Page Banner

United States Department of Agriculture

Agricultural Research Service

Title: Characterization of the 1b/1r Translocation in Winter Wheat Cultivars by Polyacrylamide Gel Electrophoresis and Capillary Electrophoresis

Authors
item Waga, Jacek - PLANT BREEDING & ACCLIMTZ
item Bietz, Jerold

Submitted to: Plant Breeding and Seed Science
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: December 4, 1997
Publication Date: N/A

Interpretive Summary: Adding certain rye genes to wheat increases yield and disease resistance, but can adversely affect wheat quality. These rye genes code blocks of secalin storage proteins that can be detected by gel electrophoresis or by newer techniques. Earlier, capillary electrophoresis (CE) showed different amounts of storage proteins in some wheats having the same protein blocks. We therefore now used CE to measure amounts of secalins in wheats having the same proteins but differing in quality. Amounts of these proteins were closely related to quality. CE may thus be a useful selection tool during breeding to identify genetic variants with modified protein compositions and improved properties.

Technical Abstract: Polyacrylamide gel electrophoresis reveals expression of wheat storage proteins coded by 1B/1R translocated rye genes as a group of secalin bands known (in the catalogue of Metakovsky) as the Gli B1 l protein block. Ten genotypes that contained this block but differed in technological properties (as predicted by sedimentation value) were analyzed by high performance capillary electrophoresis (HPCE). Quantitative variation among genotypes was found for proteins in this block. Heights of peaks corresponding to the Gli B1 l block correlated significantly with sedimentation values. HPCE may prove to be a valuable selection tool during breeding for modifying and improving technological properties.

Last Modified: 4/20/2014
Footer Content Back to Top of Page