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United States Department of Agriculture

Agricultural Research Service

Title: Genetic Mapping of the Fr1 (Root Fluorescence) Locus in Soybean (Gllycine Max (L) MERR.) Using Ssr Markers and Bulked Segregant Analysis

item Palmer, Reid
item Horner, Harry - IOWA STATE UNIVERSITY
item Shoemaker, Randy

Submitted to: Journal of Heredity
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: September 15, 1998
Publication Date: N/A

Interpretive Summary: In plants and animals genetic traits can be independent of each other or they can be associated. Genetic linkage is the term used to describe associated traits. In soybean our goal was to correlate linked traits that have been characterized using traditional genetic techniques with linked traits determined by biochemical techniques. We were successful. The trait controlling root color viewed under ultraviolet light was correlated to molecular maps. This association will add to our knowledge of making a road map of linked traits in soybean. Knowledge of more linked traits will aid plant breeders in providing better soybeans for the farmer.

Technical Abstract: We report the use of bulked segregant SSR analysis for rapid identification of DNA markers linked to the Fr1 locus in soybean. Pooled DNA extracts from 10 homozygous Fr1 Fr1 and 10 fr1 fr1 F2 plants, derived from a msMOS X Minsoy cross, were analyzed using 65 SSR markers. Five SSRs produced repeatable polymorphisms between paired bulks. Linkage with the Fr1 locus then was tested using these five SSR primers and DNA from individual plant of each bulk. DNA polymorphisms generated by these five primers were linked to the Fr1 gene. Linkage of SSR loci with the Fr1 locus was verified by using an F2 population segregating for Fr1. The five SSR markers and Fr1 are on linkage group K of the USDA/ARS/ISU molecular genetic map. The markers flanking Fr1 an d Satt337 (11.0 cM) and Sat 044 (0.6 cM). Fr1 previously was mapped on linkage group 12 of the classical genetic map. Thus, classical genetic linkage group 12 has been correlated to linkage group K of the molecular genetic map. Six SSR markers were chosen on linkage group K to test the segregation ratio. All six SSRs consistently showed distorted segregation ratios, three of them were significantly skewed. This suggested that a gametophyte factor may lie in the region close to Fr1 and most likely close to Satt046.

Last Modified: 4/21/2015
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