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ARS Home » Plains Area » Fargo, North Dakota » Edward T. Schafer Agricultural Research Center » Sugarbeet and Potato Research » Research » Publications at this Location » Publication #88941
Title: A TWO-WAY APPROACH TO IMPROVE ROOT ROT RESISTANCE

Author
item BUGBEE, WILLIAM - RETIRED ARS
item WOZNIAK, CHRIS - FORMER ARS
item Smith, Garry

Submitted to: American Society of Sugarbeet Technologists
Publication Type: Proceedings
Publication Acceptance Date: 3/9/1995
Publication Date: N/A
Citation: N/A

Interpretive Summary: Pectin lyase, produced by Rhizoctonia solani, was found associated with crown and root rot on sugarbeet. The sugar beet also was found to produce a pectin lyase inhibitor protein (PNLIP). Plants with high or low levels of PNLIP were cloned by apical meristem culture. Clones were interpollinated to create four synthetic lines. In a small greenhouse trial, the effect of this selection technique was not conclusive.

Technical Abstract: Pectin lyase, produced by Rhizoctonia solani, was found associated with crown and root rot on sugar beet. The sugar beet also was found to produce a pectin lyase inhibitor protein (PNLIP). The behavior of PNLIP in controlled experiments prompted efforts to pursue the goal of manipulating PNLIP for enhanced root rot resistance. Polyclonal and monoclonal antibodies to PNLIP were used to probe sugar beet CDNA libraries. Transformed E. coli colonies were lifted with nitrocellulose membranes, lysed directly on the membranes and probled with antibodies. The monoclonals appeared to be more specific than the polyclonals. Colonies whose lysates reacted positively with monoclonals, were electrophoresed and the protein bands were electroblotted to nitrocellulose. None of these fractionated bands reacted with the monoclonals. Nine amino acids at the amino acid sequence and will be used in further efforts to isoalte the PNLIP-encloding DNA. The polyclonal and monoclonal antibodies were used in a double antibody sandwich ELISA protocol to estimate PNLIP content in small samples of sugar beet extract. Plants with high or low levels of PNLIP were clones by apical meristem culture. Clones were interpollinated to create four synthetic lines. In a small greenhouse trial, the effet of this selection technique was not conclusive.