ARS | Publication request: IDENTIFICATION OF FOUR HIGHLY POLYMORPHIC PORCINE MICROSATELLITE LOCI

Submitted to: Journal of Animal Science
Publication Type: Abstract Only
Publication Acceptance Date: November 6, 1998
Publication Date: N/A

Technical Abstract: Source of clones: Porcine genomic DNA library was screened for microsatellites as described by Stone et al. (1997). Primers were developed as described by Rohrer et al. (1994). Description of polymorphisms: Markers were genotyped in the MARC swine reference population (Rohrer et al., 1994) and polymorphisms were evident in the nine parents tested. Mendelian inheritance of each allele was confirmed in seven fullsib families of the MARC swine reference population. Chromosome location: Twopoint linkage analyses were conducted with CRI-MAP 2.4 (Green et al., 1990) between these markers and all markers present in the MARC genome database. The most significant linkages detected and the most likely position of each marker based on multipoint linkage analyses is presented in Table 1. Comments: These markers may be useful to scientists mapping QTL in pigs. All markers were easy to score and a minimum of five unique alleles were identified in nine pigs. Most of these markers may be quite useful in resource populations to identify QTL.