of members of this genus has been quite difficult because of the high degree of variability of morphological and physiological characters, which often overlap between species. A region containing species-specific point mutations within the internal transcribed spacer 1 (ITS1) region of Phytophthora species that parasitize potatoes was targeted for PCR using genus specific primers. The resulting amplicon was used as template in a non-radioactive single nucleotide primer extension (SNuPE) assay to distinguish P. infestans from P. erythroseptica and P. nicotianae. The method is simple, specific, and highly quantitative for determining disease progression. The technique has been modified to include an inter-sample normalizer, and adaptation of the SnuPE assay to other plant pathogens of interest to plant pathologist should be easy to achieve.

Last Modified: 4/22/2015
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